Metabolic and proteomic analysis of nitrogen metabolism mechanisms involved in the sugarcane - Fusarium verticillioides interaction.
Identifieur interne : 000213 ( Main/Corpus ); précédent : 000212; suivant : 000214Metabolic and proteomic analysis of nitrogen metabolism mechanisms involved in the sugarcane - Fusarium verticillioides interaction.
Auteurs : Zeping Wang ; Qian Song ; Liang Shuai ; Reemon Htun ; Mukesh Kumar Malviya ; Yijie Li ; Qiang Liang ; Gemin Zhang ; Muqing Zhang ; Fengjue ZhouSource :
- Journal of plant physiology [ 1618-1328 ] ; 2020.
Abstract
Pokkah boeng disease (PBD) is a foliar disease causing severe losses in sugarcane crop production. Research into resistance mechanisms against the causal agent, Fusarium verticillioides, is particularly important for farmers and researchers. This work based on the comprehensive analysis of metabolic, proteomic, and bioinformatics data on nitrogen (N) metabolism, which revealed that this biosynthetic reactions was closely related to resistance mechanisms in the sugarcane- F. verticillioides interaction. Our results suggested that pathogen infection reduced the suppression of nitrate reductase (NR) activity, reducing ammonium nitrogen (NH4+-N) and nitrate nitrogen (NO3--N) assimilation, which reduces glutamine synthetase (GS), glutamate synthetase (GOGAT) activity and polynucleotide synthesis and promotes RNA degradation, resulting in a decrease in ribosome levels and protein synthesis. Cysteine was found to be associated with the symptoms of PBD, while alanine, lysine, proline, and glutamic acid were found to be involved in protective and regulatory mechanisms as well. Additionally, glutamate played an important role in sugarcane defense against pathogens through the biosynthesis of proline and polyamines. Cyanamide, glutamate, proline, tyrosine, and arachidonic acid metabolism actively participate in resistance and response to stress. C5XPZ6 and C5XCA6 were considered to be critical proteins and key effectors according to this study. In conclusion, we have identified potential proteins and pathways involved in sugarcane resistance to F. verticillioides, revealing new findings that may be useful in the design of future diagnostics or sugarcane protection strategies and providing new insights into the molecular mechanisms of sugarcane-pathogen interactions.
DOI: 10.1016/j.jplph.2020.153207
PubMed: 32593920
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Electronic address: yaheng830619@163.com.</nlm:affiliation></affiliation></author><author><name sortKey="Song, Qian" sort="Song, Qian" uniqKey="Song Q" first="Qian" last="Song">Qian Song</name><affiliation><nlm:affiliation>Flowers Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, 530007, China.</nlm:affiliation></affiliation></author><author><name sortKey="Shuai, Liang" sort="Shuai, Liang" uniqKey="Shuai L" first="Liang" last="Shuai">Liang Shuai</name><affiliation><nlm:affiliation>College of Food and Biological Engineering/Institute of Food Research, Hezhou University, Hezhou, 542899, Guangxi, China.</nlm:affiliation></affiliation></author><author><name sortKey="Htun, Reemon" sort="Htun, Reemon" uniqKey="Htun R" first="Reemon" last="Htun">Reemon Htun</name><affiliation><nlm:affiliation>Department of Biotechnology, Mandalay Technological University, Mandalay, Myanmar.</nlm:affiliation></affiliation></author><author><name sortKey="Malviya, Mukesh Kumar" sort="Malviya, Mukesh Kumar" uniqKey="Malviya M" first="Mukesh Kumar" last="Malviya">Mukesh Kumar Malviya</name><affiliation><nlm:affiliation>Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture. 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Nanning, 530007, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Gemin" sort="Zhang, Gemin" uniqKey="Zhang G" first="Gemin" last="Zhang">Gemin Zhang</name><affiliation><nlm:affiliation>Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture. Nanning, 530007, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Muqing" sort="Zhang, Muqing" uniqKey="Zhang M" first="Muqing" last="Zhang">Muqing Zhang</name><affiliation><nlm:affiliation>College of Agriculture, State Key Laboratory of Conservation and Utilization of Subtropical Agrobioresources, Guangxi University, Nanning, Guangxi, 530004, China. Electronic address: 745784546@qq.com.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Fengjue" sort="Zhou, Fengjue" uniqKey="Zhou F" first="Fengjue" last="Zhou">Fengjue Zhou</name><affiliation><nlm:affiliation>College of Agriculture, State Key Laboratory of Conservation and Utilization of Subtropical Agrobioresources, Guangxi University, Nanning, Guangxi, 530004, China. Electronic address: 2096799690@qq.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32593920</idno><idno type="pmid">32593920</idno><idno type="doi">10.1016/j.jplph.2020.153207</idno><idno type="wicri:Area/Main/Corpus">000213</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000213</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Metabolic and proteomic analysis of nitrogen metabolism mechanisms involved in the sugarcane - Fusarium verticillioides interaction.