Significant influence of lignin on axial elastic modulus of poplar wood at low microfibril angles under wet conditions.
Identifieur interne : 000845 ( Main/Corpus ); précédent : 000844; suivant : 000846Significant influence of lignin on axial elastic modulus of poplar wood at low microfibril angles under wet conditions.
Auteurs : Merve Özparpucu ; Notburga Gierlinger ; Igor Cesarino ; Ingo Burgert ; Wout Boerjan ; Markus RüggebergSource :
- Journal of experimental botany [ 1460-2431 ] ; 2019.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Lignin, Plant Proteins.
- genetics : Plants, Genetically Modified, Populus.
- metabolism : Microfibrils, Plants, Genetically Modified, Populus.
- physiology : Elastic Modulus.
Abstract
Wood is extensively used as a construction material. Despite increasing knowledge of its mechanical properties, the contribution of the cell-wall matrix polymers to wood mechanics is still not well understood. Previous studies have shown that axial stiffness correlates with lignin content only for cellulose microfibril angles larger than around 20°, while no influence is found for smaller angles. Here, by analysing the wood of poplar with reduced lignin content due to down-regulation of CAFFEOYL SHIKIMATE ESTERASE, we show that lignin content also influences axial stiffness at smaller angles. Micro-tensile tests of the xylem revealed that axial stiffness was strongly reduced in the low-lignin transgenic lines. Strikingly, microfibril angles were around 15° for both wild-type and transgenic poplars, suggesting that cellulose orientation is not responsible for the observed changes in mechanical behavior. Multiple linear regression analysis showed that the decrease in stiffness was almost completely related to the variation in both density and lignin content. We suggest that the influence of lignin content on axial stiffness may gradually increase as a function of the microfibril angle. Our results may help in building up comprehensive models of the cell wall that can unravel the individual roles of the matrix polymers.
DOI: 10.1093/jxb/erz180
PubMed: 31187131
PubMed Central: PMC6685656
Links to Exploration step
pubmed:31187131Le document en format XML
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SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Burgert, Ingo" sort="Burgert, Ingo" uniqKey="Burgert I" first="Ingo" last="Burgert">Ingo Burgert</name><affiliation><nlm:affiliation>Institute for Building Materials (IfB), ETH Zurich, Zurich, Switzerland.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Cellulose and Wood Materials, EMPA, Dübendorf, Switzerland.</nlm:affiliation></affiliation></author><author><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name><affiliation><nlm:affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>VIB Center for Plant Systems Biology, Ghent, Belgium.</nlm:affiliation></affiliation></author><author><name sortKey="Ruggeberg, Markus" sort="Ruggeberg, Markus" uniqKey="Ruggeberg M" first="Markus" last="Rüggeberg">Markus Rüggeberg</name><affiliation><nlm:affiliation>Institute for Building Materials (IfB), ETH Zurich, Zurich, Switzerland.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Cellulose and Wood Materials, EMPA, Dübendorf, Switzerland.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31187131</idno><idno type="pmid">31187131</idno><idno type="doi">10.1093/jxb/erz180</idno><idno type="pmc">PMC6685656</idno><idno type="wicri:Area/Main/Corpus">000845</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000845</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Significant influence of lignin on axial elastic modulus of poplar wood at low microfibril angles under wet conditions.</title><author><name sortKey="Ozparpucu, Merve" sort="Ozparpucu, Merve" uniqKey="Ozparpucu M" first="Merve" last="Özparpucu">Merve Özparpucu</name><affiliation><nlm:affiliation>Institute for Building Materials (IfB), ETH Zurich, Zurich, Switzerland.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Life Sciences Weihenstephan, Wood Research Munich, Technical University of Munich (TUM), Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Gierlinger, Notburga" sort="Gierlinger, Notburga" uniqKey="Gierlinger N" first="Notburga" last="Gierlinger">Notburga Gierlinger</name><affiliation><nlm:affiliation>Institute for Biophysics, University of Natural Resources and Life Sciences Vienna (BOKU), Wien, Austria.</nlm:affiliation></affiliation></author><author><name sortKey="Cesarino, Igor" sort="Cesarino, Igor" uniqKey="Cesarino I" first="Igor" last="Cesarino">Igor Cesarino</name><affiliation><nlm:affiliation>Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo - SP, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Burgert, Ingo" sort="Burgert, Ingo" uniqKey="Burgert I" first="Ingo" last="Burgert">Ingo Burgert</name><affiliation><nlm:affiliation>Institute for Building Materials (IfB), ETH Zurich, Zurich, Switzerland.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Cellulose and Wood Materials, EMPA, Dübendorf, Switzerland.</nlm:affiliation></affiliation></author><author><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name><affiliation><nlm:affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>VIB Center for Plant Systems Biology, Ghent, Belgium.</nlm:affiliation></affiliation></author><author><name sortKey="Ruggeberg, Markus" sort="Ruggeberg, Markus" uniqKey="Ruggeberg M" first="Markus" last="Rüggeberg">Markus Rüggeberg</name><affiliation><nlm:affiliation>Institute for Building Materials (IfB), ETH Zurich, Zurich, Switzerland.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Cellulose and Wood Materials, EMPA, Dübendorf, Switzerland.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Elastic Modulus (physiology)</term><term>Lignin (metabolism)</term><term>Microfibrils (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Microfibrils</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Elastic Modulus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Wood is extensively used as a construction material. Despite increasing knowledge of its mechanical properties, the contribution of the cell-wall matrix polymers to wood mechanics is still not well understood. Previous studies have shown that axial stiffness correlates with lignin content only for cellulose microfibril angles larger than around 20°, while no influence is found for smaller angles. Here, by analysing the wood of poplar with reduced lignin content due to down-regulation of CAFFEOYL SHIKIMATE ESTERASE, we show that lignin content also influences axial stiffness at smaller angles. Micro-tensile tests of the xylem revealed that axial stiffness was strongly reduced in the low-lignin transgenic lines. Strikingly, microfibril angles were around 15° for both wild-type and transgenic poplars, suggesting that cellulose orientation is not responsible for the observed changes in mechanical behavior. Multiple linear regression analysis showed that the decrease in stiffness was almost completely related to the variation in both density and lignin content. We suggest that the influence of lignin content on axial stiffness may gradually increase as a function of the microfibril angle. Our results may help in building up comprehensive models of the cell wall that can unravel the individual roles of the matrix polymers.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31187131</PMID><DateCompleted><Year>2020</Year><Month>07</Month><Day>20</Day></DateCompleted><DateRevised><Year>2020</Year><Month>07</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><Volume>70</Volume><Issue>15</Issue><PubDate><Year>2019</Year><Month>08</Month><Day>07</Day></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>Significant influence of lignin on axial elastic modulus of poplar wood at low microfibril angles under wet conditions.</ArticleTitle><Pagination><MedlinePgn>4039-4047</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/erz180</ELocationID><Abstract><AbstractText>Wood is extensively used as a construction material. Despite increasing knowledge of its mechanical properties, the contribution of the cell-wall matrix polymers to wood mechanics is still not well understood. Previous studies have shown that axial stiffness correlates with lignin content only for cellulose microfibril angles larger than around 20°, while no influence is found for smaller angles. Here, by analysing the wood of poplar with reduced lignin content due to down-regulation of CAFFEOYL SHIKIMATE ESTERASE, we show that lignin content also influences axial stiffness at smaller angles. Micro-tensile tests of the xylem revealed that axial stiffness was strongly reduced in the low-lignin transgenic lines. Strikingly, microfibril angles were around 15° for both wild-type and transgenic poplars, suggesting that cellulose orientation is not responsible for the observed changes in mechanical behavior. Multiple linear regression analysis showed that the decrease in stiffness was almost completely related to the variation in both density and lignin content. We suggest that the influence of lignin content on axial stiffness may gradually increase as a function of the microfibril angle. Our results may help in building up comprehensive models of the cell wall that can unravel the individual roles of the matrix polymers.</AbstractText><CopyrightInformation>© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Özparpucu</LastName><ForeName>Merve</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute for Building Materials (IfB), ETH Zurich, Zurich, Switzerland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Life Sciences Weihenstephan, Wood Research Munich, Technical University of Munich (TUM), Munich, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gierlinger</LastName><ForeName>Notburga</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Institute for Biophysics, University of Natural Resources and Life Sciences Vienna (BOKU), Wien, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cesarino</LastName><ForeName>Igor</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo - SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Burgert</LastName><ForeName>Ingo</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Institute for Building Materials (IfB), ETH Zurich, Zurich, Switzerland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Cellulose and Wood Materials, EMPA, Dübendorf, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boerjan</LastName><ForeName>Wout</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rüggeberg</LastName><ForeName>Markus</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute for Building Materials (IfB), ETH Zurich, Zurich, Switzerland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Cellulose and Wood Materials, EMPA, Dübendorf, Switzerland.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D055119" MajorTopicYN="N">Elastic Modulus</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020894" MajorTopicYN="N">Microfibrils</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Populus tremula×Populus alba</Keyword><Keyword MajorTopicYN="Y">CAFFEOYL SHIKIMATE ESTERASE (CSE)</Keyword><Keyword MajorTopicYN="Y">cell-wall mechanics</Keyword><Keyword MajorTopicYN="Y">lignin</Keyword><Keyword MajorTopicYN="Y">lignin engineering</Keyword><Keyword MajorTopicYN="Y">micromechanics</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>11</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>04</Month><Day>02</Day></PubMedPubDate><PubMedPubDate 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