Overexpression of bacterial γ-glutamylcysteine synthetase mediates changes in cadmium influx, allocation and detoxification in poplar.
Identifieur interne : 001F99 ( Main/Corpus ); précédent : 001F98; suivant : 002000Overexpression of bacterial γ-glutamylcysteine synthetase mediates changes in cadmium influx, allocation and detoxification in poplar.
Auteurs : Jiali He ; Hong Li ; Chaofeng Ma ; Yanli Zhang ; Andrea Polle ; Heinz Rennenberg ; Xingqi Cheng ; Zhi-Bin LuoSource :
- The New phytologist [ 1469-8137 ] ; 2015.
English descriptors
- KwdEn :
- Antioxidants (metabolism), Biological Transport (MeSH), Cadmium (metabolism), Calcium (metabolism), Carbohydrate Metabolism (genetics), Dipeptides (metabolism), Escherichia coli (enzymology), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Hydrogen (metabolism), Inactivation, Metabolic (MeSH), Models, Biological (MeSH), Plant Bark (metabolism), Plant Leaves (metabolism), Plant Roots (metabolism), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (growth & development), Populus (metabolism), Principal Component Analysis (MeSH), RNA, Messenger (genetics), RNA, Messenger (metabolism), Reactive Oxygen Species (metabolism), Sulfhydryl Compounds (metabolism), Superoxides (metabolism), Wood (metabolism).
- MESH :
- chemical , genetics : RNA, Messenger.
- chemical , metabolism : Antioxidants, Cadmium, Calcium, Dipeptides, Hydrogen, RNA, Messenger, Reactive Oxygen Species, Sulfhydryl Compounds, Superoxides.
- enzymology : Escherichia coli.
- genetics : Carbohydrate Metabolism, Populus.
- growth & development : Populus.
- metabolism : Plant Bark, Plant Leaves, Plant Roots, Populus, Wood.
- Biological Transport, Gene Expression Regulation, Plant, Genes, Plant, Inactivation, Metabolic, Models, Biological, Plants, Genetically Modified, Principal Component Analysis.
Abstract
Overexpression of bacterial γ-glutamylcysteine synthetase in the cytosol of Populus tremula × P. alba produces higher glutathione (GSH) concentrations in leaves, thereby indicating the potential for cadmium (Cd) phytoremediation. However, the net Cd(2+) influx in association with H(+) /Ca(2+) , Cd tolerance, and the underlying molecular and physiological mechanisms are uncharacterized in these poplars. We assessed net Cd(2+) influx, Cd tolerance and the transcriptional regulation of several genes involved in Cd(2+) transport and detoxification in wild-type and transgenic poplars. Poplars exhibited highest net Cd(2+) influxes into roots at pH 5.5 and 0.1 mM Ca(2+) . Transgenics had higher Cd(2+) uptake rates and elevated transcript levels of several genes involved in Cd(2+) transport and detoxification compared with wild-type poplars. Transgenics exhibited greater Cd accumulation in the aerial parts than wild-type plants in response to Cd(2+) exposure. Moreover, transgenic poplars had lower concentrations of O2 ˙(-) and H2 O2 ; higher concentrations of total thiols, GSH and oxidized GSH in roots and/or leaves; and stimulated foliar GSH reductase activity compared with wild-type plants. These results indicate that transgenics are more tolerant of 100 μM Cd(2+) than wild-type plants, probably due to the GSH-mediated induction of the transcription of genes involved in Cd(2+) transport and detoxification.
DOI: 10.1111/nph.13013
PubMed: 25229726
Links to Exploration step
pubmed:25229726Le document en format XML
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<affiliation><nlm:affiliation>College of Life Sciences and State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi, 712100, China; Department of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.</nlm:affiliation>
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<author><name sortKey="Li, Hong" sort="Li, Hong" uniqKey="Li H" first="Hong" last="Li">Hong Li</name>
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<author><name sortKey="Ma, Chaofeng" sort="Ma, Chaofeng" uniqKey="Ma C" first="Chaofeng" last="Ma">Chaofeng Ma</name>
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<author><name sortKey="Zhang, Yanli" sort="Zhang, Yanli" uniqKey="Zhang Y" first="Yanli" last="Zhang">Yanli Zhang</name>
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<author><name sortKey="Polle, Andrea" sort="Polle, Andrea" uniqKey="Polle A" first="Andrea" last="Polle">Andrea Polle</name>
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<author><name sortKey="Rennenberg, Heinz" sort="Rennenberg, Heinz" uniqKey="Rennenberg H" first="Heinz" last="Rennenberg">Heinz Rennenberg</name>
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<author><name sortKey="Cheng, Xingqi" sort="Cheng, Xingqi" uniqKey="Cheng X" first="Xingqi" last="Cheng">Xingqi Cheng</name>
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<affiliation><nlm:affiliation>College of Life Sciences and State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi, 712100, China; Department of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.</nlm:affiliation>
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<author><name sortKey="Ma, Chaofeng" sort="Ma, Chaofeng" uniqKey="Ma C" first="Chaofeng" last="Ma">Chaofeng Ma</name>
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<author><name sortKey="Zhang, Yanli" sort="Zhang, Yanli" uniqKey="Zhang Y" first="Yanli" last="Zhang">Yanli Zhang</name>
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<author><name sortKey="Polle, Andrea" sort="Polle, Andrea" uniqKey="Polle A" first="Andrea" last="Polle">Andrea Polle</name>
