Poplar-Root Knot Nematode Interaction: A Model for Perennial Woody Species.
Identifieur interne : 001809 ( Main/Corpus ); précédent : 001808; suivant : 001810Poplar-Root Knot Nematode Interaction: A Model for Perennial Woody Species.
Auteurs : Fabien Baldacci-Cresp ; Pierre-Yves Sacré ; Laure Twyffels ; Adeline Mol ; Marjorie Vermeersch ; Eric Ziemons ; Philippe Hubert ; David Pérez-Morga ; Mondher El Jaziri ; Janice De Almeida Engler ; Marie BaucherSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- cytology : Populus, Tylenchoidea.
- parasitology : Plant Diseases, Plant Leaves, Plant Roots, Populus, Xylem.
- physiology : Tylenchoidea.
- Animals, Host-Pathogen Interactions.
Abstract
Plant root-knot nematode (RKN) interaction studies are performed on several host plant models. Though RKN interact with trees, no perennial woody model has been explored so far. Here, we show that poplar (Populus tremula × P. alba) grown in vitro is susceptible to Meloidogyne incognita, allowing this nematode to penetrate, to induce feeding sites, and to successfully complete its life cycle. Quantitative reverse transcription-polymerase chain reaction analysis was performed to study changes in poplar gene expression in galls compared with noninfected roots. Three genes (expansin A, histone 3.1, and asparagine synthase), selected as gall development marker genes, followed, during poplar-nematode interaction, a similar expression pattern to what was described for other plant hosts. Downregulation of four genes implicated in the monolignol biosynthesis pathway was evidenced in galls, suggesting a shift in the phenolic profile within galls developed on poplar roots. Raman microspectroscopy demonstrated that cell walls of giant cells were not lignified but mainly composed of pectin and cellulose. The data presented here suggest that RKN exercise conserved strategies to reproduce and to invade perennial plant species and that poplar is a suitable model host to study specific traits of tree-nematode interactions.
DOI: 10.1094/MPMI-01-16-0015-R
PubMed: 27135257
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Poplar-Root Knot Nematode Interaction: A Model for Perennial Woody Species.</title><author><name sortKey="Baldacci Cresp, Fabien" sort="Baldacci Cresp, Fabien" uniqKey="Baldacci Cresp F" first="Fabien" last="Baldacci-Cresp">Fabien Baldacci-Cresp</name><affiliation><nlm:affiliation>1 Laboratoire de Biotechnologie Végétale, Université libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, B-6041 Gosselies, Belgium;</nlm:affiliation></affiliation></author><author><name sortKey="Sacre, Pierre Yves" sort="Sacre, Pierre Yves" uniqKey="Sacre P" first="Pierre-Yves" last="Sacré">Pierre-Yves Sacré</name><affiliation><nlm:affiliation>2 University of Liege, CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, B36, B-4000 Liege, Belgium;</nlm:affiliation></affiliation></author><author><name sortKey="Twyffels, Laure" sort="Twyffels, Laure" uniqKey="Twyffels L" first="Laure" last="Twyffels">Laure Twyffels</name><affiliation><nlm:affiliation>3 Center for Microscopy and Molecular Imaging-CMMI, Université libre de Bruxelles;</nlm:affiliation></affiliation></author><author><name sortKey="Mol, Adeline" sort="Mol, Adeline" uniqKey="Mol A" first="Adeline" last="Mol">Adeline Mol</name><affiliation><nlm:affiliation>1 Laboratoire de Biotechnologie Végétale, Université libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, B-6041 Gosselies, Belgium;</nlm:affiliation></affiliation></author><author><name sortKey="Vermeersch, Marjorie" sort="Vermeersch, Marjorie" uniqKey="Vermeersch M" first="Marjorie" last="Vermeersch">Marjorie Vermeersch</name><affiliation><nlm:affiliation>3 Center for Microscopy and Molecular Imaging-CMMI, Université libre de Bruxelles;</nlm:affiliation></affiliation></author><author><name sortKey="Ziemons, Eric" sort="Ziemons, Eric" uniqKey="Ziemons E" first="Eric" last="Ziemons">Eric Ziemons</name><affiliation><nlm:affiliation>2 University of Liege, CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, B36, B-4000 Liege, Belgium;</nlm:affiliation></affiliation></author><author><name sortKey="Hubert, Philippe" sort="Hubert, Philippe" uniqKey="Hubert P" first="Philippe" last="Hubert">Philippe Hubert</name><affiliation><nlm:affiliation>2 University of Liege, CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, B36, B-4000 Liege, Belgium;</nlm:affiliation></affiliation></author><author><name sortKey="Perez Morga, David" sort="Perez Morga, David" uniqKey="Perez Morga D" first="David" last="Pérez-Morga">David Pérez-Morga</name><affiliation><nlm:affiliation>3 Center for Microscopy and Molecular Imaging-CMMI, Université libre de Bruxelles;</nlm:affiliation></affiliation><affiliation><nlm:affiliation>4 Laboratoire de Parasitologie Moléculaire, Université libre de Bruxelles; and.