Transcriptome analysis of the grape-Elsinoë ampelina pathosystem reveals pathogenesis of E. ampelina associated with novel effectors and pathogenicity-related genes and defensive strategies of grapevine.
Identifieur interne : 000084 ( Main/Corpus ); précédent : 000083; suivant : 000085Transcriptome analysis of the grape-Elsinoë ampelina pathosystem reveals pathogenesis of E. ampelina associated with novel effectors and pathogenicity-related genes and defensive strategies of grapevine.
Auteurs : Zhi Li ; Ya Wang ; Yan Chun Fan ; Bilal Ahmad ; Xianhang Wang ; Songlin Zhang ; Yanxun Zhu ; Lin Lin Gao ; Ping Ping Chang ; Xiping WangSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2020.
Abstract
Elsinoë ampelina is an ascomycetous fungus that causes grape anthracnose, a potentially devastating disease worldwide. In this study, a dual RNA-seq analysis was used to simultaneously monitor the fungal genes related to pathogenesis and grape genes related to defence during the interaction at 2, 3, 4, and 5 days post inoculation (dpi). Consistent with their potential roles in pathogenicity, genes for carbohydrate-active enzymes, secondary metabolites synthesis, pathogen-host interaction and those encoding secreted proteins are upregulated during infection. Based on Agrobacterium tumefaciens-mediated transient assays in Nicotiana benthamiana, we further showed that eight and nine candidate effectors suppressed BAX- and INF1-mediated programmed cell death, respectively. The host response was characterized by the induction multiple defense systems against E. ampelina, including synthesis of phenylpropanoids, stilbenes, and terpenoids biosynthesis, cell wall modifications, regulation by phytohormones, and expression of defense related genes. Together, these findings offer new insights into the molecular mechanisms underlying the grape-E. ampelina interaction.
DOI: 10.1094/MPMI-08-20-0227-R
PubMed: 33006532
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transcriptome analysis of the grape-Elsinoë ampelina pathosystem reveals pathogenesis of E. ampelina associated with novel effectors and pathogenicity-related genes and defensive strategies of grapevine.</title><author><name sortKey="Li, Zhi" sort="Li, Zhi" uniqKey="Li Z" first="Zhi" last="Li">Zhi Li</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, College of Horticulture, Taicheng Road No. 3, Yangling, China, 712100; lizhi@nwsuaf.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Ya" sort="Wang, Ya" uniqKey="Wang Y" first="Ya" last="Wang">Ya Wang</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; wangya526173087@126.com.</nlm:affiliation></affiliation></author><author><name sortKey="Fan, Yan Chun" sort="Fan, Yan Chun" uniqKey="Fan Y" first="Yan Chun" last="Fan">Yan Chun Fan</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, College of Forestry, Yangling, Shaanxi, China; fanyanchun666@163.com.</nlm:affiliation></affiliation></author><author><name sortKey="Ahmad, Bilal" sort="Ahmad, Bilal" uniqKey="Ahmad B" first="Bilal" last="Ahmad">Bilal Ahmad</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; bajwa1999@nwafu.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Xianhang" sort="Wang, Xianhang" uniqKey="Wang X" first="Xianhang" last="Wang">Xianhang Wang</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; wangxianhang@nwsuaf.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Songlin" sort="Zhang, Songlin" uniqKey="Zhang S" first="Songlin" last="Zhang">Songlin Zhang</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; 18821636061@163.com.</nlm:affiliation></affiliation></author><author><name sortKey="Zhu, Yanxun" sort="Zhu, Yanxun" uniqKey="Zhu Y" first="Yanxun" last="Zhu">Yanxun Zhu</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; 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lizhi@nwsuaf.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Ya" sort="Wang, Ya" uniqKey="Wang Y" first="Ya" last="Wang">Ya Wang</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; wangya526173087@126.com.</nlm:affiliation></affiliation></author><author><name sortKey="Fan, Yan Chun" sort="Fan, Yan Chun" uniqKey="Fan Y" first="Yan Chun" last="Fan">Yan Chun Fan</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, College of Forestry, Yangling, Shaanxi, China; fanyanchun666@163.com.</nlm:affiliation></affiliation></author><author><name sortKey="Ahmad, Bilal" sort="Ahmad, Bilal" uniqKey="Ahmad B" first="Bilal" last="Ahmad">Bilal Ahmad</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; bajwa1999@nwafu.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Xianhang" sort="Wang, Xianhang" uniqKey="Wang X" first="Xianhang" last="Wang">Xianhang Wang</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; wangxianhang@nwsuaf.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Songlin" sort="Zhang, Songlin" uniqKey="Zhang S" first="Songlin" last="Zhang">Songlin Zhang</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; 18821636061@163.com.</nlm:affiliation></affiliation></author><author><name sortKey="Zhu, Yanxun" sort="Zhu, Yanxun" uniqKey="Zhu Y" first="Yanxun" last="Zhu">Yanxun Zhu</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; yanxun.zhu@nwsuaf.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Gao, Lin Lin" sort="Gao, Lin Lin" uniqKey="Gao L" first="Lin Lin" last="Gao">Lin Lin Gao</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, College of Horticulture, Yangling, Shaanxi, China; gaolinlin@nwafu.