Transgenic poplars with reduced lignin show impaired xylem conductivity, growth efficiency and survival.
Identifieur interne : 002F30 ( Main/Corpus ); précédent : 002F29; suivant : 002F31Transgenic poplars with reduced lignin show impaired xylem conductivity, growth efficiency and survival.
Auteurs : Steven L. Voelker ; Barbara Lachenbruch ; Frederick C. Meinzer ; Peter Kitin ; Steven H. StraussSource :
- Plant, cell & environment [ 1365-3040 ] ; 2011.
English descriptors
- KwdEn :
- Biological Transport (MeSH), Biomass (MeSH), Biomechanical Phenomena (physiology), Coenzyme A Ligases (genetics), Coenzyme A Ligases (metabolism), Down-Regulation (MeSH), Lignin (metabolism), Plant Leaves (anatomy & histology), Plant Leaves (growth & development), Plant Leaves (physiology), Plant Stems (anatomy & histology), Plant Stems (enzymology), Plant Stems (genetics), Plant Stems (growth & development), Plant Transpiration (MeSH), Plants, Genetically Modified (enzymology), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (physiology), Populus (enzymology), Populus (genetics), Populus (growth & development), Populus (physiology), RNA, Antisense (genetics), Water (metabolism), Wood (metabolism), Xylem (anatomy & histology), Xylem (physiology).
- MESH :
- chemical , genetics : Coenzyme A Ligases, RNA, Antisense.
- chemical , metabolism : Coenzyme A Ligases, Lignin, Water.
- anatomy & histology : Plant Leaves, Plant Stems, Xylem.
- enzymology : Plant Stems, Plants, Genetically Modified, Populus.
- genetics : Plant Stems, Populus.
- growth & development : Plant Leaves, Plant Stems, Plants, Genetically Modified, Populus.
- metabolism : Wood.
- physiology : Biomechanical Phenomena, Plant Leaves, Plants, Genetically Modified, Populus, Xylem.
- Biological Transport, Biomass, Down-Regulation, Plant Transpiration.
Abstract
We studied xylem anatomy and hydraulic architecture in 14 transgenic insertion events and a control line of hybrid poplar (Populus spp.) that varied in lignin content. Transgenic events had different levels of down-regulation of two genes encoding 4-coumarate:coenzyme A ligase (4CL). Two-year-old trees were characterized after growing either as free-standing trees in the field or as supported by stakes in a greenhouse. In free-standing trees, a 20 to 40% reduction in lignin content was associated with increased xylem vulnerability to embolism, shoot dieback and mortality. In staked trees, the decreased biomechanical demands on the xylem was associated with increases in the leaf area to sapwood area ratio and wood specific conductivity (k(s)), and with decreased leaf-specific conductivity (k(l)). These shifts in hydraulic architecture suggest that the bending stresses perceived during growth can affect traits important for xylem water transport. Severe 4CL-downregulation resulted in the patchy formation of discoloured, brown wood with irregular vessels in which water transport was strongly impeded. These severely 4CL-downregulated trees had significantly lower growth efficiency (biomass/leaf area). These results underscore the necessity of adequate lignification for mechanical support of the stem, water transport, tree growth and survival.
DOI: 10.1111/j.1365-3040.2010.02270.x
PubMed: 21309794
Links to Exploration step
pubmed:21309794Le document en format XML
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Meinzer</name></author><author><name sortKey="Kitin, Peter" sort="Kitin, Peter" uniqKey="Kitin P" first="Peter" last="Kitin">Peter Kitin</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21309794</idno><idno type="pmid">21309794</idno><idno type="doi">10.1111/j.1365-3040.2010.02270.x</idno><idno type="wicri:Area/Main/Corpus">002F30</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002F30</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Transgenic poplars with reduced lignin show impaired xylem conductivity, growth efficiency and survival.</title><author><name sortKey="Voelker, Steven L" sort="Voelker, Steven L" uniqKey="Voelker S" first="Steven L" last="Voelker">Steven L. Voelker</name><affiliation><nlm:affiliation>Department of Wood Science and Engineering Department of Forest Ecosystems and Society, Oregon State University U.S.D.A. Forest Service, Forest Sciences Laboratory, 3200 Jefferson Way, Corvallis, OR 97330, USA. steve.voelker@oregonstate.edu</nlm:affiliation></affiliation></author><author><name sortKey="Lachenbruch, Barbara" sort="Lachenbruch, Barbara" uniqKey="Lachenbruch B" first="Barbara" last="Lachenbruch">Barbara Lachenbruch</name></author><author><name sortKey="Meinzer, Frederick C" sort="Meinzer, Frederick C" uniqKey="Meinzer F" first="Frederick C" last="Meinzer">Frederick C. Meinzer</name></author><author><name sortKey="Kitin, Peter" sort="Kitin, Peter" uniqKey="Kitin P" first="Peter" last="Kitin">Peter Kitin</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></analytic><series><title level="j">Plant, cell & environment</title><idno type="eISSN">1365-3040</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological Transport (MeSH)</term><term>Biomass (MeSH)</term><term>Biomechanical Phenomena (physiology)</term><term>Coenzyme A Ligases (genetics)</term><term>Coenzyme A Ligases (metabolism)</term><term>Down-Regulation (MeSH)</term><term>Lignin (metabolism)</term><term>Plant Leaves (anatomy & histology)</term><term>Plant Leaves (growth & development)</term><term>Plant Leaves (physiology)</term><term>Plant Stems (anatomy & histology)</term><term>Plant Stems (enzymology)</term><term>Plant Stems (genetics)</term><term>Plant Stems (growth & development)</term><term>Plant Transpiration (MeSH)</term><term>Plants, Genetically Modified (enzymology)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (physiology)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Populus (physiology)</term><term>RNA, Antisense (genetics)</term><term>Water (metabolism)</term><term>Wood (metabolism)</term><term>Xylem (anatomy & histology)</term><term>Xylem (physiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Coenzyme A Ligases</term><term>RNA, Antisense</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Coenzyme A Ligases</term><term>Lignin</term><term>Water</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Plant Leaves</term><term>Plant Stems</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Plant