</nlm:affiliation></affiliation></author><author><name sortKey="El Jaziri, Mondher" sort="El Jaziri, Mondher" uniqKey="El Jaziri M" first="Mondher" last="El Jaziri">Mondher El Jaziri</name><affiliation><nlm:affiliation>1 Laboratoire de Biotechnologie Végétale, Université libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, B-6041 Gosselies, Belgium;</nlm:affiliation></affiliation></author><author><name sortKey="De Almeida Engler, Janice" sort="De Almeida Engler, Janice" uniqKey="De Almeida Engler J" first="Janice" last="De Almeida Engler">Janice De Almeida Engler</name><affiliation><nlm:affiliation>5 INRA, Université Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, F-06900 Sophia Antipolis, France.</nlm:affiliation></affiliation></author><author><name sortKey="Baucher, Marie" sort="Baucher, Marie" uniqKey="Baucher M" first="Marie" last="Baucher">Marie Baucher</name><affiliation><nlm:affiliation>1 Laboratoire de Biotechnologie Végétale, Université libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, B-6041 Gosselies, Belgium;</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27135257</idno><idno type="pmid">27135257</idno><idno type="doi">10.1094/MPMI-01-16-0015-R</idno><idno type="wicri:Area/Main/Corpus">001809</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001809</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Poplar-Root Knot Nematode Interaction: A Model for Perennial Woody Species.</title><author><name sortKey="Baldacci Cresp, Fabien" sort="Baldacci Cresp, Fabien" uniqKey="Baldacci Cresp F" first="Fabien" last="Baldacci-Cresp">Fabien Baldacci-Cresp</name><affiliation><nlm:affiliation>1 Laboratoire de Biotechnologie Végétale, Université libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, B-6041 Gosselies, Belgium;</nlm:affiliation></affiliation></author><author><name sortKey="Sacre, Pierre Yves" sort="Sacre, Pierre Yves" uniqKey="Sacre P" first="Pierre-Yves" last="Sacré">Pierre-Yves Sacré</name><affiliation><nlm:affiliation>2 University of Liege, CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, B36, B-4000 Liege, Belgium;</nlm:affiliation></affiliation></author><author><name sortKey="Twyffels, Laure" sort="Twyffels, Laure" uniqKey="Twyffels L" first="Laure" last="Twyffels">Laure Twyffels</name><affiliation><nlm:affiliation>3 Center for Microscopy and Molecular Imaging-CMMI, Université libre de Bruxelles;</nlm:affiliation></affiliation></author><author><name sortKey="Mol, Adeline" sort="Mol, Adeline" uniqKey="Mol A" first="Adeline" last="Mol">Adeline Mol</name><affiliation><nlm:affiliation>1 Laboratoire de Biotechnologie Végétale, Université libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, B-6041 Gosselies, Belgium;</nlm:affiliation></affiliation></author><author><name sortKey="Vermeersch, Marjorie" sort="Vermeersch, Marjorie" uniqKey="Vermeersch M" first="Marjorie" last="Vermeersch">Marjorie Vermeersch</name><affiliation><nlm:affiliation>3 Center for Microscopy and Molecular Imaging-CMMI, Université libre de Bruxelles;</nlm:affiliation></affiliation></author><author><name sortKey="Ziemons, Eric" sort="Ziemons, Eric" uniqKey="Ziemons E" first="Eric" last="Ziemons">Eric Ziemons</name><affiliation><nlm:affiliation>2 University of Liege, CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, B36, B-4000 Liege, Belgium;</nlm:affiliation></affiliation></author><author><name sortKey="Hubert, Philippe" sort="Hubert, Philippe" uniqKey="Hubert P" first="Philippe" last="Hubert">Philippe Hubert</name><affiliation><nlm:affiliation>2 University of Liege, CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, B36, B-4000 Liege, Belgium;</nlm:affiliation></affiliation></author><author><name sortKey="Perez Morga, David" sort="Perez Morga, David" uniqKey="Perez Morga D" first="David" last="Pérez-Morga">David Pérez-Morga</name><affiliation><nlm:affiliation>3 Center for Microscopy and Molecular Imaging-CMMI, Université libre de Bruxelles;</nlm:affiliation></affiliation><affiliation><nlm:affiliation>4 Laboratoire de Parasitologie Moléculaire, Université libre de Bruxelles; and.