The UTP-glucose-1-phosphate uridylyltransferase of Brucella melitensis inhibits the activation of NF-κB via regulating the bacterial type IV secretion system.
Identifieur interne : 000137 ( Main/Corpus ); précédent : 000136; suivant : 000138The UTP-glucose-1-phosphate uridylyltransferase of Brucella melitensis inhibits the activation of NF-κB via regulating the bacterial type IV secretion system.
Auteurs : Yucheng Zhou ; Zhaoyang Bu ; Jing Qian ; Yuening Chen ; Lianjiang Qiao ; Sen Yang ; Shipeng Chen ; Xinglong Wang ; Linzhu Ren ; Yanling YangSource :
- International journal of biological macromolecules [ 1879-0003 ] ; 2020.
Abstract
UDP-glucose pyrophosphorylase (UGPase) is an important pyrophosphatase that reversibly catalyzes the synthesis of UDP-glucose during glucose metabolism. We previously found that the deletion of UGPase may affect structure, growth, the virulence of Brucella, and the activation of cellular NF-κB. However, the exact mechanism of activation of NF-κB regulated by Brucella UGPase is still unclear. Here, we found for the first time that UGPase can regulate the expression of virB proteins (virB3, virB4, virB5, virB6, virB8, virB9, virB10, and virB11) of type IV secretion system (T4SS) as well as effectors (vceC, btpA, btpB, ricA, bspB, bspC, and bspF) of Brucella by promoting the expression of ribosomal S12 protein (rpsL), BMEI1825, and quinone of 2,4,5-trihydroxyphenylalanine (topA) proteins, and further inhibits the activation of cellular NF-κB and affects the virulence of Brucella. Our findings provide new insights into the mechanism used by Brucella to escape the immune recognition, which is expected to be of great value in the designing of Brucella vaccines and the screening of drug targets.
DOI: 10.1016/j.ijbiomac.2020.08.134
PubMed: 32827613
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The UTP-glucose-1-phosphate uridylyltransferase of Brucella melitensis inhibits the activation of NF-κB via regulating the bacterial type IV secretion system.</title><author><name sortKey="Zhou, Yucheng" sort="Zhou, Yucheng" uniqKey="Zhou Y" first="Yucheng" last="Zhou">Yucheng Zhou</name><affiliation><nlm:affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Bu, Zhaoyang" sort="Bu, Zhaoyang" uniqKey="Bu Z" first="Zhaoyang" last="Bu">Zhaoyang Bu</name><affiliation><nlm:affiliation>Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Qian, Jing" sort="Qian, Jing" uniqKey="Qian J" first="Jing" last="Qian">Jing Qian</name><affiliation><nlm:affiliation>Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Yuening" sort="Chen, Yuening" uniqKey="Chen Y" first="Yuening" last="Chen">Yuening Chen</name><affiliation><nlm:affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Qiao, Lianjiang" sort="Qiao, Lianjiang" uniqKey="Qiao L" first="Lianjiang" last="Qiao">Lianjiang Qiao</name><affiliation><nlm:affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Sen" sort="Yang, Sen" uniqKey="Yang S" first="Sen" last="Yang">Sen Yang</name><affiliation><nlm:affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Shipeng" sort="Chen, Shipeng" uniqKey="Chen S" first="Shipeng" last="Chen">Shipeng Chen</name><affiliation><nlm:affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Xinglong" sort="Wang, Xinglong" uniqKey="Wang X" first="Xinglong" last="Wang">Xinglong Wang</name><affiliation><nlm:affiliation>Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ren, Linzhu" sort="Ren, Linzhu" uniqKey="Ren L" first="Linzhu" last="Ren">Linzhu Ren</name><affiliation><nlm:affiliation>Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. Electronic address: renlz@jlu.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Yanling" sort="Yang, Yanling" uniqKey="Yang Y" first="Yanling" last="Yang">Yanling Yang</name><affiliation><nlm:affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China; Jilin Academy of Agricultural Sciences, Changchun 130033, China. Electronic address: m18043213639@163.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32827613</idno><idno type="pmid">32827613</idno><idno type="doi">10.1016/j.ijbiomac.2020.08.134</idno><idno type="wicri:Area/Main/Corpus">000137</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000137</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The UTP-glucose-1-phosphate uridylyltransferase of Brucella melitensis inhibits the activation of NF-κB via regulating the bacterial type IV secretion system.</title><author><name sortKey="Zhou, Yucheng" sort="Zhou, Yucheng" uniqKey="Zhou Y" first="Yucheng" last="Zhou">Yucheng Zhou</name><affiliation><nlm:affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Bu, Zhaoyang" sort="Bu, Zhaoyang" uniqKey="Bu Z" first="Zhaoyang" last="Bu">Zhaoyang Bu</name><affiliation><nlm:affiliation>Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Qian, Jing" sort="Qian, Jing" uniqKey="Qian J" first="Jing" last="Qian">Jing Qian</name><affiliation><nlm:affiliation>Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Yuening" sort="Chen, Yuening" uniqKey="Chen Y" first="Yuening" last="Chen">Yuening Chen</name><affiliation><nlm:affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Qiao, Lianjiang" sort="Qiao, Lianjiang" uniqKey="Qiao L" first="Lianjiang" last="Qiao">Lianjiang Qiao</name><affiliation><nlm:affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Sen" sort="Yang, Sen" uniqKey="Yang S" first="Sen" last="Yang">Sen Yang</name><affiliation><nlm:affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Shipeng" sort="Chen, Shipeng" uniqKey="Chen S" first="Shipeng" last="Chen">Shipeng Chen</name><affiliation><nlm:affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Xinglong" sort="Wang, Xinglong" uniqKey="Wang X" first="Xinglong" last="Wang">Xinglong Wang</name><affiliation><nlm:affiliation>Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130112, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ren, Linzhu" sort="Ren, Linzhu" uniqKey="Ren L" first="Linzhu" last="Ren">Linzhu Ren</name><affiliation><nlm:affiliation>Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. Electronic address: renlz@jlu.