Chromosome painting and comparative physical mapping of the sex chromosomes in Populus tomentosa and Populus deltoides.
Identifieur interne : 000F32 ( Main/Corpus ); précédent : 000F31; suivant : 000F33Chromosome painting and comparative physical mapping of the sex chromosomes in Populus tomentosa and Populus deltoides.
Auteurs : Haoyang Xin ; Tao Zhang ; Yonghua Han ; Yufeng Wu ; Jisen Shi ; Mengli Xi ; Jiming JiangSource :
- Chromosoma [ 1432-0886 ] ; 2018.
English descriptors
- KwdEn :
- MESH :
Abstract
Dioecious species accounted for 6% of all plant species, including a number of crops and economically important species, such as poplar. However, sex determination and sex chromosome evolution have been studied only in few dioecious species. In poplar, the sex-determining locus was mapped to chromosome 19. Interestingly, this locus was mapped to either a peritelomeric or a centromeric region among different poplar species. We developed an oligonucleotide (oligo)-based chromosome painting probe based on the sequence of chromosome 19 from Populus trichocarpa. We performed chromosome painting in P. tomentosa and P. deltoides. Surprisingly, the distal end on the short arm of chromosome 19, which corresponds to the location of the sex-determining locus reported in several species, was not painted in both species. Thus, the DNA sequences associated with this region have not been anchored to the current chromosome 19 pseudomolecule, which was confirmed by painting of somatic metaphase chromosome 19 of P. trichocarpa. Interestingly, the unpainted distal ends of the two chromosome 19 did not pair at the pachytene stage in 22-24% of the meiotic cells in the two species, suggest that these regions from the sex chromosomes have structurally diverged from each other, resulting in the reduced pairing frequency. These results shed light on divergence of a pair of young sex chromosomes in poplar.
DOI: 10.1007/s00412-018-0664-y
PubMed: 29520650
Links to Exploration step
pubmed:29520650Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Chromosome painting and comparative physical mapping of the sex chromosomes in Populus tomentosa and Populus deltoides.</title><author><name sortKey="Xin, Haoyang" sort="Xin, Haoyang" uniqKey="Xin H" first="Haoyang" last="Xin">Haoyang Xin</name><affiliation><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China/Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Tao" sort="Zhang, Tao" uniqKey="Zhang T" first="Tao" last="Zhang">Tao Zhang</name><affiliation><nlm:affiliation>Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Centre for Modern Production Technology of Grain Crops/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, 225009, China.</nlm:affiliation></affiliation></author><author><name sortKey="Han, Yonghua" sort="Han, Yonghua" uniqKey="Han Y" first="Yonghua" last="Han">Yonghua Han</name><affiliation><nlm:affiliation>The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, 221116, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Yufeng" sort="Wu, Yufeng" uniqKey="Wu Y" first="Yufeng" last="Wu">Yufeng Wu</name><affiliation><nlm:affiliation>The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Bioinformatics Center, Nanjing Agricultural University, Nanjing, 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Shi, Jisen" sort="Shi, Jisen" uniqKey="Shi J" first="Jisen" last="Shi">Jisen Shi</name><affiliation><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China/Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xi, Mengli" sort="Xi, Mengli" uniqKey="Xi M" first="Mengli" last="Xi">Mengli Xi</name><affiliation><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China/Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China. ximenglinjfu@126.com.</nlm:affiliation></affiliation></author><author><name sortKey="Jiang, Jiming" sort="Jiang, Jiming" uniqKey="Jiang J" first="Jiming" last="Jiang">Jiming Jiang</name><affiliation><nlm:affiliation>Department of Plant Biology, Department of Horticulture, Michigan State University, East Lansing, MI, 48824, USA.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29520650</idno><idno type="pmid">29520650</idno><idno type="doi">10.1007/s00412-018-0664-y</idno><idno type="wicri:Area/Main/Corpus">000F32</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000F32</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Chromosome painting and comparative physical mapping of the sex chromosomes in Populus tomentosa and Populus deltoides.</title><author><name sortKey="Xin, Haoyang" sort="Xin, Haoyang" uniqKey="Xin H" first="Haoyang" last="Xin">Haoyang Xin</name><affiliation><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China/Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Tao" sort="Zhang, Tao" uniqKey="Zhang T" first="Tao" last="Zhang">Tao Zhang</name><affiliation><nlm:affiliation>Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Centre for Modern Production Technology of Grain Crops/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, 225009, China.</nlm:affiliation></affiliation></author><author><name sortKey="Han, Yonghua" sort="Han, Yonghua" uniqKey="Han Y" first="Yonghua" last="Han">Yonghua Han</name><affiliation><nlm:affiliation>The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, 221116, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Yufeng" sort="Wu, Yufeng" uniqKey="Wu Y" first="Yufeng" last="Wu">Yufeng Wu</name><affiliation><nlm:affiliation>The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Bioinformatics Center, Nanjing Agricultural University, Nanjing, 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Shi, Jisen" sort="Shi, Jisen" uniqKey="Shi J" first="Jisen" last="Shi">Jisen Shi</name><affiliation><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China/Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xi, Mengli" sort="Xi, Mengli" uniqKey="Xi M" first="Mengli" last="Xi">Mengli Xi</name><affiliation><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China/Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China. ximenglinjfu@126.com.