Overexpression of Populus transcription factor PtrTALE12 increases axillary shoot development by regulating WUSCHEL expression.
Identifieur interne : 000298 ( Main/Corpus ); précédent : 000297; suivant : 000299Overexpression of Populus transcription factor PtrTALE12 increases axillary shoot development by regulating WUSCHEL expression.
Auteurs : So-Young Bae ; Min-Ha Kim ; Jin-Seong Cho ; Eung-Jun Park ; Hyoshin Lee ; Jeong-Hoe Kim ; Jae-Heung KoSource :
- Tree physiology [ 1758-4469 ] ; 2020.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Arabidopsis Proteins, Homeodomain Proteins, Transcription Factors.
- genetics : Meristem, Populus.
- Gene Expression Regulation, Plant.
Abstract
The TALE (Three Amino acid Loop Extension) transcription factor family has been shown to control meristem formation and organogenesis in plants. To understand the functional roles of the TALE family in woody perennials, each of the TALE members of Populus trichocarpa was overexpressed in Arabidopsis as a proxy. Among them, the overexpression of PtrTALE12 (i.e., 35S::PtrTALE12) resulted in a dramatic increase of axillary shoot development with early flowering. Interestingly, expression of WUSCHEL (WUS), a central regulator of both apical and axillary meristem formation, was significantly increased in the 35S::PtrTALE12 Arabidopsis plants. Conversely, WUS expression was downregulated in 35S::PtrTALE12-SRDX (short transcriptional repressor domain) plants. Further analysis found that PtrTALE12, expressed preferentially in meristem tissues, directly regulates WUS expression in transient activation assays using Arabidopsis leaf protoplast. Yeast two-hybrid assays showed that PtrTALE12 interacts with SHOOT MERISTEMLESS (STM); however, the interaction does not affect the WUS expression. In addition, expression of both CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) genes was suppressed accordingly for early flowering 35S::PtrTALE12 Arabidopsis. Indeed, transgenic poplars overexpressing PtrTALE12 as well as Arabidopsis plants overexpressing AtBLH11, a close homolog of PtrTALE12, phenocopied the 35S::PtrTALE12 Arabidopsis (i.e., increased axillary shoot development). Taken together, our results suggest that PtrTALE12 functions as a positive regulator of axillary shoot formation in both Arabidopsis and poplar.
DOI: 10.1093/treephys/tpaa062
PubMed: 32420604
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of Plant & Environmental New Resources, Kyung Hee University, 1732 Deogyeong-daero, Yongin 17104, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Park, Eung Jun" sort="Park, Eung Jun" uniqKey="Park E" first="Eung-Jun" last="Park">Eung-Jun Park</name><affiliation><nlm:affiliation>Division of Forest Biotechnology, National Institute of Forest Science, 39 Onjeong-ro, Suwon 16631, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Hyoshin" sort="Lee, Hyoshin" uniqKey="Lee H" first="Hyoshin" last="Lee">Hyoshin Lee</name><affiliation><nlm:affiliation>Division of Forest Biotechnology, National Institute of Forest Science, 39 Onjeong-ro, Suwon 16631, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Jeong Hoe" sort="Kim, Jeong Hoe" uniqKey="Kim J" first="Jeong-Hoe" last="Kim">Jeong-Hoe Kim</name><affiliation><nlm:affiliation>Department of Biology, School of Biological Sciences, Kyungpook National University, Daegu 41566, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Ko, Jae Heung" sort="Ko, Jae Heung" uniqKey="Ko J" first="Jae-Heung" last="Ko">Jae-Heung Ko</name><affiliation><nlm:affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, 1732 Deogyeong-daero, Yongin 17104, Republic of Korea.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32420604</idno><idno type="pmid">32420604</idno><idno type="doi">10.1093/treephys/tpaa062</idno><idno type="wicri:Area/Main/Corpus">000298</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000298</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Overexpression of Populus transcription factor PtrTALE12 increases axillary shoot development by regulating WUSCHEL expression.