Stems</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Stems</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Leaves</term><term>Plant Stems</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Wood</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Biomechanical Phenomena</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Transport</term><term>Biomass</term><term>Down-Regulation</term><term>Plant Transpiration</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We studied xylem anatomy and hydraulic architecture in 14 transgenic insertion events and a control line of hybrid poplar (Populus spp.) that varied in lignin content. Transgenic events had different levels of down-regulation of two genes encoding 4-coumarate:coenzyme A ligase (4CL). Two-year-old trees were characterized after growing either as free-standing trees in the field or as supported by stakes in a greenhouse. In free-standing trees, a 20 to 40% reduction in lignin content was associated with increased xylem vulnerability to embolism, shoot dieback and mortality. In staked trees, the decreased biomechanical demands on the xylem was associated with increases in the leaf area to sapwood area ratio and wood specific conductivity (k(s)), and with decreased leaf-specific conductivity (k(l)). These shifts in hydraulic architecture suggest that the bending stresses perceived during growth can affect traits important for xylem water transport. Severe 4CL-downregulation resulted in the patchy formation of discoloured, brown wood with irregular vessels in which water transport was strongly impeded. These severely 4CL-downregulated trees had significantly lower growth efficiency (biomass/leaf area). These results underscore the necessity of adequate lignification for mechanical support of the stem, water transport, tree growth and survival.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21309794</PMID><DateCompleted><Year>2011</Year><Month>06</Month><Day>28</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-3040</ISSN><JournalIssue CitedMedium="Internet"><Volume>34</Volume><Issue>4</Issue><PubDate><Year>2011</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Plant, cell & environment</Title><ISOAbbreviation>Plant Cell Environ</ISOAbbreviation></Journal><ArticleTitle>Transgenic poplars with reduced lignin show impaired xylem conductivity, growth efficiency and survival.</ArticleTitle><Pagination><MedlinePgn>655-68</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1365-3040.2010.02270.x</ELocationID><Abstract><AbstractText>We studied xylem anatomy and hydraulic architecture in 14 transgenic insertion events and a control line of hybrid poplar (Populus spp.) that varied in lignin content. Transgenic events had different levels of down-regulation of two genes encoding 4-coumarate:coenzyme A ligase (4CL). Two-year-old trees were characterized after growing either as free-standing trees in the field or as supported by stakes in a greenhouse. In free-standing trees, a 20 to 40% reduction in lignin content was associated with increased xylem vulnerability to embolism, shoot dieback and mortality. In staked trees, the decreased biomechanical demands on the xylem was associated with increases in the leaf area to sapwood area ratio and wood specific conductivity (k(s)), and with decreased leaf-specific conductivity (k(l)). These shifts in hydraulic architecture suggest that the bending stresses perceived during growth can affect traits important for xylem water transport. Severe 4CL-downregulation resulted in the patchy formation of discoloured, brown wood with irregular vessels in which water transport was strongly impeded. These severely 4CL-downregulated trees had significantly lower growth efficiency (biomass/leaf area). These results underscore the necessity of adequate lignification for mechanical support of the stem, water transport, tree growth and survival.</AbstractText><CopyrightInformation>© 2011 Blackwell Publishing Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Voelker</LastName><ForeName>Steven L</ForeName><Initials>SL</Initials><AffiliationInfo><Affiliation>Department of Wood Science and Engineering Department of Forest Ecosystems and Society, Oregon State University U.S.D.A. Forest Service, Forest Sciences Laboratory, 3200 Jefferson Way, Corvallis, OR 97330, USA. steve.voelker@oregonstate.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lachenbruch</LastName><ForeName>Barbara</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Meinzer</LastName><ForeName>Frederick C</ForeName><Initials>FC</Initials></Author><Author ValidYN="Y"><LastName>Kitin</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Strauss</LastName><ForeName>Steven H</ForeName><Initials>SH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>02</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Cell Environ</MedlineTA><NlmUniqueID>9309004</NlmUniqueID><ISSNLinking>0140-7791</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016372">RNA, Antisense</NameOfSubstance></Chemical><Chemical><RegistryNumber>059QF0KO0R</RegistryNumber><NameOfSubstance UI="D014867">Water</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 6.2.1.-</RegistryNumber><NameOfSubstance UI="D003066">Coenzyme A Ligases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001692" MajorTopicYN="N">Biological Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001696" MajorTopicYN="N">Biomechanical Phenomena</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003066" MajorTopicYN="N">Coenzyme A Ligases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015536" MajorTopicYN="N">Down-Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018526" MajorTopicYN="N">Plant Transpiration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016372" MajorTopicYN="N">RNA, Antisense</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014867" MajorTopicYN="N">Water</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>2</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>2</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>6</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21309794</ArticleId><ArticleId IdType="doi">10.1111/j.1365-3040.2010.02270.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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