Transgenic poplar expressing Arabidopsis NDPK2 enhances growth as well as oxidative stress tolerance.
Identifieur interne : 003118 ( Main/Corpus ); précédent : 003117; suivant : 003119Transgenic poplar expressing Arabidopsis NDPK2 enhances growth as well as oxidative stress tolerance.
Auteurs : Yun-Hee Kim ; Myoung Duck Kim ; Young Im Choi ; Sung-Chul Park ; Dae-Jin Yun ; Eun Woon Noh ; Haeng-Soon Lee ; Sang-Soo KwakSource :
- Plant biotechnology journal [ 1467-7652 ] ; 2011.
English descriptors
- KwdEn :
- Adaptation, Physiological (drug effects), Arabidopsis (drug effects), Arabidopsis (enzymology), Gene Expression Regulation, Plant (drug effects), Genes, Plant (genetics), Hydrogen Peroxide (metabolism), Nucleoside-Diphosphate Kinase (metabolism), Oxidative Stress (drug effects), Paraquat (pharmacology), Photosynthesis (drug effects), Photosynthesis (genetics), Plants, Genetically Modified (MeSH), Populus (drug effects), Populus (enzymology), Populus (genetics), Populus (growth & development), Stress, Physiological (drug effects).
- MESH :
- chemical , metabolism : Hydrogen Peroxide, Nucleoside-Diphosphate Kinase.
- drug effects : Adaptation, Physiological, Arabidopsis, Gene Expression Regulation, Plant, Oxidative Stress, Photosynthesis, Populus, Stress, Physiological.
- enzymology : Arabidopsis, Populus.
- genetics : Genes, Plant, Photosynthesis, Populus.
- growth & development : Populus.
- chemical , pharmacology : Paraquat.
- Plants, Genetically Modified.
Abstract
Nucleoside diphosphate kinase 2 (NDPK2) is known to regulate the expression of antioxidant genes in plants. Previously, we reported that overexpression of Arabidopsis NDPK2 (AtNDPK2) under the control of an oxidative stress-inducible SWPA2 promoter in transgenic potato and sweetpotato plants enhanced tolerance to various abiotic stresses. In this study, transgenic poplar (Populus alba × Poplus glandulosa) expressing the AtNDPK2 gene under the control of a SWPA2 promoter (referred to as SN) was generated to develop plants with enhanced tolerance to oxidative stress. The level of AtNDPK2 expression and NDPK activity in SN plants following methyl viologen (MV) treatment was positively correlated with the plant's tolerance to MV-mediated oxidative stress. We also observed that antioxidant enzyme activities such as ascorbate peroxidase, catalase and peroxidase were increased in MV-treated leaf discs of SN plants. The growth of SN plants was substantially increased under field conditions including increased branch number and stem diameter. SN plants exhibited higher transcript levels of the auxin-response genes IAA2 and IAA5. These results suggest that enhanced AtNDPK2 expression affects oxidative stress tolerance leading to improved plant growth in transgenic poplar.
