Metabolic profiling and gene expression analysis provides insights into flavonoid and anthocyanin metabolism in poplar.
Identifieur interne : 000014 ( Main/Corpus ); précédent : 000013; suivant : 000015Metabolic profiling and gene expression analysis provides insights into flavonoid and anthocyanin metabolism in poplar.
Auteurs : Ruru Tian ; Qianqian Li ; Shupei Rao ; Aike Wang ; Hechen Zhang ; Liangsheng Wang ; Yue Li ; Jinhuan ChenSource :
- Tree physiology [ 1758-4469 ] ; 2020.
Abstract
Poplar, a woody perennial model, is a common and widespread tree genus. We cultivated two red leaf poplar varieties from bud mutation of Populus sp. Linn. '2025' (also known as Zhonglin 2025, L2025 for shot): Populus deltoides varieties with bright red leaves (LHY) and completely red leaves (QHY). After measuring total contents of flavonoid, anthocyanin, chlorophyll, and carotenoid metabolites, a liquid chromatography-electrospray ionization-tandem mass spectrometry system was used for the relative quantification of widely targeted metabolites in leaves of three poplar varieties. A total of 210 flavonoid metabolites (89 flavones, 40 flavonols, 25 flavanones, 18 anthocyanins, 16 isoflavones, 7 dihydroflavonols, 7 chalcones, 5 proanthocyanidins, and 3 other flavonoid metabolites) were identified. Compared with L2025, 48 and 8 flavonoids were more and less abundant, respectively, in LHY, whereas 51 and 9 flavonoids were more and less abundant in QHY, respectively. On the basis of a comprehensive analysis of the metabolic network, gene expression levels were analyzed by deep-sequencing to screen for potential reference genes for the red leaves. Most phenylpropanoid biosynthesis pathway involved genes were differentially expressed among the examined varieties. Gene expression analysis also revealed several potential anthocyanin biosynthesis regulators including three MYB genes. The study results provide new insights into poplar flavonoid metabolites and represent the theoretical basis for future studies on leaf coloration in this model tree species.
DOI: 10.1093/treephys/tpaa152
PubMed: 33169130
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Metabolic profiling and gene expression analysis provides insights into flavonoid and anthocyanin metabolism in poplar.</title><author><name sortKey="Tian, Ruru" sort="Tian, Ruru" uniqKey="Tian R" first="Ruru" last="Tian">Ruru Tian</name><affiliation><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Qianqian" sort="Li, Qianqian" uniqKey="Li Q" first="Qianqian" last="Li">Qianqian Li</name><affiliation><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation></author><author><name sortKey="Rao, Shupei" sort="Rao, Shupei" uniqKey="Rao S" first="Shupei" last="Rao">Shupei Rao</name><affiliation><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Aike" sort="Wang, Aike" uniqKey="Wang A" first="Aike" last="Wang">Aike Wang</name><affiliation><nlm:affiliation>Yucheng Institute of Agricultural Sciences, Shangqiu, Henan 476000.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Shangqiu Zhongxing Seedling Planting Co., Ltd, Shangqiu, Henan 476000, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Hechen" sort="Zhang, Hechen" uniqKey="Zhang H" first="Hechen" last="Zhang">Hechen Zhang</name><affiliation><nlm:affiliation>Horticultural Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Liangsheng" sort="Wang, Liangsheng" uniqKey="Wang L" first="Liangsheng" last="Wang">Liangsheng Wang</name><affiliation><nlm:affiliation>Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese academy of sciences, Beijing 100093, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Yue" sort="Li, Yue" uniqKey="Li Y" first="Yue" last="Li">Yue Li</name><affiliation><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Jinhuan" sort="Chen, Jinhuan" uniqKey="Chen J" first="Jinhuan" last="Chen">Jinhuan Chen</name><affiliation><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33169130</idno><idno type="pmid">33169130</idno><idno type="doi">10.1093/treephys/tpaa152</idno><idno type="wicri:Area/Main/Corpus">000014</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000014</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Metabolic profiling and gene expression analysis provides insights into flavonoid and anthocyanin metabolism in poplar.</title><author><name sortKey="Tian, Ruru" sort="Tian, Ruru" uniqKey="Tian R" first="Ruru" last="Tian">Ruru Tian</name><affiliation><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Qianqian" sort="Li, Qianqian" uniqKey="Li Q" first="Qianqian" last="Li">Qianqian Li</name><affiliation><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation></author><author><name sortKey="Rao, Shupei" sort="Rao, Shupei" uniqKey="Rao S" first="Shupei" last="Rao">Shupei Rao</name><affiliation><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Aike" sort="Wang, Aike" uniqKey="Wang A" first="Aike" last="Wang">Aike Wang</name><affiliation><nlm:affiliation>Yucheng Institute of Agricultural Sciences, Shangqiu, Henan 476000.