PoplarV1, Main, Corpus, bibRecord, 001602

Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007.

Identifieur interne : 001602 ( Main/Corpus ); précédent : 001601; suivant : 001603

Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007.

Auteurs : Bin-Yan Wu ; Jian-Ren Ye ; Lin Huang ; Ling-Min He ; De-Wei Li

Source :

Journal of microbiological methods [ 1872-8359 ] ; 2017.

RBID : pubmed:27725176

English descriptors

KwdEn :
- Bacterial Proteins (genetics), Burkholderia (classification), Burkholderia (genetics), Burkholderia (isolation & purification), Genes, Bacterial (MeSH), Real-Time Polymerase Chain Reaction (MeSH).
MESH :
- chemical , genetics : Bacterial Proteins.
- classification : Burkholderia.
- genetics : Burkholderia.
- isolation & purification : Burkholderia.
- Genes, Bacterial, Real-Time Polymerase Chain Reaction.

Abstract

Burkholderia pyrrocinia strain JK-SH007 isolated from poplar stems plays a highly significant role in the growth promotion and the biocontrol of poplar canker during colonization in poplar. In this research, the ideal reference gene was filtered and determined for the transcript normalization. Additionally, the expression of pyrG under all four conditions was relatively stable in B. pyrrocinia JK-SH007.

