Cryopreservation of dormant in vivo-buds of hybrid aspen: timing as a critical factor.
Identifieur interne : 001F25 ( Main/Corpus ); précédent : 001F24; suivant : 001F26Cryopreservation of dormant in vivo-buds of hybrid aspen: timing as a critical factor.
Auteurs : T. Aronen ; L. RyynanenSource :
- Cryo letters [ 0143-2044 ]
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Water.
- chemical , genetics : Plant Proteins.
- genetics : Plant Shoots, Populus.
- methods : Cryopreservation.
- physiology : Plant Shoots, Populus.
- Freezing, Gene Expression Regulation, Plant, Photoperiod, Temperature.
Abstract
BACKGROUND
For the conservation of hybrid aspen germplasm, cryostorage of dormant in vivo buds is a convenient back-up method for field collections. In practice in Finland, bud collection is performed from February to March.
OBJECTIVE
The aim of this study was to assess how this time schedule can be extended without compromising regeneration. In addition, an easily measurable marker for successful cryopreservation was examined.
MATERIALS AND METHODS
Timing of cryopreservation was tested from August to February, using dormant buds from both outdoor and indoor plants. To find a marker, water content and gene expression of hydrid aspens, as well as environmental factors such as temperature, temperature sum, and light period were followed.
RESULTS
Cryopreservation was successful from October to February, when, on an average, at least 75 % of the buds regenerated through micropropagation, and there was no difference to non-frozen controls. Significant genotypic variation was observed in October and February, with regeneration rates of 61-100 % and 37-98 %, respectively. No marker for successful cryopreservation was found among the studied factors.
CONCLUSION
The results provide flexibility for the undertaking of practical work, with a recommendation that cryopreservation can be carried out from November to January - earlier than the current practice.
PubMed: 25397953
Links to Exploration step
pubmed:25397953Le document en format XML
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<author><name sortKey="Aronen, T" sort="Aronen, T" uniqKey="Aronen T" first="T" last="Aronen">T. Aronen</name>
<affiliation><nlm:affiliation>Finnish Forest Research Institute, Punkaharju, Finland. tuija.aronen@metla.fi.</nlm:affiliation>
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<author><name sortKey="Ryynanen, L" sort="Ryynanen, L" uniqKey="Ryynanen L" first="L" last="Ryynanen">L. Ryynanen</name>
<affiliation><nlm:affiliation>Finnish Forest Research Institute, Punkaharju, Finland.</nlm:affiliation>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cryopreservation (methods)</term>
<term>Freezing (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Photoperiod (MeSH)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Shoots (genetics)</term>
<term>Plant Shoots (physiology)</term>
<term>Populus (genetics)</term>
<term>Populus (physiology)</term>
<term>Temperature (MeSH)</term>
<term>Water (analysis)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Water</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Shoots</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cryopreservation</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Plant Shoots</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Freezing</term>
<term>Gene Expression Regulation, Plant</term>
<term>Photoperiod</term>
<term>Temperature</term>
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<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>For the conservation of hybrid aspen germplasm, cryostorage of dormant in vivo buds is a convenient back-up method for field collections. In practice in Finland, bud collection is performed from February to March.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b>
</p>
<p>The aim of this study was to assess how this time schedule can be extended without compromising regeneration. In addition, an easily measurable marker for successful cryopreservation was examined.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>MATERIALS AND METHODS</b>
</p>
<p>Timing of cryopreservation was tested from August to February, using dormant buds from both outdoor and indoor plants. To find a marker, water content and gene expression of hydrid aspens, as well as environmental factors such as temperature, temperature sum, and light period were followed.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>Cryopreservation was successful from October to February, when, on an average, at least 75 % of the buds regenerated through micropropagation, and there was no difference to non-frozen controls. Significant genotypic variation was observed in October and February, with regeneration rates of 61-100 % and 37-98 %, respectively. No marker for successful cryopreservation was found among the studied factors.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSION</b>
</p>
<p>The results provide flexibility for the undertaking of practical work, with a recommendation that cryopreservation can be carried out from November to January - earlier than the current practice.</p>
</div>
</front>
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<DateRevised><Year>2014</Year>
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<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">For the conservation of hybrid aspen germplasm, cryostorage of dormant in vivo buds is a convenient back-up method for field collections. In practice in Finland, bud collection is performed from February to March.</AbstractText>
<AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">The aim of this study was to assess how this time schedule can be extended without compromising regeneration. In addition, an easily measurable marker for successful cryopreservation was examined.</AbstractText>
<AbstractText Label="MATERIALS AND METHODS" NlmCategory="METHODS">Timing of cryopreservation was tested from August to February, using dormant buds from both outdoor and indoor plants. To find a marker, water content and gene expression of hydrid aspens, as well as environmental factors such as temperature, temperature sum, and light period were followed.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Cryopreservation was successful from October to February, when, on an average, at least 75 % of the buds regenerated through micropropagation, and there was no difference to non-frozen controls. Significant genotypic variation was observed in October and February, with regeneration rates of 61-100 % and 37-98 %, respectively. No marker for successful cryopreservation was found among the studied factors.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The results provide flexibility for the undertaking of practical work, with a recommendation that cryopreservation can be carried out from November to January - earlier than the current practice.</AbstractText>
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