Transgenic tobacco plants expressing a P1B-ATPase gene from Populus tomentosa Carr. (PtoHMA5) demonstrate improved cadmium transport.
Identifieur interne : 000F41 ( Main/Corpus ); précédent : 000F40; suivant : 000F42Transgenic tobacco plants expressing a P1B-ATPase gene from Populus tomentosa Carr. (PtoHMA5) demonstrate improved cadmium transport.
Auteurs : Xiaotong Wang ; Junkai Zhi ; Xinru Liu ; Hao Zhang ; Huabo Liu ; Jichen XuSource :
- International journal of biological macromolecules [ 1879-0003 ] ; 2018.
English descriptors
- KwdEn :
- Adenosine Triphosphatases (chemistry), Adenosine Triphosphatases (genetics), Adenosine Triphosphatases (metabolism), Amino Acid Sequence (MeSH), Base Sequence (MeSH), Biodegradation, Environmental (MeSH), Biological Transport (MeSH), Cadmium (isolation & purification), Cadmium (metabolism), Gene Expression (MeSH), Plants, Genetically Modified (MeSH), Populus (enzymology), Populus (genetics), Soil Pollutants (isolation & purification), Soil Pollutants (metabolism), Tobacco (genetics), Tobacco (metabolism), Transformation, Genetic (MeSH).
- MESH :
- chemical , chemistry : Adenosine Triphosphatases.
- chemical , genetics : Adenosine Triphosphatases.
- chemical , isolation & purification : Cadmium, Soil Pollutants.
- chemical , metabolism : Adenosine Triphosphatases, Cadmium, Soil Pollutants.
- enzymology : Populus.
- genetics : Populus, Tobacco.
- metabolism : Tobacco.
- Amino Acid Sequence, Base Sequence, Biodegradation, Environmental, Biological Transport, Gene Expression, Plants, Genetically Modified, Transformation, Genetic.
Abstract
Heavy metal ATPase (HMA) plays an important role in phytoremediation via long-distance transportation from root to shoot. In this report, we identified a heavy metal ATPase gene, PtoHMA5, from Populus tomentosa Carr. Its encoded peptide consists of 967 amino acids and has eight trans-membrane motifs inside. Tobacco plants were transformed with this gene via Agrobacterium tumefaciens-mediated method. After exposure to 50mg/LCdCl2 for 10d, the transgenic lines displayed higher cadmium accumulation in leaves than did the wild-type plants with an absolute increase of 25.04%, while the transfer coefficient increased by 16.01%-43.25%. Physiological testing including assessment of relative electrolytic leakage (REL), malondialdehyde (MDA) content, and chlorophyll content revealed that the transgenic lines were seriously affected when compared with the wild-type plants. In summary, PtoHMA5 is really involved in cadmium transport from root to shoot but is not associated with the removal of cadmium toxicity.
DOI: 10.1016/j.ijbiomac.2018.02.081
PubMed: 29501753
Links to Exploration step
pubmed:29501753Le document en format XML
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<term>Base Sequence (MeSH)</term>
<term>Biodegradation, Environmental (MeSH)</term>
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<term>Cadmium (isolation & purification)</term>
<term>Cadmium (metabolism)</term>
<term>Gene Expression (MeSH)</term>
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<term>Soil Pollutants (isolation & purification)</term>
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<front><div type="abstract" xml:lang="en">Heavy metal ATPase (HMA) plays an important role in phytoremediation via long-distance transportation from root to shoot. In this report, we identified a heavy metal ATPase gene, PtoHMA5, from Populus tomentosa Carr. Its encoded peptide consists of 967 amino acids and has eight trans-membrane motifs inside. Tobacco plants were transformed with this gene via Agrobacterium tumefaciens-mediated method. After exposure to 50mg/LCdCl<sub>2</sub>
for 10d, the transgenic lines displayed higher cadmium accumulation in leaves than did the wild-type plants with an absolute increase of 25.04%, while the transfer coefficient increased by 16.01%-43.25%. Physiological testing including assessment of relative electrolytic leakage (REL), malondialdehyde (MDA) content, and chlorophyll content revealed that the transgenic lines were seriously affected when compared with the wild-type plants. In summary, PtoHMA5 is really involved in cadmium transport from root to shoot but is not associated with the removal of cadmium toxicity.</div>
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<ArticleTitle>Transgenic tobacco plants expressing a P1B-ATPase gene from Populus tomentosa Carr. (PtoHMA5) demonstrate improved cadmium transport.</ArticleTitle>
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<Abstract><AbstractText>Heavy metal ATPase (HMA) plays an important role in phytoremediation via long-distance transportation from root to shoot. In this report, we identified a heavy metal ATPase gene, PtoHMA5, from Populus tomentosa Carr. Its encoded peptide consists of 967 amino acids and has eight trans-membrane motifs inside. Tobacco plants were transformed with this gene via Agrobacterium tumefaciens-mediated method. After exposure to 50mg/LCdCl<sub>2</sub>
for 10d, the transgenic lines displayed higher cadmium accumulation in leaves than did the wild-type plants with an absolute increase of 25.04%, while the transfer coefficient increased by 16.01%-43.25%. Physiological testing including assessment of relative electrolytic leakage (REL), malondialdehyde (MDA) content, and chlorophyll content revealed that the transgenic lines were seriously affected when compared with the wild-type plants. In summary, PtoHMA5 is really involved in cadmium transport from root to shoot but is not associated with the removal of cadmium toxicity.</AbstractText>
<CopyrightInformation>Copyright © 2018. Published by Elsevier B.V.</CopyrightInformation>
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