Bacillus thuringiensis protein transfer between rootstock and scion of grafted poplar.
Identifieur interne : 002B22 ( Main/Corpus ); précédent : 002B21; suivant : 002B23Bacillus thuringiensis protein transfer between rootstock and scion of grafted poplar.
Auteurs : L. Wang ; M. Yang ; A. Akinnagbe ; H. Liang ; J. Wang ; D. EwaldSource :
- Plant biology (Stuttgart, Germany) [ 1438-8677 ] ; 2012.
English descriptors
- KwdEn :
- Animals (MeSH), Bacillus thuringiensis (metabolism), Bacterial Proteins (metabolism), Endotoxins (metabolism), Hemolysin Proteins (metabolism), Larva (growth & development), Lepidoptera (physiology), Plant Leaves (metabolism), Plants, Genetically Modified (MeSH), Populus (metabolism), Protein Transport (MeSH).
- MESH :
- chemical , metabolism : Bacterial Proteins, Endotoxins, Hemolysin Proteins.
- growth & development : Larva.
- metabolism : Bacillus thuringiensis, Plant Leaves, Populus.
- physiology : Lepidoptera.
- Animals, Plants, Genetically Modified, Protein Transport.
Abstract
Bacillus thuringiensis (Bt) Cry1Ac protein is a toxin against different leaf-eating lepidopteran insects that attack poplar trees. In the present study, the mode of migration of the Bt-Cry1Ac protein within poplar grafts was investigated. Grafting was done using Pb29 (transgenic poplar 741 with cry1Ac genes), CC71 (transgenic poplar 741 with cry3A genes), non-transgenic poplar 741 and non-transgenic Populus tomentosa, either as scion or as rootstock. In order to detect migration of Bt-Cry1Ac protein from one portion of the graft union to different tissues in the grafted plant, ELISA analysis was employed to assess the content of Bt-Cry1Ac protein in the phloem, xylem, pith and leaves of the grafted poplar. To further verify migration of Bt-Cry1Ac protein, Clostera anachoreta larvae, which are susceptible to Bt-Cry1Ac protein, were fed leaves from the control graft (i.e., graft portion that originally did not contain Bt-Cry1Ac protein). The results showed that Bt-Cry1Ac protein was transported between rootstock and scion mainly through the phloem. Migration of Bt-Cry1Ac protein in the grafted union was also evidenced in that the leaves of the control graft did have a lethal effect on C. anachoreta larvae in laboratory feeding experiments.
DOI: 10.1111/j.1438-8677.2011.00555.x
PubMed: 22372666
Links to Exploration step
pubmed:22372666Le document en format XML
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<author><name sortKey="Wang, L" sort="Wang, L" uniqKey="Wang L" first="L" last="Wang">L. Wang</name>
<affiliation><nlm:affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China College of Horticulture, Hebei North University, Zhangjiakou, China Department of Forestry & Wood Technology, Federal University of Technology, Akure, Nigeria Institute of Forest Genetics, Johann Heinrich von Thuenen Institute Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.</nlm:affiliation>
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<author><name sortKey="Yang, M" sort="Yang, M" uniqKey="Yang M" first="M" last="Yang">M. Yang</name>
<affiliation><nlm:affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China College of Horticulture, Hebei North University, Zhangjiakou, China Department of Forestry & Wood Technology, Federal University of Technology, Akure, Nigeria Institute of Forest Genetics, Johann Heinrich von Thuenen Institute Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.</nlm:affiliation>
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<author><name sortKey="Akinnagbe, A" sort="Akinnagbe, A" uniqKey="Akinnagbe A" first="A" last="Akinnagbe">A. Akinnagbe</name>
<affiliation><nlm:affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China College of Horticulture, Hebei North University, Zhangjiakou, China Department of Forestry & Wood Technology, Federal University of Technology, Akure, Nigeria Institute of Forest Genetics, Johann Heinrich von Thuenen Institute Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.</nlm:affiliation>
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<author><name sortKey="Liang, H" sort="Liang, H" uniqKey="Liang H" first="H" last="Liang">H. Liang</name>
<affiliation><nlm:affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China College of Horticulture, Hebei North University, Zhangjiakou, China Department of Forestry & Wood Technology, Federal University of Technology, Akure, Nigeria Institute of Forest Genetics, Johann Heinrich von Thuenen Institute Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.</nlm:affiliation>
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<author><name sortKey="Wang, J" sort="Wang, J" uniqKey="Wang J" first="J" last="Wang">J. Wang</name>
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<author><name sortKey="Ewald, D" sort="Ewald, D" uniqKey="Ewald D" first="D" last="Ewald">D. Ewald</name>
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<author><name sortKey="Wang, L" sort="Wang, L" uniqKey="Wang L" first="L" last="Wang">L. Wang</name>
<affiliation><nlm:affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China College of Horticulture, Hebei North University, Zhangjiakou, China Department of Forestry & Wood Technology, Federal University of Technology, Akure, Nigeria Institute of Forest Genetics, Johann Heinrich von Thuenen Institute Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.</nlm:affiliation>
