Ectopic expression of a poplar gene NAC13 confers enhanced tolerance to salinity stress in transgenic Nicotiana tabacum.
Identifieur interne : 000204 ( Main/Corpus ); précédent : 000203; suivant : 000205Ectopic expression of a poplar gene NAC13 confers enhanced tolerance to salinity stress in transgenic Nicotiana tabacum.
Auteurs : Zihan Cheng ; Xuemei Zhang ; Kai Zhao ; Boru Zhou ; Tingbo JiangSource :
- Journal of plant research [ 1618-0860 ] ; 2020.
English descriptors
- KwdEn :
- Ectopic Gene Expression (MeSH), Gene Expression Regulation, Plant (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (metabolism), Stress, Physiological (genetics), Tobacco (genetics), Tobacco (metabolism).
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Plant Proteins.
- genetics : Plants, Genetically Modified, Populus, Stress, Physiological, Tobacco.
- metabolism : Plants, Genetically Modified, Populus, Tobacco.
- Ectopic Gene Expression, Gene Expression Regulation, Plant.
Abstract
NACs are one of the major transcription factor families in plants which play an important role in plant growth and development, as well as in adverse stress responses. In this study, we cloned a salt-inducible NAC transcription factor gene (NAC13) from a poplar variety 84K, followed by transforming it into both Nicotiana tabacum and Arabidopsis thaliana. Stable expression analysis of 35S::NAC13-GFP fusion protein in Arabidopsis indicated that NAC13 protein was localized to the nucleus. We also obtained five transgenic tobacco lines. Evidence from morphological and physiological characterization and salt treatment analyses indicated that in the transgenic tobacco the salt tolerance was enhanced, suggesting that NAC13 gene may function as a positive regulator in tobacco responses to salt stress.
DOI: 10.1007/s10265-020-01213-z
PubMed: 32643054
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pubmed:32643054Le document en format XML
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Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Boru" sort="Zhou, Boru" uniqKey="Zhou B" first="Boru" last="Zhou">Boru Zhou</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China. boruzhou@yahoo.com.</nlm:affiliation></affiliation></author><author><name sortKey="Jiang, Tingbo" sort="Jiang, Tingbo" uniqKey="Jiang T" first="Tingbo" last="Jiang">Tingbo Jiang</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China. tbjiang@yahoo.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32643054</idno><idno type="pmid">32643054</idno><idno 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China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhao, Kai" sort="Zhao, Kai" uniqKey="Zhao K" first="Kai" last="Zhao">Kai Zhao</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Boru" sort="Zhou, Boru" uniqKey="Zhou B" first="Boru" last="Zhou">Boru Zhou</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China. boruzhou@yahoo.com.</nlm:affiliation></affiliation></author><author><name sortKey="Jiang, Tingbo" sort="Jiang, Tingbo" uniqKey="Jiang T" first="Tingbo" last="Jiang">Tingbo Jiang</name><affiliation><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China. tbjiang@yahoo.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of plant research</title><idno type="eISSN">1618-0860</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ectopic Gene Expression (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Stress, Physiological (genetics)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term><term>Stress, Physiological</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Ectopic Gene Expression</term><term>Gene Expression Regulation, Plant</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">NACs are one of the major transcription factor families in plants which play an important role in plant growth and development, as well as in adverse stress responses. In this study, we cloned a salt-inducible NAC transcription factor gene (NAC13) from a poplar variety 84K, followed by transforming it into both Nicotiana tabacum and Arabidopsis thaliana. Stable expression analysis of 35S::NAC13-GFP fusion protein in Arabidopsis indicated that NAC13 protein was localized to the nucleus. We also obtained five transgenic tobacco lines. Evidence from morphological and physiological characterization and salt treatment analyses indicated that in the transgenic tobacco the salt tolerance was enhanced, suggesting that NAC13 gene may function as a positive regulator in tobacco responses to salt stress.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">32643054</PMID><DateCompleted><Year>2020</Year><Month>10</Month><Day>27</Day></DateCompleted><DateRevised><Year>2020</Year><Month>10</Month><Day>27</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-0860</ISSN><JournalIssue CitedMedium="Internet"><Volume>133</Volume><Issue>5</Issue><PubDate><Year>2020</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Journal of plant research</Title><ISOAbbreviation>J Plant Res</ISOAbbreviation></Journal><ArticleTitle>Ectopic expression of a poplar gene NAC13 confers enhanced tolerance to salinity stress in transgenic Nicotiana tabacum.</ArticleTitle><Pagination><MedlinePgn>727-737</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10265-020-01213-z</ELocationID><Abstract><AbstractText>NACs are one of the major transcription factor families in plants which play an important role in plant growth and development, as well as in adverse stress responses. In this study, we cloned a salt-inducible NAC transcription factor gene (NAC13) from a poplar variety 84K, followed by transforming it into both Nicotiana tabacum and Arabidopsis thaliana. Stable expression analysis of 35S::NAC13-GFP fusion protein in Arabidopsis indicated that NAC13 protein was localized to the nucleus. We also obtained five transgenic tobacco lines. Evidence from morphological and physiological characterization and salt treatment analyses indicated that in the transgenic tobacco the salt tolerance was enhanced, suggesting that NAC13 gene may function as a positive regulator in tobacco responses to salt stress.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Zihan</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Xuemei</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Kai</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Boru</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China. boruzhou@yahoo.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Tingbo</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China. tbjiang@yahoo.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>2018ZX08020002</GrantID><Agency>National Key Program on Transgenic Research</Agency><Country></Country></Grant><Grant><GrantID>B16010</GrantID><Agency>111 project</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>J Plant Res</MedlineTA><NlmUniqueID>9887853</NlmUniqueID><ISSNLinking>0918-9440</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000066630" MajorTopicYN="Y">Ectopic Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="Y">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="Y">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Ectopic expression</Keyword><Keyword MajorTopicYN="N">NAC13 gene</Keyword><Keyword MajorTopicYN="N">Salt tolerance</Keyword><Keyword MajorTopicYN="N">Transcription factor</Keyword><Keyword MajorTopicYN="N">Transgenic tobacco</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>02</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>06</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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