Two poplar cellulose synthase-like D genes, PdCSLD5 and PdCSLD6, are functionally conserved with Arabidopsis CSLD3.
Identifieur interne : 002568 ( Main/Corpus ); précédent : 002567; suivant : 002569Two poplar cellulose synthase-like D genes, PdCSLD5 and PdCSLD6, are functionally conserved with Arabidopsis CSLD3.
Auteurs : Guang Qi ; Ruibo Hu ; Li Yu ; Guohua Chai ; Yingping Cao ; Ran Zuo ; Yingzhen Kong ; Gongke ZhouSource :
- Journal of plant physiology [ 1618-1328 ] ; 2013.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Arabidopsis (genetics), Arabidopsis (growth & development), Arabidopsis (metabolism), Arabidopsis Proteins (chemistry), Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), Carbohydrate Metabolism (MeSH), Cell Wall (metabolism), Cell Wall (ultrastructure), Cellulose (metabolism), Gene Expression Regulation, Plant (MeSH), Glucosyltransferases (chemistry), Glucosyltransferases (genetics), Glucosyltransferases (metabolism), Microscopy, Confocal (MeSH), Microscopy, Fluorescence (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Roots (cytology), Plant Roots (growth & development), Plant Roots (metabolism), Populus (genetics), Populus (growth & development), Populus (metabolism), Real-Time Polymerase Chain Reaction (MeSH), Sequence Alignment (MeSH).
- MESH :
- chemical , chemistry : Arabidopsis Proteins, Glucosyltransferases, Plant Proteins.
- cytology : Plant Roots.
- genetics : Arabidopsis, Arabidopsis Proteins, Glucosyltransferases, Plant Proteins, Populus.
- growth & development : Arabidopsis, Plant Roots, Populus.
- metabolism : Arabidopsis, Arabidopsis Proteins, Cell Wall, Cellulose, Glucosyltransferases, Plant Proteins, Plant Roots, Populus.
- ultrastructure : Cell Wall.
- Amino Acid Sequence, Carbohydrate Metabolism, Gene Expression Regulation, Plant, Microscopy, Confocal, Microscopy, Fluorescence, Molecular Sequence Data, Phylogeny, Real-Time Polymerase Chain Reaction, Sequence Alignment.
Abstract
Root hairs are tip-growing long tubular outgrowths of specialized epidermal cells, and are important for nutrient and water uptake and interaction with the soil microflora. Here we characterized two poplar cellulose synthase-like D (CSLD) genes, PdCSLD5 and PdCSLD6, the most probable orthologs to the Arabidopsis AtCSLD3/KOJAK gene. Both PdCSLD5 and PdCSLD6 are strongly expressed in roots, including in the root hairs. Subcellular localization experiments showed that these two proteins are located not only in the polarized plasma membrane of root hair tips, but also in Golgi apparatus of the root hair and non-hair-forming cells. Overexpression of these two poplar genes in the atcsld3 mutant was able to rescue most of the defects caused by disruption of AtCSLD3, including root hair morphological changes, altered cell wall monosaccharide composition, increased non-crystalline β-1,4-glucan and decreased crystalline cellulose contents. Taken together, our results provide evidence indicating that PdCSLD5 and PdCSLD6 are functionally conserved with AtCSLD3 and support a role for PdCSLD5 and PdCSL6 specifically in crystalline cellulose production in poplar root hair tips. The results presented here also suggest that at least part of the mechanism of root hair formation is conserved between herbaceous and woody plants.