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<author><name sortKey="Yang, M" sort="Yang, M" uniqKey="Yang M" first="M" last="Yang">M. Yang</name>
<affiliation><nlm:affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China College of Horticulture, Hebei North University, Zhangjiakou, China Department of Forestry & Wood Technology, Federal University of Technology, Akure, Nigeria Institute of Forest Genetics, Johann Heinrich von Thuenen Institute Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.</nlm:affiliation>
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<author><name sortKey="Akinnagbe, A" sort="Akinnagbe, A" uniqKey="Akinnagbe A" first="A" last="Akinnagbe">A. Akinnagbe</name>
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<author><name sortKey="Liang, H" sort="Liang, H" uniqKey="Liang H" first="H" last="Liang">H. Liang</name>
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<author><name sortKey="Wang, J" sort="Wang, J" uniqKey="Wang J" first="J" last="Wang">J. Wang</name>
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<series><title level="j">Plant biology (Stuttgart, Germany)</title>
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<term>Bacillus thuringiensis (metabolism)</term>
<term>Bacterial Proteins (metabolism)</term>
<term>Endotoxins (metabolism)</term>
<term>Hemolysin Proteins (metabolism)</term>
<term>Larva (growth & development)</term>
<term>Lepidoptera (physiology)</term>
<term>Plant Leaves (metabolism)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Populus (metabolism)</term>
<term>Protein Transport (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term>
<term>Endotoxins</term>
<term>Hemolysin Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Larva</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacillus thuringiensis</term>
<term>Plant Leaves</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Lepidoptera</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Plants, Genetically Modified</term>
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<front><div type="abstract" xml:lang="en">Bacillus thuringiensis (Bt) Cry1Ac protein is a toxin against different leaf-eating lepidopteran insects that attack poplar trees. In the present study, the mode of migration of the Bt-Cry1Ac protein within poplar grafts was investigated. Grafting was done using Pb29 (transgenic poplar 741 with cry1Ac genes), CC71 (transgenic poplar 741 with cry3A genes), non-transgenic poplar 741 and non-transgenic Populus tomentosa, either as scion or as rootstock. In order to detect migration of Bt-Cry1Ac protein from one portion of the graft union to different tissues in the grafted plant, ELISA analysis was employed to assess the content of Bt-Cry1Ac protein in the phloem, xylem, pith and leaves of the grafted poplar. To further verify migration of Bt-Cry1Ac protein, Clostera anachoreta larvae, which are susceptible to Bt-Cry1Ac protein, were fed leaves from the control graft (i.e., graft portion that originally did not contain Bt-Cry1Ac protein). The results showed that Bt-Cry1Ac protein was transported between rootstock and scion mainly through the phloem. Migration of Bt-Cry1Ac protein in the grafted union was also evidenced in that the leaves of the control graft did have a lethal effect on C. anachoreta larvae in laboratory feeding experiments. </div>
</front>
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<Title>Plant biology (Stuttgart, Germany)</Title>
<ISOAbbreviation>Plant Biol (Stuttg)</ISOAbbreviation>
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<ArticleTitle>Bacillus thuringiensis protein transfer between rootstock and scion of grafted poplar.</ArticleTitle>
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<Abstract><AbstractText>Bacillus thuringiensis (Bt) Cry1Ac protein is a toxin against different leaf-eating lepidopteran insects that attack poplar trees. In the present study, the mode of migration of the Bt-Cry1Ac protein within poplar grafts was investigated. Grafting was done using Pb29 (transgenic poplar 741 with cry1Ac genes), CC71 (transgenic poplar 741 with cry3A genes), non-transgenic poplar 741 and non-transgenic Populus tomentosa, either as scion or as rootstock. In order to detect migration of Bt-Cry1Ac protein from one portion of the graft union to different tissues in the grafted plant, ELISA analysis was employed to assess the content of Bt-Cry1Ac protein in the phloem, xylem, pith and leaves of the grafted poplar. To further verify migration of Bt-Cry1Ac protein, Clostera anachoreta larvae, which are susceptible to Bt-Cry1Ac protein, were fed leaves from the control graft (i.e., graft portion that originally did not contain Bt-Cry1Ac protein). The results showed that Bt-Cry1Ac protein was transported between rootstock and scion mainly through the phloem. Migration of Bt-Cry1Ac protein in the grafted union was also evidenced in that the leaves of the control graft did have a lethal effect on C. anachoreta larvae in laboratory feeding experiments. </AbstractText>
<CopyrightInformation>© 2012 German Botanical Society and The Royal Botanical Society of the Netherlands.</CopyrightInformation>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>L</ForeName>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Clostera anachoreta</Keyword>
<Keyword MajorTopicYN="N">Cry1Ac protein</Keyword>
<Keyword MajorTopicYN="N">Populus</Keyword>
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