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Shangqiu Zhongxing Seedling Planting Co., Ltd, Shangqiu, Henan 476000, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Hechen" sort="Zhang, Hechen" uniqKey="Zhang H" first="Hechen" last="Zhang">Hechen Zhang</name><affiliation><nlm:affiliation>Horticultural Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Liangsheng" sort="Wang, Liangsheng" uniqKey="Wang L" first="Liangsheng" last="Wang">Liangsheng Wang</name><affiliation><nlm:affiliation>Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese academy of sciences, Beijing 100093, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Yue" sort="Li, Yue" uniqKey="Li Y" first="Yue" last="Li">Yue Li</name><affiliation><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Jinhuan" sort="Chen, Jinhuan" uniqKey="Chen J" first="Jinhuan" last="Chen">Jinhuan Chen</name><affiliation><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Poplar, a woody perennial model, is a common and widespread tree genus. We cultivated two red leaf poplar varieties from bud mutation of Populus sp. Linn. '2025' (also known as Zhonglin 2025, L2025 for shot): Populus deltoides varieties with bright red leaves (LHY) and completely red leaves (QHY). After measuring total contents of flavonoid, anthocyanin, chlorophyll, and carotenoid metabolites, a liquid chromatography-electrospray ionization-tandem mass spectrometry system was used for the relative quantification of widely targeted metabolites in leaves of three poplar varieties. A total of 210 flavonoid metabolites (89 flavones, 40 flavonols, 25 flavanones, 18 anthocyanins, 16 isoflavones, 7 dihydroflavonols, 7 chalcones, 5 proanthocyanidins, and 3 other flavonoid metabolites) were identified. Compared with L2025, 48 and 8 flavonoids were more and less abundant, respectively, in LHY, whereas 51 and 9 flavonoids were more and less abundant in QHY, respectively. On the basis of a comprehensive analysis of the metabolic network, gene expression levels were analyzed by deep-sequencing to screen for potential reference genes for the red leaves. Most phenylpropanoid biosynthesis pathway involved genes were differentially expressed among the examined varieties. Gene expression analysis also revealed several potential anthocyanin biosynthesis regulators including three MYB genes. The study results provide new insights into poplar flavonoid metabolites and represent the theoretical basis for future studies on leaf coloration in this model tree species.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33169130</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Nov</Month><Day>09</Day></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Metabolic profiling and gene expression analysis provides insights into flavonoid and anthocyanin metabolism in poplar.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">tpaa152</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpaa152</ELocationID><Abstract><AbstractText>Poplar, a woody perennial model, is a common and widespread tree genus. We cultivated two red leaf poplar varieties from bud mutation of Populus sp. Linn. '2025' (also known as Zhonglin 2025, L2025 for shot): Populus deltoides varieties with bright red leaves (LHY) and completely red leaves (QHY). After measuring total contents of flavonoid, anthocyanin, chlorophyll, and carotenoid metabolites, a liquid chromatography-electrospray ionization-tandem mass spectrometry system was used for the relative quantification of widely targeted metabolites in leaves of three poplar varieties. A total of 210 flavonoid metabolites (89 flavones, 40 flavonols, 25 flavanones, 18 anthocyanins, 16 isoflavones, 7 dihydroflavonols, 7 chalcones, 5 proanthocyanidins, and 3 other flavonoid metabolites) were identified. Compared with L2025, 48 and 8 flavonoids were more and less abundant, respectively, in LHY, whereas 51 and 9 flavonoids were more and less abundant in QHY, respectively. On the basis of a comprehensive analysis of the metabolic network, gene expression levels were analyzed by deep-sequencing to screen for potential reference genes for the red leaves. Most phenylpropanoid biosynthesis pathway involved genes were differentially expressed among the examined varieties. Gene expression analysis also revealed several potential anthocyanin biosynthesis regulators including three MYB genes. The study results provide new insights into poplar flavonoid metabolites and represent the theoretical basis for future studies on leaf coloration in this model tree species.</AbstractText><CopyrightInformation>© The Author(s) 2020. Published by Oxford University Press. All rights reserved. Forpermissions, please e-mail: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tian</LastName><ForeName>Ruru</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Qianqian</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rao</LastName><ForeName>Shupei</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Aike</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Yucheng Institute of Agricultural Sciences, Shangqiu, Henan 476000.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Shangqiu Zhongxing Seedling Planting Co., Ltd, Shangqiu, Henan 476000, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Hechen</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Horticultural Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Liangsheng</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese academy of sciences, Beijing 100093, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yue</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Jinhuan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Biological Sciences and technology, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">anthocyanin</Keyword><Keyword MajorTopicYN="N">flavonoids</Keyword><Keyword MajorTopicYN="N">gene expression</Keyword><Keyword MajorTopicYN="N">leaf coloration</Keyword><Keyword MajorTopicYN="N">metabolic profiling</Keyword><Keyword MajorTopicYN="N">poplar</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>03</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>07</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>10</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>10</Day><Hour>5</Hour><Minute>46</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33169130</ArticleId><ArticleId IdType="pii">5963549</ArticleId><ArticleId 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