[Cloning and characterization of an aldehyde dehydrogenase gene GmALDH3-1 from soybean].
Identifieur interne : 003186 ( Main/Corpus ); précédent : 003185; suivant : 003187[Cloning and characterization of an aldehyde dehydrogenase gene GmALDH3-1 from soybean].
Auteurs : Fang Huang ; Ying-Jun Chi ; Hui He ; De-Yue YuSource :
- Yi chuan = Hereditas [ 0253-9772 ] ; 2010.
English descriptors
- KwdEn :
- Aldehyde Dehydrogenase (classification), Aldehyde Dehydrogenase (genetics), Amino Acid Sequence (MeSH), Cloning, Molecular (MeSH), DNA, Complementary (chemistry), DNA, Complementary (genetics), Flowers (genetics), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plant Leaves (genetics), Plant Proteins (genetics), Plant Roots (genetics), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Sequence Analysis, DNA (MeSH), Sequence Homology, Amino Acid (MeSH), Soybean Proteins (genetics), Soybeans (enzymology), Soybeans (genetics).
- MESH :
- chemical , chemistry : DNA, Complementary.
- chemical , classification : Aldehyde Dehydrogenase.
- chemical , genetics : Aldehyde Dehydrogenase, DNA, Complementary, Plant Proteins, Soybean Proteins.
- enzymology : Soybeans.
- genetics : Flowers, Plant Leaves, Plant Roots, Soybeans.
- Amino Acid Sequence, Cloning, Molecular, Gene Expression Profiling, Gene Expression Regulation, Plant, Molecular Sequence Data, Phylogeny, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Homology, Amino Acid.
Abstract
To research the mechanism of soybean reproductive development, we identified a number of flower development related genes in soybean by microarray hybridization. A gene predominately expressed in soybean flowers was chosen for further analysis. Through bioinformatic and RT-PCR approaches, the full-length gene was cloned from soybean flowers. The results of BLAST searching indicated that this gene encoded for an aldehyde dehydrogenase and was named as GmALDH3-1. GmALDH3-1 contains a complete open reading frame of 1485 bp in length, which encodes for a peptide of 494 amino acids. The product encoded by GmALDH3-1 shows 83% similarity and 68% identity with Populus tomentosa PtALDH3, respectively, and 39% and 59% with human ALDH3B. Phylogenetic analysis shows that GmALDH3-1 and other ALDH3 subfamily members are grouped into the same branch and GmALDH3-1 is close to PtALDH3 and Arabidopsis AtALDH3F1. Real-time RT-PCR analysis demonstrated that the highest expression level of GmALDH3-1 occurred in flowers, but the expression of this gene was almost undetectable in leaves and roots. We further analyzed GmALDH3-1 expression during the course of seed development based on publicly available microarray data and found that GmALDH3-1 was highly expressed in the seed endothelium, epidermis, outer integument, and hilum.
DOI: 10.3724/sp.j.1005.2010.00492
PubMed: 20466639
Links to Exploration step
pubmed:20466639Le document en format XML
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<author><name sortKey="Huang, Fang" sort="Huang, Fang" uniqKey="Huang F" first="Fang" last="Huang">Fang Huang</name>
<affiliation><nlm:affiliation>National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. fhuang@njau.edu.cn</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Chi, Ying Jun" sort="Chi, Ying Jun" uniqKey="Chi Y" first="Ying-Jun" last="Chi">Ying-Jun Chi</name>
</author>
<author><name sortKey="He, Hui" sort="He, Hui" uniqKey="He H" first="Hui" last="He">Hui He</name>
</author>
<author><name sortKey="Yu, De Yue" sort="Yu, De Yue" uniqKey="Yu D" first="De-Yue" last="Yu">De-Yue Yu</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Cloning and characterization of an aldehyde dehydrogenase gene GmALDH3-1 from soybean].</title>
<author><name sortKey="Huang, Fang" sort="Huang, Fang" uniqKey="Huang F" first="Fang" last="Huang">Fang Huang</name>
<affiliation><nlm:affiliation>National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. fhuang@njau.edu.cn</nlm:affiliation>
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<author><name sortKey="Chi, Ying Jun" sort="Chi, Ying Jun" uniqKey="Chi Y" first="Ying-Jun" last="Chi">Ying-Jun Chi</name>
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<author><name sortKey="He, Hui" sort="He, Hui" uniqKey="He H" first="Hui" last="He">Hui He</name>
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<author><name sortKey="Yu, De Yue" sort="Yu, De Yue" uniqKey="Yu D" first="De-Yue" last="Yu">De-Yue Yu</name>
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<series><title level="j">Yi chuan = Hereditas</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aldehyde Dehydrogenase (classification)</term>
