Over-expression of the triploid white poplar PtDrl01 gene in tobacco enhances resistance to tobacco mosaic virus.
Identifieur interne : 002F85 ( Main/Corpus ); précédent : 002F84; suivant : 002F86Over-expression of the triploid white poplar PtDrl01 gene in tobacco enhances resistance to tobacco mosaic virus.
Auteurs : H-Q Zheng ; Q. Zhang ; H-X Li ; S-Z Lin ; X-M An ; Z-Y ZhangSource :
- Plant biology (Stuttgart, Germany) [ 1438-8677 ] ; 2011.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Conserved Sequence (MeSH), Gene Expression Regulation, Plant (MeSH), Molecular Sequence Data (MeSH), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (metabolism), Sequence Alignment (MeSH), Tobacco (chemistry), Tobacco (genetics), Tobacco (metabolism), Tobacco (virology), Tobacco Mosaic Virus (physiology), Triploidy (MeSH).
- MESH :
- chemical , chemistry : Plant Proteins.
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Plant Proteins.
- chemistry : Tobacco.
- genetics : Tobacco.
- metabolism : Populus, Tobacco.
- physiology : Tobacco Mosaic Virus.
- virology : Tobacco.
- Amino Acid Sequence, Conserved Sequence, Gene Expression Regulation, Plant, Molecular Sequence Data, Sequence Alignment, Triploidy.
Abstract
A full-length cDNA, designated as the Populus tomentosa disease resistance-like 01 (PtDrl01) gene, was isolated from triploid white poplar [(Populus tomentosa × P. bolleana) × P. tomentosa]. The protein thought to be produced by the PtDrl01 gene contains a nuclear localisation sequence (NLS), a toll/interleukin-1 receptor (TIR) homologue region, a nucleotide binding site (NBS) and a leucine-rich repeat (LRR) domain. The protein also exhibits a considerable degree of homology to N-like resistance proteins. Real-time quantitative RT-PCR analysis revealed that expression of the PtDrl01 gene in triploid white poplar leaves could be induced by two defence signalling molecules: methyl jasmonate (MeJA) and salicylic acid (SA). Over-expression of the PtDrl01 gene in transgenic tobacco induced enhanced resistance to tobacco mosaic virus (TMV). Long-term resistance from the PtDrl01 gene to TMV infection was also observed in transgenic tobacco plants. Additionally, over-expression of the PtDrl01 gene resulted in transcriptional changes in genes expressing pathogenesis-related proteins in transgenic tobacco under non-stress conditions. These data strongly suggest that the PtDrl01 gene is involved in plant defence responses to pathogen infection.
DOI: 10.1111/j.1438-8677.2010.00327.x
PubMed: 21143735
Links to Exploration step
pubmed:21143735Le document en format XML
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<author><name sortKey="Zheng, H Q" sort="Zheng, H Q" uniqKey="Zheng H" first="H-Q" last="Zheng">H-Q Zheng</name>
<affiliation><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding of Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China.</nlm:affiliation>
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<author><name sortKey="Zhang, Q" sort="Zhang, Q" uniqKey="Zhang Q" first="Q" last="Zhang">Q. Zhang</name>
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<author><name sortKey="Li, H X" sort="Li, H X" uniqKey="Li H" first="H-X" last="Li">H-X Li</name>
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<author><name sortKey="Lin, S Z" sort="Lin, S Z" uniqKey="Lin S" first="S-Z" last="Lin">S-Z Lin</name>
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<author><name sortKey="An, X M" sort="An, X M" uniqKey="An X" first="X-M" last="An">X-M An</name>
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<author><name sortKey="Zhang, Z Y" sort="Zhang, Z Y" uniqKey="Zhang Z" first="Z-Y" last="Zhang">Z-Y Zhang</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Over-expression of the triploid white poplar PtDrl01 gene in tobacco enhances resistance to tobacco mosaic virus.</title>
<author><name sortKey="Zheng, H Q" sort="Zheng, H Q" uniqKey="Zheng H" first="H-Q" last="Zheng">H-Q Zheng</name>
<affiliation><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding of Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China.</nlm:affiliation>
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<author><name sortKey="Lin, S Z" sort="Lin, S Z" uniqKey="Lin S" first="S-Z" last="Lin">S-Z Lin</name>
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<author><name sortKey="An, X M" sort="An, X M" uniqKey="An X" first="X-M" last="An">X-M An</name>
