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Cryopreservation of dormant in vivo-buds of hybrid aspen: timing as a critical factor.

Identifieur interne : 001F25 ( Main/Corpus ); précédent : 001F24; suivant : 001F26

Cryopreservation of dormant in vivo-buds of hybrid aspen: timing as a critical factor.

Auteurs : T. Aronen ; L. Ryynanen

Source :

RBID : pubmed:25397953

English descriptors

Abstract

BACKGROUND

For the conservation of hybrid aspen germplasm, cryostorage of dormant in vivo buds is a convenient back-up method for field collections. In practice in Finland, bud collection is performed from February to March.

OBJECTIVE

The aim of this study was to assess how this time schedule can be extended without compromising regeneration. In addition, an easily measurable marker for successful cryopreservation was examined.

MATERIALS AND METHODS

Timing of cryopreservation was tested from August to February, using dormant buds from both outdoor and indoor plants. To find a marker, water content and gene expression of hydrid aspens, as well as environmental factors such as temperature, temperature sum, and light period were followed.

RESULTS

Cryopreservation was successful from October to February, when, on an average, at least 75 % of the buds regenerated through micropropagation, and there was no difference to non-frozen controls. Significant genotypic variation was observed in October and February, with regeneration rates of 61-100 % and 37-98 %, respectively. No marker for successful cryopreservation was found among the studied factors.

CONCLUSION

The results provide flexibility for the undertaking of practical work, with a recommendation that cryopreservation can be carried out from November to January - earlier than the current practice.


PubMed: 25397953

Links to Exploration step

pubmed:25397953

Le document en format XML

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<b>BACKGROUND</b>
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<p>For the conservation of hybrid aspen germplasm, cryostorage of dormant in vivo buds is a convenient back-up method for field collections. In practice in Finland, bud collection is performed from February to March.</p>
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<div type="abstract" xml:lang="en">
<p>
<b>OBJECTIVE</b>
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<p>The aim of this study was to assess how this time schedule can be extended without compromising regeneration. In addition, an easily measurable marker for successful cryopreservation was examined.</p>
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<div type="abstract" xml:lang="en">
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<b>MATERIALS AND METHODS</b>
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<p>Timing of cryopreservation was tested from August to February, using dormant buds from both outdoor and indoor plants. To find a marker, water content and gene expression of hydrid aspens, as well as environmental factors such as temperature, temperature sum, and light period were followed.</p>
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<b>RESULTS</b>
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<p>Cryopreservation was successful from October to February, when, on an average, at least 75 % of the buds regenerated through micropropagation, and there was no difference to non-frozen controls. Significant genotypic variation was observed in October and February, with regeneration rates of 61-100 % and 37-98 %, respectively. No marker for successful cryopreservation was found among the studied factors.</p>
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<b>CONCLUSION</b>
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<p>The results provide flexibility for the undertaking of practical work, with a recommendation that cryopreservation can be carried out from November to January - earlier than the current practice.</p>
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