<term>Aldehyde Dehydrogenase (genetics)</term>
<term>Amino Acid Sequence (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>DNA, Complementary (chemistry)</term>
<term>DNA, Complementary (genetics)</term>
<term>Flowers (genetics)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Phylogeny (MeSH)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Roots (genetics)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
<term>Soybean Proteins (genetics)</term>
<term>Soybeans (enzymology)</term>
<term>Soybeans (genetics)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Aldehyde Dehydrogenase</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Aldehyde Dehydrogenase</term>
<term>DNA, Complementary</term>
<term>Plant Proteins</term>
<term>Soybean Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Soybeans</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Flowers</term>
<term>Plant Leaves</term>
<term>Plant Roots</term>
<term>Soybeans</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Cloning, Molecular</term>
<term>Gene Expression Profiling</term>
<term>Gene Expression Regulation, Plant</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Homology, Amino Acid</term>
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<front><div type="abstract" xml:lang="en">To research the mechanism of soybean reproductive development, we identified a number of flower development related genes in soybean by microarray hybridization. A gene predominately expressed in soybean flowers was chosen for further analysis. Through bioinformatic and RT-PCR approaches, the full-length gene was cloned from soybean flowers. The results of BLAST searching indicated that this gene encoded for an aldehyde dehydrogenase and was named as GmALDH3-1. GmALDH3-1 contains a complete open reading frame of 1485 bp in length, which encodes for a peptide of 494 amino acids. The product encoded by GmALDH3-1 shows 83% similarity and 68% identity with Populus tomentosa PtALDH3, respectively, and 39% and 59% with human ALDH3B. Phylogenetic analysis shows that GmALDH3-1 and other ALDH3 subfamily members are grouped into the same branch and GmALDH3-1 is close to PtALDH3 and Arabidopsis AtALDH3F1. Real-time RT-PCR analysis demonstrated that the highest expression level of GmALDH3-1 occurred in flowers, but the expression of this gene was almost undetectable in leaves and roots. We further analyzed GmALDH3-1 expression during the course of seed development based on publicly available microarray data and found that GmALDH3-1 was highly expressed in the seed endothelium, epidermis, outer integument, and hilum.</div>
</front>
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<DateCompleted><Year>2010</Year>
<Month>12</Month>
<Day>10</Day>
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<DateRevised><Year>2019</Year>
<Month>09</Month>
<Day>23</Day>
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<ArticleTitle>[Cloning and characterization of an aldehyde dehydrogenase gene GmALDH3-1 from soybean].</ArticleTitle>
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<Abstract><AbstractText>To research the mechanism of soybean reproductive development, we identified a number of flower development related genes in soybean by microarray hybridization. A gene predominately expressed in soybean flowers was chosen for further analysis. Through bioinformatic and RT-PCR approaches, the full-length gene was cloned from soybean flowers. The results of BLAST searching indicated that this gene encoded for an aldehyde dehydrogenase and was named as GmALDH3-1. GmALDH3-1 contains a complete open reading frame of 1485 bp in length, which encodes for a peptide of 494 amino acids. The product encoded by GmALDH3-1 shows 83% similarity and 68% identity with Populus tomentosa PtALDH3, respectively, and 39% and 59% with human ALDH3B. Phylogenetic analysis shows that GmALDH3-1 and other ALDH3 subfamily members are grouped into the same branch and GmALDH3-1 is close to PtALDH3 and Arabidopsis AtALDH3F1. Real-time RT-PCR analysis demonstrated that the highest expression level of GmALDH3-1 occurred in flowers, but the expression of this gene was almost undetectable in leaves and roots. We further analyzed GmALDH3-1 expression during the course of seed development based on publicly available microarray data and found that GmALDH3-1 was highly expressed in the seed endothelium, epidermis, outer integument, and hilum.</AbstractText>
</Abstract>
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<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
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