Abscisic acid promotes root system development in birch tissue culture: a comparison to aspen culture and conventional rooting-related growth regulators.
Identifieur interne : 000B99 ( Main/Corpus ); précédent : 000B98; suivant : 000C00Abscisic acid promotes root system development in birch tissue culture: a comparison to aspen culture and conventional rooting-related growth regulators.
Auteurs : Migl Vai Iukyn ; Jonas Žiauka ; Rasa Ž Kien ; Lidija Vertelkait ; Sigut KuusienSource :
- Physiologia plantarum [ 1399-3054 ] ; 2019.
English descriptors
- KwdEn :
- Abscisic Acid (pharmacology), Betula (cytology), Betula (drug effects), Betula (genetics), Betula (growth & development), Culture Media (chemistry), Culture Media (pharmacology), Genotype (MeSH), Plant Growth Regulators (pharmacology), Plant Roots (drug effects), Plant Roots (growth & development), Plant Shoots (drug effects), Plant Shoots (growth & development), Populus (cytology), Populus (drug effects), Populus (growth & development), Tissue Culture Techniques (methods), Triazoles (pharmacology).
- MESH :
- chemical , chemistry : Culture Media.
- chemical , pharmacology : Abscisic Acid, Culture Media, Plant Growth Regulators, Triazoles.
- cytology : Betula, Populus.
- drug effects : Betula, Plant Roots, Plant Shoots, Populus.
- genetics : Betula.
- growth & development : Betula, Plant Roots, Plant Shoots, Populus.
- methods : Tissue Culture Techniques.
- Genotype.
Abstract
The research aim was to assess the effects of the plant hormone abscisic acid (ABA) and the growth regulator paclobutrazol (PBZ) on root system development during the in vitro culture of different birch and aspen genotypes. The studied genotypes involved two aspen (Populus tremula and Populus tremuloides × P. tremula) and two silver birch (Betula pendula) trees, with one of the birches characterized by its inability to root in vitro. For experiments, apical shoot segments were cultured on nutrient medium enriched with either ABA or PBZ. Additionally, the analysis of the endogenous hormones in shoots developed on hormone-free medium was conducted by high-performance liquid chromatography. The endogenous concentration of auxin indole-3-acetic acid was much higher in the aspens than that in the birches, while the highest concentration of ABA was found in the root-forming birch. The culturing of this birch genotype on medium enriched with ABA resulted in an increased root length and a higher number of lateral roots without any negative effect on either shoot growth or adventitious root (AR) formation, although these two processes were largely inhibited by ABA in the aspens. Meanwhile, PBZ promoted AR formation in both aspen and birch cultures but impaired secondary root formation and shoot growth in birches. These results suggest the use of ABA for the in vitro rooting of birches and PBZ for the rooting of aspens.
