Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus.
Identifieur interne : 000721 ( Main/Corpus ); précédent : 000720; suivant : 000722Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus.
Auteurs : Jin Zhang ; Meng Xie ; Mi Li ; Jinhua Ding ; Yunqiao Pu ; Anthony C. Bryan ; William Rottmann ; Kimberly A. Winkeler ; Cassandra M. Collins ; Vasanth Singan ; Erika A. Lindquist ; Sara S. Jawdy ; Lee E. Gunter ; Nancy L. Engle ; Xiaohan Yang ; Kerrie Barry ; Timothy J. Tschaplinski ; Jeremy Schmutz ; Gerald A. Tuskan ; Wellington Muchero ; Jin-Gui ChenSource :
- Plant biotechnology journal [ 1467-7652 ] ; 2020.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Molecular Chaperones.
- chemical : Lignin.
- genetics : Populus.
- Biomass, Genes, Plant, Plants, Genetically Modified.
Abstract
Prefoldin (PFD) is a group II chaperonin that is ubiquitously present in the eukaryotic kingdom. Six subunits (PFD1-6) form a jellyfish-like heterohexameric PFD complex and function in protein folding and cytoskeleton organization. However, little is known about its function in plant cell wall-related processes. Here, we report the functional characterization of a PFD gene from Populus deltoides, designated as PdPFD2.2. There are two copies of PFD2 in Populus, and PdPFD2.2 was ubiquitously expressed with high transcript abundance in the cambial region. PdPFD2.2 can physically interact with DELLA protein RGA1_8g, and its subcellular localization is affected by the interaction. In P. deltoides transgenic plants overexpressing PdPFD2.2, the lignin syringyl/guaiacyl ratio was increased, but cellulose content and crystallinity index were unchanged. In addition, the total released sugar (glucose and xylose) amounts were increased by 7.6% and 6.1%, respectively, in two transgenic lines. Transcriptomic and metabolomic analyses revealed that secondary metabolic pathways, including lignin and flavonoid biosynthesis, were affected by overexpressing PdPFD2.2. A total of eight hub transcription factors (TFs) were identified based on TF binding sites of differentially expressed genes in Populus transgenic plants overexpressing PdPFD2.2. In addition, several known cell wall-related TFs, such as MYB3, MYB4, MYB7, TT8 and XND1, were affected by overexpression of PdPFD2.2. These results suggest that overexpression of PdPFD2.2 can reduce biomass recalcitrance and PdPFD2.2 is a promising target for genetic engineering to improve feedstock characteristics to enhance biofuel conversion and reduce the cost of lignocellulosic biofuel production.
DOI: 10.1111/pbi.13254
PubMed: 31498543
PubMed Central: PMC7004918
Links to Exploration step
pubmed:31498543Le document en format XML
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Collins</name><affiliation><nlm:affiliation>ArborGen Inc., Ridgeville, SC, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Singan, Vasanth" sort="Singan, Vasanth" uniqKey="Singan V" first="Vasanth" last="Singan">Vasanth Singan</name><affiliation><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Lindquist, Erika A" sort="Lindquist, Erika A" uniqKey="Lindquist E" first="Erika A" last="Lindquist">Erika A. Lindquist</name><affiliation><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Jawdy, Sara S" sort="Jawdy, Sara S" uniqKey="Jawdy S" first="Sara S" last="Jawdy">Sara S. Jawdy</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Gunter, Lee E" sort="Gunter, Lee E" uniqKey="Gunter L" first="Lee E" last="Gunter">Lee E. Gunter</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Engle, Nancy L" sort="Engle, Nancy L" uniqKey="Engle N" first="Nancy L" last="Engle">Nancy L. Engle</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Xiaohan" sort="Yang, Xiaohan" uniqKey="Yang X" first="Xiaohan" last="Yang">Xiaohan Yang</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Barry, Kerrie" sort="Barry, Kerrie" uniqKey="Barry K" first="Kerrie" last="Barry">Kerrie Barry</name><affiliation><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Schmutz, Jeremy" sort="Schmutz, Jeremy" uniqKey="Schmutz J" first="Jeremy" last="Schmutz">Jeremy Schmutz</name><affiliation><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Muchero, Wellington" sort="Muchero, Wellington" uniqKey="Muchero W" first="Wellington" last="Muchero">Wellington Muchero</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Jin Gui" sort="Chen, Jin Gui" uniqKey="Chen J" first="Jin-Gui" last="Chen">Jin-Gui Chen</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31498543</idno><idno type="pmid">31498543</idno><idno type="doi">10.1111/pbi.13254</idno><idno type="pmc">PMC7004918</idno><idno type="wicri:Area/Main/Corpus">000721</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000721</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus.</title><author><name sortKey="Zhang, Jin" sort="Zhang, Jin" uniqKey="Zhang J" first="Jin" last="Zhang">Jin Zhang</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Xie, Meng" sort="Xie, Meng" uniqKey="Xie M" first="Meng" last="Xie">Meng Xie</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Plant Sciences, University of Tennessee, Knoxville, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Mi" sort="Li, Mi" uniqKey="Li M" first="Mi" last="Li">Mi Li</name><affiliation><nlm:affiliation>Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ding, Jinhua" sort="Ding, Jinhua" uniqKey="Ding J" first="Jinhua" last="Ding">Jinhua Ding</name><affiliation><nlm:affiliation>Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Textiles, Donghua University, Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Pu, Yunqiao" sort="Pu, Yunqiao" uniqKey="Pu Y" first="Yunqiao" last="Pu">Yunqiao Pu</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Bryan, Anthony C" sort="Bryan, Anthony C" uniqKey="Bryan A" first="Anthony C" last="Bryan">Anthony C. Bryan</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Rottmann, William" sort="Rottmann, William" uniqKey="Rottmann W" first="William" last="Rottmann">William Rottmann</name><affiliation><nlm:affiliation>ArborGen Inc., Ridgeville, SC, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Winkeler, Kimberly A" sort="Winkeler, Kimberly A" uniqKey="Winkeler K" first="Kimberly A" last="Winkeler">Kimberly A. Winkeler</name><affiliation><nlm:affiliation>ArborGen Inc., Ridgeville, SC, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Collins, Cassandra M" sort="Collins, Cassandra M" uniqKey="Collins C" first="Cassandra M" last="Collins">Cassandra M. Collins</name><affiliation><nlm:affiliation>ArborGen Inc., Ridgeville, SC, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Singan, Vasanth" sort="Singan, Vasanth" uniqKey="Singan V" first="Vasanth" last="Singan">Vasanth Singan</name><affiliation><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Lindquist, Erika A" sort="Lindquist, Erika A" uniqKey="Lindquist E" first="Erika A" last="Lindquist">Erika A. Lindquist</name><affiliation><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Jawdy, Sara S" sort="Jawdy, Sara S" uniqKey="Jawdy S" first="Sara S" last="Jawdy">Sara S. Jawdy</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Gunter, Lee E" sort="Gunter, Lee E" uniqKey="Gunter L" first="Lee E" last="Gunter">Lee E. Gunter</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Engle, Nancy L" sort="Engle, Nancy L" uniqKey="Engle N" first="Nancy L" last="Engle">Nancy L. Engle</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Xiaohan" sort="Yang, Xiaohan" uniqKey="Yang X" first="Xiaohan" last="Yang">Xiaohan Yang</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Barry, Kerrie" sort="Barry, Kerrie" uniqKey="Barry K" first="Kerrie" last="Barry">Kerrie Barry</name><affiliation><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Schmutz, Jeremy" sort="Schmutz, Jeremy" uniqKey="Schmutz J" first="Jeremy" last="Schmutz">Jeremy Schmutz</name><affiliation><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Muchero, Wellington" sort="Muchero, Wellington" uniqKey="Muchero W" first="Wellington" last="Muchero">Wellington Muchero</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Jin Gui" sort="Chen, Jin Gui" uniqKey="Chen J" first="Jin-Gui" last="Chen">Jin-Gui Chen</name><affiliation><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Plant biotechnology journal</title><idno type="eISSN">1467-7652</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biomass (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Lignin (MeSH)</term><term>Molecular Chaperones (genetics)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Molecular Chaperones</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>Genes, Plant</term><term>Plants, Genetically Modified</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Prefoldin (PFD) is a group II chaperonin that is ubiquitously present in the eukaryotic kingdom. Six subunits (PFD1-6) form a jellyfish-like heterohexameric PFD complex and function in protein folding and cytoskeleton organization. However, little is known about its function in plant cell wall-related processes. Here, we report the functional characterization of a PFD gene from Populus deltoides, designated as PdPFD2.2. There are two copies of PFD2 in Populus, and PdPFD2.2 was ubiquitously expressed with high transcript abundance in the cambial region. PdPFD2.2 can physically interact with DELLA protein RGA1_8g, and its subcellular localization is affected by the interaction. In P. deltoides transgenic plants overexpressing PdPFD2.2, the lignin syringyl/guaiacyl ratio was increased, but cellulose content and crystallinity index were unchanged. In addition, the total released sugar (glucose and xylose) amounts were increased by 7.6% and 6.1%, respectively, in two transgenic lines. Transcriptomic and metabolomic analyses revealed that secondary metabolic pathways, including lignin and flavonoid biosynthesis, were affected by overexpressing PdPFD2.2. A total of eight hub transcription factors (TFs) were identified based on TF binding sites of differentially expressed genes in Populus transgenic plants overexpressing PdPFD2.2. In addition, several known cell wall-related TFs, such as MYB3, MYB4, MYB7, TT8 and XND1, were affected by overexpression of PdPFD2.2. These results suggest that overexpression of PdPFD2.2 can reduce biomass recalcitrance and PdPFD2.2 is a promising target for genetic engineering to improve feedstock characteristics to enhance biofuel conversion and reduce the cost of lignocellulosic biofuel production.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">31498543</PMID><DateCompleted><Year>2020</Year><Month>08</Month><Day>06</Day></DateCompleted><DateRevised><Year>2020</Year><Month>08</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1467-7652</ISSN><JournalIssue CitedMedium="Internet"><Volume>18</Volume><Issue>3</Issue><PubDate><Year>2020</Year><Month>03</Month></PubDate></JournalIssue><Title>Plant biotechnology journal</Title><ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus.</ArticleTitle><Pagination><MedlinePgn>859-871</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/pbi.13254</ELocationID><Abstract><AbstractText>Prefoldin (PFD) is a group II chaperonin that is ubiquitously present in the eukaryotic kingdom. Six subunits (PFD1-6) form a jellyfish-like heterohexameric PFD complex and function in protein folding and cytoskeleton organization. However, little is known about its function in plant cell wall-related processes. Here, we report the functional characterization of a PFD gene from Populus deltoides, designated as PdPFD2.2. There are two copies of PFD2 in Populus, and PdPFD2.2 was ubiquitously expressed with high transcript abundance in the cambial region. PdPFD2.2 can physically interact with DELLA protein RGA1_8g, and its subcellular localization is affected by the interaction. In P. deltoides transgenic plants overexpressing PdPFD2.2, the lignin syringyl/guaiacyl ratio was increased, but cellulose content and crystallinity index were unchanged. In addition, the total released sugar (glucose and xylose) amounts were increased by 7.6% and 6.1%, respectively, in two transgenic lines. Transcriptomic and metabolomic analyses revealed that secondary metabolic pathways, including lignin and flavonoid biosynthesis, were affected by overexpressing PdPFD2.2. A total of eight hub transcription factors (TFs) were identified based on TF binding sites of differentially expressed genes in Populus transgenic plants overexpressing PdPFD2.2. In addition, several known cell wall-related TFs, such as MYB3, MYB4, MYB7, TT8 and XND1, were affected by overexpression of PdPFD2.2. These results suggest that overexpression of PdPFD2.2 can reduce biomass recalcitrance and PdPFD2.2 is a promising target for genetic engineering to improve feedstock characteristics to enhance biofuel conversion and reduce the cost of lignocellulosic biofuel production.</AbstractText><CopyrightInformation>© 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jin</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0002-8397-5078</Identifier><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Meng</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Plant Sciences, University of Tennessee, Knoxville, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Mi</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Jinhua</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Textiles, Donghua University, Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pu</LastName><ForeName>Yunqiao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bryan</LastName><ForeName>Anthony C</ForeName><Initials>AC</Initials><Identifier Source="ORCID">0000-0003-0917-8705</Identifier><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rottmann</LastName><ForeName>William</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>ArborGen Inc., Ridgeville, SC, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Winkeler</LastName><ForeName>Kimberly A</ForeName><Initials>KA</Initials><AffiliationInfo><Affiliation>ArborGen Inc., Ridgeville, SC, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Collins</LastName><ForeName>Cassandra M</ForeName><Initials>CM</Initials><AffiliationInfo><Affiliation>ArborGen Inc., Ridgeville, SC, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Singan</LastName><ForeName>Vasanth</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lindquist</LastName><ForeName>Erika A</ForeName><Initials>EA</Initials><AffiliationInfo><Affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jawdy</LastName><ForeName>Sara S</ForeName><Initials>SS</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gunter</LastName><ForeName>Lee E</ForeName><Initials>LE</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Engle</LastName><ForeName>Nancy L</ForeName><Initials>NL</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xiaohan</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barry</LastName><ForeName>Kerrie</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tschaplinski</LastName><ForeName>Timothy J</ForeName><Initials>TJ</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schmutz</LastName><ForeName>Jeremy</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tuskan</LastName><ForeName>Gerald A</ForeName><Initials>GA</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Muchero</LastName><ForeName>Wellington</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Jin-Gui</ForeName><Initials>JG</Initials><Identifier Source="ORCID">0000-0002-1752-4201</Identifier><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>09</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biotechnol J</MedlineTA><NlmUniqueID>101201889</NlmUniqueID><ISSNLinking>1467-7644</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018832">Molecular Chaperones</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C112806">prefoldin</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="Y">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018832" MajorTopicYN="N">Molecular Chaperones</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Populus
</Keyword><Keyword MajorTopicYN="Y">S/G ratio</Keyword><Keyword MajorTopicYN="Y">biofuels</Keyword><Keyword MajorTopicYN="Y">cell wall recalcitrance</Keyword><Keyword MajorTopicYN="Y">lignin</Keyword><Keyword MajorTopicYN="Y">metabolome</Keyword><Keyword MajorTopicYN="Y">prefoldin</Keyword><Keyword MajorTopicYN="Y">transcriptome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>03</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>08</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>09</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>9</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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