Integrated physiological and genomic analysis reveals structural variations and expression patterns of candidate genes for colored- and green-leaf poplar.
Identifieur interne : 000772 ( Main/Corpus ); précédent : 000771; suivant : 000773Integrated physiological and genomic analysis reveals structural variations and expression patterns of candidate genes for colored- and green-leaf poplar.
Auteurs : Weibing Zhuang ; Hongxue Wang ; Tianyu Liu ; Tao Wang ; Fengjiao Zhang ; Xiaochun Shu ; Henghua Zhai ; Zhong WangSource :
- Scientific reports [ 2045-2322 ] ; 2019.
English descriptors
- KwdEn :
- Anthocyanins (analysis), Anthocyanins (biosynthesis), Anthocyanins (genetics), Carotenoids (analysis), Color (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (genetics), Genetic Variation (MeSH), Genome, Plant (genetics), Genomics (MeSH), Pigmentation (genetics), Plant Leaves (chemistry), Plant Leaves (genetics), Populus (genetics).
- MESH :
- chemical , analysis : Anthocyanins, Carotenoids.
- chemical , biosynthesis : Anthocyanins.
- chemical , genetics : Anthocyanins.
- chemistry : Plant Leaves.
- genetics : Genes, Plant, Genome, Plant, Pigmentation, Plant Leaves, Populus.
- Color, Gene Expression Regulation, Plant, Genetic Variation, Genomics.
Abstract
Colored-leaf plants are increasingly popular and have been attracting more and more attentions. However, the molecular mechanism of leaf coloration in plants has not been fully understood. In this study, a colored-leaf cultivar of Populus deltoides (Caihong poplar, CHP) and green-leaf cultivar of Populus deltoides L2025 were used to explore the mechanism of leaf coloration through physiological and the whole genome resequencing analysis. The content of anthocyanins, total Chl, and carotenoids in the leaves of CHP and L2025 were evaluated. The ratio of anthocyanins to total Chl in CHP was 25.0 times higher than that in L2025; this could be attributed to the red leaf color of CHP. Based on the whole genome resequencing analysis, 951,421 polymorphic SNPs and 221,907 indels were screened between CHP and L2025. Using qRT-PCR analysis, three structural genes (flavonol synthase 1 family protein, UDP-glucose flavonoid 3-O-glucosyltransferase 3' and flavonoid 3-O-galactosyl transferase family protein) and six transcription factors (MYB-related protein Myb4, transcription factor GAMYB, PtrMYB179, transcription factor bHLH53, transcription factor bHLH3, VARICOSE family protein) may be involved in the anthocyanin synthesis pathway, which could be used as candidate genes to explore the molecular regulation mechanism of leaf coloration in Populus deltoids, and could be used in molecular breeding in the future.
DOI: 10.1038/s41598-019-47681-9
PubMed: 31371772
PubMed Central: PMC6673700
Links to Exploration step
pubmed:31371772Le document en format XML
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Sun Yat-Sen), Nanjing, 210014, China. weibingzhuangnj@sina.com.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Hongxue" sort="Wang, Hongxue" uniqKey="Wang H" first="Hongxue" last="Wang">Hongxue Wang</name><affiliation><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Tianyu" sort="Liu, Tianyu" uniqKey="Liu T" first="Tianyu" last="Liu">Tianyu Liu</name><affiliation><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Tao" sort="Wang, Tao" uniqKey="Wang T" first="Tao" last="Wang">Tao Wang</name><affiliation><nlm:affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. 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Sun Yat-Sen), Nanjing, 210014, China. weibingzhuangnj@sina.com.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Hongxue" sort="Wang, Hongxue" uniqKey="Wang H" first="Hongxue" last="Wang">Hongxue Wang</name><affiliation><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Tianyu" sort="Liu, Tianyu" uniqKey="Liu T" first="Tianyu" last="Liu">Tianyu Liu</name><affiliation><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Tao" sort="Wang, Tao" uniqKey="Wang T" first="Tao" last="Wang">Tao Wang</name><affiliation><nlm:affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Fengjiao" sort="Zhang, Fengjiao" uniqKey="Zhang F" first="Fengjiao" last="Zhang">Fengjiao Zhang</name><affiliation><nlm:affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China.</nlm:affiliation></affiliation></author><author><name sortKey="Shu, Xiaochun" sort="Shu, Xiaochun" uniqKey="Shu X" first="Xiaochun" last="Shu">Xiaochun Shu</name><affiliation><nlm:affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhai, Henghua" sort="Zhai, Henghua" uniqKey="Zhai H" first="Henghua" last="Zhai">Henghua Zhai</name><affiliation><nlm:affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Zhong" sort="Wang, Zhong" uniqKey="Wang Z" first="Zhong" last="Wang">Zhong Wang</name><affiliation><nlm:affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China. wangzhong19@163.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anthocyanins (analysis)</term><term>Anthocyanins (biosynthesis)</term><term>Anthocyanins (genetics)</term><term>Carotenoids (analysis)</term><term>Color (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (genetics)</term><term>Genetic Variation (MeSH)</term><term>Genome, Plant (genetics)</term><term>Genomics (MeSH)</term><term>Pigmentation (genetics)</term><term>Plant Leaves (chemistry)</term><term>Plant Leaves (genetics)</term><term>Populus (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Anthocyanins</term><term>Carotenoids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Anthocyanins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Anthocyanins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, Plant</term><term>Genome, Plant</term><term>Pigmentation</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Color</term><term>Gene