DOI: 10.1111/j.1467-7652.2010.00551.x
PubMed: 20649941
Links to Exploration step
pubmed:20649941Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transgenic poplar expressing Arabidopsis NDPK2 enhances growth as well as oxidative stress tolerance.</title><author><name sortKey="Kim, Yun Hee" sort="Kim, Yun Hee" uniqKey="Kim Y" first="Yun-Hee" last="Kim">Yun-Hee Kim</name><affiliation><nlm:affiliation>Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Myoung Duck" sort="Kim, Myoung Duck" uniqKey="Kim M" first="Myoung Duck" last="Kim">Myoung Duck Kim</name></author><author><name sortKey="Choi, Young Im" sort="Choi, Young Im" uniqKey="Choi Y" first="Young Im" last="Choi">Young Im Choi</name></author><author><name sortKey="Park, Sung Chul" sort="Park, Sung Chul" uniqKey="Park S" first="Sung-Chul" last="Park">Sung-Chul Park</name></author><author><name sortKey="Yun, Dae Jin" sort="Yun, Dae Jin" uniqKey="Yun D" first="Dae-Jin" last="Yun">Dae-Jin Yun</name></author><author><name sortKey="Noh, Eun Woon" sort="Noh, Eun Woon" uniqKey="Noh E" first="Eun Woon" last="Noh">Eun Woon Noh</name></author><author><name sortKey="Lee, Haeng Soon" sort="Lee, Haeng Soon" uniqKey="Lee H" first="Haeng-Soon" last="Lee">Haeng-Soon Lee</name></author><author><name sortKey="Kwak, Sang Soo" sort="Kwak, Sang Soo" uniqKey="Kwak S" first="Sang-Soo" last="Kwak">Sang-Soo Kwak</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:20649941</idno><idno type="pmid">20649941</idno><idno type="doi">10.1111/j.1467-7652.2010.00551.x</idno><idno type="wicri:Area/Main/Corpus">003118</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003118</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Transgenic poplar expressing Arabidopsis NDPK2 enhances growth as well as oxidative stress tolerance.</title><author><name sortKey="Kim, Yun Hee" sort="Kim, Yun Hee" uniqKey="Kim Y" first="Yun-Hee" last="Kim">Yun-Hee Kim</name><affiliation><nlm:affiliation>Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Myoung Duck" sort="Kim, Myoung Duck" uniqKey="Kim M" first="Myoung Duck" last="Kim">Myoung Duck Kim</name></author><author><name sortKey="Choi, Young Im" sort="Choi, Young Im" uniqKey="Choi Y" first="Young Im" last="Choi">Young Im Choi</name></author><author><name sortKey="Park, Sung Chul" sort="Park, Sung Chul" uniqKey="Park S" first="Sung-Chul" last="Park">Sung-Chul Park</name></author><author><name sortKey="Yun, Dae Jin" sort="Yun, Dae Jin" uniqKey="Yun D" first="Dae-Jin" last="Yun">Dae-Jin Yun</name></author><author><name sortKey="Noh, Eun Woon" sort="Noh, Eun Woon" uniqKey="Noh E" first="Eun Woon" last="Noh">Eun Woon Noh</name></author><author><name sortKey="Lee, Haeng Soon" sort="Lee, Haeng Soon" uniqKey="Lee H" first="Haeng-Soon" last="Lee">Haeng-Soon Lee</name></author><author><name sortKey="Kwak, Sang Soo" sort="Kwak, Sang Soo" uniqKey="Kwak S" first="Sang-Soo" last="Kwak">Sang-Soo Kwak</name></author></analytic><series><title level="j">Plant biotechnology journal</title><idno type="eISSN">1467-7652</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation, Physiological (drug effects)</term><term>Arabidopsis (drug effects)</term><term>Arabidopsis (enzymology)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Genes, Plant (genetics)</term><term>Hydrogen Peroxide (metabolism)</term><term>Nucleoside-Diphosphate Kinase (metabolism)</term><term>Oxidative Stress (drug effects)</term><term>Paraquat (pharmacology)</term><term>Photosynthesis (drug effects)</term><term>Photosynthesis (genetics)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (drug effects)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Stress, Physiological (drug effects)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hydrogen Peroxide</term><term>Nucleoside-Diphosphate Kinase</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Adaptation, Physiological</term><term>Arabidopsis</term><term>Gene Expression Regulation, Plant</term><term>Oxidative Stress</term><term>Photosynthesis</term><term>Populus</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Arabidopsis</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, Plant</term><term>Photosynthesis</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Paraquat</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Plants, Genetically Modified</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nucleoside diphosphate kinase 2 (NDPK2) is known to regulate the expression of antioxidant genes in plants. Previously, we reported that overexpression of Arabidopsis NDPK2 (AtNDPK2) under the control of an oxidative stress-inducible SWPA2 promoter in transgenic potato and sweetpotato plants enhanced tolerance to various abiotic stresses. In this study, transgenic poplar (Populus alba × Poplus glandulosa) expressing the AtNDPK2 gene under the control of a SWPA2 promoter (referred to as SN) was generated