DOI: 10.1111/ppl.12860
PubMed: 30367696
Links to Exploration step
pubmed:30367696Le document en format XML
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Kien">Rasa Ž Kien</name><affiliation><nlm:affiliation>Department of Biochemistry, Vytautas Magnus University, Vileikos str. 8, Kaunas, LT-44404, Lithuania.</nlm:affiliation></affiliation></author><author><name sortKey="Vertelkait, Lidija" sort="Vertelkait, Lidija" uniqKey="Vertelkait L" first="Lidija" last="Vertelkait">Lidija Vertelkait</name><affiliation><nlm:affiliation>Lithuanian Research Centre for Agriculture and Forestry, Institute of Forestry, Liepų str. 1, Girionys, Kaunas, LT-53101, Lithuania.</nlm:affiliation></affiliation></author><author><name sortKey="Kuusien, Sigut" sort="Kuusien, Sigut" uniqKey="Kuusien S" first="Sigut" last="Kuusien">Sigut Kuusien</name><affiliation><nlm:affiliation>Lithuanian Research Centre for Agriculture and Forestry, Institute of Forestry, Liepų str. 1, Girionys, Kaunas, LT-53101, Lithuania.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno 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Research Centre for Agriculture and Forestry, Institute of Forestry, Liepų str. 1, Girionys, Kaunas, LT-53101, Lithuania.</nlm:affiliation></affiliation></author><author><name sortKey="Z Kien, Rasa" sort="Z Kien, Rasa" uniqKey="Z Kien R" first="Rasa" last="Ž Kien">Rasa Ž Kien</name><affiliation><nlm:affiliation>Department of Biochemistry, Vytautas Magnus University, Vileikos str. 8, Kaunas, LT-44404, Lithuania.</nlm:affiliation></affiliation></author><author><name sortKey="Vertelkait, Lidija" sort="Vertelkait, Lidija" uniqKey="Vertelkait L" first="Lidija" last="Vertelkait">Lidija Vertelkait</name><affiliation><nlm:affiliation>Lithuanian Research Centre for Agriculture and Forestry, Institute of Forestry, Liepų str. 1, Girionys, Kaunas, LT-53101, Lithuania.</nlm:affiliation></affiliation></author><author><name sortKey="Kuusien, Sigut" sort="Kuusien, Sigut" uniqKey="Kuusien S" first="Sigut" last="Kuusien">Sigut Kuusien</name><affiliation><nlm:affiliation>Lithuanian Research Centre for Agriculture and Forestry, Institute of Forestry, Liepų str. 1, Girionys, Kaunas, LT-53101, Lithuania.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Physiologia plantarum</title><idno type="eISSN">1399-3054</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abscisic Acid (pharmacology)</term><term>Betula (cytology)</term><term>Betula (drug effects)</term><term>Betula (genetics)</term><term>Betula (growth & development)</term><term>Culture Media (chemistry)</term><term>Culture Media (pharmacology)</term><term>Genotype (MeSH)</term><term>Plant Growth Regulators (pharmacology)</term><term>Plant Roots (drug effects)</term><term>Plant Roots (growth & development)</term><term>Plant Shoots (drug effects)</term><term>Plant Shoots (growth & development)</term><term>Populus (cytology)</term><term>Populus (drug effects)</term><term>Populus (growth & development)</term><term>Tissue Culture Techniques (methods)</term><term>Triazoles (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Abscisic Acid</term><term>Culture Media</term><term>Plant Growth Regulators</term><term>Triazoles</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Betula</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Betula</term><term>Plant Roots</term><term>Plant Shoots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Betula</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Betula</term><term>Plant Roots</term><term>Plant Shoots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Tissue Culture Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genotype</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The research aim was to assess the effects of the plant hormone abscisic acid (ABA) and the growth regulator paclobutrazol (PBZ) on root system development during the in vitro culture of different birch and aspen genotypes. The studied genotypes involved two aspen (Populus tremula and Populus tremuloides × P. tremula) and two silver birch (Betula pendula) trees, with one of the birches characterized by its inability to root in vitro. For experiments, apical shoot segments were cultured on nutrient medium enriched with either ABA or PBZ. Additionally, the analysis of the endogenous hormones in shoots developed on hormone-free medium was conducted by high-performance liquid chromatography. The endogenous concentration of auxin indole-3-acetic acid was much higher in the aspens than that in the birches, while the highest concentration of ABA was found in the root-forming birch. The culturing of this birch genotype on medium enriched with ABA resulted in an increased root length and a higher number of lateral roots without any negative effect on either shoot growth or adventitious root (AR) formation, although these two processes were largely inhibited by ABA in the aspens. Meanwhile, PBZ promoted AR formation in both aspen and birch cultures but impaired secondary root formation and shoot growth in birches. These results suggest the use of ABA for the in vitro rooting of birches and PBZ for the rooting of aspens.