Production of cellulosic butyrate and 3-hydroxybutyrate in engineered Escherichia coli.
Identifieur interne : 000917 ( Main/Corpus ); précédent : 000916; suivant : 000918Production of cellulosic butyrate and 3-hydroxybutyrate in engineered Escherichia coli.
Auteurs : Dragan Miscevic ; Kajan Srirangan ; Teshager Kefale ; Daryoush Abedi ; Murray Moo-Young ; C Perry ChouSource :
- Applied microbiology and biotechnology [ 1432-0614 ] ; 2019.
English descriptors
- KwdEn :
- 3-Hydroxybutyric Acid (biosynthesis), Biomass (MeSH), Biotransformation (MeSH), Butyrates (metabolism), Escherichia coli (genetics), Escherichia coli (metabolism), Ethanol (MeSH), Fermentation (MeSH), Glucose (MeSH), Lignin (metabolism), Metabolic Engineering (methods), Metabolic Networks and Pathways (MeSH), Populus (MeSH), Xylose (MeSH).
- MESH :
- chemical , biosynthesis : 3-Hydroxybutyric Acid.
- chemical , metabolism : Butyrates, Lignin.
- genetics : Escherichia coli.
- metabolism : Escherichia coli.
- methods : Metabolic Engineering.
- Biomass, Biotransformation, Ethanol, Fermentation, Glucose, Metabolic Networks and Pathways, Populus, Xylose.
Abstract
Being the most abundant renewable organic substance on Earth, lignocellulosic biomass has acted as an attractive and cost-effective feedstock for biobased production of value-added products. However, lignocellulosic biomass should be properly treated for its effective utilization during biotransformation. The current work aimed to demonstrate biobased production of butyrate and 3-hydroxybutyrate (3-HB) in engineered Escherichia coli using pretreated and detoxified aspen tree (Populus tremuloides) wood chips as the feedstock. Various bioprocessing and genetic/metabolic factors limiting the production of cellulosic butyrate and 3-HB were identified. With these developed bioprocessing strategies and strain engineering approaches, major carbons in the hydrolysate, including glucose, xylose, and even acetate, could be completely dissimilated during shake-flask cultivation with up to 1.68 g L-1 butyrate, 8.95 g L-1 3-HB, and minimal side metabolites (i.e., acetate and ethanol) being obtained. Our results highlight the importance of consolidating bioprocess and genetic engineering strategies for effective biobased production from lignocellulosic biomass.
DOI: 10.1007/s00253-019-09815-x
PubMed: 31049621
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pubmed:31049621Le document en format XML
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Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Abedi, Daryoush" sort="Abedi, Daryoush" uniqKey="Abedi D" first="Daryoush" last="Abedi">Daryoush Abedi</name><affiliation><nlm:affiliation>Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Drug & Food Control, Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation></affiliation></author><author><name sortKey="Moo Young, Murray" sort="Moo Young, Murray" uniqKey="Moo Young M" first="Murray" last="Moo-Young">Murray Moo-Young</name><affiliation><nlm:affiliation>Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Chou, C Perry" sort="Chou, C Perry" uniqKey="Chou C" first="C Perry" last="Chou">C Perry Chou</name><affiliation><nlm:affiliation>Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada. cpchou@uwaterloo.ca.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31049621</idno><idno type="pmid">31049621</idno><idno type="doi">10.1007/s00253-019-09815-x</idno><idno type="wicri:Area/Main/Corpus">000917</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000917</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Production of cellulosic butyrate and 3-hydroxybutyrate in engineered Escherichia coli.</title><author><name sortKey="Miscevic, Dragan" sort="Miscevic, Dragan" uniqKey="Miscevic D" first="Dragan" last="Miscevic">Dragan Miscevic</name><affiliation><nlm:affiliation>Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Srirangan, Kajan" sort="Srirangan, Kajan" uniqKey="Srirangan K" first="Kajan" last="Srirangan">Kajan Srirangan</name><affiliation><nlm:affiliation>Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, H4P 2R2, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Kefale, Teshager" sort="Kefale, Teshager" uniqKey="Kefale T" first="Teshager" last="Kefale">Teshager Kefale</name><affiliation><nlm:affiliation>Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Abedi, Daryoush" sort="Abedi, Daryoush" uniqKey="Abedi D" first="Daryoush" last="Abedi">Daryoush Abedi</name><affiliation><nlm:affiliation>Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Drug & Food Control, Tehran University of Medical Sciences, Tehran, Iran.</nlm:affiliation></affiliation></author><author><name sortKey="Moo Young, Murray" sort="Moo Young, Murray" uniqKey="Moo Young M" first="Murray" last="Moo-Young">Murray Moo-Young</name><affiliation><nlm:affiliation>Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Chou, C Perry" sort="Chou, C Perry" uniqKey="Chou C" first="C Perry" last="Chou">C Perry Chou</name><affiliation><nlm:affiliation>Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada. cpchou@uwaterloo.ca.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Applied microbiology and biotechnology</title><idno type="eISSN">1432-0614</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3-Hydroxybutyric Acid (biosynthesis)</term><term>Biomass (MeSH)</term><term>Biotransformation (MeSH)</term><term>Butyrates (metabolism)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Ethanol (MeSH)</term><term>Fermentation (MeSH)</term><term>Glucose (MeSH)</term><term>Lignin (metabolism)</term><term>Metabolic Engineering (methods)</term><term>Metabolic Networks and Pathways (MeSH)</term><term>Populus (MeSH)</term><term>Xylose (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>3-Hydroxybutyric Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Butyrates</term><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Metabolic Engineering</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>Biotransformation</term><term>Ethanol</term><term>Fermentation</term><term>Glucose</term><term>Metabolic Networks and Pathways</term><term>Populus</term><term>Xylose</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Being the most abundant renewable organic