DOI: 10.1016/j.jplph.2013.04.014
PubMed: 23746994
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Two poplar cellulose synthase-like D genes, PdCSLD5 and PdCSLD6, are functionally conserved with Arabidopsis CSLD3.</title><author><name sortKey="Qi, Guang" sort="Qi, Guang" uniqKey="Qi G" first="Guang" last="Qi">Guang Qi</name><affiliation><nlm:affiliation>Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Hu, Ruibo" sort="Hu, Ruibo" uniqKey="Hu R" first="Ruibo" last="Hu">Ruibo Hu</name></author><author><name sortKey="Yu, Li" sort="Yu, Li" uniqKey="Yu L" first="Li" last="Yu">Li Yu</name></author><author><name sortKey="Chai, Guohua" sort="Chai, Guohua" uniqKey="Chai G" first="Guohua" last="Chai">Guohua Chai</name></author><author><name sortKey="Cao, Yingping" sort="Cao, Yingping" uniqKey="Cao Y" first="Yingping" last="Cao">Yingping Cao</name></author><author><name sortKey="Zuo, Ran" sort="Zuo, Ran" uniqKey="Zuo R" first="Ran" last="Zuo">Ran Zuo</name></author><author><name sortKey="Kong, Yingzhen" sort="Kong, Yingzhen" uniqKey="Kong Y" first="Yingzhen" last="Kong">Yingzhen Kong</name></author><author><name sortKey="Zhou, Gongke" sort="Zhou, Gongke" uniqKey="Zhou G" first="Gongke" last="Zhou">Gongke Zhou</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23746994</idno><idno type="pmid">23746994</idno><idno type="doi">10.1016/j.jplph.2013.04.014</idno><idno type="wicri:Area/Main/Corpus">002568</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002568</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Two poplar cellulose synthase-like D genes, PdCSLD5 and PdCSLD6, are functionally conserved with Arabidopsis CSLD3.</title><author><name sortKey="Qi, Guang" sort="Qi, Guang" uniqKey="Qi G" first="Guang" last="Qi">Guang Qi</name><affiliation><nlm:affiliation>Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Hu, Ruibo" sort="Hu, Ruibo" uniqKey="Hu R" first="Ruibo" last="Hu">Ruibo Hu</name></author><author><name sortKey="Yu, Li" sort="Yu, Li" uniqKey="Yu L" first="Li" last="Yu">Li Yu</name></author><author><name sortKey="Chai, Guohua" sort="Chai, Guohua" uniqKey="Chai G" first="Guohua" last="Chai">Guohua Chai</name></author><author><name sortKey="Cao, Yingping" sort="Cao, Yingping" uniqKey="Cao Y" first="Yingping" last="Cao">Yingping Cao</name></author><author><name sortKey="Zuo, Ran" sort="Zuo, Ran" uniqKey="Zuo R" first="Ran" last="Zuo">Ran Zuo</name></author><author><name sortKey="Kong, Yingzhen" sort="Kong, Yingzhen" uniqKey="Kong Y" first="Yingzhen" last="Kong">Yingzhen Kong</name></author><author><name sortKey="Zhou, Gongke" sort="Zhou, Gongke" uniqKey="Zhou G" first="Gongke" last="Zhou">Gongke Zhou</name></author></analytic><series><title level="j">Journal of plant physiology</title><idno type="eISSN">1618-1328</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Arabidopsis (genetics)</term><term>Arabidopsis (growth & development)</term><term>Arabidopsis (metabolism)</term><term>Arabidopsis Proteins (chemistry)</term><term>Arabidopsis Proteins (genetics)</term><term>Arabidopsis Proteins (metabolism)</term><term>Carbohydrate Metabolism (MeSH)</term><term>Cell Wall (metabolism)</term><term>Cell Wall (ultrastructure)</term><term>Cellulose (metabolism)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Glucosyltransferases (chemistry)</term><term>Glucosyltransferases (genetics)</term><term>Glucosyltransferases (metabolism)</term><term>Microscopy, Confocal (MeSH)</term><term>Microscopy, Fluorescence (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plant Roots (cytology)</term><term>Plant Roots (growth & development)</term><term>Plant Roots (metabolism)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Populus (metabolism)</term><term>Real-Time Polymerase Chain Reaction (MeSH)</term><term>Sequence Alignment (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Arabidopsis Proteins</term><term>Glucosyltransferases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Arabidopsis Proteins</term><term>Glucosyltransferases</term><term>Plant Proteins</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Arabidopsis</term><term>Plant Roots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>Arabidopsis Proteins</term><term>Cell Wall</term><term>Cellulose</term><term>Glucosyltransferases</term><term>Plant Proteins</term><term>Plant Roots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Cell Wall</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Carbohydrate Metabolism</term><term>Gene Expression Regulation, Plant</term><term>Microscopy, Confocal</term><term>Microscopy, Fluorescence</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Real-Time Polymerase Chain Reaction</term><term>Sequence Alignment</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Root hairs are tip-growing long tubular outgrowths of specialized epidermal cells, and are important for nutrient and water uptake and interaction with the soil microflora. Here we characterized two poplar cellulose synthase-like D (CSLD) genes, PdCSLD5 and PdCSLD6, the most probable orthologs to the Arabidopsis AtCSLD3/KOJAK gene. Both PdCSLD5 and PdCSLD6 are strongly expressed in roots, including in the root hairs. Subcellular localization experiments showed that these two proteins are located not only in the polarized plasma membrane of root hair tips, but also in Golgi apparatus of the root hair and non-hair-forming cells. Overexpression of these two poplar genes in the atcsld3 mutant was able to rescue most of the defects caused by disruption of AtCSLD3, including root hair morphological changes, altered cell wall monosaccharide composition, increased non-crystalline β-1,4-glucan and decreased crystalline cellulose contents. Taken together, our results provide evidence indicating that PdCSLD5 and PdCSLD6 are functionally conserved with AtCSLD3 and support a role for PdCSLD5 and PdCSL6 specifically in crystalline cellulose production in poplar root hair tips. The results presented here also suggest that at least part of the mechanism of root hair formation is conserved between herbaceous and woody plants. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23746994</PMID><DateCompleted><Year>2014</Year><Month>03</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-1328</ISSN><JournalIssue CitedMedium="Internet"><Volume>170</Volume><Issue>14</Issue><PubDate><Year>2013</Year><Month>Sep</Month><Day>15</Day></PubDate></JournalIssue><Title>Journal of plant physiology</Title><ISOAbbreviation>J Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Two poplar cellulose synthase-like D genes, PdCSLD5 and PdCSLD6, are functionally conserved with Arabidopsis CSLD3.</ArticleTitle><Pagination><MedlinePgn>1267-76</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jplph.2013.04.014</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0176-1617(13)00192-2</ELocationID><Abstract><AbstractText>Root hairs are tip-growing long tubular outgrowths of specialized epidermal cells, and are important for nutrient and water uptake and interaction with the soil microflora. Here we characterized two poplar cellulose synthase-like D (CSLD) genes, PdCSLD5 and PdCSLD6, the most probable orthologs to the Arabidopsis AtCSLD3/KOJAK gene. Both PdCSLD5 and PdCSLD6 are strongly expressed in roots, including in the root hairs. Subcellular localization experiments showed that these two proteins are located not only in the polarized plasma membrane of root hair tips, but also in Golgi apparatus of the root hair and non-hair-forming cells. Overexpression of these two poplar genes in the atcsld3 mutant was able to rescue most of the defects caused by disruption of AtCSLD3, including root hair morphological changes, altered cell wall monosaccharide composition, increased non-crystalline β-1,4-glucan and decreased crystalline cellulose contents. Taken together, our results provide evidence indicating that PdCSLD5 and PdCSLD6 are functionally conserved with AtCSLD3 and support a role for PdCSLD5 and PdCSL6 specifically in crystalline cellulose production in poplar root hair tips. The results presented here also suggest that at least part of the mechanism of root hair formation is conserved between herbaceous and woody plants. </AbstractText><CopyrightInformation>Copyright © 2013 Elsevier GmbH. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Qi</LastName><ForeName>Guang</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Ruibo</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Li</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Chai</LastName><ForeName>Guohua</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Yingping</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Zuo</LastName><ForeName>Ran</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Kong</LastName><ForeName>Yingzhen</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Gongke</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>06</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Plant Physiol</MedlineTA><NlmUniqueID>9882059</NlmUniqueID><ISSNLinking>0176-1617</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029681">Arabidopsis Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="D005964">Glucosyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="C478648">cellulose synthase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029681" MajorTopicYN="N">Arabidopsis Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="N">Carbohydrate Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005964" MajorTopicYN="N">Glucosyltransferases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008856" MajorTopicYN="N">Microscopy, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">CESA-like</Keyword><Keyword MajorTopicYN="N">CSL</Keyword><Keyword MajorTopicYN="N">Cellulose synthase-like D</Keyword><Keyword MajorTopicYN="N">Crystalline cellulose</Keyword><Keyword MajorTopicYN="N">GFP</Keyword><Keyword MajorTopicYN="N">GUS</Keyword><Keyword MajorTopicYN="N">Poplar</Keyword><Keyword MajorTopicYN="N">Root hair</Keyword><Keyword MajorTopicYN="N">green fluorescent protein</Keyword><Keyword MajorTopicYN="N">qRT-PCR</Keyword><Keyword MajorTopicYN="N">quantitative real-time RT-PCR</Keyword><Keyword MajorTopicYN="N">β-glucuronidase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>11</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>04</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>04</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>6</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>6</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>3</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23746994</ArticleId><ArticleId IdType="pii">S0176-1617(13)00192-2</ArticleId><ArticleId IdType="doi">10.1016/j.jplph.2013.04.014</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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