Evaluation of a novel promoter from Populus trichocarpa for mature xylem tissue specific gene delivery.
Identifieur interne : 001850 ( Main/Corpus ); précédent : 001849; suivant : 001851Evaluation of a novel promoter from Populus trichocarpa for mature xylem tissue specific gene delivery.
Auteurs : Van Phap Nguyen ; Jin-Seong Cho ; Young-Im Choi ; Sang-Won Lee ; Kyung-Hwan Han ; Jae-Heung KoSource :
- Plant physiology and biochemistry : PPB [ 1873-2690 ] ; 2016.
English descriptors
- KwdEn :
- Arabidopsis (drug effects), Arabidopsis (genetics), Gene Expression Regulation, Plant (drug effects), Gene Transfer Techniques (MeSH), Glucuronidase (metabolism), Organ Specificity (drug effects), Organ Specificity (genetics), Plant Growth Regulators (pharmacology), Plants, Genetically Modified (MeSH), Populus (drug effects), Populus (genetics), Promoter Regions, Genetic (MeSH), Xylem (drug effects), Xylem (genetics).
- MESH :
- chemical , metabolism : Glucuronidase.
- drug effects : Arabidopsis, Gene Expression Regulation, Plant, Organ Specificity, Populus, Xylem.
- genetics : Arabidopsis, Organ Specificity, Populus, Xylem.
- chemical , pharmacology : Plant Growth Regulators.
- Gene Transfer Techniques, Plants, Genetically Modified, Promoter Regions, Genetic.
Abstract
Wood (i.e., secondary xylem) is an important raw material for many industrial applications. Mature xylem (MX) tissue-specific genetic modification offers an effective means to improve the chemical and physical properties of the wood. Here, we describe a promoter that drives strong gene expression in a MX tissue-specific manner. Using whole-transcriptome genechip analyses of different tissue types of poplar, we identified five candidate genes that had strong expression in the MX tissue. The putative promoter sequences of the five MX-specific genes were evaluated for their promoter activity in both transgenic Arabidopsis and poplar. Among them, we found the promoter of Potri.013G007900.1 (called the PtrMX3 promoter) had the strongest activity in MX and thus was further characterized. In the stem and root tissues of transgenic Arabidopsis plants, the PtrMX3 promoter activity was found exclusively in MX tissue. MX-specific activity of the promoter was reproduced in the stem tissue of transgenic poplar plants. The PtrMX3 promoter activity was not influenced by abiotic stresses or exogenously applied growth regulators, indicating the PtrMX3 promoter is bona fide MX tissue-specific. Our study provides a strong MX-specific promoter for MX-specific modifications of woody biomass.
DOI: 10.1016/j.plaphy.2016.03.033
PubMed: 27038601
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Electronic address: jhko@khu.ac.kr.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27038601</idno><idno type="pmid">27038601</idno><idno type="doi">10.1016/j.plaphy.2016.03.033</idno><idno type="wicri:Area/Main/Corpus">001850</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001850</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Evaluation of a novel promoter from Populus trichocarpa for mature xylem tissue specific gene delivery.</title><author><name sortKey="Nguyen, Van Phap" sort="Nguyen, Van Phap" uniqKey="Nguyen V" first="Van Phap" last="Nguyen">Van Phap Nguyen</name><affiliation><nlm:affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 17104, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Cho, Jin Seong" sort="Cho, Jin Seong" uniqKey="Cho J" first="Jin-Seong" last="Cho">Jin-Seong Cho</name><affiliation><nlm:affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 17104, Republic of Korea; Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 16631, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Choi, Young Im" sort="Choi, Young Im" uniqKey="Choi Y" first="Young-Im" last="Choi">Young-Im Choi</name><affiliation><nlm:affiliation>Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 16631, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Sang Won" sort="Lee, Sang Won" uniqKey="Lee S" first="Sang-Won" last="Lee">Sang-Won Lee</name><affiliation><nlm:affiliation>Department of Genetic Engineering & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Han, Kyung Hwan" sort="Han, Kyung Hwan" uniqKey="Han K" first="Kyung-Hwan" last="Han">Kyung-Hwan Han</name><affiliation><nlm:affiliation>Department of Horticulture and Department of Forestry, Michigan State University, East Lansing, MI 48824-1222, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ko, Jae Heung" sort="Ko, Jae Heung" uniqKey="Ko J" first="Jae-Heung" last="Ko">Jae-Heung Ko</name><affiliation><nlm:affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 17104, Republic of Korea. Electronic address: jhko@khu.ac.kr.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Plant physiology and biochemistry : PPB</title><idno type="eISSN">1873-2690</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (drug effects)</term><term>Arabidopsis (genetics)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Gene Transfer Techniques (MeSH)</term><term>Glucuronidase (metabolism)</term><term>Organ Specificity (drug effects)</term><term>Organ Specificity (genetics)</term><term>Plant Growth Regulators (pharmacology)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (drug effects)</term><term>Populus (genetics)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Xylem (drug effects)</term><term>Xylem (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucuronidase</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Arabidopsis</term><term>Gene Expression Regulation, Plant</term><term>Organ Specificity</term><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Organ Specificity</term><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Plant Growth Regulators</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Transfer Techniques</term><term>Plants, Genetically Modified</term><term>Promoter Regions, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Wood (i.e., secondary xylem) is an important raw material for many industrial applications. Mature xylem (MX) tissue-specific genetic modification offers an effective