FT overexpression induces precocious flowering and normal reproductive development in Eucalyptus.
Identifieur interne : 001C29 ( Main/Corpus ); précédent : 001C28; suivant : 001C30FT overexpression induces precocious flowering and normal reproductive development in Eucalyptus.
Auteurs : Amy L. Klocko ; Cathleen Ma ; Sarah Robertson ; Elahe Esfandiari ; Ove Nilsson ; Steven H. StraussSource :
- Plant biotechnology journal [ 1467-7652 ] ; 2016.
English descriptors
- KwdEn :
- Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), Crosses, Genetic (MeSH), Eucalyptus (anatomy & histology), Eucalyptus (genetics), Eucalyptus (growth & development), Flowers (anatomy & histology), Flowers (genetics), Flowers (growth & development), Gene Expression Regulation, Plant (MeSH), Germination (MeSH), Phenotype (MeSH), Pigmentation (MeSH), Plant Leaves (physiology), Plants, Genetically Modified (MeSH), Pollen (physiology), Pollination (MeSH), Reproduction (MeSH), Seeds (physiology), Self-Fertilization (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Arabidopsis Proteins.
- chemical , metabolism : Arabidopsis Proteins.
- anatomy & histology : Eucalyptus, Flowers.
- genetics : Eucalyptus, Flowers.
- growth & development : Eucalyptus, Flowers.
- physiology : Plant Leaves, Pollen, Seeds.
- Crosses, Genetic, Gene Expression Regulation, Plant, Germination, Phenotype, Pigmentation, Plants, Genetically Modified, Pollination, Reproduction, Self-Fertilization, Transformation, Genetic.
Abstract
Eucalyptus trees are among the most important species for industrial forestry worldwide. However, as with most forest trees, flowering does not begin for one to several years after planting which can limit the rate of conventional and molecular breeding. To speed flowering, we transformed a Eucalyptus grandis × urophylla hybrid (SP7) with a variety of constructs that enable overexpression of FLOWERING LOCUS T (FT). We found that FT expression led to very early flowering, with events showing floral buds within 1-5 months of transplanting to the glasshouse. The most rapid flowering was observed when the cauliflower mosaic virus 35S promoter was used to drive the Arabidopsis thaliana FT gene (AtFT). Early flowering was also observed with AtFT overexpression from a 409S ubiquitin promoter and under heat induction conditions with Populus trichocarpa FT1 (PtFT1) under control of a heat-shock promoter. Early flowering trees grew robustly, but exhibited a highly branched phenotype compared to the strong apical dominance of nonflowering transgenic and control trees. AtFT-induced flowers were morphologically normal and produced viable pollen grains and viable self- and cross-pollinated seeds. Many self-seedlings inherited AtFT and flowered early. FT overexpression-induced flowering in Eucalyptus may be a valuable means for accelerating breeding and genetic studies as the transgene can be easily segregated away in progeny, restoring normal growth and form.
DOI: 10.1111/pbi.12431
PubMed: 26132805
Links to Exploration step
pubmed:26132805Le document en format XML
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Klocko</name><affiliation><nlm:affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ma, Cathleen" sort="Ma, Cathleen" uniqKey="Ma C" first="Cathleen" last="Ma">Cathleen Ma</name><affiliation><nlm:affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Robertson, Sarah" sort="Robertson, Sarah" uniqKey="Robertson S" first="Sarah" last="Robertson">Sarah Robertson</name><affiliation><nlm:affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Esfandiari, Elahe" sort="Esfandiari, Elahe" uniqKey="Esfandiari E" first="Elahe" last="Esfandiari">Elahe Esfandiari</name><affiliation><nlm:affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Nilsson, Ove" sort="Nilsson, Ove" uniqKey="Nilsson O" first="Ove" last="Nilsson">Ove Nilsson</name><affiliation><nlm:affiliation>Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name><affiliation><nlm:affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26132805</idno><idno type="pmid">26132805</idno><idno type="doi">10.1111/pbi.12431</idno><idno type="wicri:Area/Main/Corpus">001C29</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001C29</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">FT overexpression induces precocious flowering and normal reproductive development in Eucalyptus.</title><author><name sortKey="Klocko, Amy L" sort="Klocko, Amy L" uniqKey="Klocko A" first="Amy L" last="Klocko">Amy L. Klocko</name><affiliation><nlm:affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ma, Cathleen" sort="Ma, Cathleen" uniqKey="Ma C" first="Cathleen" last="Ma">Cathleen Ma</name><affiliation><nlm:affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Robertson, Sarah" sort="Robertson, Sarah" uniqKey="Robertson S" first="Sarah" last="Robertson">Sarah Robertson</name><affiliation><nlm:affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Esfandiari, Elahe" sort="Esfandiari, Elahe" uniqKey="Esfandiari E" first="Elahe" last="Esfandiari">Elahe Esfandiari</name><affiliation><nlm:affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Nilsson, Ove" sort="Nilsson, Ove" uniqKey="Nilsson O" first="Ove" last="Nilsson">Ove Nilsson</name><affiliation><nlm:affiliation>Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name><affiliation><nlm:affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Plant biotechnology journal</title><idno type="eISSN">1467-7652</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis Proteins (genetics)</term><term>Arabidopsis Proteins (metabolism)</term><term>Crosses, Genetic (MeSH)</term><term>Eucalyptus (anatomy & histology)</term><term>Eucalyptus (genetics)</term><term>Eucalyptus (growth & development)</term><term>Flowers (anatomy & histology)</term><term>Flowers (genetics)</term><term>Flowers (growth & development)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Germination (MeSH)</term><term>Phenotype (MeSH)</term><term>Pigmentation (MeSH)</term><term>Plant Leaves (physiology)</term><term>Plants, Genetically Modified (MeSH)</term><term>Pollen (physiology)</term><term>Pollination (MeSH)</term><term>Reproduction (MeSH)</term><term>Seeds (physiology)</term><term>Self-Fertilization (MeSH)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Arabidopsis Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Arabidopsis Proteins</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Eucalyptus</term><term>Flowers</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Eucalyptus</term><term>Flowers</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Eucalyptus</term><term>Flowers</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Plant Leaves</term><term>Pollen</term><term>Seeds</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Crosses, Genetic</term><term>Gene