Expression Regulation, Plant</term><term>Genetic Variation</term><term>Genomics</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Colored-leaf plants are increasingly popular and have been attracting more and more attentions. However, the molecular mechanism of leaf coloration in plants has not been fully understood. In this study, a colored-leaf cultivar of Populus deltoides (Caihong poplar, CHP) and green-leaf cultivar of Populus deltoides L2025 were used to explore the mechanism of leaf coloration through physiological and the whole genome resequencing analysis. The content of anthocyanins, total Chl, and carotenoids in the leaves of CHP and L2025 were evaluated. The ratio of anthocyanins to total Chl in CHP was 25.0 times higher than that in L2025; this could be attributed to the red leaf color of CHP. Based on the whole genome resequencing analysis, 951,421 polymorphic SNPs and 221,907 indels were screened between CHP and L2025. Using qRT-PCR analysis, three structural genes (flavonol synthase 1 family protein, UDP-glucose flavonoid 3-O-glucosyltransferase 3' and flavonoid 3-O-galactosyl transferase family protein) and six transcription factors (MYB-related protein Myb4, transcription factor GAMYB, PtrMYB179, transcription factor bHLH53, transcription factor bHLH3, VARICOSE family protein) may be involved in the anthocyanin synthesis pathway, which could be used as candidate genes to explore the molecular regulation mechanism of leaf coloration in Populus deltoids, and could be used in molecular breeding in the future.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31371772</PMID><DateCompleted><Year>2020</Year><Month>11</Month><Day>05</Day></DateCompleted><DateRevised><Year>2020</Year><Month>11</Month><Day>05</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>08</Month><Day>01</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Integrated physiological and genomic analysis reveals structural variations and expression patterns of candidate genes for colored- and green-leaf poplar.</ArticleTitle><Pagination><MedlinePgn>11150</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-019-47681-9</ELocationID><Abstract><AbstractText>Colored-leaf plants are increasingly popular and have been attracting more and more attentions. However, the molecular mechanism of leaf coloration in plants has not been fully understood. In this study, a colored-leaf cultivar of Populus deltoides (Caihong poplar, CHP) and green-leaf cultivar of Populus deltoides L2025 were used to explore the mechanism of leaf coloration through physiological and the whole genome resequencing analysis. The content of anthocyanins, total Chl, and carotenoids in the leaves of CHP and L2025 were evaluated. The ratio of anthocyanins to total Chl in CHP was 25.0 times higher than that in L2025; this could be attributed to the red leaf color of CHP. Based on the whole genome resequencing analysis, 951,421 polymorphic SNPs and 221,907 indels were screened between CHP and L2025. Using qRT-PCR analysis, three structural genes (flavonol synthase 1 family protein, UDP-glucose flavonoid 3-O-glucosyltransferase 3' and flavonoid 3-O-galactosyl transferase family protein) and six transcription factors (MYB-related protein Myb4, transcription factor GAMYB, PtrMYB179, transcription factor bHLH53, transcription factor bHLH3, VARICOSE family protein) may be involved in the anthocyanin synthesis pathway, which could be used as candidate genes to explore the molecular regulation mechanism of leaf coloration in Populus deltoids, and could be used in molecular breeding in the future.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhuang</LastName><ForeName>Weibing</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China. weibingzhuangnj@sina.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Hongxue</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Tianyu</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Tao</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Fengjiao</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shu</LastName><ForeName>Xiaochun</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhai</LastName><ForeName>Henghua</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Zhong</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China. wangzhong19@163.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>08</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000872">Anthocyanins</NameOfSubstance></Chemical><Chemical><RegistryNumber>36-88-4</RegistryNumber><NameOfSubstance UI="D002338">Carotenoids</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000872" MajorTopicYN="N">Anthocyanins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002338" MajorTopicYN="N">Carotenoids</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003116" MajorTopicYN="N">Color</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="N">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018745" MajorTopicYN="N">Genome, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D023281" MajorTopicYN="Y">Genomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010858" MajorTopicYN="N">Pigmentation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>03</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>07</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>8</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>8</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31371772</ArticleId><ArticleId IdType="doi">10.1038/s41598-019-47681-9</ArticleId><ArticleId IdType="pii">10.1038/s41598-019-47681-9</ArticleId><ArticleId IdType="pmc">PMC6673700</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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