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<author><name sortKey="Zhang, Z Y" sort="Zhang, Z Y" uniqKey="Zhang Z" first="Z-Y" last="Zhang">Z-Y Zhang</name>
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<series><title level="j">Plant biology (Stuttgart, Germany)</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Conserved Sequence (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Plant Proteins (chemistry)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Populus (metabolism)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Tobacco (chemistry)</term>
<term>Tobacco (genetics)</term>
<term>Tobacco (metabolism)</term>
<term>Tobacco (virology)</term>
<term>Tobacco Mosaic Virus (physiology)</term>
<term>Triploidy (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Tobacco</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Tobacco</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term>
<term>Tobacco</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Tobacco Mosaic Virus</term>
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<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Tobacco</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Conserved Sequence</term>
<term>Gene Expression Regulation, Plant</term>
<term>Molecular Sequence Data</term>
<term>Sequence Alignment</term>
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<front><div type="abstract" xml:lang="en">A full-length cDNA, designated as the Populus tomentosa disease resistance-like 01 (PtDrl01) gene, was isolated from triploid white poplar [(Populus tomentosa × P. bolleana) × P. tomentosa]. The protein thought to be produced by the PtDrl01 gene contains a nuclear localisation sequence (NLS), a toll/interleukin-1 receptor (TIR) homologue region, a nucleotide binding site (NBS) and a leucine-rich repeat (LRR) domain. The protein also exhibits a considerable degree of homology to N-like resistance proteins. Real-time quantitative RT-PCR analysis revealed that expression of the PtDrl01 gene in triploid white poplar leaves could be induced by two defence signalling molecules: methyl jasmonate (MeJA) and salicylic acid (SA). Over-expression of the PtDrl01 gene in transgenic tobacco induced enhanced resistance to tobacco mosaic virus (TMV). Long-term resistance from the PtDrl01 gene to TMV infection was also observed in transgenic tobacco plants. Additionally, over-expression of the PtDrl01 gene resulted in transcriptional changes in genes expressing pathogenesis-related proteins in transgenic tobacco under non-stress conditions. These data strongly suggest that the PtDrl01 gene is involved in plant defence responses to pathogen infection.</div>
</front>
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<Title>Plant biology (Stuttgart, Germany)</Title>
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<ArticleTitle>Over-expression of the triploid white poplar PtDrl01 gene in tobacco enhances resistance to tobacco mosaic virus.</ArticleTitle>
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<Abstract><AbstractText>A full-length cDNA, designated as the Populus tomentosa disease resistance-like 01 (PtDrl01) gene, was isolated from triploid white poplar [(Populus tomentosa × P. bolleana) × P. tomentosa]. The protein thought to be produced by the PtDrl01 gene contains a nuclear localisation sequence (NLS), a toll/interleukin-1 receptor (TIR) homologue region, a nucleotide binding site (NBS) and a leucine-rich repeat (LRR) domain. The protein also exhibits a considerable degree of homology to N-like resistance proteins. Real-time quantitative RT-PCR analysis revealed that expression of the PtDrl01 gene in triploid white poplar leaves could be induced by two defence signalling molecules: methyl jasmonate (MeJA) and salicylic acid (SA). Over-expression of the PtDrl01 gene in transgenic tobacco induced enhanced resistance to tobacco mosaic virus (TMV). Long-term resistance from the PtDrl01 gene to TMV infection was also observed in transgenic tobacco plants. Additionally, over-expression of the PtDrl01 gene resulted in transcriptional changes in genes expressing pathogenesis-related proteins in transgenic tobacco under non-stress conditions. These data strongly suggest that the PtDrl01 gene is involved in plant defence responses to pathogen infection.</AbstractText>
<CopyrightInformation>© 2010 German Botanical Society and The Royal Botanical Society of the Netherlands.</CopyrightInformation>
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