Phosphorylation of the Pseudomonas Effector AvrPtoB by Arabidopsis SnRK2.8 Is Required for Bacterial Virulence.
Identifieur interne : 000116 ( Main/Corpus ); précédent : 000115; suivant : 000117Phosphorylation of the Pseudomonas Effector AvrPtoB by Arabidopsis SnRK2.8 Is Required for Bacterial Virulence.
Auteurs : Lei Lei ; Danielle M. Stevens ; Gitta CoakerSource :
- Molecular plant [ 1752-9867 ] ; 2020.
Abstract
A critical component controlling bacterial virulence is the delivery of pathogen effectors into plant cells during infection. Effectors alter host metabolism and immunity for the benefit of pathogens. Multiple effectors are phosphorylated by host kinases, and this posttranslational modification is important for their activity. We sought to identify host kinases involved in effector phosphorylation. Multiple phosphorylated effector residues matched the proposed consensus motif for the plant calcium-dependent protein kinase (CDPK) and Snf1-related kinase (SnRK) superfamily. The conserved Pseudomonas effector AvrPtoB acts as an E3 ubiquitin ligase and promotes bacterial virulence. In this study, we identified a member of the Arabidopsis SnRK family, SnRK2.8, which interacts with AvrPtoB in yeast and in planta. We showed that SnRK2.8 was required for AvrPtoB virulence functions, including facilitating bacterial colonization, suppression of callose deposition, and targeting the plant defense regulator NPR1 and analyses receptor FLS2. Mass spectrometry analysis revealed that AvrPtoB phosphorylation occurs at multiple serine residues in planta, with S258 phosphorylation significantly reduced in the snrk2.8 knockout. AvrPtoB phospho-null mutants exhibited compromised virulence functions and were unable to suppress NPR1 accumulation, FLS2 accumulation, or inhibit FLS2-BAK1 complex formation upon flagellin perception. Taken together, these data identify a conserved plant kinase utilized by a pathogen effector to promote disease.
DOI: 10.1016/j.molp.2020.08.018
PubMed: 32889173
Links to Exploration step
pubmed:32889173Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Phosphorylation of the Pseudomonas Effector AvrPtoB by Arabidopsis SnRK2.8 Is Required for Bacterial Virulence.</title>
<author><name sortKey="Lei, Lei" sort="Lei, Lei" uniqKey="Lei L" first="Lei" last="Lei">Lei Lei</name>
<affiliation><nlm:affiliation>Department of Plant Pathology, University of California, Davis, Davis, CA, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Stevens, Danielle M" sort="Stevens, Danielle M" uniqKey="Stevens D" first="Danielle M" last="Stevens">Danielle M. Stevens</name>
<affiliation><nlm:affiliation>Department of Plant Pathology, University of California, Davis, Davis, CA, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Coaker, Gitta" sort="Coaker, Gitta" uniqKey="Coaker G" first="Gitta" last="Coaker">Gitta Coaker</name>
<affiliation><nlm:affiliation>Department of Plant Pathology, University of California, Davis, Davis, CA, USA. Electronic address: glcoaker@ucdavis.edu.</nlm:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2020">2020</date>
<idno type="RBID">pubmed:32889173</idno>
<idno type="pmid">32889173</idno>
<idno type="doi">10.1016/j.molp.2020.08.018</idno>
<idno type="wicri:Area/Main/Corpus">000116</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000116</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Phosphorylation of the Pseudomonas Effector AvrPtoB by Arabidopsis SnRK2.8 Is Required for Bacterial Virulence.</title>
<author><name sortKey="Lei, Lei" sort="Lei, Lei" uniqKey="Lei L" first="Lei" last="Lei">Lei Lei</name>
<affiliation><nlm:affiliation>Department of Plant Pathology, University of California, Davis, Davis, CA, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Stevens, Danielle M" sort="Stevens, Danielle M" uniqKey="Stevens D" first="Danielle M" last="Stevens">Danielle M. Stevens</name>
<affiliation><nlm:affiliation>Department of Plant Pathology, University of California, Davis, Davis, CA, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Coaker, Gitta" sort="Coaker, Gitta" uniqKey="Coaker G" first="Gitta" last="Coaker">Gitta Coaker</name>
<affiliation><nlm:affiliation>Department of Plant Pathology, University of California, Davis, Davis, CA, USA. Electronic address: glcoaker@ucdavis.edu.</nlm:affiliation>
</affiliation>
</author>
</analytic>
<series><title level="j">Molecular plant</title>
<idno type="eISSN">1752-9867</idno>
<imprint><date when="2020" type="published">2020</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A critical component controlling bacterial virulence is the delivery of pathogen effectors into plant cells during infection. Effectors alter host metabolism and immunity for the benefit of pathogens. Multiple effectors are phosphorylated by host kinases, and this posttranslational modification is important for their activity. We sought to identify host kinases involved in effector phosphorylation. Multiple phosphorylated effector residues matched the proposed consensus motif for the plant calcium-dependent protein kinase (CDPK) and Snf1-related kinase (SnRK) superfamily. The conserved Pseudomonas effector AvrPtoB acts as an E3 ubiquitin ligase and promotes bacterial virulence. In this study, we identified a member of the Arabidopsis SnRK family, SnRK2.8, which interacts with AvrPtoB in yeast and in planta. We showed that SnRK2.8 was required for AvrPtoB virulence functions, including facilitating bacterial colonization, suppression of callose deposition, and targeting the plant defense regulator NPR1 and analyses receptor FLS2. Mass spectrometry analysis revealed that AvrPtoB phosphorylation occurs at multiple serine residues in planta, with S258 phosphorylation significantly reduced in the snrk2.8 knockout. AvrPtoB phospho-null mutants exhibited compromised virulence functions and were unable to suppress NPR1 accumulation, FLS2 accumulation, or inhibit FLS2-BAK1 complex formation upon flagellin perception. Taken together, these data identify a conserved plant kinase utilized by a pathogen effector to promote disease.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">32889173</PMID>
<DateRevised><Year>2020</Year>
<Month>10</Month>
<Day>07</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1752-9867</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>13</Volume>
<Issue>10</Issue>
<PubDate><Year>2020</Year>
<Month>Oct</Month>
<Day>05</Day>
</PubDate>
</JournalIssue>
<Title>Molecular plant</Title>
<ISOAbbreviation>Mol Plant</ISOAbbreviation>
</Journal>
<ArticleTitle>Phosphorylation of the Pseudomonas Effector AvrPtoB by Arabidopsis SnRK2.8 Is Required for Bacterial Virulence.</ArticleTitle>
<Pagination><MedlinePgn>1513-1522</MedlinePgn>
</Pagination>
<ELocationID EIdType="pii" ValidYN="Y">S1674-2052(20)30295-1</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.molp.2020.08.018</ELocationID>
<Abstract><AbstractText>A critical component controlling bacterial virulence is the delivery of pathogen effectors into plant cells during infection. Effectors alter host metabolism and immunity for the benefit of pathogens. Multiple effectors are phosphorylated by host kinases, and this posttranslational modification is important for their activity. We sought to identify host kinases involved in effector phosphorylation. Multiple phosphorylated effector residues matched the proposed consensus motif for the plant calcium-dependent protein kinase (CDPK) and Snf1-related kinase (SnRK) superfamily. The conserved Pseudomonas effector AvrPtoB acts as an E3 ubiquitin ligase and promotes bacterial virulence. In this study, we identified a member of the Arabidopsis SnRK family, SnRK2.8, which interacts with AvrPtoB in yeast and in planta. We showed that SnRK2.8 was required for AvrPtoB virulence functions, including facilitating bacterial colonization, suppression of callose deposition, and targeting the plant defense regulator NPR1 and analyses receptor FLS2. Mass spectrometry analysis revealed that AvrPtoB phosphorylation occurs at multiple serine residues in planta, with S258 phosphorylation significantly reduced in the snrk2.8 knockout. AvrPtoB phospho-null mutants exhibited compromised virulence functions and were unable to suppress NPR1 accumulation, FLS2 accumulation, or inhibit FLS2-BAK1 complex formation upon flagellin perception. Taken together, these data identify a conserved plant kinase utilized by a pathogen effector to promote disease.</AbstractText>
<CopyrightInformation>Copyright © 2020 The Author. Published by Elsevier Inc. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lei</LastName>
<ForeName>Lei</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Department of Plant Pathology, University of California, Davis, Davis, CA, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Stevens</LastName>
<ForeName>Danielle M</ForeName>
<Initials>DM</Initials>
<AffiliationInfo><Affiliation>Department of Plant Pathology, University of California, Davis, Davis, CA, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Coaker</LastName>
<ForeName>Gitta</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Department of Plant Pathology, University of California, Davis, Davis, CA, USA. Electronic address: glcoaker@ucdavis.edu.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2020</Year>
<Month>09</Month>
<Day>02</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Mol Plant</MedlineTA>
<NlmUniqueID>101465514</NlmUniqueID>
<ISSNLinking>1674-2052</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arabidopsis</Keyword>
<Keyword MajorTopicYN="N">AvrPtoB</Keyword>
<Keyword MajorTopicYN="N">Pseudomonas syringae</Keyword>
<Keyword MajorTopicYN="N">SnRK2.8</Keyword>
<Keyword MajorTopicYN="N">effector</Keyword>
<Keyword MajorTopicYN="N">kinase</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year>
<Month>03</Month>
<Day>21</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2020</Year>
<Month>08</Month>
<Day>20</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2020</Year>
<Month>08</Month>
<Day>30</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2020</Year>
<Month>9</Month>
<Day>6</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>9</Month>
<Day>6</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2020</Year>
<Month>9</Month>
<Day>5</Day>
<Hour>12</Hour>
<Minute>21</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">32889173</ArticleId>
<ArticleId IdType="pii">S1674-2052(20)30295-1</ArticleId>
<ArticleId IdType="doi">10.1016/j.molp.2020.08.018</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PlantPathoEffV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000116 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000116 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= PlantPathoEffV1 |flux= Main |étape= Corpus |type= RBID |clé= pubmed:32889173 |texte= Phosphorylation of the Pseudomonas Effector AvrPtoB by Arabidopsis SnRK2.8 Is Required for Bacterial Virulence. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:32889173" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a PlantPathoEffV1
This area was generated with Dilib version V0.6.38. |