</nlm:affiliation></affiliation></author><author><name sortKey="El Jaziri, Mondher" sort="El Jaziri, Mondher" uniqKey="El Jaziri M" first="Mondher" last="El Jaziri">Mondher El Jaziri</name><affiliation><nlm:affiliation>1 Laboratoire de Biotechnologie Végétale, Université libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, B-6041 Gosselies, Belgium;</nlm:affiliation></affiliation></author><author><name sortKey="De Almeida Engler, Janice" sort="De Almeida Engler, Janice" uniqKey="De Almeida Engler J" first="Janice" last="De Almeida Engler">Janice De Almeida Engler</name><affiliation><nlm:affiliation>5 INRA, Université Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, F-06900 Sophia Antipolis, France.</nlm:affiliation></affiliation></author><author><name sortKey="Baucher, Marie" sort="Baucher, Marie" uniqKey="Baucher M" first="Marie" last="Baucher">Marie Baucher</name><affiliation><nlm:affiliation>1 Laboratoire de Biotechnologie Végétale, Université libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, B-6041 Gosselies, Belgium;</nlm:affiliation></affiliation></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Host-Pathogen Interactions (MeSH)</term><term>Plant Diseases (parasitology)</term><term>Plant Leaves (parasitology)</term><term>Plant Roots (parasitology)</term><term>Populus (cytology)</term><term>Populus (parasitology)</term><term>Tylenchoidea (cytology)</term><term>Tylenchoidea (physiology)</term><term>Xylem (parasitology)</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Populus</term><term>Tylenchoidea</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Plant Roots</term><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Tylenchoidea</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Host-Pathogen Interactions</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plant root-knot nematode (RKN) interaction studies are performed on several host plant models. Though RKN interact with trees, no perennial woody model has been explored so far. Here, we show that poplar (Populus tremula × P. alba) grown in vitro is susceptible to Meloidogyne incognita, allowing this nematode to penetrate, to induce feeding sites, and to successfully complete its life cycle. Quantitative reverse transcription-polymerase chain reaction analysis was performed to study changes in poplar gene expression in galls compared with noninfected roots. Three genes (expansin A, histone 3.1, and asparagine synthase), selected as gall development marker genes, followed, during poplar-nematode interaction, a similar expression pattern to what was described for other plant hosts. Downregulation of four genes implicated in the monolignol biosynthesis pathway was evidenced in galls, suggesting a shift in the phenolic profile within galls developed on poplar roots. Raman microspectroscopy demonstrated that cell walls of giant cells were not lignified but mainly composed of pectin and cellulose. The data presented here suggest that RKN exercise conserved strategies to reproduce and to invade perennial plant species and that poplar is a suitable model host to study specific traits of tree-nematode interactions. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27135257</PMID><DateCompleted><Year>2016</Year><Month>09</Month><Day>08</Day></DateCompleted><DateRevised><Year>2016</Year><Month>07</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>29</Volume><Issue>7</Issue><PubDate><Year>2016</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Poplar-Root Knot Nematode Interaction: A Model for Perennial Woody Species.