Candidate Effector Proteins of the Rust Pathogen Melampsora larici-populina Target Diverse Plant Cell Compartments.
Identifieur interne : 000039 ( Main/Exploration ); précédent : 000038; suivant : 000040Candidate Effector Proteins of the Rust Pathogen Melampsora larici-populina Target Diverse Plant Cell Compartments.
Auteurs : Benjamin Petre [Royaume-Uni, France] ; Diane G O. Saunders [Royaume-Uni] ; Jan Sklenar [Royaume-Uni] ; Cécile Lorrain [Royaume-Uni, France] ; Joe Win [Royaume-Uni] ; Sébastien Duplessis [France] ; Sophien Kamoun [Royaume-Uni]Source :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2015.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes (MeSH), Basidiomycota (génétique), Basidiomycota (physiologie), Expression des gènes (MeSH), Feuilles de plante (microbiologie), Gènes rapporteurs (MeSH), Génome fongique (génétique), Maladies des plantes (microbiologie), Populus (microbiologie), Protéines de fusion recombinantes (MeSH), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Séquençage par oligonucléotides en batterie (MeSH), Tabac (cytologie), Tabac (microbiologie), Transgènes (MeSH), Transport des protéines (MeSH).
- MESH :
- cytologie : Tabac.
- génétique : Basidiomycota, Génome fongique, Protéines fongiques, Protéines végétales.
- microbiologie : Feuilles de plante, Maladies des plantes, Populus, Tabac.
- métabolisme : Protéines fongiques, Protéines végétales.
- physiologie : Basidiomycota.
- Analyse de profil d'expression de gènes, Expression des gènes, Gènes rapporteurs, Protéines de fusion recombinantes, Séquençage par oligonucléotides en batterie, Transgènes, Transport des protéines.
English descriptors
- KwdEn :
- Basidiomycota (genetics), Basidiomycota (physiology), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression (MeSH), Gene Expression Profiling (MeSH), Genes, Reporter (MeSH), Genome, Fungal (genetics), Oligonucleotide Array Sequence Analysis (MeSH), Plant Diseases (microbiology), Plant Leaves (microbiology), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (microbiology), Protein Transport (MeSH), Recombinant Fusion Proteins (MeSH), Tobacco (cytology), Tobacco (microbiology), Transgenes (MeSH).
- MESH :
- chemical , genetics : Fungal Proteins, Plant Proteins.
- cytology : Tobacco.
- genetics : Basidiomycota, Genome, Fungal.
- chemical , metabolism : Fungal Proteins, Plant Proteins.
- microbiology : Plant Diseases, Plant Leaves, Populus, Tobacco.
- physiology : Basidiomycota.
- Gene Expression, Gene Expression Profiling, Genes, Reporter, Oligonucleotide Array Sequence Analysis, Protein Transport, Recombinant Fusion Proteins, Transgenes.
Abstract
Rust fungi are devastating crop pathogens that deliver effector proteins into infected tissues to modulate plant functions and promote parasitic growth. The genome of the poplar leaf rust fungus Melampsora larici-populina revealed a large catalog of secreted proteins, some of which have been considered candidate effectors. Unraveling how these proteins function in host cells is a key to understanding pathogenicity mechanisms and developing resistant plants. In this study, we used an effectoromics pipeline to select, clone, and express 20 candidate effectors in Nicotiana benthamiana leaf cells to determine their subcellular localization and identify the plant proteins they interact with. Confocal microscopy revealed that six candidate effectors target the nucleus, nucleoli, chloroplasts, mitochondria, and discrete cellular bodies. We also used coimmunoprecipitation (coIP) and mass spectrometry to identify 606 N. benthamiana proteins that associate with the candidate effectors. Five candidate effectors specifically associated with a small set of plant proteins that may represent biologically relevant interactors. We confirmed the interaction between the candidate effector MLP124017 and TOPLESS-related protein 4 from poplar by in planta coIP. Altogether, our data enable us to validate effector proteins from M. larici-populina and reveal that these proteins may target multiple compartments and processes in plant cells. It also shows that N. benthamiana can be a powerful heterologous system to study effectors of obligate biotrophic pathogens.