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Yanling" sort="Yang, Yanling" uniqKey="Yang Y" first="Yanling" last="Yang">Yanling Yang</name><affiliation><nlm:affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China; Jilin Academy of Agricultural Sciences, Changchun 130033, China. Electronic address: m18043213639@163.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">International journal of biological macromolecules</title><idno type="eISSN">1879-0003</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">UDP-glucose pyrophosphorylase (UGPase) is an important pyrophosphatase that reversibly catalyzes the synthesis of UDP-glucose during glucose metabolism. We previously found that the deletion of UGPase may affect structure, growth, the virulence of Brucella, and the activation of cellular NF-κB. However, the exact mechanism of activation of NF-κB regulated by Brucella UGPase is still unclear. Here, we found for the first time that UGPase can regulate the expression of virB proteins (virB3, virB4, virB5, virB6, virB8, virB9, virB10, and virB11) of type IV secretion system (T4SS) as well as effectors (vceC, btpA, btpB, ricA, bspB, bspC, and bspF) of Brucella by promoting the expression of ribosomal S12 protein (rpsL), BMEI1825, and quinone of 2,4,5-trihydroxyphenylalanine (topA) proteins, and further inhibits the activation of cellular NF-κB and affects the virulence of Brucella. Our findings provide new insights into the mechanism used by Brucella to escape the immune recognition, which is expected to be of great value in the designing of Brucella vaccines and the screening of drug targets.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32827613</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0003</ISSN><JournalIssue CitedMedium="Internet"><Volume>164</Volume><PubDate><Year>2020</Year><Month>Dec</Month><Day>01</Day></PubDate></JournalIssue><Title>International journal of biological macromolecules</Title><ISOAbbreviation>Int J Biol Macromol</ISOAbbreviation></Journal><ArticleTitle>The UTP-glucose-1-phosphate uridylyltransferase of Brucella melitensis inhibits the activation of NF-κB via regulating the bacterial type IV secretion system.</ArticleTitle><Pagination><MedlinePgn>3098-3104</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0141-8130(20)34225-2</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ijbiomac.2020.08.134</ELocationID><Abstract><AbstractText>UDP-glucose pyrophosphorylase (UGPase) is an important pyrophosphatase that reversibly catalyzes the synthesis of UDP-glucose during glucose metabolism. We previously found that the deletion of UGPase may affect structure, growth, the virulence of Brucella, and the activation of cellular NF-κB. However, the exact mechanism of activation of NF-κB regulated by Brucella UGPase is still unclear. Here, we found for the first time that UGPase can regulate the expression of virB proteins (virB3, virB4, virB5, virB6, virB8, virB9, virB10, and virB11) of type IV secretion system (T4SS) as well as effectors (vceC, btpA, btpB, ricA, bspB, bspC, and bspF) of Brucella by promoting the expression of ribosomal S12 protein (rpsL), BMEI1825, and quinone of 2,4,5-trihydroxyphenylalanine (topA) proteins, and further inhibits the activation of cellular NF-κB and affects the virulence of Brucella. Our findings provide new insights into the mechanism used by Brucella to escape the immune recognition, which is expected to be of great value in the designing of Brucella vaccines and the screening of drug targets.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Yucheng</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bu</LastName><ForeName>Zhaoyang</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130112, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qian</LastName><ForeName>Jing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yuening</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qiao</LastName><ForeName>Lianjiang</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Sen</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Shipeng</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xinglong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130112, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Linzhu</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. Electronic address: renlz@jlu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yanling</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China; Jilin Academy of Agricultural Sciences, Changchun 130033, China. Electronic address: m18043213639@163.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Int J Biol Macromol</MedlineTA><NlmUniqueID>7909578</NlmUniqueID><ISSNLinking>0141-8130</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Brucella melitensis</Keyword><Keyword MajorTopicYN="N">Nuclear factor-kappa enhancer-binding protein (NF-κB)</Keyword><Keyword MajorTopicYN="N">Type IV secretion system (T4SS)</Keyword><Keyword MajorTopicYN="N">UTP-glucose-1-phosphate uridylyltransferase (UGPase)</Keyword></KeywordList><CoiStatement>Declaration of competing interest The authors declare no competing interests. All authors have approved the final version of the manuscript.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>07</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>08</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>08</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32827613</ArticleId><ArticleId IdType="pii">S0141-8130(20)34225-2</ArticleId><ArticleId IdType="doi">10.1016/j.ijbiomac.2020.08.134</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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