Expression Regulation, Plant</term><term>Germination</term><term>Phenotype</term><term>Pigmentation</term><term>Plants, Genetically Modified</term><term>Pollination</term><term>Reproduction</term><term>Self-Fertilization</term><term>Transformation, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Eucalyptus trees are among the most important species for industrial forestry worldwide. However, as with most forest trees, flowering does not begin for one to several years after planting which can limit the rate of conventional and molecular breeding. To speed flowering, we transformed a Eucalyptus grandis × urophylla hybrid (SP7) with a variety of constructs that enable overexpression of FLOWERING LOCUS T (FT). We found that FT expression led to very early flowering, with events showing floral buds within 1-5 months of transplanting to the glasshouse. The most rapid flowering was observed when the cauliflower mosaic virus 35S promoter was used to drive the Arabidopsis thaliana FT gene (AtFT). Early flowering was also observed with AtFT overexpression from a 409S ubiquitin promoter and under heat induction conditions with Populus trichocarpa FT1 (PtFT1) under control of a heat-shock promoter. Early flowering trees grew robustly, but exhibited a highly branched phenotype compared to the strong apical dominance of nonflowering transgenic and control trees. AtFT-induced flowers were morphologically normal and produced viable pollen grains and viable self- and cross-pollinated seeds. Many self-seedlings inherited AtFT and flowered early. FT overexpression-induced flowering in Eucalyptus may be a valuable means for accelerating breeding and genetic studies as the transgene can be easily segregated away in progeny, restoring normal growth and form. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26132805</PMID><DateCompleted><Year>2016</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1467-7652</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><Issue>2</Issue><PubDate><Year>2016</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Plant biotechnology journal</Title><ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>FT overexpression induces precocious flowering and normal reproductive development in Eucalyptus.</ArticleTitle><Pagination><MedlinePgn>808-19</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/pbi.12431</ELocationID><Abstract><AbstractText>Eucalyptus trees are among the most important species for industrial forestry worldwide. However, as with most forest trees, flowering does not begin for one to several years after planting which can limit the rate of conventional and molecular breeding. To speed flowering, we transformed a Eucalyptus grandis × urophylla hybrid (SP7) with a variety of constructs that enable overexpression of FLOWERING LOCUS T (FT). We found that FT expression led to very early flowering, with events showing floral buds within 1-5 months of transplanting to the glasshouse. The most rapid flowering was observed when the cauliflower mosaic virus 35S promoter was used to drive the Arabidopsis thaliana FT gene (AtFT). Early flowering was also observed with AtFT overexpression from a 409S ubiquitin promoter and under heat induction conditions with Populus trichocarpa FT1 (PtFT1) under control of a heat-shock promoter. Early flowering trees grew robustly, but exhibited a highly branched phenotype compared to the strong apical dominance of nonflowering transgenic and control trees. AtFT-induced flowers were morphologically normal and produced viable pollen grains and viable self- and cross-pollinated seeds. Many self-seedlings inherited AtFT and flowered early. FT overexpression-induced flowering in Eucalyptus may be a valuable means for accelerating breeding and genetic studies as the transgene can be easily segregated away in progeny, restoring normal growth and form. </AbstractText><CopyrightInformation>© 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Klocko</LastName><ForeName>Amy L</ForeName><Initials>AL</Initials><AffiliationInfo><Affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Cathleen</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Robertson</LastName><ForeName>Sarah</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Esfandiari</LastName><ForeName>Elahe</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nilsson</LastName><ForeName>Ove</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Strauss</LastName><ForeName>Steven H</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>Department Forest Ecosystems & Society, Oregon State University, Corvallis, OR, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>At1g65480</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>07</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biotechnol J</MedlineTA><NlmUniqueID>101201889</NlmUniqueID><ISSNLinking>1467-7644</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029681">Arabidopsis Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C418905">FT protein, Arabidopsis</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D029681" MajorTopicYN="N">Arabidopsis Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003433" MajorTopicYN="N">Crosses, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005052" MajorTopicYN="N">Eucalyptus</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D035264" MajorTopicYN="N">Flowers</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018525" MajorTopicYN="N">Germination</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010858" MajorTopicYN="N">Pigmentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011058" MajorTopicYN="N">Pollen</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054817" MajorTopicYN="N">Pollination</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012098" MajorTopicYN="N">Reproduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057945" MajorTopicYN="N">Self-Fertilization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Eucalypts</Keyword><Keyword MajorTopicYN="N">Flowering Locus T</Keyword><Keyword MajorTopicYN="N">breeding</Keyword><Keyword MajorTopicYN="N">forest biotechnology</Keyword><Keyword MajorTopicYN="N">genetic engineering</Keyword><Keyword MajorTopicYN="N">transgenic</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>04</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>05</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>06</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>7</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>7</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26132805</ArticleId><ArticleId IdType="doi">10.1111/pbi.12431</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour 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