MiR169 and its target PagHAP2-6 regulated by ABA are involved in poplar cambium dormancy.
Identifieur interne : 001822 ( Main/Corpus ); précédent : 001821; suivant : 001823MiR169 and its target PagHAP2-6 regulated by ABA are involved in poplar cambium dormancy.
Auteurs : Qi Ding ; Jun Zeng ; Xin-Qiang HeSource :
- Journal of plant physiology [ 1618-1328 ] ; 2016.
English descriptors
- KwdEn :
- Abscisic Acid (pharmacology), Base Sequence (MeSH), Cambium (drug effects), Cambium (genetics), Cloning, Molecular (MeSH), Gene Expression Regulation, Plant (drug effects), Genes, Plant (MeSH), Genetic Complementation Test (MeSH), MicroRNAs (genetics), MicroRNAs (metabolism), Plant Dormancy (drug effects), Plant Dormancy (genetics), Plant Leaves (drug effects), Plant Leaves (genetics), Plant Leaves (growth & development), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (drug effects), Populus (genetics), Saccharomyces cerevisiae (metabolism), Transcription, Genetic (drug effects).
- MESH :
- chemical , chemistry : Plant Proteins.
- chemical , genetics : MicroRNAs, Plant Proteins.
- chemical , metabolism : MicroRNAs, Plant Proteins.
- chemical , pharmacology : Abscisic Acid.
- drug effects : Cambium, Gene Expression Regulation, Plant, Plant Dormancy, Plant Leaves, Populus, Transcription, Genetic.
- genetics : Cambium, Plant Dormancy, Plant Leaves, Populus.
- growth & development : Plant Leaves.
- metabolism : Saccharomyces cerevisiae.
- Base Sequence, Cloning, Molecular, Genes, Plant, Genetic Complementation Test.
Abstract
Dormancy is an effective strategy for perennial plants in temperate zones to survive the winter stress. MicroRNAs (miRNAs) have been well known as important regulators for various biological processes. In this study, we checked the expression of miR169 members in the cambium zone during dormancy and active growth in poplar and found that they had distinct expression patterns. We identified and characterized a dormancy-specific target gene of miR169, PagHAP2-6. 5' RACE assays confirmed the direct cleavage of PagHAP2-6 mRNA by miR169. The yeast functional complementation analysis showed that PagHAP2-6 was a homolog of Heme Activator Protein2 (HAP2)/Nuclear factor Y-A (NF-YA) transcription factor in poplar. qRT-PCR analysis indicated that PagHAP2-6 was highly expressed in the dormant stage, which was converse to the expression pattern of pag-miR169a, n, and r. In addition, the transcription of PagHAP2-6 was induced by exogenous abscisic acid (ABA), and both over-expression of PagHAP2-6 in Arabidopsis and transient co-expression assays in Nicotiana benthamiana indicated that PagHAP2-6 could increase the resistance to exogenous ABA. Taken together, the results suggested that miR169 and its target PagHAP2-6 regulated by ABA were involved in poplar cambium dormancy, which provided new insights into the regulatory mechanisms of tree dormancy-active growth transition.
DOI: 10.1016/j.jplph.2016.03.017
PubMed: 27111502
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">MiR169 and its target PagHAP2-6 regulated by ABA are involved in poplar cambium dormancy.</title><author><name sortKey="Ding, Qi" sort="Ding, Qi" uniqKey="Ding Q" first="Qi" last="Ding">Qi Ding</name><affiliation><nlm:affiliation>State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zeng, Jun" sort="Zeng, Jun" uniqKey="Zeng J" first="Jun" last="Zeng">Jun Zeng</name><affiliation><nlm:affiliation>State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.</nlm:affiliation></affiliation></author><author><name sortKey="He, Xin Qiang" sort="He, Xin Qiang" uniqKey="He X" first="Xin-Qiang" last="He">Xin-Qiang He</name><affiliation><nlm:affiliation>State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China. Electronic address: hexq@pku.edu.cn.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27111502</idno><idno type="pmid">27111502</idno><idno type="doi">10.1016/j.jplph.2016.03.017</idno><idno type="wicri:Area/Main/Corpus">001822</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001822</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">MiR169 and its target PagHAP2-6 regulated by ABA are involved in poplar cambium dormancy.</title><author><name sortKey="Ding, Qi" sort="Ding, Qi" uniqKey="Ding Q" first="Qi" last="Ding">Qi Ding</name><affiliation><nlm:affiliation>State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zeng, Jun" sort="Zeng, Jun" uniqKey="Zeng J" first="Jun" last="Zeng">Jun Zeng</name><affiliation><nlm:affiliation>State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.</nlm:affiliation></affiliation></author><author><name sortKey="He, Xin Qiang" sort="He, Xin Qiang" uniqKey="He X" first="Xin-Qiang" last="He">Xin-Qiang He</name><affiliation><nlm:affiliation>State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China. Electronic address: hexq@pku.edu.cn.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of plant physiology</title><idno type="eISSN">1618-1328</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abscisic Acid (pharmacology)</term><term>Base Sequence (MeSH)</term><term>Cambium (drug effects)</term><term>Cambium (genetics)</term><term>Cloning, Molecular (MeSH)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Genes, Plant (MeSH)</term><term>Genetic Complementation Test (MeSH)</term><term>MicroRNAs (genetics)</term><term>MicroRNAs (metabolism)</term><term>Plant Dormancy (drug effects)</term><term>Plant Dormancy (genetics)</term><term>Plant Leaves (drug effects)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (growth & development)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Populus (drug effects)</term><term>Populus (genetics)</term><term>Saccharomyces cerevisiae (metabolism)</term><term>Transcription, Genetic (drug effects)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>MicroRNAs</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>MicroRNAs</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Abscisic