means to improve the chemical and physical properties of the wood. Here, we describe a promoter that drives strong gene expression in a MX tissue-specific manner. Using whole-transcriptome genechip analyses of different tissue types of poplar, we identified five candidate genes that had strong expression in the MX tissue. The putative promoter sequences of the five MX-specific genes were evaluated for their promoter activity in both transgenic Arabidopsis and poplar. Among them, we found the promoter of Potri.013G007900.1 (called the PtrMX3 promoter) had the strongest activity in MX and thus was further characterized. In the stem and root tissues of transgenic Arabidopsis plants, the PtrMX3 promoter activity was found exclusively in MX tissue. MX-specific activity of the promoter was reproduced in the stem tissue of transgenic poplar plants. The PtrMX3 promoter activity was not influenced by abiotic stresses or exogenously applied growth regulators, indicating the PtrMX3 promoter is bona fide MX tissue-specific. Our study provides a strong MX-specific promoter for MX-specific modifications of woody biomass. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27038601</PMID><DateCompleted><Year>2017</Year><Month>03</Month><Day>27</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2690</ISSN><JournalIssue CitedMedium="Internet"><Volume>104</Volume><PubDate><Year>2016</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Plant physiology and biochemistry : PPB</Title><ISOAbbreviation>Plant Physiol Biochem</ISOAbbreviation></Journal><ArticleTitle>Evaluation of a novel promoter from Populus trichocarpa for mature xylem tissue specific gene delivery.</ArticleTitle><Pagination><MedlinePgn>226-33</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plaphy.2016.03.033</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0981-9428(16)30102-4</ELocationID><Abstract><AbstractText>Wood (i.e., secondary xylem) is an important raw material for many industrial applications. Mature xylem (MX) tissue-specific genetic modification offers an effective means to improve the chemical and physical properties of the wood. Here, we describe a promoter that drives strong gene expression in a MX tissue-specific manner. Using whole-transcriptome genechip analyses of different tissue types of poplar, we identified five candidate genes that had strong expression in the MX tissue. The putative promoter sequences of the five MX-specific genes were evaluated for their promoter activity in both transgenic Arabidopsis and poplar. Among them, we found the promoter of Potri.013G007900.1 (called the PtrMX3 promoter) had the strongest activity in MX and thus was further characterized. In the stem and root tissues of transgenic Arabidopsis plants, the PtrMX3 promoter activity was found exclusively in MX tissue. MX-specific activity of the promoter was reproduced in the stem tissue of transgenic poplar plants. The PtrMX3 promoter activity was not influenced by abiotic stresses or exogenously applied growth regulators, indicating the PtrMX3 promoter is bona fide MX tissue-specific. Our study provides a strong MX-specific promoter for MX-specific modifications of woody biomass. </AbstractText><CopyrightInformation>Copyright © 2016 Elsevier Masson SAS. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nguyen</LastName><ForeName>Van Phap</ForeName><Initials>VP</Initials><AffiliationInfo><Affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 17104, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cho</LastName><ForeName>Jin-Seong</ForeName><Initials>JS</Initials><AffiliationInfo><Affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 17104, Republic of Korea; Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 16631, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Young-Im</ForeName><Initials>YI</Initials><AffiliationInfo><Affiliation>Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 16631, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Sang-Won</ForeName><Initials>SW</Initials><AffiliationInfo><Affiliation>Department of Genetic Engineering & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Kyung-Hwan</ForeName><Initials>KH</Initials><AffiliationInfo><Affiliation>Department of Horticulture and Department of Forestry, Michigan State University, East Lansing, MI 48824-1222, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ko</LastName><ForeName>Jae-Heung</ForeName><Initials>JH</Initials><AffiliationInfo><Affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 17104, Republic of Korea. Electronic address: jhko@khu.ac.kr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>03</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Plant Physiol Biochem</MedlineTA><NlmUniqueID>9882449</NlmUniqueID><ISSNLinking>0981-9428</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010937">Plant Growth Regulators</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018014" MajorTopicYN="Y">Gene Transfer Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009928" MajorTopicYN="N">Organ Specificity</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010937" MajorTopicYN="N">Plant Growth Regulators</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="Y">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biotechnology</Keyword><Keyword MajorTopicYN="N">Mature xylem</Keyword><Keyword MajorTopicYN="N">Poplar</Keyword><Keyword MajorTopicYN="N">Tissue-specific promoter</Keyword><Keyword MajorTopicYN="N">Woody biomass</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>01</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>03</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>03</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>4</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>4</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>3</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27038601</ArticleId><ArticleId IdType="pii">S0981-9428(16)30102-4</ArticleId><ArticleId IdType="doi">10.1016/j.plaphy.2016.03.033</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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