RNA sequencing analyses of gene expressions in a canine macrophages cell line DH82 infected with canine distemper virus.
Identifieur interne : 000237 ( Main/Corpus ); précédent : 000236; suivant : 000238RNA sequencing analyses of gene expressions in a canine macrophages cell line DH82 infected with canine distemper virus.
Auteurs : Xuexing Zheng ; Yelei Zhu ; Zhongxin Zhao ; Lina Yan ; Tong Xu ; Xianwei Wang ; Hongbin He ; Xianzhu Xia ; Wenwen Zheng ; Xianghong XueSource :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [ 1567-7257 ] ; 2020.
Abstract
Virulent morbillivirus infections, including Meals Virus (MeV) and Canine Distemper Virus (CDV), caused severe immune suppression and leukopenia, while attenuated vaccine strains developed protective host immune responses. However, the detailed molecular foundations of host antiviral responses were poorly characterized. In order to better understand the interactions between attenuated vaccine and host antiviral responses, the global gene expression changes in CDV-11-infected DH82 cells, a macrophage-derived cell line from canine, were investigated by transcriptomic analysis, and portions of results were confirmed with quantitative RT-PCR. The results exhibited that 372 genes significantly up-regulated (p < .01) and 119 genes were significantly down-regulated (p < .01) in CDV-infected macrophages DH82 at 48 h p.i.. The enriched functions of the significantly up-regulated (p < .01) genes were closely associated with interferon stimulated genes (ISGs), chemokine genes and pro-inflammatory factor genes. Gene ontology and pathway analysis of differentially expressed genes (DEGs) revealed that the most significantly involved pathways in CDV-infected DH82 cells were NF-κB and TNF signaling pathway, cytokine-cytokine receptor interaction, and pathogen associated molecular patterns (PAMPs), such as Toll-like, RIG-I-like and NOD-like receptor signalings. Thus, the findings indicated that pattern recognition receptors (PRRs) possibly mediated host innate and protective antiviral immune responses in CDV-11 infected DH82 cells.
DOI: 10.1016/j.meegid.2020.104206
PubMed: 31982604
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Electronic address: zhengxx2513@163.com.</nlm:affiliation></affiliation></author><author><name sortKey="Zhu, Yelei" sort="Zhu, Yelei" uniqKey="Zhu Y" first="Yelei" last="Zhu">Yelei Zhu</name><affiliation><nlm:affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China; Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhao, Zhongxin" sort="Zhao, Zhongxin" uniqKey="Zhao Z" first="Zhongxin" last="Zhao">Zhongxin Zhao</name><affiliation><nlm:affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yan, Lina" sort="Yan, Lina" uniqKey="Yan L" first="Lina" last="Yan">Lina Yan</name><affiliation><nlm:affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Tong" sort="Xu, Tong" uniqKey="Xu T" first="Tong" last="Xu">Tong Xu</name><affiliation><nlm:affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Xianwei" sort="Wang, Xianwei" uniqKey="Wang X" first="Xianwei" last="Wang">Xianwei Wang</name><affiliation><nlm:affiliation>College of Life Sciences, Shandong University, Qingdao 266237, China.</nlm:affiliation></affiliation></author><author><name sortKey="He, Hongbin" sort="He, Hongbin" uniqKey="He H" first="Hongbin" last="He">Hongbin He</name><affiliation><nlm:affiliation>College of Life Sciences, Shandong Normal University, Jinan 250014, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><affiliation><nlm:affiliation>Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zheng, Wenwen" sort="Zheng, Wenwen" uniqKey="Zheng W" first="Wenwen" last="Zheng">Wenwen Zheng</name><affiliation><nlm:affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xue, Xianghong" sort="Xue, Xianghong" uniqKey="Xue X" first="Xianghong" last="Xue">Xianghong Xue</name><affiliation><nlm:affiliation>Division of Infectious Diseases of Special Animal, Institute of Special Animal and Plant Sciences, The Chinese Academy of Agricultural Sciences, Changchun 130122, China. Electronic address: red99.smile@163.