Acid</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cambium</term><term>Gene Expression Regulation, Plant</term><term>Plant Dormancy</term><term>Plant Leaves</term><term>Populus</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cambium</term><term>Plant Dormancy</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Cloning, Molecular</term><term>Genes, Plant</term><term>Genetic Complementation Test</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Dormancy is an effective strategy for perennial plants in temperate zones to survive the winter stress. MicroRNAs (miRNAs) have been well known as important regulators for various biological processes. In this study, we checked the expression of miR169 members in the cambium zone during dormancy and active growth in poplar and found that they had distinct expression patterns. We identified and characterized a dormancy-specific target gene of miR169, PagHAP2-6. 5' RACE assays confirmed the direct cleavage of PagHAP2-6 mRNA by miR169. The yeast functional complementation analysis showed that PagHAP2-6 was a homolog of Heme Activator Protein2 (HAP2)/Nuclear factor Y-A (NF-YA) transcription factor in poplar. qRT-PCR analysis indicated that PagHAP2-6 was highly expressed in the dormant stage, which was converse to the expression pattern of pag-miR169a, n, and r. In addition, the transcription of PagHAP2-6 was induced by exogenous abscisic acid (ABA), and both over-expression of PagHAP2-6 in Arabidopsis and transient co-expression assays in Nicotiana benthamiana indicated that PagHAP2-6 could increase the resistance to exogenous ABA. Taken together, the results suggested that miR169 and its target PagHAP2-6 regulated by ABA were involved in poplar cambium dormancy, which provided new insights into the regulatory mechanisms of tree dormancy-active growth transition. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27111502</PMID><DateCompleted><Year>2017</Year><Month>04</Month><Day>10</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-1328</ISSN><JournalIssue CitedMedium="Internet"><Volume>198</Volume><PubDate><Year>2016</Year><Month>Jul</Month><Day>01</Day></PubDate></JournalIssue><Title>Journal of plant physiology</Title><ISOAbbreviation>J Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>MiR169 and its target PagHAP2-6 regulated by ABA are involved in poplar cambium dormancy.</ArticleTitle><Pagination><MedlinePgn>1-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jplph.2016.03.017</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0176-1617(16)30023-2</ELocationID><Abstract><AbstractText>Dormancy is an effective strategy for perennial plants in temperate zones to survive the winter stress. MicroRNAs (miRNAs) have been well known as important regulators for various biological processes. In this study, we checked the expression of miR169 members in the cambium zone during dormancy and active growth in poplar and found that they had distinct expression patterns. We identified and characterized a dormancy-specific target gene of miR169, PagHAP2-6. 5' RACE assays confirmed the direct cleavage of PagHAP2-6 mRNA by miR169. The yeast functional complementation analysis showed that PagHAP2-6 was a homolog of Heme Activator Protein2 (HAP2)/Nuclear factor Y-A (NF-YA) transcription factor in poplar. qRT-PCR analysis indicated that PagHAP2-6 was highly expressed in the dormant stage, which was converse to the expression pattern of pag-miR169a, n, and r. In addition, the transcription of PagHAP2-6 was induced by exogenous abscisic acid (ABA), and both over-expression of PagHAP2-6 in Arabidopsis and transient co-expression assays in Nicotiana benthamiana indicated that PagHAP2-6 could increase the resistance to exogenous ABA. Taken together, the results suggested that miR169 and its target PagHAP2-6 regulated by ABA were involved in poplar cambium dormancy, which provided new insights into the regulatory mechanisms of tree dormancy-active growth transition. </AbstractText><CopyrightInformation>Copyright © 2016 Elsevier GmbH. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Qi</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Jun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Xin-Qiang</ForeName><Initials>XQ</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China. Electronic address: hexq@pku.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>04</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Plant Physiol</MedlineTA><NlmUniqueID>9882059</NlmUniqueID><ISSNLinking>0176-1617</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D035683">MicroRNAs</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>72S9A8J5GW</RegistryNumber><NameOfSubstance UI="D000040">Abscisic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000040" MajorTopicYN="N">Abscisic Acid</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D058506" MajorTopicYN="N">Cambium</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005816" MajorTopicYN="N">Genetic Complementation Test</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D035683" MajorTopicYN="N">MicroRNAs</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057445" MajorTopicYN="N">Plant Dormancy</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ABA</Keyword><Keyword MajorTopicYN="N">Cambium</Keyword><Keyword MajorTopicYN="N">Dormancy</Keyword><Keyword MajorTopicYN="N">PagHAP2-6</Keyword><Keyword MajorTopicYN="N">miR169</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>09</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>03</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>03</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>4</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>4</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>4</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27111502</ArticleId><ArticleId IdType="pii">S0176-1617(16)30023-2</ArticleId><ArticleId 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