Two WUSCHEL-related HOMEOBOX genes, PeWOX11a and PeWOX11b, are involved in adventitious root formation of poplar.
Identifieur interne : 001C81 ( Main/Corpus ); précédent : 001C80; suivant : 001C82Two WUSCHEL-related HOMEOBOX genes, PeWOX11a and PeWOX11b, are involved in adventitious root formation of poplar.
Auteurs : Meng Xu ; Wenfan Xie ; Minren HuangSource :
- Physiologia plantarum [ 1399-3054 ] ; 2015.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Cell Nucleus (genetics), Cell Nucleus (metabolism), Gene Expression Profiling (methods), Gene Expression Regulation, Developmental (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Homeobox (genetics), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Microscopy, Confocal (MeSH), Molecular Sequence Data (MeSH), Oligonucleotide Array Sequence Analysis (MeSH), Phylogeny (MeSH), Plant Proteins (classification), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Roots (genetics), Plant Roots (growth & development), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (growth & development), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Sequence Homology, Amino Acid (MeSH), Sequence Homology, Nucleic Acid (MeSH).
- MESH :
- chemical , classification : Plant Proteins.
- chemical , genetics : Green Fluorescent Proteins, Plant Proteins.
- genetics : Cell Nucleus, Genes, Homeobox, Plant Roots, Populus.
- growth & development : Plant Roots, Populus.
- metabolism : Cell Nucleus, Green Fluorescent Proteins, Plant Proteins.
- methods : Gene Expression Profiling.
- Amino Acid Sequence, Base Sequence, Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Microscopy, Confocal, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Phylogeny, Plants, Genetically Modified, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid.
Abstract
The plant-specific WUSCHEL-related HOMEOBOX (WOX) transcription factors play important roles in key developmental processes, but knowledge regarding functional characterization of WOX genes in poplar remains limited. To reveal genes and signaling pathways associated with adventitious rooting in poplar, here we isolated and characterized two WOX genes through the rapid amplification of cDNA ends (RACE), sequence aligning, expression profiling, protoplast transfection and poplar transformation. Detailed information about the sequence similarity, structural features, evolutionary relationships, expression patterns and subcellular localization of the two genes were revealed. Overexpression of either PeWOX11a or PeWOX11b not only increased the number of adventitious roots on the cuttings but also induced ectopic roots in the aerial parts of transgenic poplars. Meanwhile, their overexpression in transgenic poplars affected axillary bud and leaf development. These results suggest that PeWOX11a and PeWOX11b were involved in multiple developmental processes of poplar, especially in adventitious root formation. Our results provide new insights into the molecular mechanisms underlying adventitious root formation of poplar.
DOI: 10.1111/ppl.12349
PubMed: 25998748
Links to Exploration step
pubmed:25998748Le document en format XML
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Biotechnology, Nanjing Forestry University, Ministry of Education, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Huang, Minren" sort="Huang, Minren" uniqKey="Huang M" first="Minren" last="Huang">Minren Huang</name><affiliation><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Forest Genetics & Biotechnology, Nanjing Forestry University, Ministry of Education, Nanjing, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25998748</idno><idno type="pmid">25998748</idno><idno type="doi">10.1111/ppl.12349</idno><idno type="wicri:Area/Main/Corpus">001C81</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" 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China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Forest Genetics & Biotechnology, Nanjing Forestry University, Ministry of Education, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Huang, Minren" sort="Huang, Minren" uniqKey="Huang M" first="Minren" last="Huang">Minren Huang</name><affiliation><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Forest Genetics & Biotechnology, Nanjing Forestry University, Ministry of Education, Nanjing, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Physiologia plantarum</title><idno type="eISSN">1399-3054</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Cell Nucleus (genetics)</term><term>Cell Nucleus (metabolism)</term><term>Gene Expression Profiling (methods)</term><term>Gene Expression Regulation, Developmental (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Homeobox (genetics)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Microscopy, Confocal (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Oligonucleotide Array Sequence Analysis (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Proteins (classification)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plant Roots (genetics)</term><term>Plant Roots (growth & development)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Sequence Homology, Nucleic Acid (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Nucleus</term><term>Genes, Homeobox</term><term>Plant Roots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Roots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Nucleus</term><term>Green Fluorescent Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Gene Expression Profiling</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Gene Expression Regulation, Developmental</term><term>Gene Expression Regulation, Plant</term><term>Microscopy, Confocal</term><term>Molecular Sequence Data</term><term>Oligonucleotide Array Sequence Analysis</term><term>Phylogeny</term><term>Plants, Genetically Modified</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Homology, Amino Acid</term><term>Sequence Homology, Nucleic Acid</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The plant-specific WUSCHEL-related HOMEOBOX (WOX) transcription factors play important roles in key developmental processes, but knowledge regarding functional characterization of WOX genes in poplar remains limited. To reveal genes and signaling pathways associated with adventitious rooting in poplar, here we isolated and characterized two WOX genes through the rapid amplification of cDNA ends (RACE), sequence aligning, expression profiling, protoplast transfection and poplar transformation. Detailed information about the sequence similarity, structural features, evolutionary relationships, expression patterns and subcellular localization of the two genes were revealed. Overexpression of either PeWOX11a or PeWOX11b not only increased the number of adventitious roots on the cuttings but also induced ectopic roots in the aerial parts of transgenic poplars. Meanwhile, their overexpression in transgenic poplars affected axillary bud and leaf development. These results suggest that PeWOX11a and PeWOX11b were involved in multiple developmental processes of poplar, especially in adventitious root formation. Our results provide new insights into the molecular mechanisms underlying adventitious root formation of poplar. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25998748</PMID><DateCompleted><Year>2016</Year><Month>10</Month><Day>31</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1399-3054</ISSN><JournalIssue CitedMedium="Internet"><Volume>155</Volume><Issue>4</Issue><PubDate><Year>2015</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Physiologia plantarum</Title><ISOAbbreviation>Physiol Plant</ISOAbbreviation></Journal><ArticleTitle>Two WUSCHEL-related HOMEOBOX genes, PeWOX11a and PeWOX11b, are involved in adventitious root formation of poplar.</ArticleTitle><Pagination><MedlinePgn>446-56</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/ppl.12349</ELocationID><Abstract><AbstractText>The plant-specific WUSCHEL-related HOMEOBOX (WOX) transcription factors play important roles in key developmental processes, but knowledge regarding functional characterization of WOX genes in poplar remains limited. To reveal genes and signaling pathways associated with adventitious rooting in poplar, here we isolated and characterized two WOX genes through the rapid amplification of cDNA ends (RACE), sequence aligning, expression profiling, protoplast transfection and poplar transformation. Detailed information about the sequence similarity, structural features, evolutionary relationships, expression patterns and subcellular localization of the two genes were revealed. Overexpression of either PeWOX11a or PeWOX11b not only increased the number of adventitious roots on the cuttings but also induced ectopic roots in the aerial parts of transgenic poplars. Meanwhile, their overexpression in transgenic poplars affected axillary bud and leaf development. These results suggest that PeWOX11a and PeWOX11b were involved in multiple developmental processes of poplar, especially in adventitious root formation. Our results provide new insights into the molecular mechanisms underlying adventitious root formation of poplar. </AbstractText><CopyrightInformation>© 2015 Scandinavian Plant Physiology Society.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Meng</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Forest Genetics & Biotechnology, Nanjing Forestry University, Ministry of Education, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Wenfan</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Forest Genetics & Biotechnology, Nanjing Forestry University, Ministry of Education, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Minren</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Forest Genetics & Biotechnology, Nanjing Forestry University, Ministry of Education, Nanjing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>06</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>Physiol Plant</MedlineTA><NlmUniqueID>1256322</NlmUniqueID><ISSNLinking>0031-9317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" 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