</title><author><name sortKey="Bae, So Young" sort="Bae, So Young" uniqKey="Bae S" first="So-Young" last="Bae">So-Young Bae</name><affiliation><nlm:affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, 1732 Deogyeong-daero, Yongin 17104, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Min Ha" sort="Kim, Min Ha" uniqKey="Kim M" first="Min-Ha" last="Kim">Min-Ha Kim</name><affiliation><nlm:affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, 1732 Deogyeong-daero, Yongin 17104, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Cho, Jin Seong" sort="Cho, Jin Seong" uniqKey="Cho J" first="Jin-Seong" last="Cho">Jin-Seong Cho</name><affiliation><nlm:affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, 1732 Deogyeong-daero, Yongin 17104, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Park, Eung Jun" sort="Park, Eung Jun" uniqKey="Park E" first="Eung-Jun" last="Park">Eung-Jun Park</name><affiliation><nlm:affiliation>Division of Forest Biotechnology, National Institute of Forest Science, 39 Onjeong-ro, Suwon 16631, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Hyoshin" sort="Lee, Hyoshin" uniqKey="Lee H" first="Hyoshin" last="Lee">Hyoshin Lee</name><affiliation><nlm:affiliation>Division of Forest Biotechnology, National Institute of Forest Science, 39 Onjeong-ro, Suwon 16631, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Jeong Hoe" sort="Kim, Jeong Hoe" uniqKey="Kim J" first="Jeong-Hoe" last="Kim">Jeong-Hoe Kim</name><affiliation><nlm:affiliation>Department of Biology, School of Biological Sciences, Kyungpook National University, Daegu 41566, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Ko, Jae Heung" sort="Ko, Jae Heung" uniqKey="Ko J" first="Jae-Heung" last="Ko">Jae-Heung Ko</name><affiliation><nlm:affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, 1732 Deogyeong-daero, Yongin 17104, Republic of Korea.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis Proteins (genetics)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Homeodomain Proteins (genetics)</term><term>Meristem (genetics)</term><term>Populus (genetics)</term><term>Transcription Factors (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Arabidopsis Proteins</term><term>Homeodomain Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Meristem</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The TALE (Three Amino acid Loop Extension) transcription factor family has been shown to control meristem formation and organogenesis in plants. To understand the functional roles of the TALE family in woody perennials, each of the TALE members of Populus trichocarpa was overexpressed in Arabidopsis as a proxy. Among them, the overexpression of PtrTALE12 (i.e., 35S::PtrTALE12) resulted in a dramatic increase of axillary shoot development with early flowering. Interestingly, expression of WUSCHEL (WUS), a central regulator of both apical and axillary meristem formation, was significantly increased in the 35S::PtrTALE12 Arabidopsis plants. Conversely, WUS expression was downregulated in 35S::PtrTALE12-SRDX (short transcriptional repressor domain) plants. Further analysis found that PtrTALE12, expressed preferentially in meristem tissues, directly regulates WUS expression in transient activation assays using Arabidopsis leaf protoplast. Yeast two-hybrid assays showed that PtrTALE12 interacts with SHOOT MERISTEMLESS (STM); however, the interaction does not affect the WUS expression. In addition, expression of both CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) genes was suppressed accordingly for early flowering 35S::PtrTALE12 Arabidopsis. Indeed, transgenic poplars overexpressing PtrTALE12 as well as Arabidopsis plants overexpressing AtBLH11, a close homolog of PtrTALE12, phenocopied the 35S::PtrTALE12 Arabidopsis (i.e., increased axillary shoot development). Taken together, our results suggest that PtrTALE12 functions as a positive regulator of axillary shoot formation in both Arabidopsis and poplar.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">32420604</PMID><DateCompleted><Year>2020</Year><Month>10</Month><Day>01</Day></DateCompleted><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><Volume>40</Volume><Issue>9</Issue><PubDate><Year>2020</Year><Month>08</Month><Day>29</Day></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Overexpression of Populus transcription factor PtrTALE12 increases axillary shoot development by regulating WUSCHEL expression.