Enhanced arsenic tolerance of transgenic eastern cottonwood plants expressing gamma-glutamylcysteine synthetase.
Identifieur interne : 002C72 ( Main/Corpus ); précédent : 002C71; suivant : 002C73Enhanced arsenic tolerance of transgenic eastern cottonwood plants expressing gamma-glutamylcysteine synthetase.
Auteurs : Melissa S. Leblanc ; Amparo Lima ; Paul Montello ; Tehryoung Kim ; Richard B. Meagher ; Scott MerkleSource :
- International journal of phytoremediation [ 1522-6514 ] ; 2011.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Arabidopsis (genetics), Arsenic (analysis), Arsenic (metabolism), Arsenic (toxicity), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Biodegradation, Environmental (MeSH), Escherichia coli (enzymology), Escherichia coli (genetics), Gene Expression Regulation, Enzymologic (MeSH), Gene Expression Regulation, Plant (MeSH), Glutamate-Cysteine Ligase (genetics), Glutamate-Cysteine Ligase (metabolism), Plant Roots (chemistry), Plant Roots (drug effects), Plant Roots (growth & development), Plant Shoots (chemistry), Plant Shoots (drug effects), Plant Shoots (growth & development), Plants, Genetically Modified (MeSH), Populus (drug effects), Populus (enzymology), Populus (genetics), Populus (growth & development), Promoter Regions, Genetic (genetics), Sulfhydryl Compounds (metabolism), Tissue Culture Techniques (MeSH).
- MESH :
- chemical , analysis : Arsenic.
- chemistry : Plant Roots, Plant Shoots.
- drug effects : Plant Roots, Plant Shoots, Populus.
- enzymology : Escherichia coli, Populus.
- genetics : Agrobacterium tumefaciens, Arabidopsis, Bacterial Proteins, Escherichia coli, Glutamate-Cysteine Ligase, Populus, Promoter Regions, Genetic.
- growth & development : Plant Roots, Plant Shoots, Populus.
- chemical , metabolism : Arsenic, Bacterial Proteins, Glutamate-Cysteine Ligase, Sulfhydryl Compounds.
- chemical , toxicity : Arsenic.
- Biodegradation, Environmental, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Plants, Genetically Modified, Tissue Culture Techniques.
Abstract
Arsenic is a metalloid that occurs naturally at parts per million (ppm) levels in the earth's crust. Natural and human activities have contributed to arsenic mobilization and increased concentration in the environment, such that World Health Organization guidelines for arsenic levels in drinking water are exceeded at many locations, worldwide. This translates into an increased risk of arsenic-related illnesses for millions of people. Recent studies demonstrate that increasing thiol-sinks in transgenic plants by overexpressing the bacterial gamma-glutamylcysteine synthetase (ECS) gene results in a higher tolerance and accumulation of metals and metalloids such as cadmium, mercury, and arsenic. We used Agrobacterium-mediated transformation to genetically engineer eastern cottonwood with a bacterial ECS gene. Eastern cottonwood plants expressing ECS had elevated thiol group levels, consistent with increased ECS activity. In addition, these ECS-expressing plants had enhanced growth on levels of arsenate toxic to control plants in vitro. Furthermore, roots of ECS-expressing plants accumulated significantly more arsenic than control roots (approximately twice as much), while shoots accumulated significantly less arsenic than control shoots (approximately two-thirds as much). We discuss potential mechanisms for shifting the balance of plant arsenic distribution from root accumulation to shoot accumulation, as it pertains to arsenic phytoremediation.
DOI: 10.1080/15226514.2010.499917
PubMed: 21972493
Links to Exploration step
pubmed:21972493Le document en format XML
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<author><name sortKey="Lima, Amparo" sort="Lima, Amparo" uniqKey="Lima A" first="Amparo" last="Lima">Amparo Lima</name>
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<author><name sortKey="Montello, Paul" sort="Montello, Paul" uniqKey="Montello P" first="Paul" last="Montello">Paul Montello</name>
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<author><name sortKey="Kim, Tehryoung" sort="Kim, Tehryoung" uniqKey="Kim T" first="Tehryoung" last="Kim">Tehryoung Kim</name>
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<author><name sortKey="Meagher, Richard B" sort="Meagher, Richard B" uniqKey="Meagher R" first="Richard B" last="Meagher">Richard B. Meagher</name>
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<term>Arsenic (metabolism)</term>
<term>Arsenic (toxicity)</term>
<term>Bacterial Proteins (genetics)</term>
<term>Bacterial Proteins (metabolism)</term>
<term>Biodegradation, Environmental (MeSH)</term>
<term>Escherichia coli (enzymology)</term>
<term>Escherichia coli (genetics)</term>
<term>Gene Expression Regulation, Enzymologic (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Glutamate-Cysteine Ligase (genetics)</term>
<term>Glutamate-Cysteine Ligase (metabolism)</term>
<term>Plant Roots (chemistry)</term>
<term>Plant Roots (drug effects)</term>
<term>Plant Roots (growth & development)</term>
<term>Plant Shoots (chemistry)</term>
<term>Plant Shoots (drug effects)</term>
<term>Plant Shoots (growth & development)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Populus (drug effects)</term>
<term>Populus (enzymology)</term>
<term>Populus (genetics)</term>
<term>Populus (growth & development)</term>
<term>Promoter Regions, Genetic (genetics)</term>
<term>Sulfhydryl Compounds (metabolism)</term>
<term>Tissue Culture Techniques (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Arsenic</term>
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<term>Plant Shoots</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Escherichia coli</term>
<term>Populus</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Arabidopsis</term>
<term>Bacterial Proteins</term>
<term>Escherichia coli</term>
<term>Glutamate-Cysteine Ligase</term>
<term>Populus</term>
<term>Promoter Regions, Genetic</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Arsenic</term>
<term>Bacterial Proteins</term>
<term>Glutamate-Cysteine Ligase</term>
<term>Sulfhydryl Compounds</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Arsenic</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term>
<term>Gene Expression Regulation, Enzymologic</term>
<term>Gene Expression Regulation, Plant</term>
<term>Plants, Genetically Modified</term>
<term>Tissue Culture Techniques</term>
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<front><div type="abstract" xml:lang="en">Arsenic is a metalloid that occurs naturally at parts per million (ppm) levels in the earth's crust. Natural and human activities have contributed to arsenic mobilization and increased concentration in the environment, such that World Health Organization guidelines for arsenic levels in drinking water are exceeded at many locations, worldwide. This translates into an increased risk of arsenic-related illnesses for millions of people. Recent studies demonstrate that increasing thiol-sinks in transgenic plants by overexpressing the bacterial gamma-glutamylcysteine synthetase (ECS) gene results in a higher tolerance and accumulation of metals and metalloids such as cadmium, mercury, and arsenic. We used Agrobacterium-mediated transformation to genetically engineer eastern cottonwood with a bacterial ECS gene. Eastern cottonwood plants expressing ECS had elevated thiol group levels, consistent with increased ECS activity. In addition, these ECS-expressing plants had enhanced growth on levels of arsenate toxic to control plants in vitro. Furthermore, roots of ECS-expressing plants accumulated significantly more arsenic than control roots (approximately twice as much), while shoots accumulated significantly less arsenic than control shoots (approximately two-thirds as much). We discuss potential mechanisms for shifting the balance of plant arsenic distribution from root accumulation to shoot accumulation, as it pertains to arsenic phytoremediation.</div>
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<Abstract><AbstractText>Arsenic is a metalloid that occurs naturally at parts per million (ppm) levels in the earth's crust. Natural and human activities have contributed to arsenic mobilization and increased concentration in the environment, such that World Health Organization guidelines for arsenic levels in drinking water are exceeded at many locations, worldwide. This translates into an increased risk of arsenic-related illnesses for millions of people. Recent studies demonstrate that increasing thiol-sinks in transgenic plants by overexpressing the bacterial gamma-glutamylcysteine synthetase (ECS) gene results in a higher tolerance and accumulation of metals and metalloids such as cadmium, mercury, and arsenic. We used Agrobacterium-mediated transformation to genetically engineer eastern cottonwood with a bacterial ECS gene. Eastern cottonwood plants expressing ECS had elevated thiol group levels, consistent with increased ECS activity. In addition, these ECS-expressing plants had enhanced growth on levels of arsenate toxic to control plants in vitro. Furthermore, roots of ECS-expressing plants accumulated significantly more arsenic than control roots (approximately twice as much), while shoots accumulated significantly less arsenic than control shoots (approximately two-thirds as much). We discuss potential mechanisms for shifting the balance of plant arsenic distribution from root accumulation to shoot accumulation, as it pertains to arsenic phytoremediation.</AbstractText>
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