Over-expression of poplar NAC15 gene enhances wood formation in transgenic tobacco.
Identifieur interne : 000522 ( Main/Corpus ); précédent : 000521; suivant : 000523Over-expression of poplar NAC15 gene enhances wood formation in transgenic tobacco.
Auteurs : Wenjing Yao ; Dawei Zhang ; Boru Zhou ; Jianping Wang ; Renhua Li ; Tingbo JiangSource :
- BMC plant biology [ 1471-2229 ] ; 2020.
English descriptors
- KwdEn :
- Cell Wall (metabolism), Cellulose (metabolism), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Lignin (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (genetics), Tobacco (genetics), Tobacco (metabolism), Transcription Factors (genetics), Transcription Factors (metabolism), Wood (metabolism), Xylem (metabolism).
- MESH :
- chemical , genetics : Plant Proteins, Transcription Factors.
- chemical , metabolism : Cellulose, Lignin, Plant Proteins, Transcription Factors.
- genetics : Plants, Genetically Modified, Populus, Tobacco.
- metabolism : Cell Wall, Plants, Genetically Modified, Tobacco, Wood, Xylem.
- Gene Expression Profiling, Gene Expression Regulation, Plant.
Abstract
BACKGROUND
NAC (NAM/ATAF/CUC) is one of the largest plant-specific transcription factor (TF) families known to play significant roles in wood formation. Acting as master gene regulators, a few NAC genes can activate secondary wall biosynthesis during wood formation in woody plants.
RESULTS
In the present study, firstly, we screened 110 differentially expressed NAC genes in the leaves, stems, and roots of di-haploid Populus simonii×P. nigra by RNA-Seq. Then we identified a nucleus-targeted gene, NAC15 gene, which was one of the highly expressed genes in the stem among 110 NAC family members. Thirdly, we conducted expression pattern analysis of NAC15 gene, and observed NAC15 gene was most highly expressed in the xylem by RT-qPCR. Moreover, we transferred NAC15 gene into tobacco and obtained 12 transgenic lines overexpressing NAC15 gene (TLs). And the relative higher content of hemicellulose, cellulose and lignin was observed in the TLs compared to the control lines containing empty vector (CLs). It also showed darker staining in the culms of the TLs with phloroglucinol staining, compared to the CLs. Furthermore, the relative expression level of a few lignin- and cellulose-related genes was significantly higher in the TLs than that in the CLs.
CONCLUSIONS
The overall results indicated that NAC15 gene is highly expressed in the xylem of poplar and may be a potential candidate gene playing an important role in wood formation in transgenic tobacco.
DOI: 10.1186/s12870-019-2191-2
PubMed: 31914923
PubMed Central: PMC6950812
Links to Exploration step
pubmed:31914923Le document en format XML
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China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Boru" sort="Zhou, Boru" uniqKey="Zhou B" first="Boru" last="Zhou">Boru Zhou</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China. 896099005@qq.com.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Jianping" sort="Wang, Jianping" uniqKey="Wang J" first="Jianping" last="Wang">Jianping Wang</name><affiliation><nlm:affiliation>Department of Agronomy, University of Florida, 2033 Mowry Road, Gainesville, FL, 32610, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Renhua" sort="Li, Renhua" uniqKey="Li R" first="Renhua" last="Li">Renhua Li</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.</nlm:affiliation></affiliation></author><author><name sortKey="Jiang, Tingbo" sort="Jiang, Tingbo" uniqKey="Jiang T" first="Tingbo" last="Jiang">Tingbo Jiang</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China. tbjiang@yahoo.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31914923</idno><idno type="pmid">31914923</idno><idno type="doi">10.1186/s12870-019-2191-2</idno><idno type="pmc">PMC6950812</idno><idno type="wicri:Area/Main/Corpus">000522</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000522</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Over-expression of poplar NAC15 gene enhances wood formation in transgenic tobacco.</title><author><name sortKey="Yao, Wenjing" sort="Yao, Wenjing" uniqKey="Yao W" first="Wenjing" last="Yao">Wenjing Yao</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China/Bamboo Research Institute, Nanjing Forestry University, 159 Longpan Road, Nanjing, 210037, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Dawei" sort="Zhang, Dawei" uniqKey="Zhang D" first="Dawei" last="Zhang">Dawei Zhang</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Boru" sort="Zhou, Boru" uniqKey="Zhou B" first="Boru" last="Zhou">Boru Zhou</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China. 896099005@qq.com.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Jianping" sort="Wang, Jianping" uniqKey="Wang J" first="Jianping" last="Wang">Jianping Wang</name><affiliation><nlm:affiliation>Department of Agronomy, University of Florida, 2033 Mowry Road, Gainesville, FL, 32610, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Renhua" sort="Li, Renhua" uniqKey="Li R" first="Renhua" last="Li">Renhua Li</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.</nlm:affiliation></affiliation></author><author><name sortKey="Jiang, Tingbo" sort="Jiang, Tingbo" uniqKey="Jiang T" first="Tingbo" last="Jiang">Tingbo Jiang</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China. tbjiang@yahoo.