</nlm:affiliation></affiliation></author><author><name sortKey="Jiang, Jiming" sort="Jiang, Jiming" uniqKey="Jiang J" first="Jiming" last="Jiang">Jiming Jiang</name><affiliation><nlm:affiliation>Department of Plant Biology, Department of Horticulture, Michigan State University, East Lansing, MI, 48824, USA.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Chromosoma</title><idno type="eISSN">1432-0886</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromosome Painting (MeSH)</term><term>Chromosome Pairing (MeSH)</term><term>Chromosomes, Plant (MeSH)</term><term>In Situ Hybridization, Fluorescence (MeSH)</term><term>Physical Chromosome Mapping (MeSH)</term><term>Populus (genetics)</term><term>Sex Chromosomes (MeSH)</term><term>Species Specificity (MeSH)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromosome Painting</term><term>Chromosome Pairing</term><term>Chromosomes, Plant</term><term>In Situ Hybridization, Fluorescence</term><term>Physical Chromosome Mapping</term><term>Sex Chromosomes</term><term>Species Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Dioecious species accounted for 6% of all plant species, including a number of crops and economically important species, such as poplar. However, sex determination and sex chromosome evolution have been studied only in few dioecious species. In poplar, the sex-determining locus was mapped to chromosome 19. Interestingly, this locus was mapped to either a peritelomeric or a centromeric region among different poplar species. We developed an oligonucleotide (oligo)-based chromosome painting probe based on the sequence of chromosome 19 from Populus trichocarpa. We performed chromosome painting in P. tomentosa and P. deltoides. Surprisingly, the distal end on the short arm of chromosome 19, which corresponds to the location of the sex-determining locus reported in several species, was not painted in both species. Thus, the DNA sequences associated with this region have not been anchored to the current chromosome 19 pseudomolecule, which was confirmed by painting of somatic metaphase chromosome 19 of P. trichocarpa. Interestingly, the unpainted distal ends of the two chromosome 19 did not pair at the pachytene stage in 22-24% of the meiotic cells in the two species, suggest that these regions from the sex chromosomes have structurally diverged from each other, resulting in the reduced pairing frequency. These results shed light on divergence of a pair of young sex chromosomes in poplar.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29520650</PMID><DateCompleted><Year>2019</Year><Month>03</Month><Day>15</Day></DateCompleted><DateRevised><Year>2019</Year><Month>03</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0886</ISSN><JournalIssue CitedMedium="Internet"><Volume>127</Volume><Issue>3</Issue><PubDate><Year>2018</Year><Month>09</Month></PubDate></JournalIssue><Title>Chromosoma</Title><ISOAbbreviation>Chromosoma</ISOAbbreviation></Journal><ArticleTitle>Chromosome painting and comparative physical mapping of the sex chromosomes in Populus tomentosa and Populus deltoides.</ArticleTitle><Pagination><MedlinePgn>313-321</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00412-018-0664-y</ELocationID><Abstract><AbstractText>Dioecious species accounted for 6% of all plant species, including a number of crops and economically important species, such as poplar. However, sex determination and sex chromosome evolution have been studied only in few dioecious species. In poplar, the sex-determining locus was mapped to chromosome 19. Interestingly, this locus was mapped to either a peritelomeric or a centromeric region among different poplar species. We developed an oligonucleotide (oligo)-based chromosome painting probe based on the sequence of chromosome 19 from Populus trichocarpa. We performed chromosome painting in P. tomentosa and P. deltoides. Surprisingly, the distal end on the short arm of chromosome 19, which corresponds to the location of the sex-determining locus reported in several species, was not painted in both species. Thus, the DNA sequences associated with this region have not been anchored to the current chromosome 19 pseudomolecule, which was confirmed by painting of somatic metaphase chromosome 19 of P. trichocarpa. Interestingly, the unpainted distal ends of the two chromosome 19 did not pair at the pachytene stage in 22-24% of the meiotic cells in the two species, suggest that these regions from the sex chromosomes have structurally diverged from each other, resulting in the reduced pairing frequency. These results shed light on divergence of a pair of young sex chromosomes in poplar.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xin</LastName><ForeName>Haoyang</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China/Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Tao</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Centre for Modern Production Technology of Grain Crops/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, 225009, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Yonghua</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, 221116, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Yufeng</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Bioinformatics Center, Nanjing Agricultural University, Nanjing, 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Jisen</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China/Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xi</LastName><ForeName>Mengli</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China/Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China. ximenglinjfu@126.