DOI: 10.1094/MPMI-01-15-0003-R
PubMed: 25650830
Affiliations:
- France, Royaume-Uni
- Grand Est, Lorraine (région)
- Champenoux, Vandœuvre-lès-Nancy
- Université de Lorraine
Links toward previous steps (curation, corpus...)
Le document en format XML
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Norwich</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>2 INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, 54280 Champenoux, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>2 INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, 54280 Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>3 Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, 54506 Vandoeuvre-lès-Nancy, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>3 Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, 54506 Vandoeuvre-lès-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Saunders, Diane G O" sort="Saunders, Diane G O" uniqKey="Saunders D" first="Diane G O" last="Saunders">Diane G O. Saunders</name><affiliation wicri:level="1"><nlm:affiliation>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, U.K.;</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country><wicri:regionArea>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich</wicri:regionArea><wicri:noRegion>NR4 7UH Norwich</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>4 The Genome Analysis Centre, Norwich Research Park, NR4 7UH Norwich, U.K.;</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country><wicri:regionArea>4 The Genome Analysis Centre, Norwich Research Park, NR4 7UH Norwich</wicri:regionArea><wicri:noRegion>NR4 7UH Norwich</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>5 The John Innes Centre, Norwich Research Park, NR4 7UH Norwich, U.K.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>5 The John Innes Centre, Norwich Research Park, NR4 7UH Norwich</wicri:regionArea><wicri:noRegion>NR4 7UH Norwich</wicri:noRegion></affiliation></author><author><name sortKey="Sklenar, Jan" sort="Sklenar, Jan" uniqKey="Sklenar J" first="Jan" last="Sklenar">Jan Sklenar</name><affiliation wicri:level="1"><nlm:affiliation>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, U.K.;</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country><wicri:regionArea>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich</wicri:regionArea><wicri:noRegion>NR4 7UH Norwich</wicri:noRegion></affiliation></author><author><name sortKey="Lorrain, Cecile" sort="Lorrain, Cecile" uniqKey="Lorrain C" first="Cécile" last="Lorrain">Cécile Lorrain</name><affiliation wicri:level="1"><nlm:affiliation>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, U.K.;</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country><wicri:regionArea>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich</wicri:regionArea><wicri:noRegion>NR4 7UH Norwich</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>2 INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, 54280 Champenoux, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>2 INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, 54280 Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>3 Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, 54506 Vandoeuvre-lès-Nancy, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>3 Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, 54506 Vandoeuvre-lès-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Win, Joe" sort="Win, Joe" uniqKey="Win J" first="Joe" last="Win">Joe Win</name><affiliation wicri:level="1"><nlm:affiliation>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, U.K.;</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country><wicri:regionArea>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich</wicri:regionArea><wicri:noRegion>NR4 7UH Norwich</wicri:noRegion></affiliation></author><author><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><affiliation wicri:level="3"><nlm:affiliation>2 INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, 54280 Champenoux, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>2 INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, 54280 Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>3 Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, 54506 Vandoeuvre-lès-Nancy, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>3 Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, 54506 Vandoeuvre-lès-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Kamoun, Sophien" sort="Kamoun, Sophien" uniqKey="Kamoun S" first="Sophien" last="Kamoun">Sophien Kamoun</name><affiliation wicri:level="1"><nlm:affiliation>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, U.K.;</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country><wicri:regionArea>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich</wicri:regionArea><wicri:noRegion>NR4 7UH Norwich</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (genetics)</term><term>Basidiomycota (physiology)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Gene Expression (MeSH)</term><term>Gene Expression Profiling (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Genome, Fungal (genetics)</term><term>Oligonucleotide Array Sequence Analysis (MeSH)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Populus (microbiology)</term><term>Protein Transport (MeSH)</term><term>Recombinant Fusion Proteins (MeSH)</term><term>Tobacco (cytology)</term><term>Tobacco (microbiology)</term><term>Transgenes (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Basidiomycota (génétique)</term><term>Basidiomycota (physiologie)</term><term>Expression des gènes (MeSH)</term><term>Feuilles de plante (microbiologie)</term><term>Gènes rapporteurs (MeSH)</term><term>Génome fongique (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Populus (microbiologie)</term><term>Protéines de fusion recombinantes (MeSH)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Séquençage par oligonucléotides en batterie (MeSH)</term><term>Tabac (cytologie)</term><term>Tabac (microbiologie)</term><term>Transgènes (MeSH)</term><term>Transport des protéines (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term><term>Genome, Fungal</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term><term>Génome fongique</term><term>Protéines fongiques</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term><term>Maladies des plantes</term><term>Populus</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Populus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines fongiques</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression</term><term>Gene Expression Profiling</term><term>Genes, Reporter</term><term>Oligonucleotide Array Sequence Analysis</term><term>Protein Transport</term><term>Recombinant Fusion Proteins</term><term>Transgenes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Expression des gènes</term><term>Gènes rapporteurs</term><term>Protéines de fusion recombinantes</term><term>Séquençage par oligonucléotides en batterie</term><term>Transgènes</term><term>Transport des protéines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Rust fungi are devastating crop pathogens that deliver effector proteins into infected tissues to modulate plant functions and promote parasitic growth. The genome of the poplar leaf rust fungus Melampsora larici-populina revealed a large catalog of secreted proteins, some of which have been considered candidate effectors. Unraveling how these proteins function in host cells is a key to understanding pathogenicity mechanisms and developing resistant plants. In this study, we used an effectoromics pipeline to select, clone, and express 20 candidate effectors in Nicotiana benthamiana leaf cells to determine their subcellular localization and identify the plant proteins they interact with. Confocal microscopy revealed that six candidate effectors target the nucleus, nucleoli, chloroplasts, mitochondria, and discrete cellular bodies. We also used coimmunoprecipitation (coIP) and mass spectrometry to identify 606 N. benthamiana proteins that associate with the candidate effectors. Five candidate effectors specifically associated with a small set of plant proteins that may represent biologically relevant interactors. We confirmed the interaction between the candidate effector MLP124017 and TOPLESS-related protein 4 from poplar by in planta coIP. Altogether, our data enable us to validate effector proteins from M. larici-populina and reveal that these proteins may target multiple compartments and processes in plant cells. It also shows that N. benthamiana can be a powerful heterologous system to study effectors of obligate biotrophic pathogens. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25650830</PMID><DateCompleted><Year>2015</Year><Month>10</Month><Day>16</Day></DateCompleted><DateRevised><Year>2015</Year><Month>06</Month><Day>27</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>28</Volume><Issue>6</Issue><PubDate><Year>2015</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Candidate Effector Proteins of the Rust Pathogen Melampsora larici-populina Target Diverse Plant Cell Compartments.</ArticleTitle><Pagination><MedlinePgn>689-700</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-01-15-0003-R</ELocationID><Abstract><AbstractText>Rust fungi are devastating crop pathogens that deliver effector proteins into infected tissues to modulate plant functions and promote parasitic growth. The genome of the poplar leaf rust fungus Melampsora larici-populina revealed a large catalog of secreted proteins, some of which have been considered candidate effectors. Unraveling how these proteins function in host cells is a key to understanding pathogenicity mechanisms and developing resistant plants. In this study, we used an effectoromics pipeline to select, clone, and express 20 candidate effectors in Nicotiana benthamiana leaf cells to determine their subcellular localization and identify the plant proteins they interact with. Confocal microscopy revealed that six candidate effectors target the nucleus, nucleoli, chloroplasts, mitochondria, and discrete cellular bodies. We also used coimmunoprecipitation (coIP) and mass spectrometry to identify 606 N. benthamiana proteins that associate with the candidate effectors. Five candidate effectors specifically associated with a small set of plant proteins that may represent biologically relevant interactors. We confirmed the interaction between the candidate effector MLP124017 and TOPLESS-related protein 4 from poplar by in planta coIP. Altogether, our data enable us to validate effector proteins from M. larici-populina and reveal that these proteins may target multiple compartments and processes in plant cells. It also shows that N. benthamiana can be a powerful heterologous system to study effectors of obligate biotrophic pathogens. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Petre</LastName><ForeName>Benjamin</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, U.K.;</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>2 INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, 54280 Champenoux, France;</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>3 Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, 54506 Vandoeuvre-lès-Nancy, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saunders</LastName><ForeName>Diane G O</ForeName><Initials>DG</Initials><AffiliationInfo><Affiliation>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, U.K.;</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>4 The Genome Analysis Centre, Norwich Research Park, NR4 7UH Norwich, U.K.;</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>5 The John Innes Centre, Norwich Research Park, NR4 7UH Norwich, U.K.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sklenar</LastName><ForeName>Jan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, U.K.;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lorrain</LastName><ForeName>Cécile</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, U.K.;</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>2 INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, 54280 Champenoux, France;</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>3 Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, 54506 Vandoeuvre-lès-Nancy, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Win</LastName><ForeName>Joe</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, U.K.;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Duplessis</LastName><ForeName>Sébastien</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>2 INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, 54280 Champenoux, France;</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>3 Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, 54506 Vandoeuvre-lès-Nancy, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kamoun</LastName><ForeName>Sophien</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>1 The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, U.K.;</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>Biotechnology and Biological Sciences Research Council</Agency><Country>United Kingdom</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016681" MajorTopicYN="N">Genome, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020411" MajorTopicYN="N">Oligonucleotide Array Sequence Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021381" MajorTopicYN="N">Protein Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="N">Transgenes</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>2</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>2</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>10</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25650830</ArticleId><ArticleId IdType="doi">10.1094/MPMI-01-15-0003-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li><li>Royaume-Uni</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Champenoux</li><li>Vandœuvre-lès-Nancy</li></settlement><orgName><li>Université de Lorraine</li></orgName></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Petre, Benjamin" sort="Petre, Benjamin" uniqKey="Petre B" first="Benjamin" last="Petre">Benjamin Petre</name></noRegion><name sortKey="Kamoun, Sophien" sort="Kamoun, Sophien" uniqKey="Kamoun S" first="Sophien" last="Kamoun">Sophien Kamoun</name><name sortKey="Lorrain, Cecile" sort="Lorrain, Cecile" uniqKey="Lorrain C" first="Cécile" last="Lorrain">Cécile Lorrain</name><name sortKey="Saunders, Diane G O" sort="Saunders, Diane G O" uniqKey="Saunders D" first="Diane G O" last="Saunders">Diane G O. Saunders</name><name sortKey="Saunders, Diane G O" sort="Saunders, Diane G O" uniqKey="Saunders D" first="Diane G O" last="Saunders">Diane G O. Saunders</name><name sortKey="Saunders, Diane G O" sort="Saunders, Diane G O" uniqKey="Saunders D" first="Diane G O" last="Saunders">Diane G O. Saunders</name><name sortKey="Sklenar, Jan" sort="Sklenar, Jan" uniqKey="Sklenar J" first="Jan" last="Sklenar">Jan Sklenar</name><name sortKey="Win, Joe" sort="Win, Joe" uniqKey="Win J" first="Joe" last="Win">Joe Win</name></country><country name="France"><region name="Grand Est"><name sortKey="Petre, Benjamin" sort="Petre, Benjamin" uniqKey="Petre B" first="Benjamin" last="Petre">Benjamin Petre</name></region><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><name sortKey="Lorrain, Cecile" sort="Lorrain, Cecile" uniqKey="Lorrain C" first="Cécile" last="Lorrain">Cécile Lorrain</name><name sortKey="Lorrain, Cecile" sort="Lorrain, Cecile" uniqKey="Lorrain C" first="Cécile" last="Lorrain">Cécile Lorrain</name><name sortKey="Petre, Benjamin" sort="Petre, Benjamin" uniqKey="Petre B" first="Benjamin" last="Petre">Benjamin Petre</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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