to develop plants with enhanced tolerance to oxidative stress. The level of AtNDPK2 expression and NDPK activity in SN plants following methyl viologen (MV) treatment was positively correlated with the plant's tolerance to MV-mediated oxidative stress. We also observed that antioxidant enzyme activities such as ascorbate peroxidase, catalase and peroxidase were increased in MV-treated leaf discs of SN plants. The growth of SN plants was substantially increased under field conditions including increased branch number and stem diameter. SN plants exhibited higher transcript levels of the auxin-response genes IAA2 and IAA5. These results suggest that enhanced AtNDPK2 expression affects oxidative stress tolerance leading to improved plant growth in transgenic poplar.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20649941</PMID><DateCompleted><Year>2011</Year><Month>09</Month><Day>07</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1467-7652</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>3</Issue><PubDate><Year>2011</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Plant biotechnology journal</Title><ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>Transgenic poplar expressing Arabidopsis NDPK2 enhances growth as well as oxidative stress tolerance.</ArticleTitle><Pagination><MedlinePgn>334-47</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1467-7652.2010.00551.x</ELocationID><Abstract><AbstractText>Nucleoside diphosphate kinase 2 (NDPK2) is known to regulate the expression of antioxidant genes in plants. Previously, we reported that overexpression of Arabidopsis NDPK2 (AtNDPK2) under the control of an oxidative stress-inducible SWPA2 promoter in transgenic potato and sweetpotato plants enhanced tolerance to various abiotic stresses. In this study, transgenic poplar (Populus alba × Poplus glandulosa) expressing the AtNDPK2 gene under the control of a SWPA2 promoter (referred to as SN) was generated to develop plants with enhanced tolerance to oxidative stress. The level of AtNDPK2 expression and NDPK activity in SN plants following methyl viologen (MV) treatment was positively correlated with the plant's tolerance to MV-mediated oxidative stress. We also observed that antioxidant enzyme activities such as ascorbate peroxidase, catalase and peroxidase were increased in MV-treated leaf discs of SN plants. The growth of SN plants was substantially increased under field conditions including increased branch number and stem diameter. SN plants exhibited higher transcript levels of the auxin-response genes IAA2 and IAA5. These results suggest that enhanced AtNDPK2 expression affects oxidative stress tolerance leading to improved plant growth in transgenic poplar.</AbstractText><CopyrightInformation>© 2010 The Authors. Plant Biotechnology Journal © 2010 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Yun-Hee</ForeName><Initials>YH</Initials><AffiliationInfo><Affiliation>Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Myoung Duck</ForeName><Initials>MD</Initials></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Young Im</ForeName><Initials>YI</Initials></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Sung-Chul</ForeName><Initials>SC</Initials></Author><Author ValidYN="Y"><LastName>Yun</LastName><ForeName>Dae-Jin</ForeName><Initials>DJ</Initials></Author><Author ValidYN="Y"><LastName>Noh</LastName><ForeName>Eun Woon</ForeName><Initials>EW</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Haeng-Soon</ForeName><Initials>HS</Initials></Author><Author ValidYN="Y"><LastName>Kwak</LastName><ForeName>Sang-Soo</ForeName><Initials>SS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biotechnol J</MedlineTA><NlmUniqueID>101201889</NlmUniqueID><ISSNLinking>1467-7644</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.4.-</RegistryNumber><NameOfSubstance UI="C121988">nucleoside diphosphate kinase 2</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.4.6</RegistryNumber><NameOfSubstance UI="D009701">Nucleoside-Diphosphate Kinase</NameOfSubstance></Chemical><Chemical><RegistryNumber>PLG39H7695</RegistryNumber><NameOfSubstance UI="D010269">Paraquat</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000222" MajorTopicYN="Y">Adaptation, Physiological</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009701" MajorTopicYN="N">Nucleoside-Diphosphate Kinase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="Y">Oxidative Stress</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010269" MajorTopicYN="N">Paraquat</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010788" MajorTopicYN="N">Photosynthesis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="Y">Stress, Physiological</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>7</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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