</title><author><name sortKey="Wang, Zeping" sort="Wang, Zeping" uniqKey="Wang Z" first="Zeping" last="Wang">Zeping Wang</name><affiliation><nlm:affiliation>Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture. Nanning, 530007, China; College of Agriculture, State Key Laboratory of Conservation and Utilization of Subtropical Agrobioresources, Guangxi University, Nanning, Guangxi, 530004, China. Electronic address: yaheng830619@163.com.</nlm:affiliation></affiliation></author><author><name sortKey="Song, Qian" sort="Song, Qian" uniqKey="Song Q" first="Qian" last="Song">Qian Song</name><affiliation><nlm:affiliation>Flowers Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, 530007, China.</nlm:affiliation></affiliation></author><author><name sortKey="Shuai, Liang" sort="Shuai, Liang" uniqKey="Shuai L" first="Liang" last="Shuai">Liang Shuai</name><affiliation><nlm:affiliation>College of Food and Biological Engineering/Institute of Food Research, Hezhou University, Hezhou, 542899, Guangxi, China.</nlm:affiliation></affiliation></author><author><name sortKey="Htun, Reemon" sort="Htun, Reemon" uniqKey="Htun R" first="Reemon" last="Htun">Reemon Htun</name><affiliation><nlm:affiliation>Department of Biotechnology, Mandalay Technological University, Mandalay, Myanmar.</nlm:affiliation></affiliation></author><author><name sortKey="Malviya, Mukesh Kumar" sort="Malviya, Mukesh Kumar" uniqKey="Malviya M" first="Mukesh Kumar" last="Malviya">Mukesh Kumar Malviya</name><affiliation><nlm:affiliation>Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture. Nanning, 530007, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Yijie" sort="Li, Yijie" uniqKey="Li Y" first="Yijie" last="Li">Yijie Li</name><affiliation><nlm:affiliation>Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture. Nanning, 530007, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liang, Qiang" sort="Liang, Qiang" uniqKey="Liang Q" first="Qiang" last="Liang">Qiang Liang</name><affiliation><nlm:affiliation>Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture. Nanning, 530007, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Gemin" sort="Zhang, Gemin" uniqKey="Zhang G" first="Gemin" last="Zhang">Gemin Zhang</name><affiliation><nlm:affiliation>Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture. Nanning, 530007, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Muqing" sort="Zhang, Muqing" uniqKey="Zhang M" first="Muqing" last="Zhang">Muqing Zhang</name><affiliation><nlm:affiliation>College of Agriculture, State Key Laboratory of Conservation and Utilization of Subtropical Agrobioresources, Guangxi University, Nanning, Guangxi, 530004, China. Electronic address: 745784546@qq.com.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Fengjue" sort="Zhou, Fengjue" uniqKey="Zhou F" first="Fengjue" last="Zhou">Fengjue Zhou</name><affiliation><nlm:affiliation>College of Agriculture, State Key Laboratory of Conservation and Utilization of Subtropical Agrobioresources, Guangxi University, Nanning, Guangxi, 530004, China. Electronic address: 2096799690@qq.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of plant physiology</title><idno type="eISSN">1618-1328</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pokkah boeng disease (PBD) is a foliar disease causing severe losses in sugarcane crop production. Research into resistance mechanisms against the causal agent, Fusarium verticillioides, is particularly important for farmers and researchers. This work based on the comprehensive analysis of metabolic, proteomic, and bioinformatics data on nitrogen (N) metabolism, which revealed that this biosynthetic reactions was closely related to resistance mechanisms in the sugarcane- F. verticillioides interaction. Our results suggested that pathogen infection reduced the suppression of nitrate reductase (NR) activity, reducing ammonium nitrogen (NH<sub>4</sub><sup>+</sup>-N) and nitrate nitrogen (NO<sub>3</sub><sup>-</sup>-N) assimilation, which reduces glutamine synthetase (GS), glutamate synthetase (GOGAT) activity and polynucleotide synthesis and promotes RNA degradation, resulting in a decrease in ribosome levels and protein synthesis. Cysteine was found to be associated with the symptoms of PBD, while alanine, lysine, proline, and glutamic acid were found to be involved in protective and regulatory mechanisms as well. Additionally, glutamate played an important role in sugarcane defense against pathogens through the biosynthesis of proline and polyamines. Cyanamide, glutamate, proline, tyrosine, and arachidonic acid metabolism actively participate in resistance and response to stress. C5XPZ6 and C5XCA6 were considered to be critical proteins and key effectors according to this study. In conclusion, we have identified potential proteins and pathways involved in sugarcane resistance to F. verticillioides, revealing new findings that may be useful in the design of future diagnostics or sugarcane protection strategies and providing new insights into the molecular mechanisms of sugarcane-pathogen interactions.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32593920</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-1328</ISSN><JournalIssue CitedMedium="Internet"><Volume>251</Volume><PubDate><Year>2020</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Journal of plant physiology</Title><ISOAbbreviation>J Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Metabolic and proteomic analysis of nitrogen metabolism mechanisms involved in the sugarcane - Fusarium verticillioides interaction.