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30367696</PMID><DateCompleted><Year>2018</Year><Month>12</Month><Day>24</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1399-3054</ISSN><JournalIssue CitedMedium="Internet"><Volume>165</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Physiologia plantarum</Title><ISOAbbreviation>Physiol Plant</ISOAbbreviation></Journal><ArticleTitle>Abscisic acid promotes root system development in birch tissue culture: a comparison to aspen culture and conventional rooting-related growth regulators.</ArticleTitle><Pagination><MedlinePgn>114-122</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/ppl.12860</ELocationID><Abstract><AbstractText>The research aim was to assess the effects of the plant hormone abscisic acid (ABA) and the growth regulator paclobutrazol (PBZ) on root system development during the in vitro culture of different birch and aspen genotypes. The studied genotypes involved two aspen (Populus tremula and Populus tremuloides × P. tremula) and two silver birch (Betula pendula) trees, with one of the birches characterized by its inability to root in vitro. For experiments, apical shoot segments were cultured on nutrient medium enriched with either ABA or PBZ. Additionally, the analysis of the endogenous hormones in shoots developed on hormone-free medium was conducted by high-performance liquid chromatography. The endogenous concentration of auxin indole-3-acetic acid was much higher in the aspens than that in the birches, while the highest concentration of ABA was found in the root-forming birch. The culturing of this birch genotype on medium enriched with ABA resulted in an increased root length and a higher number of lateral roots without any negative effect on either shoot growth or adventitious root (AR) formation, although these two processes were largely inhibited by ABA in the aspens. Meanwhile, PBZ promoted AR formation in both aspen and birch cultures but impaired secondary root formation and shoot growth in birches. These results suggest the use of ABA for the in vitro rooting of birches and PBZ for the rooting of aspens.</AbstractText><CopyrightInformation>© 2018 Scandinavian Plant Physiology Society.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vaičiukynė</LastName><ForeName>Miglė</ForeName><Initials>M</Initials><Identifier Source="ORCID">https://orcid.org/0000-0001-6077-2545</Identifier><AffiliationInfo><Affiliation>Lithuanian Research Centre for Agriculture and Forestry, Institute of Forestry, Liepų str. 1, Girionys, Kaunas, LT-53101, Lithuania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Žiauka</LastName><ForeName>Jonas</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Lithuanian Research Centre for Agriculture and Forestry, Institute of Forestry, Liepų str. 1, Girionys, Kaunas, LT-53101, Lithuania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Žūkienė</LastName><ForeName>Rasa</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Vytautas Magnus University, Vileikos str. 8, Kaunas, LT-44404, Lithuania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vertelkaitė</LastName><ForeName>Lidija</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Lithuanian Research Centre for Agriculture and Forestry, Institute of Forestry, Liepų str. 1, Girionys, Kaunas, LT-53101, Lithuania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kuusienė</LastName><ForeName>Sigutė</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Lithuanian Research Centre for Agriculture and Forestry, Institute of Forestry, Liepų str. 1, Girionys, Kaunas, LT-53101, Lithuania.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>Lithuanian Research Centre for Agriculture and Forestry</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>11</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>Physiol Plant</MedlineTA><NlmUniqueID>1256322</NlmUniqueID><ISSNLinking>0031-9317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010937">Plant Growth Regulators</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014230">Triazoles</NameOfSubstance></Chemical><Chemical><RegistryNumber>6PLV42R3ZA</RegistryNumber><NameOfSubstance UI="C053370">paclobutrazol</NameOfSubstance></Chemical><Chemical><RegistryNumber>72S9A8J5GW</RegistryNumber><NameOfSubstance UI="D000040">Abscisic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000040" MajorTopicYN="N">Abscisic Acid</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029662" MajorTopicYN="N">Betula</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010937" MajorTopicYN="N">Plant Growth Regulators</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018520" MajorTopicYN="N">Plant Shoots</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046509" MajorTopicYN="N">Tissue Culture Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014230" MajorTopicYN="N">Triazoles</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>06</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>10</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>10</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>10</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>12</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>10</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30367696</ArticleId><ArticleId IdType="doi">10.1111/ppl.12860</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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