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Jiming</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant Biology, Department of Horticulture, Michigan State University, East Lansing, MI, 48824, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>03</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>Austria</Country><MedlineTA>Chromosoma</MedlineTA><NlmUniqueID>2985138R</NlmUniqueID><ISSNLinking>0009-5915</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020223" MajorTopicYN="Y">Chromosome Painting</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D023902" MajorTopicYN="N">Chromosome Pairing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032461" MajorTopicYN="N">Chromosomes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017404" MajorTopicYN="N">In Situ Hybridization, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020161" MajorTopicYN="Y">Physical Chromosome Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012730" MajorTopicYN="Y">Sex Chromosomes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Chromosome painting</Keyword><Keyword MajorTopicYN="Y">Chromosome pairing</Keyword><Keyword MajorTopicYN="Y">Oligo-FISH</Keyword><Keyword MajorTopicYN="Y">Poplar</Keyword><Keyword MajorTopicYN="Y">Sex chromosome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>10</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>02</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>02</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>3</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29520650</ArticleId><ArticleId IdType="doi">10.1007/s00412-018-0664-y</ArticleId><ArticleId IdType="pii">10.1007/s00412-018-0664-y</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Heredity (Edinb). 2002 Feb;88(2):94-101</Citation><ArticleIdList><ArticleId IdType="pubmed">11932767</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1991 Mar 1;251(4997):1030-3</Citation><ArticleIdList><ArticleId IdType="pubmed">1998119</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome. 2006 Sep;49(9):1057-68</Citation><ArticleIdList><ArticleId IdType="pubmed">17110986</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2008 Jan;53(1):124-32</Citation><ArticleIdList><ArticleId IdType="pubmed">17973896</ArticleId></ArticleIdList></Reference><Reference><Citation>J Struct Biol. 1991 Aug;107(1):6-14</Citation><ArticleIdList><ArticleId IdType="pubmed">1817611</ArticleId></ArticleIdList></Reference><Reference><Citation>Cytogenet Genome Res. 2009;125(1):74-80</Citation><ArticleIdList><ArticleId IdType="pubmed">19617699</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 2015 Jul;24(13):3243-56</Citation><ArticleIdList><ArticleId IdType="pubmed">25728270</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2018 Feb;208(2):513-523</Citation><ArticleIdList><ArticleId IdType="pubmed">29242292</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2016 Aug 18;17:656</Citation><ArticleIdList><ArticleId IdType="pubmed">27538483</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2015 Jul;200(3):771-9</Citation><ArticleIdList><ArticleId IdType="pubmed">25971668</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1998 Mar;13(6):867-76</Citation><ArticleIdList><ArticleId IdType="pubmed">9681023</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2015 Feb;199(2):413-21</Citation><ArticleIdList><ArticleId IdType="pubmed">25480779</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Sep 15;313(5793):1596-604</Citation><ArticleIdList><ArticleId IdType="pubmed">16973872</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann Bot. 2002 Dec;90(6):681-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12451023</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2001 Dec;11(12):2133-41</Citation><ArticleIdList><ArticleId IdType="pubmed">11731505</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2014 Apr;78(1):169-79</Citation><ArticleIdList><ArticleId IdType="pubmed">24635663</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2008 Dec;18(12):1938-43</Citation><ArticleIdList><ArticleId IdType="pubmed">18593814</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biol (Stuttg). 2014 Mar;16(2):411-8</Citation><ArticleIdList><ArticleId IdType="pubmed">23710995</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2012 Aug 21;109(34):13710-5</Citation><ArticleIdList><ArticleId IdType="pubmed">22869747</ArticleId></ArticleIdList></Reference><Reference><Citation>Heredity (Edinb). 2005 Aug;95(2):118-28</Citation><ArticleIdList><ArticleId IdType="pubmed">15931241</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Bot. 2007 Feb;94(2):141-50</Citation><ArticleIdList><ArticleId IdType="pubmed">21642216</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1995 May 9;92(10):4487-91</Citation><ArticleIdList><ArticleId IdType="pubmed">7753830</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2008 Mar;18(3):422-30</Citation><ArticleIdList><ArticleId IdType="pubmed">18256239</ArticleId></ArticleIdList></Reference><Reference><Citation>Genes Genet Syst. 2011;86(4):257-68</Citation><ArticleIdList><ArticleId IdType="pubmed">22214594</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F32 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000F32 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:29520650
|texte= Chromosome painting and comparative physical mapping of the sex chromosomes in Populus tomentosa and Populus deltoides.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:29520650" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |