Bacillus thuringiensis cry1C expression from the plastid genome of poplar leads to high mortality of leaf-eating caterpillars.
Identifieur interne : 000831 ( Main/Corpus ); précédent : 000830; suivant : 000832Bacillus thuringiensis cry1C expression from the plastid genome of poplar leads to high mortality of leaf-eating caterpillars.
Auteurs : Yuyong Wu ; Letian Xu ; Ling Chang ; Meiqi Ma ; Lili You ; Chunmei Jiang ; Shengchun Li ; Jiang ZhangSource :
- Tree physiology [ 1758-4469 ] ; 2019.
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Abstract
Plastid transformation technology has several attractive features compared with traditional nuclear transformation technology. However, only a handful of species are able to be successfully transformed. Here, we report an efficient and stable plastid transformation protocol for poplar, an economically important tree species grown worldwide. We transformed the Bacillus thuringiensis cry1C gene into the poplar plastid genome, and homoplasmic transplastomic poplar was obtained after two to three rounds of regeneration under antibiotic selection for 7-12 months. The transplastomic poplar expressing Cry1C insecticidal protein showed the highest accumulation level in young leaves, which reached up to 20.7 μg g-1 fresh weight, and comparatively low levels in mature and old leaves, and hardly detectable levels in non-green tissues, such as phloem, xylem and roots. Transplastomic poplar showed high toxicity to Hyphantria cunea and Lymantria dispar, two notorious forest pests worldwide, without affecting plant growth. These results are the first successful examples of insect-resistant poplar generation by plastid genome engineering and provide a new avenue for future genetic improvement of poplar plants.
DOI: 10.1093/treephys/tpz073
PubMed: 31222266
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School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ma, Meiqi" sort="Ma, Meiqi" uniqKey="Ma M" first="Meiqi" last="Ma">Meiqi Ma</name><affiliation><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author><author><name sortKey="You, Lili" sort="You, Lili" uniqKey="You L" first="Lili" last="You">Lili You</name><affiliation><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author><author><name sortKey="Jiang, Chunmei" sort="Jiang, Chunmei" uniqKey="Jiang C" first="Chunmei" last="Jiang">Chunmei Jiang</name><affiliation><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Shengchun" sort="Li, Shengchun" uniqKey="Li S" first="Shengchun" last="Li">Shengchun Li</name><affiliation><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Jiang" sort="Zhang, Jiang" uniqKey="Zhang J" first="Jiang" last="Zhang">Jiang Zhang</name><affiliation><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31222266</idno><idno type="pmid">31222266</idno><idno type="doi">10.1093/treephys/tpz073</idno><idno type="wicri:Area/Main/Corpus">000831</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000831</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Bacillus thuringiensis cry1C expression from the plastid genome of poplar leads to high mortality of leaf-eating caterpillars.</title><author><name sortKey="Wu, Yuyong" sort="Wu, Yuyong" uniqKey="Wu Y" first="Yuyong" last="Wu">Yuyong Wu</name><affiliation><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Letian" sort="Xu, Letian" uniqKey="Xu L" first="Letian" last="Xu">Letian Xu</name><affiliation><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chang, Ling" sort="Chang, Ling" uniqKey="Chang L" first="Ling" last="Chang">Ling Chang</name><affiliation><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ma, Meiqi" sort="Ma, Meiqi" uniqKey="Ma M" first="Meiqi" last="Ma">Meiqi Ma</name><affiliation><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author><author><name sortKey="You, Lili" sort="You, Lili" uniqKey="You L" first="Lili" last="You">Lili You</name><affiliation><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author><author><name sortKey="Jiang, Chunmei" sort="Jiang, Chunmei" uniqKey="Jiang C" first="Chunmei" last="Jiang">Chunmei Jiang</name><affiliation><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Shengchun" sort="Li, Shengchun" uniqKey="Li S" first="Shengchun" last="Li">Shengchun Li</name><affiliation><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Jiang" sort="Zhang, Jiang" uniqKey="Zhang J" first="Jiang" last="Zhang">Jiang Zhang</name><affiliation><nlm:affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Bacillus thuringiensis (MeSH)</term><term>Genome, Plastid (MeSH)</term><term>Larva (MeSH)</term><term>Plant Leaves (MeSH)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (MeSH)</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Bacillus thuringiensis</term><term>Genome, Plastid</term><term>Larva</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plastid transformation technology has several attractive