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<author><name sortKey="Rennenberg, Heinz" sort="Rennenberg, Heinz" uniqKey="Rennenberg H" first="Heinz" last="Rennenberg">Heinz Rennenberg</name>
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<series><title level="j">The New phytologist</title>
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<term>Biological Transport (MeSH)</term>
<term>Cadmium (metabolism)</term>
<term>Calcium (metabolism)</term>
<term>Carbohydrate Metabolism (genetics)</term>
<term>Dipeptides (metabolism)</term>
<term>Escherichia coli (enzymology)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genes, Plant (MeSH)</term>
<term>Hydrogen (metabolism)</term>
<term>Inactivation, Metabolic (MeSH)</term>
<term>Models, Biological (MeSH)</term>
<term>Plant Bark (metabolism)</term>
<term>Plant Leaves (metabolism)</term>
<term>Plant Roots (metabolism)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Populus (genetics)</term>
<term>Populus (growth & development)</term>
<term>Populus (metabolism)</term>
<term>Principal Component Analysis (MeSH)</term>
<term>RNA, Messenger (genetics)</term>
<term>RNA, Messenger (metabolism)</term>
<term>Reactive Oxygen Species (metabolism)</term>
<term>Sulfhydryl Compounds (metabolism)</term>
<term>Superoxides (metabolism)</term>
<term>Wood (metabolism)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Messenger</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antioxidants</term>
<term>Cadmium</term>
<term>Calcium</term>
<term>Dipeptides</term>
<term>Hydrogen</term>
<term>RNA, Messenger</term>
<term>Reactive Oxygen Species</term>
<term>Sulfhydryl Compounds</term>
<term>Superoxides</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Escherichia coli</term>
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<term>Populus</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Bark</term>
<term>Plant Leaves</term>
<term>Plant Roots</term>
<term>Populus</term>
<term>Wood</term>
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<keywords scheme="MESH" xml:lang="en"><term>Biological Transport</term>
<term>Gene Expression Regulation, Plant</term>
<term>Genes, Plant</term>
<term>Inactivation, Metabolic</term>
<term>Models, Biological</term>
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<front><div type="abstract" xml:lang="en">Overexpression of bacterial γ-glutamylcysteine synthetase in the cytosol of Populus tremula × P. alba produces higher glutathione (GSH) concentrations in leaves, thereby indicating the potential for cadmium (Cd) phytoremediation. However, the net Cd(2+) influx in association with H(+) /Ca(2+) , Cd tolerance, and the underlying molecular and physiological mechanisms are uncharacterized in these poplars. We assessed net Cd(2+) influx, Cd tolerance and the transcriptional regulation of several genes involved in Cd(2+) transport and detoxification in wild-type and transgenic poplars. Poplars exhibited highest net Cd(2+) influxes into roots at pH 5.5 and 0.1 mM Ca(2+) . Transgenics had higher Cd(2+) uptake rates and elevated transcript levels of several genes involved in Cd(2+) transport and detoxification compared with wild-type poplars. Transgenics exhibited greater Cd accumulation in the aerial parts than wild-type plants in response to Cd(2+) exposure. Moreover, transgenic poplars had lower concentrations of O2 ˙(-) and H2 O2 ; higher concentrations of total thiols, GSH and oxidized GSH in roots and/or leaves; and stimulated foliar GSH reductase activity compared with wild-type plants. These results indicate that transgenics are more tolerant of 100 μM Cd(2+) than wild-type plants, probably due to the GSH-mediated induction of the transcription of genes involved in Cd(2+) transport and detoxification.</div>
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<Abstract><AbstractText>Overexpression of bacterial γ-glutamylcysteine synthetase in the cytosol of Populus tremula × P. alba produces higher glutathione (GSH) concentrations in leaves, thereby indicating the potential for cadmium (Cd) phytoremediation. However, the net Cd(2+) influx in association with H(+) /Ca(2+) , Cd tolerance, and the underlying molecular and physiological mechanisms are uncharacterized in these poplars. We assessed net Cd(2+) influx, Cd tolerance and the transcriptional regulation of several genes involved in Cd(2+) transport and detoxification in wild-type and transgenic poplars. Poplars exhibited highest net Cd(2+) influxes into roots at pH 5.5 and 0.1 mM Ca(2+) . Transgenics had higher Cd(2+) uptake rates and elevated transcript levels of several genes involved in Cd(2+) transport and detoxification compared with wild-type poplars. Transgenics exhibited greater Cd accumulation in the aerial parts than wild-type plants in response to Cd(2+) exposure. Moreover, transgenic poplars had lower concentrations of O2 ˙(-) and H2 O2 ; higher concentrations of total thiols, GSH and oxidized GSH in roots and/or leaves; and stimulated foliar GSH reductase activity compared with wild-type plants. These results indicate that transgenics are more tolerant of 100 μM Cd(2+) than wild-type plants, probably due to the GSH-mediated induction of the transcription of genes involved in Cd(2+) transport and detoxification.</AbstractText>
<CopyrightInformation>© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.</CopyrightInformation>
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