Proteome analysis reveals an energy-dependent central process for Populus×canadensis seed germination.
Identifieur interne : 001369 ( Main/Corpus ); précédent : 001368; suivant : 001370Proteome analysis reveals an energy-dependent central process for Populus×canadensis seed germination.
Auteurs : Hong Zhang ; Ke-Xin Zhou ; Wei-Qing Wang ; Shu-Jun Liu ; Song-Quan SongSource :
- Journal of plant physiology [ 1618-1328 ] ; 2017.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Abscisic Acid, Cycloheximide, Plant Proteins, Proteome.
- metabolism : Populus, Seeds.
- physiology : Germination, Populus, Seeds.
Abstract
Poplar (Populus×canadensis) seeds rapidly germinated in darkness at 10, 15, and 20°C and reached 50% seed germination after about 22, 4.5, and 3.5h, respectively. Germination of poplar seeds was markedly inhibited by abscisic acid (ABA) at 50μM and cycloheximide (CHX) at 100μM, and these inhibitive roles were temperature-dependent. In the present study, mature poplar seeds were used to investigate the differentially changed proteome of seeds germinating in water, ABA, and CHX. A total of 130 protein spots showed a significant change (1.5-fold increase/decrease, P<0.05) in abundance, and 101 protein spots were successfully identified. Most of the proteins were associated with cell defense and rescue (21%), storage proteins (21%), protein synthesis and destination (20%), metabolism (16%), and energy (14%). The germination of poplar seeds is closely related with the increase in those proteins involved in amino acid and lipid metabolism, the tricarboxylic acid cycle and pentose phosphate pathway, protein synthesis and destination, cell defense and rescue, and degradation of storage proteins. ABA and CHX inhibit the germination of poplar seeds by decreasing the protein abundance associated with protein proteolysis, protein folding, and storage proteins. We conclude that poplar seed germination is an energy-dependent active process, and is accompanied by increasing amino acid activation, protein synthesis and destination, as well as cell defense and rescue, and degradation of storage proteins.
DOI: 10.1016/j.jplph.2017.03.008
PubMed: 28384531
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pubmed:28384531Le document en format XML
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<author><name sortKey="Zhang, Hong" sort="Zhang, Hong" uniqKey="Zhang H" first="Hong" last="Zhang">Hong Zhang</name>
<affiliation><nlm:affiliation>Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.</nlm:affiliation>
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<author><name sortKey="Zhou, Ke Xin" sort="Zhou, Ke Xin" uniqKey="Zhou K" first="Ke-Xin" last="Zhou">Ke-Xin Zhou</name>
<affiliation><nlm:affiliation>Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China.</nlm:affiliation>
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<author><name sortKey="Wang, Wei Qing" sort="Wang, Wei Qing" uniqKey="Wang W" first="Wei-Qing" last="Wang">Wei-Qing Wang</name>
<affiliation><nlm:affiliation>Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.</nlm:affiliation>
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<author><name sortKey="Liu, Shu Jun" sort="Liu, Shu Jun" uniqKey="Liu S" first="Shu-Jun" last="Liu">Shu-Jun Liu</name>
<affiliation><nlm:affiliation>Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.</nlm:affiliation>
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<author><name sortKey="Song, Song Quan" sort="Song, Song Quan" uniqKey="Song S" first="Song-Quan" last="Song">Song-Quan Song</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Proteome analysis reveals an energy-dependent central process for Populus×canadensis seed germination.</title>
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<author><name sortKey="Wang, Wei Qing" sort="Wang, Wei Qing" uniqKey="Wang W" first="Wei-Qing" last="Wang">Wei-Qing Wang</name>
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<author><name sortKey="Liu, Shu Jun" sort="Liu, Shu Jun" uniqKey="Liu S" first="Shu-Jun" last="Liu">Shu-Jun Liu</name>
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<term>Plant Proteins (metabolism)</term>
<term>Populus (metabolism)</term>
<term>Populus (physiology)</term>
<term>Proteome (metabolism)</term>
<term>Seeds (metabolism)</term>
<term>Seeds (physiology)</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Abscisic Acid</term>
<term>Cycloheximide</term>
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<front><div type="abstract" xml:lang="en">Poplar (Populus×canadensis) seeds rapidly germinated in darkness at 10, 15, and 20°C and reached 50% seed germination after about 22, 4.5, and 3.5h, respectively. Germination of poplar seeds was markedly inhibited by abscisic acid (ABA) at 50μM and cycloheximide (CHX) at 100μM, and these inhibitive roles were temperature-dependent. In the present study, mature poplar seeds were used to investigate the differentially changed proteome of seeds germinating in water, ABA, and CHX. A total of 130 protein spots showed a significant change (1.5-fold increase/decrease, P<0.05) in abundance, and 101 protein spots were successfully identified. Most of the proteins were associated with cell defense and rescue (21%), storage proteins (21%), protein synthesis and destination (20%), metabolism (16%), and energy (14%). The germination of poplar seeds is closely related with the increase in those proteins involved in amino acid and lipid metabolism, the tricarboxylic acid cycle and pentose phosphate pathway, protein synthesis and destination, cell defense and rescue, and degradation of storage proteins. ABA and CHX inhibit the germination of poplar seeds by decreasing the protein abundance associated with protein proteolysis, protein folding, and storage proteins. We conclude that poplar seed germination is an energy-dependent active process, and is accompanied by increasing amino acid activation, protein synthesis and destination, as well as cell defense and rescue, and degradation of storage proteins.</div>
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<Abstract><AbstractText>Poplar (Populus×canadensis) seeds rapidly germinated in darkness at 10, 15, and 20°C and reached 50% seed germination after about 22, 4.5, and 3.5h, respectively. Germination of poplar seeds was markedly inhibited by abscisic acid (ABA) at 50μM and cycloheximide (CHX) at 100μM, and these inhibitive roles were temperature-dependent. In the present study, mature poplar seeds were used to investigate the differentially changed proteome of seeds germinating in water, ABA, and CHX. A total of 130 protein spots showed a significant change (1.5-fold increase/decrease, P<0.05) in abundance, and 101 protein spots were successfully identified. Most of the proteins were associated with cell defense and rescue (21%), storage proteins (21%), protein synthesis and destination (20%), metabolism (16%), and energy (14%). The germination of poplar seeds is closely related with the increase in those proteins involved in amino acid and lipid metabolism, the tricarboxylic acid cycle and pentose phosphate pathway, protein synthesis and destination, cell defense and rescue, and degradation of storage proteins. ABA and CHX inhibit the germination of poplar seeds by decreasing the protein abundance associated with protein proteolysis, protein folding, and storage proteins. We conclude that poplar seed germination is an energy-dependent active process, and is accompanied by increasing amino acid activation, protein synthesis and destination, as well as cell defense and rescue, and degradation of storage proteins.</AbstractText>
<CopyrightInformation>Copyright © 2017 Elsevier GmbH. All rights reserved.</CopyrightInformation>
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