Overexpression of PtDXS Enhances Stress Resistance in Poplars.
Identifieur interne : 000939 ( Main/Corpus ); précédent : 000938; suivant : 000940Overexpression of PtDXS Enhances Stress Resistance in Poplars.
Auteurs : Hui Wei ; Ali Movahedi ; Chen Xu ; Weibo Sun ; Amir Almasi Zadeh Yaghuti ; Pu Wang ; Dawei Li ; Qiang ZhugeSource :
- International journal of molecular sciences [ 1422-0067 ] ; 2019.
English descriptors
- KwdEn :
- Animals (MeSH), Ascomycota (physiology), Gene Expression Regulation, Plant (drug effects), Genes, Plant (MeSH), Moths (physiology), Organ Specificity (drug effects), Phylogeny (MeSH), Plant Diseases (microbiology), Plant Diseases (parasitology), Plant Growth Regulators (genetics), Plant Growth Regulators (metabolism), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (isolation & purification), Plant Proteins (metabolism), Plants, Genetically Modified (MeSH), Polyethylene Glycols (pharmacology), Populus (drug effects), Populus (genetics), Populus (microbiology), Populus (physiology), Protein Structure, Tertiary (MeSH), Sequence Analysis, Protein (MeSH), Sodium Chloride (pharmacology), Stress, Physiological (drug effects), Stress, Physiological (genetics).
- MESH :
- chemical , chemistry : Plant Proteins.
- chemical , genetics : Plant Growth Regulators, Plant Proteins.
- drug effects : Gene Expression Regulation, Plant, Organ Specificity, Populus, Stress, Physiological.
- genetics : Populus, Stress, Physiological.
- chemical , isolation & purification : Plant Proteins.
- chemical , metabolism : Plant Growth Regulators, Plant Proteins.
- microbiology : Plant Diseases, Populus.
- parasitology : Plant Diseases.
- chemical , pharmacology : Polyethylene Glycols, Sodium Chloride.
- physiology : Ascomycota, Moths, Populus.
- Animals, Genes, Plant, Phylogeny, Plants, Genetically Modified, Protein Structure, Tertiary, Sequence Analysis, Protein.
Abstract
1-Deoxy-d-xylulose-5-phosphate synthase (DXS) is the rate-limiting enzyme in the plastidial methylerythritol phosphate pathway (MEP). In this study, PtDXS (XM_024607716.1) was isolated from Populus trichocarpa. A bioinformatics analysis revealed that PtDXS had high homology with the DXSs of other plant species. PtDXS expression differed among plant tissues and was highest in young leaves and lowest in roots. The recombinant protein was produced in Escherichia coli BL21 (DE3), purified, and its activity evaluated. The purified protein was capable of catalyzing the formation of 1-deoxy-d-xylulose-5-phosphate (DXP) from glyceraldehyde-3-phosphate and pyruvate. A functional color assay in E. coli harboring pAC-BETA indicated that PtDXS encodes a functional protein involved in the biosynthesis of isoprenoid precursors. The treatment of P. trichocarpa seedlings with 200 μM abscisic acid (ABA), 200 mM NaCl, 10% polyethylene glycol6000, and 2 mM H₂O₂ resulted in increased expression of PtDXS. The ABA and gibberellic acid contents of the transgenic lines (Poplar Nanlin 895) were higher than wild types, suggesting that DXS is important in terpenoid biosynthesis. Overexpression of PtDXS enhanced resistance to S. populiperda infection. Furthermore, the transgenic lines showed decreased feeding by Micromelalopha troglodyta, supporting the notion that PtDXS is a key enzyme in terpenoid biosynthesis.
DOI: 10.3390/ijms20071669
PubMed: 30987184
PubMed Central: PMC6479640
Links to Exploration step
pubmed:30987184Le document en format XML
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Amir_20364@yahoo.com.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Pu" sort="Wang, Pu" uniqKey="Wang P" first="Pu" last="Wang">Pu Wang</name><affiliation><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. 18705155218@163.com.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Dawei" sort="Li, Dawei" uniqKey="Li D" first="Dawei" last="Li">Dawei Li</name><affiliation><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. dwli@njfu.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Zhuge, Qiang" sort="Zhuge, Qiang" uniqKey="Zhuge Q" first="Qiang" last="Zhuge">Qiang Zhuge</name><affiliation><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. qzhuge@njfu.edu.cn.</nlm:affiliation></affiliation></author></analytic><series><title level="j">International journal of molecular sciences</title><idno type="eISSN">1422-0067</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Ascomycota (physiology)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Genes, Plant (MeSH)</term><term>Moths (physiology)</term><term>Organ Specificity (drug effects)</term><term>Phylogeny (MeSH)</term><term>Plant Diseases (microbiology)</term><term>Plant Diseases (parasitology)</term><term>Plant Growth Regulators (genetics)</term><term>Plant Growth Regulators (metabolism)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (isolation & purification)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Polyethylene Glycols (pharmacology)</term><term>Populus (drug effects)</term><term>Populus (genetics)</term><term>Populus (microbiology)</term><term>Populus (physiology)</term><term>Protein Structure, Tertiary (MeSH)</term><term>Sequence Analysis, Protein (MeSH)</term><term>Sodium Chloride (pharmacology)</term><term>Stress, Physiological (drug effects)</term><term>Stress, Physiological (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Growth Regulators</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Organ Specificity</term><term>Populus</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Growth Regulators</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Polyethylene Glycols</term><term>Sodium Chloride</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Ascomycota</term><term>Moths</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Genes, Plant</term><term>Phylogeny</term><term>Plants, Genetically Modified</term><term>Protein Structure, Tertiary</term><term>Sequence Analysis, Protein</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">1-Deoxy-d-xylulose-5-phosphate synthase (DXS) is the rate-limiting enzyme in the plastidial methylerythritol phosphate pathway (MEP). In this study, <i>PtDXS</i> (XM_024607716.1) was isolated from <i>Populus trichocarpa</i>. A bioinformatics analysis revealed that <i>PtDXS</i> had high homology with the <i>DXS</i>s of other plant species. <i>PtDXS</i> expression differed among plant tissues and was highest in young leaves and lowest in roots. The recombinant protein was produced in <i>Escherichia coli</i> BL21 (DE3), purified, and its activity evaluated. The purified protein was capable of catalyzing the formation of 1-deoxy-d-xylulose-5-phosphate (DXP) from glyceraldehyde-3-phosphate and pyruvate. A functional color assay in <i>E</i>. <i>coli</i> harboring pAC-BETA indicated that <i>PtDXS</i> encodes a functional protein involved in the biosynthesis of isoprenoid precursors. The treatment of <i>P</i>. <i>trichocarpa</i> seedlings with 200 μM abscisic acid (ABA), 200 mM NaCl, 10% polyethylene glycol<sub>6000</sub>, and 2 mM H₂O₂ resulted in increased expression of <i>PtDXS</i>. The ABA and gibberellic acid contents of the transgenic lines (Poplar Nanlin 895) were higher than wild types, suggesting that DXS is important in terpenoid biosynthesis. Overexpression of <i>PtDXS</i> enhanced resistance to <i>S</i>. <i>populiperda</i> infection. Furthermore, the transgenic lines showed decreased feeding by <i>Micromelalopha troglodyta</i>, supporting the notion that PtDXS is a key enzyme in terpenoid biosynthesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30987184</PMID><DateCompleted><Year>2019</Year><Month>07</Month><Day>30</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1422-0067</ISSN><JournalIssue CitedMedium="Internet"><Volume>20</Volume><Issue>7</Issue><PubDate><Year>2019</Year><Month>Apr</Month><Day>03</Day></PubDate></JournalIssue><Title>International journal of molecular sciences</Title><ISOAbbreviation>Int J Mol Sci</ISOAbbreviation></Journal><ArticleTitle>Overexpression of <i>PtDXS</i> Enhances Stress Resistance in Poplars.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E1669</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/ijms20071669</ELocationID><Abstract><AbstractText>1-Deoxy-d-xylulose-5-phosphate synthase (DXS) is the rate-limiting enzyme in the plastidial methylerythritol phosphate pathway (MEP). In this study, <i>PtDXS</i> (XM_024607716.1) was isolated from <i>Populus trichocarpa</i>. A bioinformatics analysis revealed that <i>PtDXS</i> had high homology with the <i>DXS</i>s of other plant species. <i>PtDXS</i> expression differed among plant tissues and was highest in young leaves and lowest in roots. The recombinant protein was produced in <i>Escherichia coli</i> BL21 (DE3), purified, and its activity evaluated. The purified protein was capable of catalyzing the formation of 1-deoxy-d-xylulose-5-phosphate (DXP) from glyceraldehyde-3-phosphate and pyruvate. A functional color assay in <i>E</i>. <i>coli</i> harboring pAC-BETA indicated that <i>PtDXS</i> encodes a functional protein involved in the biosynthesis of isoprenoid precursors. The treatment of <i>P</i>. <i>trichocarpa</i> seedlings with 200 μM abscisic acid (ABA), 200 mM NaCl, 10% polyethylene glycol<sub>6000</sub>, and 2 mM H₂O₂ resulted in increased expression of <i>PtDXS</i>. The ABA and gibberellic acid contents of the transgenic lines (Poplar Nanlin 895) were higher than wild types, suggesting that DXS is important in terpenoid biosynthesis. Overexpression of <i>PtDXS</i> enhanced resistance to <i>S</i>. <i>populiperda</i> infection. Furthermore, the transgenic lines showed decreased feeding by <i>Micromelalopha troglodyta</i>, supporting the notion that PtDXS is a key enzyme in terpenoid biosynthesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Hui</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. 15850682752@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Movahedi</LastName><ForeName>Ali</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. ali_movahedi@njfu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Chen</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. xuchenidea@hotmail.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Jiangsu Provincial Key Construction Laboratory of Special Biomass Resource Utilization, Nanjing Xiaozhuang University, Nanjing 211171, China. xuchenidea@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Weibo</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. czswb@njfu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Almasi Zadeh Yaghuti</LastName><ForeName>Amir</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. Amir_20364@yahoo.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Pu</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. 18705155218@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Dawei</ForeName><Initials>D</Initials><Identifier Source="ORCID">0000-0002-0840-0602</Identifier><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. dwli@njfu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhuge</LastName><ForeName>Qiang</ForeName><Initials>Q</Initials><Identifier Source="ORCID">0000-0002-9450-487X</Identifier><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. qzhuge@njfu.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>04</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Int J Mol Sci</MedlineTA><NlmUniqueID>101092791</NlmUniqueID><ISSNLinking>1422-0067</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010937">Plant Growth 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MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009036" MajorTopicYN="N">Moths</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009928" MajorTopicYN="N">Organ Specificity</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010937" MajorTopicYN="N">Plant Growth Regulators</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011092" MajorTopicYN="N">Polyethylene Glycols</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName 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