features compared with traditional nuclear transformation technology. However, only a handful of species are able to be successfully transformed. Here, we report an efficient and stable plastid transformation protocol for poplar, an economically important tree species grown worldwide. We transformed the Bacillus thuringiensis cry1C gene into the poplar plastid genome, and homoplasmic transplastomic poplar was obtained after two to three rounds of regeneration under antibiotic selection for 7-12 months. The transplastomic poplar expressing Cry1C insecticidal protein showed the highest accumulation level in young leaves, which reached up to 20.7 μg g-1 fresh weight, and comparatively low levels in mature and old leaves, and hardly detectable levels in non-green tissues, such as phloem, xylem and roots. Transplastomic poplar showed high toxicity to Hyphantria cunea and Lymantria dispar, two notorious forest pests worldwide, without affecting plant growth. These results are the first successful examples of insect-resistant poplar generation by plastid genome engineering and provide a new avenue for future genetic improvement of poplar plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">31222266</PMID><DateCompleted><Year>2020</Year><Month>05</Month><Day>18</Day></DateCompleted><DateRevised><Year>2020</Year><Month>05</Month><Day>18</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><Volume>39</Volume><Issue>9</Issue><PubDate><Year>2019</Year><Month>09</Month><Day>01</Day></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Bacillus thuringiensis cry1C expression from the plastid genome of poplar leads to high mortality of leaf-eating caterpillars.</ArticleTitle><Pagination><MedlinePgn>1525-1532</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpz073</ELocationID><Abstract><AbstractText>Plastid transformation technology has several attractive features compared with traditional nuclear transformation technology. However, only a handful of species are able to be successfully transformed. Here, we report an efficient and stable plastid transformation protocol for poplar, an economically important tree species grown worldwide. We transformed the Bacillus thuringiensis cry1C gene into the poplar plastid genome, and homoplasmic transplastomic poplar was obtained after two to three rounds of regeneration under antibiotic selection for 7-12 months. The transplastomic poplar expressing Cry1C insecticidal protein showed the highest accumulation level in young leaves, which reached up to 20.7 μg g-1 fresh weight, and comparatively low levels in mature and old leaves, and hardly detectable levels in non-green tissues, such as phloem, xylem and roots. Transplastomic poplar showed high toxicity to Hyphantria cunea and Lymantria dispar, two notorious forest pests worldwide, without affecting plant growth. These results are the first successful examples of insect-resistant poplar generation by plastid genome engineering and provide a new avenue for future genetic improvement of poplar plants.</AbstractText><CopyrightInformation>© The Author(s) 2019. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Yuyong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Letian</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Ling</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Meiqi</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>You</LastName><ForeName>Lili</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Chunmei</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Shengchun</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jiang</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016422">Letter</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001413" MajorTopicYN="Y">Bacillus thuringiensis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054627" MajorTopicYN="Y">Genome, Plastid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007814" MajorTopicYN="N">Larva</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="Y">Populus</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Bacillus thuringiensis
</Keyword><Keyword MajorTopicYN="Y">caterpillars</Keyword><Keyword MajorTopicYN="Y">insect resistance</Keyword><Keyword MajorTopicYN="Y">plastid genome engineering</Keyword><Keyword MajorTopicYN="Y">poplar</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>01</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>04</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>06</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>6</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>5</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>6</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31222266</ArticleId><ArticleId IdType="pii">5521193</ArticleId><ArticleId IdType="doi">10.1093/treephys/tpz073</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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