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">BMC plant biology</title><idno type="eISSN">1471-2229</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (metabolism)</term><term>Cellulose (metabolism)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Lignin (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (genetics)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term><term>Transcription Factors (genetics)</term><term>Transcription Factors (metabolism)</term><term>Wood (metabolism)</term><term>Xylem (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulose</term><term>Lignin</term><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Plants, Genetically Modified</term><term>Tobacco</term><term>Wood</term><term>Xylem</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>NAC (NAM/ATAF/CUC) is one of the largest plant-specific transcription factor (TF) families known to play significant roles in wood formation. Acting as master gene regulators, a few NAC genes can activate secondary wall biosynthesis during wood formation in woody plants.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>In the present study, firstly, we screened 110 differentially expressed NAC genes in the leaves, stems, and roots of di-haploid Populus simonii×P. nigra by RNA-Seq. Then we identified a nucleus-targeted gene, NAC15 gene, which was one of the highly expressed genes in the stem among 110 NAC family members. Thirdly, we conducted expression pattern analysis of NAC15 gene, and observed NAC15 gene was most highly expressed in the xylem by RT-qPCR. Moreover, we transferred NAC15 gene into tobacco and obtained 12 transgenic lines overexpressing NAC15 gene (TLs). And the relative higher content of hemicellulose, cellulose and lignin was observed in the TLs compared to the control lines containing empty vector (CLs). It also showed darker staining in the culms of the TLs with phloroglucinol staining, compared to the CLs. Furthermore, the relative expression level of a few lignin- and cellulose-related genes was significantly higher in the TLs than that in the CLs.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>The overall results indicated that NAC15 gene is highly expressed in the xylem of poplar and may be a potential candidate gene playing an important role in wood formation in transgenic tobacco.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31914923</PMID><DateCompleted><Year>2020</Year><Month>06</Month><Day>05</Day></DateCompleted><DateRevised><Year>2020</Year><Month>06</Month><Day>05</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2229</ISSN><JournalIssue CitedMedium="Internet"><Volume>20</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>Jan</Month><Day>08</Day></PubDate></JournalIssue><Title>BMC plant biology</Title><ISOAbbreviation>BMC Plant Biol</ISOAbbreviation></Journal><ArticleTitle>Over-expression of poplar NAC15 gene enhances wood formation in transgenic tobacco.</ArticleTitle><Pagination><MedlinePgn>12</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12870-019-2191-2</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">NAC (NAM/ATAF/CUC) is one of the largest plant-specific transcription factor (TF) families known to play significant roles in wood formation. Acting as master gene regulators, a few NAC genes can activate secondary wall biosynthesis during wood formation in woody plants.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">In the present study, firstly, we screened 110 differentially expressed NAC genes in the leaves, stems, and roots of di-haploid Populus simonii×P. nigra by RNA-Seq. Then we identified a nucleus-targeted gene, NAC15 gene, which was one of the highly expressed genes in the stem among 110 NAC family members. Thirdly, we conducted expression pattern analysis of NAC15 gene, and observed NAC15 gene was most highly expressed in the xylem by RT-qPCR. Moreover, we transferred NAC15 gene into tobacco and obtained 12 transgenic lines overexpressing NAC15 gene (TLs). And the relative higher content of hemicellulose, cellulose and lignin was observed in the TLs compared to the control lines containing empty vector (CLs). It also showed darker staining in the culms of the TLs with phloroglucinol staining, compared to the CLs. Furthermore, the relative expression level of a few lignin- and cellulose-related genes was significantly higher in the TLs than that in the CLs.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The overall results indicated that NAC15 gene is highly expressed in the xylem of poplar and may be a potential candidate gene playing an important role in wood formation in transgenic tobacco.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yao</LastName><ForeName>Wenjing</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China/Bamboo Research Institute, Nanjing Forestry University, 159 Longpan Road, Nanjing, 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Dawei</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Boru</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China. 896099005@qq.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jianping</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Agronomy, University of Florida, 2033 Mowry Road, Gainesville, FL, 32610, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Renhua</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Tingbo</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China. tbjiang@yahoo.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>2572018CL03</GrantID><Agency>Fundamental Research Funds for the Central Universities</Agency><Country></Country></Grant><Grant><GrantID>BK20190748</GrantID><Agency>Natural Science Foundation of Jiangsu Province (CN)</Agency><Country></Country></Grant><Grant><GrantID>B16010</GrantID><Agency>111 Project</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate 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UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052584" 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