Identification of novel isoprene synthases through genome mining and expression in Escherichia coli.
Identifieur interne : 001B66 ( Main/Corpus ); précédent : 001B65; suivant : 001B67Identification of novel isoprene synthases through genome mining and expression in Escherichia coli.
Auteurs : Marja Ilmén ; Merja Oja ; Anne Huuskonen ; Sangmin Lee ; Laura Ruohonen ; Simon JungSource :
- Metabolic engineering [ 1096-7184 ] ; 2015.
English descriptors
- KwdEn :
- Alkyl and Aryl Transferases (chemistry), Alkyl and Aryl Transferases (isolation & purification), Alkyl and Aryl Transferases (physiology), Amino Acid Sequence (MeSH), Butadienes (MeSH), Escherichia coli (genetics), Genome, Bacterial (MeSH), Hemiterpenes (biosynthesis), Molecular Sequence Data (MeSH), Pentanes (MeSH), Sequence Homology (MeSH).
- MESH :
- chemical , biosynthesis : Hemiterpenes.
- chemical , chemistry : Alkyl and Aryl Transferases.
- chemical , isolation & purification : Alkyl and Aryl Transferases.
- chemical , physiology : Alkyl and Aryl Transferases.
- genetics : Escherichia coli.
- Amino Acid Sequence, Butadienes, Genome, Bacterial, Molecular Sequence Data, Pentanes, Sequence Homology.
Abstract
Isoprene is a naturally produced hydrocarbon emitted into the atmosphere by green plants. It is also a constituent of synthetic rubber and a potential biofuel. Microbial production of isoprene can become a sustainable alternative to the prevailing chemical production of isoprene from petroleum. In this work, sequence homology searches were conducted to find novel isoprene synthases. Candidate sequences were functionally expressed in Escherichia coli and the desired enzymes were identified based on an isoprene production assay. The activity of three enzymes was shown for the first time: expression of the candidate genes from Ipomoea batatas, Mangifera indica, and Elaeocarpus photiniifolius resulted in isoprene formation. The Ipomoea batatas isoprene synthase produced the highest amounts of isoprene in all experiments, exceeding the isoprene levels obtained by the previously known Populus alba and Pueraria montana isoprene synthases that were studied in parallel as controls.
DOI: 10.1016/j.ymben.2015.08.001
PubMed: 26275749
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pubmed:26275749Le document en format XML
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Box 1000, FI-02044 VTT, Espoo, Finland.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Sangmin" sort="Lee, Sangmin" uniqKey="Lee S" first="Sangmin" last="Lee">Sangmin Lee</name><affiliation><nlm:affiliation>Global Technology, SK Innovation, Daejeon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Ruohonen, Laura" sort="Ruohonen, Laura" uniqKey="Ruohonen L" first="Laura" last="Ruohonen">Laura Ruohonen</name><affiliation><nlm:affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</nlm:affiliation></affiliation></author><author><name sortKey="Jung, Simon" sort="Jung, Simon" uniqKey="Jung S" first="Simon" last="Jung">Simon Jung</name><affiliation><nlm:affiliation>Global Technology, SK Innovation, Daejeon, Republic of Korea. Electronic address: jungmoongeun@sk.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26275749</idno><idno type="pmid">26275749</idno><idno type="doi">10.1016/j.ymben.2015.08.001</idno><idno type="wicri:Area/Main/Corpus">001B66</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001B66</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of novel isoprene synthases through genome mining and expression in Escherichia coli.</title><author><name sortKey="Ilmen, Marja" sort="Ilmen, Marja" uniqKey="Ilmen M" first="Marja" last="Ilmén">Marja Ilmén</name><affiliation><nlm:affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</nlm:affiliation></affiliation></author><author><name sortKey="Oja, Merja" sort="Oja, Merja" uniqKey="Oja M" first="Merja" last="Oja">Merja Oja</name><affiliation><nlm:affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</nlm:affiliation></affiliation></author><author><name sortKey="Huuskonen, Anne" sort="Huuskonen, Anne" uniqKey="Huuskonen A" first="Anne" last="Huuskonen">Anne Huuskonen</name><affiliation><nlm:affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Sangmin" sort="Lee, Sangmin" uniqKey="Lee S" first="Sangmin" last="Lee">Sangmin Lee</name><affiliation><nlm:affiliation>Global Technology, SK Innovation, Daejeon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Ruohonen, Laura" sort="Ruohonen, Laura" uniqKey="Ruohonen L" first="Laura" last="Ruohonen">Laura Ruohonen</name><affiliation><nlm:affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</nlm:affiliation></affiliation></author><author><name sortKey="Jung, Simon" sort="Jung, Simon" uniqKey="Jung S" first="Simon" last="Jung">Simon Jung</name><affiliation><nlm:affiliation>Global Technology, SK Innovation, Daejeon, Republic of Korea. Electronic address: jungmoongeun@sk.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Metabolic engineering</title><idno type="eISSN">1096-7184</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkyl and Aryl Transferases (chemistry)</term><term>Alkyl and Aryl Transferases (isolation & purification)</term><term>Alkyl and Aryl Transferases (physiology)</term><term>Amino Acid Sequence (MeSH)</term><term>Butadienes (MeSH)</term><term>Escherichia coli (genetics)</term><term>Genome, Bacterial (MeSH)</term><term>Hemiterpenes (biosynthesis)</term><term>Molecular Sequence Data (MeSH)</term><term>Pentanes (MeSH)</term><term>Sequence Homology (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Hemiterpenes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Alkyl and Aryl Transferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Alkyl and Aryl Transferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Alkyl and Aryl Transferases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Butadienes</term><term>Genome, Bacterial</term><term>Molecular