</ArticleTitle><Pagination><MedlinePgn>153207</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0176-1617(20)30097-3</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jplph.2020.153207</ELocationID><Abstract><AbstractText>Pokkah boeng disease (PBD) is a foliar disease causing severe losses in sugarcane crop production. Research into resistance mechanisms against the causal agent, Fusarium verticillioides, is particularly important for farmers and researchers. This work based on the comprehensive analysis of metabolic, proteomic, and bioinformatics data on nitrogen (N) metabolism, which revealed that this biosynthetic reactions was closely related to resistance mechanisms in the sugarcane- F. verticillioides interaction. Our results suggested that pathogen infection reduced the suppression of nitrate reductase (NR) activity, reducing ammonium nitrogen (NH<sub>4</sub><sup>+</sup>-N) and nitrate nitrogen (NO<sub>3</sub><sup>-</sup>-N) assimilation, which reduces glutamine synthetase (GS), glutamate synthetase (GOGAT) activity and polynucleotide synthesis and promotes RNA degradation, resulting in a decrease in ribosome levels and protein synthesis. Cysteine was found to be associated with the symptoms of PBD, while alanine, lysine, proline, and glutamic acid were found to be involved in protective and regulatory mechanisms as well. Additionally, glutamate played an important role in sugarcane defense against pathogens through the biosynthesis of proline and polyamines. Cyanamide, glutamate, proline, tyrosine, and arachidonic acid metabolism actively participate in resistance and response to stress. C5XPZ6 and C5XCA6 were considered to be critical proteins and key effectors according to this study. In conclusion, we have identified potential proteins and pathways involved in sugarcane resistance to F. verticillioides, revealing new findings that may be useful in the design of future diagnostics or sugarcane protection strategies and providing new insights into the molecular mechanisms of sugarcane-pathogen interactions.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier GmbH. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Zeping</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture. Nanning, 530007, China; College of Agriculture, State Key Laboratory of Conservation and Utilization of Subtropical Agrobioresources, Guangxi University, Nanning, Guangxi, 530004, China. Electronic address: yaheng830619@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Qian</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Flowers Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, 530007, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shuai</LastName><ForeName>Liang</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>College of Food and Biological Engineering/Institute of Food Research, Hezhou University, Hezhou, 542899, Guangxi, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Htun</LastName><ForeName>Reemon</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Mandalay Technological University, Mandalay, Myanmar.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Malviya</LastName><ForeName>Mukesh Kumar</ForeName><Initials>MK</Initials><AffiliationInfo><Affiliation>Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture. Nanning, 530007, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yijie</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture. Nanning, 530007, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Qiang</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture. Nanning, 530007, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Gemin</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture. Nanning, 530007, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Muqing</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>College of Agriculture, State Key Laboratory of Conservation and Utilization of Subtropical Agrobioresources, Guangxi University, Nanning, Guangxi, 530004, China. Electronic address: 745784546@qq.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Fengjue</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>College of Agriculture, State Key Laboratory of Conservation and Utilization of Subtropical Agrobioresources, Guangxi University, Nanning, Guangxi, 530004, China. Electronic address: 2096799690@qq.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>06</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Plant Physiol</MedlineTA><NlmUniqueID>9882059</NlmUniqueID><ISSNLinking>0176-1617</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Fusarium verticillioides</Keyword><Keyword MajorTopicYN="N">Nitrogen metabolism</Keyword><Keyword MajorTopicYN="N">Pokkah boeng disease</Keyword><Keyword MajorTopicYN="N">Proteomic</Keyword><Keyword MajorTopicYN="N">Sugarcane</Keyword></KeywordList><CoiStatement>Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>09</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>05</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>05</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>6</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32593920</ArticleId><ArticleId IdType="pii">S0176-1617(20)30097-3</ArticleId><ArticleId IdType="doi">10.1016/j.jplph.2020.153207</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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