substance on Earth, lignocellulosic biomass has acted as an attractive and cost-effective feedstock for biobased production of value-added products. However, lignocellulosic biomass should be properly treated for its effective utilization during biotransformation. The current work aimed to demonstrate biobased production of butyrate and 3-hydroxybutyrate (3-HB) in engineered Escherichia coli using pretreated and detoxified aspen tree (Populus tremuloides) wood chips as the feedstock. Various bioprocessing and genetic/metabolic factors limiting the production of cellulosic butyrate and 3-HB were identified. With these developed bioprocessing strategies and strain engineering approaches, major carbons in the hydrolysate, including glucose, xylose, and even acetate, could be completely dissimilated during shake-flask cultivation with up to 1.68 g L<sup>-1</sup> butyrate, 8.95 g L<sup>-1</sup> 3-HB, and minimal side metabolites (i.e., acetate and ethanol) being obtained. Our results highlight the importance of consolidating bioprocess and genetic engineering strategies for effective biobased production from lignocellulosic biomass.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31049621</PMID><DateCompleted><Year>2019</Year><Month>10</Month><Day>31</Day></DateCompleted><DateRevised><Year>2019</Year><Month>10</Month><Day>31</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0614</ISSN><JournalIssue CitedMedium="Internet"><Volume>103</Volume><Issue>13</Issue><PubDate><Year>2019</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Production of cellulosic butyrate and 3-hydroxybutyrate in engineered Escherichia coli.</ArticleTitle><Pagination><MedlinePgn>5215-5230</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00253-019-09815-x</ELocationID><Abstract><AbstractText>Being the most abundant renewable organic substance on Earth, lignocellulosic biomass has acted as an attractive and cost-effective feedstock for biobased production of value-added products. However, lignocellulosic biomass should be properly treated for its effective utilization during biotransformation. The current work aimed to demonstrate biobased production of butyrate and 3-hydroxybutyrate (3-HB) in engineered Escherichia coli using pretreated and detoxified aspen tree (Populus tremuloides) wood chips as the feedstock. Various bioprocessing and genetic/metabolic factors limiting the production of cellulosic butyrate and 3-HB were identified. With these developed bioprocessing strategies and strain engineering approaches, major carbons in the hydrolysate, including glucose, xylose, and even acetate, could be completely dissimilated during shake-flask cultivation with up to 1.68 g L<sup>-1</sup> butyrate, 8.95 g L<sup>-1</sup> 3-HB, and minimal side metabolites (i.e., acetate and ethanol) being obtained. Our results highlight the importance of consolidating bioprocess and genetic engineering strategies for effective biobased production from lignocellulosic biomass.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Miscevic</LastName><ForeName>Dragan</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Srirangan</LastName><ForeName>Kajan</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, H4P 2R2, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kefale</LastName><ForeName>Teshager</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Abedi</LastName><ForeName>Daryoush</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Drug & Food Control, Tehran University of Medical Sciences, Tehran, Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moo-Young</LastName><ForeName>Murray</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chou</LastName><ForeName>C Perry</ForeName><Initials>CP</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada. cpchou@uwaterloo.ca.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>CRC 950-211471</GrantID><Agency>Natural Sciences and Engineering Research Council of Canada</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>05</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002087">Butyrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>11132-73-3</RegistryNumber><NameOfSubstance UI="C036909">lignocellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>A1TA934AKO</RegistryNumber><NameOfSubstance UI="D014994">Xylose</NameOfSubstance></Chemical><Chemical><RegistryNumber>IY9XDZ35W2</RegistryNumber><NameOfSubstance UI="D005947">Glucose</NameOfSubstance></Chemical><Chemical><RegistryNumber>TZP1275679</RegistryNumber><NameOfSubstance UI="D020155">3-Hydroxybutyric Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020155" MajorTopicYN="N">3-Hydroxybutyric Acid</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001711" MajorTopicYN="N">Biotransformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002087" MajorTopicYN="N">Butyrates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000431" MajorTopicYN="N">Ethanol</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="N">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060847" MajorTopicYN="N">Metabolic Engineering</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053858" MajorTopicYN="N">Metabolic Networks and Pathways</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014994" MajorTopicYN="N">Xylose</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">3-Hydroxybutyrate</Keyword><Keyword MajorTopicYN="N">Butyrate</Keyword><Keyword MajorTopicYN="N">Detoxification</Keyword><Keyword MajorTopicYN="N">Escherichia coli</Keyword><Keyword MajorTopicYN="N">Lignocellulosic hydrolysate</Keyword><Keyword MajorTopicYN="N">Over-liming</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>11</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>03</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>03</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>5</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>11</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>5</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31049621</ArticleId><ArticleId IdType="doi">10.1007/s00253-019-09815-x</ArticleId><ArticleId IdType="pii">10.1007/s00253-019-09815-x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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