Sequence Data</term><term>Pentanes</term><term>Sequence Homology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Isoprene is a naturally produced hydrocarbon emitted into the atmosphere by green plants. It is also a constituent of synthetic rubber and a potential biofuel. Microbial production of isoprene can become a sustainable alternative to the prevailing chemical production of isoprene from petroleum. In this work, sequence homology searches were conducted to find novel isoprene synthases. Candidate sequences were functionally expressed in Escherichia coli and the desired enzymes were identified based on an isoprene production assay. The activity of three enzymes was shown for the first time: expression of the candidate genes from Ipomoea batatas, Mangifera indica, and Elaeocarpus photiniifolius resulted in isoprene formation. The Ipomoea batatas isoprene synthase produced the highest amounts of isoprene in all experiments, exceeding the isoprene levels obtained by the previously known Populus alba and Pueraria montana isoprene synthases that were studied in parallel as controls. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26275749</PMID><DateCompleted><Year>2016</Year><Month>09</Month><Day>09</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-7184</ISSN><JournalIssue CitedMedium="Internet"><Volume>31</Volume><PubDate><Year>2015</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Metabolic engineering</Title><ISOAbbreviation>Metab Eng</ISOAbbreviation></Journal><ArticleTitle>Identification of novel isoprene synthases through genome mining and expression in Escherichia coli.</ArticleTitle><Pagination><MedlinePgn>153-62</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ymben.2015.08.001</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1096-7176(15)00098-1</ELocationID><Abstract><AbstractText>Isoprene is a naturally produced hydrocarbon emitted into the atmosphere by green plants. It is also a constituent of synthetic rubber and a potential biofuel. Microbial production of isoprene can become a sustainable alternative to the prevailing chemical production of isoprene from petroleum. In this work, sequence homology searches were conducted to find novel isoprene synthases. Candidate sequences were functionally expressed in Escherichia coli and the desired enzymes were identified based on an isoprene production assay. The activity of three enzymes was shown for the first time: expression of the candidate genes from Ipomoea batatas, Mangifera indica, and Elaeocarpus photiniifolius resulted in isoprene formation. The Ipomoea batatas isoprene synthase produced the highest amounts of isoprene in all experiments, exceeding the isoprene levels obtained by the previously known Populus alba and Pueraria montana isoprene synthases that were studied in parallel as controls. </AbstractText><CopyrightInformation>Copyright © 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ilmén</LastName><ForeName>Marja</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oja</LastName><ForeName>Merja</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huuskonen</LastName><ForeName>Anne</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Sangmin</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Global Technology, SK Innovation, Daejeon, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ruohonen</LastName><ForeName>Laura</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jung</LastName><ForeName>Simon</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Global Technology, SK Innovation, Daejeon, Republic of Korea. Electronic address: jungmoongeun@sk.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>08</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Belgium</Country><MedlineTA>Metab Eng</MedlineTA><NlmUniqueID>9815657</NlmUniqueID><ISSNLinking>1096-7176</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002070">Butadienes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045782">Hemiterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010420">Pentanes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0A62964IBU</RegistryNumber><NameOfSubstance UI="C005059">isoprene</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.-</RegistryNumber><NameOfSubstance UI="D019883">Alkyl and Aryl Transferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.1.-</RegistryNumber><NameOfSubstance UI="C093854">isoprene synthase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019883" MajorTopicYN="N">Alkyl and Aryl Transferases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002070" MajorTopicYN="N">Butadienes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016680" MajorTopicYN="N">Genome, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045782" MajorTopicYN="N">Hemiterpenes</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010420" MajorTopicYN="N">Pentanes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017385" MajorTopicYN="N">Sequence Homology</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Genome mining</Keyword><Keyword MajorTopicYN="N">Homology-based screening</Keyword><Keyword MajorTopicYN="N">Isoprenoid</Keyword><Keyword MajorTopicYN="N">Terpene</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>01</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>06</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>08</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>8</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>8</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>9</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26275749</ArticleId><ArticleId IdType="pii">S1096-7176(15)00098-1</ArticleId><ArticleId IdType="doi">10.1016/j.ymben.2015.08.001</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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