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Chang, Ping Ping" sort="Chang, Ping Ping" uniqKey="Chang P" first="Ping Ping" last="Chang">Ping Ping Chang</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, College of Horticulture, Yangling, Shaanxi, China; changpingping@nwafu.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Xiping" sort="Wang, Xiping" uniqKey="Wang X" first="Xiping" last="Wang">Xiping Wang</name><affiliation><nlm:affiliation>Northwest A&F University, 12469, No.3, Taicheng Road, Yangling, China, 712100; wangxiping@nwsuaf.edu.cn.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Elsinoë ampelina is an ascomycetous fungus that causes grape anthracnose, a potentially devastating disease worldwide. In this study, a dual RNA-seq analysis was used to simultaneously monitor the fungal genes related to pathogenesis and grape genes related to defence during the interaction at 2, 3, 4, and 5 days post inoculation (dpi). Consistent with their potential roles in pathogenicity, genes for carbohydrate-active enzymes, secondary metabolites synthesis, pathogen-host interaction and those encoding secreted proteins are upregulated during infection. Based on Agrobacterium tumefaciens-mediated transient assays in Nicotiana benthamiana, we further showed that eight and nine candidate effectors suppressed BAX- and INF1-mediated programmed cell death, respectively. The host response was characterized by the induction multiple defense systems against E. ampelina, including synthesis of phenylpropanoids, stilbenes, and terpenoids biosynthesis, cell wall modifications, regulation by phytohormones, and expression of defense related genes. Together, these findings offer new insights into the molecular mechanisms underlying the grape-E. ampelina interaction.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33006532</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><PubDate><Year>2020</Year><Month>Oct</Month><Day>02</Day></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Transcriptome analysis of the grape-Elsinoë ampelina pathosystem reveals pathogenesis of E. ampelina associated with novel effectors and pathogenicity-related genes and defensive strategies of grapevine.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-08-20-0227-R</ELocationID><Abstract><AbstractText>Elsinoë ampelina is an ascomycetous fungus that causes grape anthracnose, a potentially devastating disease worldwide. In this study, a dual RNA-seq analysis was used to simultaneously monitor the fungal genes related to pathogenesis and grape genes related to defence during the interaction at 2, 3, 4, and 5 days post inoculation (dpi). Consistent with their potential roles in pathogenicity, genes for carbohydrate-active enzymes, secondary metabolites synthesis, pathogen-host interaction and those encoding secreted proteins are upregulated during infection. Based on Agrobacterium tumefaciens-mediated transient assays in Nicotiana benthamiana, we further showed that eight and nine candidate effectors suppressed BAX- and INF1-mediated programmed cell death, respectively. The host response was characterized by the induction multiple defense systems against E. ampelina, including synthesis of phenylpropanoids, stilbenes, and terpenoids biosynthesis, cell wall modifications, regulation by phytohormones, and expression of defense related genes. Together, these findings offer new insights into the molecular mechanisms underlying the grape-E. ampelina interaction.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Zhi</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Northwest A&F University, 12469, College of Horticulture, Taicheng Road No. 3, Yangling, China, 712100; lizhi@nwsuaf.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Ya</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; wangya526173087@126.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fan</LastName><ForeName>Yan Chun</ForeName><Initials>YC</Initials><AffiliationInfo><Affiliation>Northwest A&F University, 12469, College of Forestry, Yangling, Shaanxi, China; fanyanchun666@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ahmad</LastName><ForeName>Bilal</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; bajwa1999@nwafu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xianhang</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; wangxianhang@nwsuaf.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Songlin</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; 18821636061@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Yanxun</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Northwest A&F University, 12469, Yangling, Shaanxi, China; yanxun.zhu@nwsuaf.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Lin Lin</ForeName><Initials>LL</Initials><AffiliationInfo><Affiliation>Northwest A&F University, 12469, College of Horticulture, Yangling, Shaanxi, China; gaolinlin@nwafu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Ping Ping</ForeName><Initials>PP</Initials><AffiliationInfo><Affiliation>Northwest A&F University, 12469, College of Horticulture, Yangling, Shaanxi, China; changpingping@nwafu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xiping</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Northwest A&F University, 12469, No.3, Taicheng Road, Yangling, China, 712100; wangxiping@nwsuaf.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>2</Day><Hour>12</Hour><Minute>8</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33006532</ArticleId><ArticleId IdType="doi">10.1094/MPMI-08-20-0227-R</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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