</ArticleTitle><Pagination><MedlinePgn>1232-1246</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpaa062</ELocationID><Abstract><AbstractText>The TALE (Three Amino acid Loop Extension) transcription factor family has been shown to control meristem formation and organogenesis in plants. To understand the functional roles of the TALE family in woody perennials, each of the TALE members of Populus trichocarpa was overexpressed in Arabidopsis as a proxy. Among them, the overexpression of PtrTALE12 (i.e., 35S::PtrTALE12) resulted in a dramatic increase of axillary shoot development with early flowering. Interestingly, expression of WUSCHEL (WUS), a central regulator of both apical and axillary meristem formation, was significantly increased in the 35S::PtrTALE12 Arabidopsis plants. Conversely, WUS expression was downregulated in 35S::PtrTALE12-SRDX (short transcriptional repressor domain) plants. Further analysis found that PtrTALE12, expressed preferentially in meristem tissues, directly regulates WUS expression in transient activation assays using Arabidopsis leaf protoplast. Yeast two-hybrid assays showed that PtrTALE12 interacts with SHOOT MERISTEMLESS (STM); however, the interaction does not affect the WUS expression. In addition, expression of both CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) genes was suppressed accordingly for early flowering 35S::PtrTALE12 Arabidopsis. Indeed, transgenic poplars overexpressing PtrTALE12 as well as Arabidopsis plants overexpressing AtBLH11, a close homolog of PtrTALE12, phenocopied the 35S::PtrTALE12 Arabidopsis (i.e., increased axillary shoot development). Taken together, our results suggest that PtrTALE12 functions as a positive regulator of axillary shoot formation in both Arabidopsis and poplar.</AbstractText><CopyrightInformation>© The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bae</LastName><ForeName>So-Young</ForeName><Initials>SY</Initials><AffiliationInfo><Affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, 1732 Deogyeong-daero, Yongin 17104, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Min-Ha</ForeName><Initials>MH</Initials><AffiliationInfo><Affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, 1732 Deogyeong-daero, Yongin 17104, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cho</LastName><ForeName>Jin-Seong</ForeName><Initials>JS</Initials><AffiliationInfo><Affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, 1732 Deogyeong-daero, Yongin 17104, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Eung-Jun</ForeName><Initials>EJ</Initials><AffiliationInfo><Affiliation>Division of Forest Biotechnology, National Institute of Forest Science, 39 Onjeong-ro, Suwon 16631, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Hyoshin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Division of Forest Biotechnology, National Institute of Forest Science, 39 Onjeong-ro, Suwon 16631, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Jeong-Hoe</ForeName><Initials>JH</Initials><AffiliationInfo><Affiliation>Department of Biology, School of Biological Sciences, Kyungpook National University, Daegu 41566, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ko</LastName><ForeName>Jae-Heung</ForeName><Initials>JH</Initials><AffiliationInfo><Affiliation>Department of Plant & Environmental New Resources, Kyung Hee University, 1732 Deogyeong-daero, Yongin 17104, Republic of Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029681">Arabidopsis Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018398">Homeodomain Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D029681" MajorTopicYN="N">Arabidopsis Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018398" MajorTopicYN="N">Homeodomain Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018519" MajorTopicYN="N">Meristem</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">PtrTALE12</Keyword><Keyword MajorTopicYN="Y">WUSCHEL</Keyword><Keyword MajorTopicYN="Y">axillary shoot development</Keyword><Keyword MajorTopicYN="Y">poplar</Keyword><Keyword MajorTopicYN="Y">tree architecture</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>12</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>03</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>05</Month><Day>13</Day></PubMedPubDate><PubMedPubDate 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