DOI: 10.1016/j.mimet.2016.10.004
PubMed: 27725176

Links to Exploration step

pubmed:27725176

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007.</title>
<author><name sortKey="Wu, Bin Yan" sort="Wu, Bin Yan" uniqKey="Wu B" first="Bin-Yan" last="Wu">Bin-Yan Wu</name>
<affiliation><nlm:affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Ye, Jian Ren" sort="Ye, Jian Ren" uniqKey="Ye J" first="Jian-Ren" last="Ye">Jian-Ren Ye</name>
<affiliation><nlm:affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China. Electronic address: jrye@njfu.edu.cn.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Huang, Lin" sort="Huang, Lin" uniqKey="Huang L" first="Lin" last="Huang">Lin Huang</name>
<affiliation><nlm:affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="He, Ling Min" sort="He, Ling Min" uniqKey="He L" first="Ling-Min" last="He">Ling-Min He</name>
<affiliation><nlm:affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Li, De Wei" sort="Li, De Wei" uniqKey="Li D" first="De-Wei" last="Li">De-Wei Li</name>
<affiliation><nlm:affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China; The Connecticut Agricultural Experiment Station, Valley Laboratory, 153 Cook Hill Road, Windsor, CT, United States.</nlm:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2017">2017</date>
<idno type="RBID">pubmed:27725176</idno>
<idno type="pmid">27725176</idno>
<idno type="doi">10.1016/j.mimet.2016.10.004</idno>
<idno type="wicri:Area/Main/Corpus">001602</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001602</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007.</title>
<author><name sortKey="Wu, Bin Yan" sort="Wu, Bin Yan" uniqKey="Wu B" first="Bin-Yan" last="Wu">Bin-Yan Wu</name>
<affiliation><nlm:affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Ye, Jian Ren" sort="Ye, Jian Ren" uniqKey="Ye J" first="Jian-Ren" last="Ye">Jian-Ren Ye</name>
<affiliation><nlm:affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China. Electronic address: jrye@njfu.edu.cn.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Huang, Lin" sort="Huang, Lin" uniqKey="Huang L" first="Lin" last="Huang">Lin Huang</name>
<affiliation><nlm:affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="He, Ling Min" sort="He, Ling Min" uniqKey="He L" first="Ling-Min" last="He">Ling-Min He</name>
<affiliation><nlm:affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Li, De Wei" sort="Li, De Wei" uniqKey="Li D" first="De-Wei" last="Li">De-Wei Li</name>
<affiliation><nlm:affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China; The Connecticut Agricultural Experiment Station, Valley Laboratory, 153 Cook Hill Road, Windsor, CT, United States.</nlm:affiliation>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of microbiological methods</title>
<idno type="eISSN">1872-8359</idno>
<imprint><date when="2017" type="published">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (genetics)</term>
<term>Burkholderia (classification)</term>
<term>Burkholderia (genetics)</term>
<term>Burkholderia (isolation & purification)</term>
<term>Genes, Bacterial (MeSH)</term>
<term>Real-Time Polymerase Chain Reaction (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Burkholderia</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Burkholderia</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Burkholderia</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Genes, Bacterial</term>
<term>Real-Time Polymerase Chain Reaction</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Burkholderia pyrrocinia strain JK-SH007 isolated from poplar stems plays a highly significant role in the growth promotion and the biocontrol of poplar canker during colonization in poplar. In this research, the ideal reference gene was filtered and determined for the transcript normalization. Additionally, the expression of pyrG under all four conditions was relatively stable in B. pyrrocinia JK-SH007.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27725176</PMID>
<DateCompleted><Year>2017</Year>
<Month>09</Month>
<Day>11</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>01</Month>
<Day>26</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-8359</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>132</Volume>
<PubDate><Year>2017</Year>
<Month>01</Month>
</PubDate>
</JournalIssue>
<Title>Journal of microbiological methods</Title>
<ISOAbbreviation>J Microbiol Methods</ISOAbbreviation>
</Journal>
<ArticleTitle>Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007.</ArticleTitle>
<Pagination><MedlinePgn>95-98</MedlinePgn>
</Pagination>
<ELocationID EIdType="pii" ValidYN="Y">S0167-7012(16)30291-3</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.mimet.2016.10.004</ELocationID>
<Abstract><AbstractText>Burkholderia pyrrocinia strain JK-SH007 isolated from poplar stems plays a highly significant role in the growth promotion and the biocontrol of poplar canker during colonization in poplar. In this research, the ideal reference gene was filtered and determined for the transcript normalization. Additionally, the expression of pyrG under all four conditions was relatively stable in B. pyrrocinia JK-SH007.</AbstractText>
<CopyrightInformation>Copyright © 2016. Published by Elsevier B.V.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Bin-Yan</ForeName>
<Initials>BY</Initials>
<AffiliationInfo><Affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ye</LastName>
<ForeName>Jian-Ren</ForeName>
<Initials>JR</Initials>
<AffiliationInfo><Affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China. Electronic address: jrye@njfu.edu.cn.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Huang</LastName>
<ForeName>Lin</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>He</LastName>
<ForeName>Ling-Min</ForeName>
<Initials>LM</Initials>
<AffiliationInfo><Affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>De-Wei</ForeName>
<Initials>DW</Initials>
<AffiliationInfo><Affiliation>College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China; The Connecticut Agricultural Experiment Station, Valley Laboratory, 153 Cook Hill Road, Windsor, CT, United States.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>10</Month>
<Day>08</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>J Microbiol Methods</MedlineTA>
<NlmUniqueID>8306883</NlmUniqueID>
<ISSNLinking>0167-7012</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019117" MajorTopicYN="N">Burkholderia</DescriptorName>
<QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005798" MajorTopicYN="Y">Genes, Bacterial</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060888" MajorTopicYN="Y">Real-Time Polymerase Chain Reaction</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Burkholderia pyrrocinia</Keyword>
<Keyword MajorTopicYN="Y">Expression stability</Keyword>
<Keyword MajorTopicYN="Y">RT-qPCR</Keyword>
<Keyword MajorTopicYN="Y">Reference genes</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year>
<Month>07</Month>
<Day>12</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2016</Year>
<Month>10</Month>
<Day>06</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
<Month>10</Month>
<Day>06</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2016</Year>
<Month>10</Month>
<Day>12</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>9</Month>
<Day>12</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2016</Year>
<Month>10</Month>
<Day>12</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">27725176</ArticleId>
<ArticleId IdType="pii">S0167-7012(16)30291-3</ArticleId>
<ArticleId IdType="doi">10.1016/j.mimet.2016.10.004</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001602 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 001602 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PoplarV1
   |flux=    Main
   |étape=   Corpus
   |type=    RBID
   |clé=     pubmed:27725176
   |texte=   Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i   -Sk "pubmed:27725176" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd   \
       | NlmPubMed2Wicri -a PoplarV1

This area was generated with Dilib version V0.6.37.
Data generation: Wed Nov 18 12:07:19 2020. Site generation: Wed Nov 18 12:16:31 2020

	Serveur d'exploration sur le peuplier
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le peuplier

Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007.

Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki