Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar.
Identifieur interne : 000922 ( Main/Corpus ); précédent : 000921; suivant : 000923Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar.
Auteurs : Chunpu Qu ; Bingqing Hao ; Xiuyue Xu ; Yuchen Wang ; Chengjun Yang ; Zhiru Xu ; Guanjun LiuSource :
- Genes [ 2073-4425 ] ; 2019.
English descriptors
- KwdEn :
- Asparagine (metabolism), Aspartate-Ammonia Ligase (chemistry), Aspartate-Ammonia Ligase (genetics), Escherichia coli (genetics), Gene Expression Regulation, Plant (MeSH), Glutamate-Ammonia Ligase (chemistry), Glutamate-Ammonia Ligase (genetics), Glutamine (metabolism), Nitrogen (metabolism), Populus (enzymology), Populus (genetics).
- MESH :
- chemical , chemistry : Aspartate-Ammonia Ligase, Glutamate-Ammonia Ligase.
- chemical , genetics : Aspartate-Ammonia Ligase, Glutamate-Ammonia Ligase.
- chemical , metabolism : Asparagine, Glutamine, Nitrogen.
- enzymology : Populus.
- genetics : Escherichia coli, Populus.
- Gene Expression Regulation, Plant.
Abstract
Asparagine synthetase (AS), a key enzyme in plant nitrogen metabolism, plays an important role in plant nitrogen assimilation and distribution. Asparagine (Asn), the product of asparagine synthetase, is one of the main compounds responsible for organic nitrogen transport and storage in plants. In this study, we performed complementation experiments using an Asn-deficient Escherichia coli strain to demonstrate that three putative asparagine synthetase family members in poplar (Populussimonii× P.nigra) function in Asn synthesis. Quantitative real-time PCR revealed that the three members had high expression levels in different tissues of poplar and were regulated by exogenous nitrogen. PnAS1 and PnAS2 were also affected by diurnal rhythm. Long-term dark treatment resulted in a significant increase in PnAS1 and PnAS3 expression levels. Under long-term light conditions, however, PnAS2 expression decreased significantly in the intermediate region of leaves. Exogenous application of ammonium nitrogen, glutamine, and a glutamine synthetase inhibitor revealed that PnAS3 was more sensitive to exogenous glutamine, while PnAS1 and PnAS2 were more susceptible to exogenous ammonium nitrogen. Our results suggest that the various members of the PnAS gene family have distinct roles in different tissues and are regulated in different ways.
DOI: 10.3390/genes10050326
PubMed: 31035411
PubMed Central: PMC6562506
Links to Exploration step
pubmed:31035411Le document en format XML
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bqhao2019@163.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. bqhao2019@163.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Guangxi Forestry Research Institute, Nanning 530000, China. bqhao2019@163.com.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Xiuyue" sort="Xu, Xiuyue" uniqKey="Xu X" first="Xiuyue" last="Xu">Xiuyue Xu</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China. 15663593162@163.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. 15663593162@163.com.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Yuchen" sort="Wang, Yuchen" uniqKey="Wang Y" first="Yuchen" last="Wang">Yuchen 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Hao</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China. bqhao2019@163.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. bqhao2019@163.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Guangxi Forestry Research Institute, Nanning 530000, China. bqhao2019@163.com.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Xiuyue" sort="Xu, Xiuyue" uniqKey="Xu X" first="Xiuyue" last="Xu">Xiuyue Xu</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China. 15663593162@163.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. 15663593162@163.com.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Yuchen" sort="Wang, Yuchen" uniqKey="Wang Y" first="Yuchen" last="Wang">Yuchen Wang</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China. abc1032788957@126.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. abc1032788957@126.com.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Chengjun" sort="Yang, Chengjun" uniqKey="Yang C" first="Chengjun" last="Yang">Chengjun Yang</name><affiliation><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. nxyycj@sina.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Zhiru" sort="Xu, Zhiru" uniqKey="Xu Z" first="Zhiru" last="Xu">Zhiru Xu</name><affiliation><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. xuzhiru2003@126.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Life Science, Northeast Forestry University, Harbin 150040, China. xuzhiru2003@126.com.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Guanjun" sort="Liu, Guanjun" uniqKey="Liu G" first="Guanjun" last="Liu">Guanjun Liu</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China. liuguanjun2003@126.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. liuguanjun2003@126.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Genes</title><idno type="ISSN">2073-4425</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Asparagine (metabolism)</term><term>Aspartate-Ammonia Ligase (chemistry)</term><term>Aspartate-Ammonia Ligase (genetics)</term><term>Escherichia coli (genetics)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Glutamate-Ammonia Ligase (chemistry)</term><term>Glutamate-Ammonia Ligase (genetics)</term><term>Glutamine (metabolism)</term><term>Nitrogen (metabolism)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Aspartate-Ammonia Ligase</term><term>Glutamate-Ammonia Ligase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Aspartate-Ammonia Ligase</term><term>Glutamate-Ammonia Ligase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Asparagine</term><term>Glutamine</term><term>Nitrogen</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Asparagine synthetase (AS), a key enzyme in plant nitrogen metabolism, plays an important role in plant nitrogen assimilation and distribution. Asparagine (Asn), the product of asparagine synthetase, is one of the main compounds responsible for organic nitrogen transport and storage in plants. In this study, we performed complementation experiments using an Asn-deficient <i>Escherichia coli</i> strain to demonstrate that three putative asparagine synthetase family members in poplar (<i>Populus</i><i>simonii</i><i>× P.</i><i>nigra</i>) function in Asn synthesis. Quantitative real-time PCR revealed that the three members had high expression levels in different tissues of poplar and were regulated by exogenous nitrogen. <i>PnAS1</i> and <i>PnAS2</i> were also affected by diurnal rhythm. Long-term dark treatment resulted in a significant increase in <i>PnAS1</i> and <i>PnAS3</i> expression levels. Under long-term light conditions, however, <i>PnAS2</i> expression decreased significantly in the intermediate region of leaves. Exogenous application of ammonium nitrogen, glutamine, and a glutamine synthetase inhibitor revealed that <i>PnAS3</i> was more sensitive to exogenous glutamine, while <i>PnAS1</i> and <i>PnAS2</i> were more susceptible to exogenous ammonium nitrogen. Our results suggest that the various members of the <i>PnAS</i> gene family have distinct roles in different tissues and are regulated in different ways.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31035411</PMID><DateCompleted><Year>2019</Year><Month>12</Month><Day>26</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>09</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2073-4425</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><Issue>5</Issue><PubDate><Year>2019</Year><Month>04</Month><Day>28</Day></PubDate></JournalIssue><Title>Genes</Title><ISOAbbreviation>Genes (Basel)</ISOAbbreviation></Journal><ArticleTitle>Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E326</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/genes10050326</ELocationID><Abstract><AbstractText>Asparagine synthetase (AS), a key enzyme in plant nitrogen metabolism, plays an important role in plant nitrogen assimilation and distribution. Asparagine (Asn), the product of asparagine synthetase, is one of the main compounds responsible for organic nitrogen transport and storage in plants. In this study, we performed complementation experiments using an Asn-deficient <i>Escherichia coli</i> strain to demonstrate that three putative asparagine synthetase family members in poplar (<i>Populus</i><i>simonii</i><i>× P.</i><i>nigra</i>) function in Asn synthesis. Quantitative real-time PCR revealed that the three members had high expression levels in different tissues of poplar and were regulated by exogenous nitrogen. <i>PnAS1</i> and <i>PnAS2</i> were also affected by diurnal rhythm. Long-term dark treatment resulted in a significant increase in <i>PnAS1</i> and <i>PnAS3</i> expression levels. Under long-term light conditions, however, <i>PnAS2</i> expression decreased significantly in the intermediate region of leaves. Exogenous application of ammonium nitrogen, glutamine, and a glutamine synthetase inhibitor revealed that <i>PnAS3</i> was more sensitive to exogenous glutamine, while <i>PnAS1</i> and <i>PnAS2</i> were more susceptible to exogenous ammonium nitrogen. Our results suggest that the various members of the <i>PnAS</i> gene family have distinct roles in different tissues and are regulated in different ways.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Qu</LastName><ForeName>Chunpu</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China. qcp_0451@163.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. qcp_0451@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hao</LastName><ForeName>Bingqing</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China. bqhao2019@163.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. bqhao2019@163.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Guangxi Forestry Research Institute, Nanning 530000, China. bqhao2019@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Xiuyue</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China. 15663593162@163.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. 15663593162@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yuchen</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China. abc1032788957@126.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. abc1032788957@126.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Chengjun</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. nxyycj@sina.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Zhiru</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. xuzhiru2003@126.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Life Science, Northeast Forestry University, Harbin 150040, China. xuzhiru2003@126.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Guanjun</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China. liuguanjun2003@126.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, Harbin 150040, China. liuguanjun2003@126.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>04</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Genes (Basel)</MedlineTA><NlmUniqueID>101551097</NlmUniqueID><ISSNLinking>2073-4425</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0RH81L854J</RegistryNumber><NameOfSubstance UI="D005973">Glutamine</NameOfSubstance></Chemical><Chemical><RegistryNumber>7006-34-0</RegistryNumber><NameOfSubstance UI="D001216">Asparagine</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 6.3.1.1</RegistryNumber><NameOfSubstance UI="D001217">Aspartate-Ammonia Ligase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 6.3.1.2</RegistryNumber><NameOfSubstance UI="D005974">Glutamate-Ammonia Ligase</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001216" MajorTopicYN="N">Asparagine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001217" MajorTopicYN="N">Aspartate-Ammonia Ligase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005974" MajorTopicYN="N">Glutamate-Ammonia Ligase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005973" MajorTopicYN="N">Glutamine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Poplar</Keyword><Keyword MajorTopicYN="Y">asparagine synthetase</Keyword><Keyword MajorTopicYN="Y">expression patterns</Keyword><Keyword MajorTopicYN="Y">functional research</Keyword><Keyword MajorTopicYN="Y">transcriptional regulation</Keyword></KeywordList><CoiStatement>The authors declare no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>03</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>04</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>04</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>5</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>5</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>5</Month><Day>1</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31035411</ArticleId><ArticleId IdType="pii">genes10050326</ArticleId><ArticleId IdType="doi">10.3390/genes10050326</ArticleId><ArticleId IdType="pmc">PMC6562506</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Tree Physiol. 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