</ArticleTitle><Pagination><MedlinePgn>560-72</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-01-16-0015-R</ELocationID><Abstract><AbstractText>Plant root-knot nematode (RKN) interaction studies are performed on several host plant models. Though RKN interact with trees, no perennial woody model has been explored so far. Here, we show that poplar (Populus tremula × P. alba) grown in vitro is susceptible to Meloidogyne incognita, allowing this nematode to penetrate, to induce feeding sites, and to successfully complete its life cycle. Quantitative reverse transcription-polymerase chain reaction analysis was performed to study changes in poplar gene expression in galls compared with noninfected roots. Three genes (expansin A, histone 3.1, and asparagine synthase), selected as gall development marker genes, followed, during poplar-nematode interaction, a similar expression pattern to what was described for other plant hosts. Downregulation of four genes implicated in the monolignol biosynthesis pathway was evidenced in galls, suggesting a shift in the phenolic profile within galls developed on poplar roots. Raman microspectroscopy demonstrated that cell walls of giant cells were not lignified but mainly composed of pectin and cellulose. The data presented here suggest that RKN exercise conserved strategies to reproduce and to invade perennial plant species and that poplar is a suitable model host to study specific traits of tree-nematode interactions. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Baldacci-Cresp</LastName><ForeName>Fabien</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>1 Laboratoire de Biotechnologie Végétale, Université libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, B-6041 Gosselies, Belgium;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sacré</LastName><ForeName>Pierre-Yves</ForeName><Initials>PY</Initials><AffiliationInfo><Affiliation>2 University of Liege, CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, B36, B-4000 Liege, Belgium;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Twyffels</LastName><ForeName>Laure</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>3 Center for Microscopy and Molecular Imaging-CMMI, Université libre de Bruxelles;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mol</LastName><ForeName>Adeline</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>1 Laboratoire de Biotechnologie Végétale, Université libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, B-6041 Gosselies, Belgium;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vermeersch</LastName><ForeName>Marjorie</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>3 Center for Microscopy and Molecular Imaging-CMMI, Université libre de Bruxelles;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ziemons</LastName><ForeName>Eric</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>2 University of Liege, CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, B36, B-4000 Liege, Belgium;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hubert</LastName><ForeName>Philippe</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>2 University of Liege, CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, B36, B-4000 Liege, Belgium;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pérez-Morga</LastName><ForeName>David</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>3 Center for Microscopy and Molecular Imaging-CMMI, Université libre de Bruxelles;</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>4 Laboratoire de Parasitologie Moléculaire, Université libre de Bruxelles; and.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>El Jaziri</LastName><ForeName>Mondher</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>1 Laboratoire de Biotechnologie Végétale, Université libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, B-6041 Gosselies, Belgium;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Almeida Engler</LastName><ForeName>Janice</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>5 INRA, Université Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, F-06900 Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Baucher</LastName><ForeName>Marie</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>1 Laboratoire de Biotechnologie Végétale, Université libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, B-6041 Gosselies, Belgium;</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>06</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="Y">Host-Pathogen Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014431" MajorTopicYN="N">Tylenchoidea</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>5</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>5</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>9</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27135257</ArticleId><ArticleId IdType="doi">10.1094/MPMI-01-16-0015-R</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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