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31982604</idno><idno type="pmid">31982604</idno><idno type="doi">10.1016/j.meegid.2020.104206</idno><idno type="wicri:Area/Main/Corpus">000237</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000237</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">RNA sequencing analyses of gene expressions in a canine macrophages cell line DH82 infected with canine distemper virus.</title><author><name sortKey="Zheng, Xuexing" sort="Zheng, Xuexing" uniqKey="Zheng X" first="Xuexing" last="Zheng">Xuexing Zheng</name><affiliation><nlm:affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China. Electronic address: zhengxx2513@163.com.</nlm:affiliation></affiliation></author><author><name sortKey="Zhu, Yelei" sort="Zhu, Yelei" uniqKey="Zhu Y" first="Yelei" last="Zhu">Yelei Zhu</name><affiliation><nlm:affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China; Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhao, Zhongxin" sort="Zhao, Zhongxin" uniqKey="Zhao Z" first="Zhongxin" last="Zhao">Zhongxin Zhao</name><affiliation><nlm:affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yan, Lina" sort="Yan, Lina" uniqKey="Yan L" first="Lina" last="Yan">Lina Yan</name><affiliation><nlm:affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Tong" sort="Xu, Tong" uniqKey="Xu T" first="Tong" last="Xu">Tong Xu</name><affiliation><nlm:affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Xianwei" sort="Wang, Xianwei" uniqKey="Wang X" first="Xianwei" last="Wang">Xianwei Wang</name><affiliation><nlm:affiliation>College of Life Sciences, Shandong University, Qingdao 266237, China.</nlm:affiliation></affiliation></author><author><name sortKey="He, Hongbin" sort="He, Hongbin" uniqKey="He H" first="Hongbin" last="He">Hongbin He</name><affiliation><nlm:affiliation>College of Life Sciences, Shandong Normal University, Jinan 250014, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><affiliation><nlm:affiliation>Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zheng, Wenwen" sort="Zheng, Wenwen" uniqKey="Zheng W" first="Wenwen" last="Zheng">Wenwen Zheng</name><affiliation><nlm:affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xue, Xianghong" sort="Xue, Xianghong" uniqKey="Xue X" first="Xianghong" last="Xue">Xianghong Xue</name><affiliation><nlm:affiliation>Division of Infectious Diseases of Special Animal, Institute of Special Animal and Plant Sciences, The Chinese Academy of Agricultural Sciences, Changchun 130122, China. Electronic address: red99.smile@163.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases</title><idno type="eISSN">1567-7257</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Virulent morbillivirus infections, including Meals Virus (MeV) and Canine Distemper Virus (CDV), caused severe immune suppression and leukopenia, while attenuated vaccine strains developed protective host immune responses. However, the detailed molecular foundations of host antiviral responses were poorly characterized. In order to better understand the interactions between attenuated vaccine and host antiviral responses, the global gene expression changes in CDV-11-infected DH82 cells, a macrophage-derived cell line from canine, were investigated by transcriptomic analysis, and portions of results were confirmed with quantitative RT-PCR. The results exhibited that 372 genes significantly up-regulated (p < .01) and 119 genes were significantly down-regulated (p < .01) in CDV-infected macrophages DH82 at 48 h p.i.. The enriched functions of the significantly up-regulated (p < .01) genes were closely associated with interferon stimulated genes (ISGs), chemokine genes and pro-inflammatory factor genes. Gene ontology and pathway analysis of differentially expressed genes (DEGs) revealed that the most significantly involved pathways in CDV-infected DH82 cells were NF-κB and TNF signaling pathway, cytokine-cytokine receptor interaction, and pathogen associated molecular patterns (PAMPs), such as Toll-like, RIG-I-like and NOD-like receptor signalings. Thus, the findings indicated that pattern recognition receptors (PRRs) possibly mediated host innate and protective antiviral immune responses in CDV-11 infected DH82 cells.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31982604</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1567-7257</ISSN><JournalIssue CitedMedium="Internet"><Volume>80</Volume><PubDate><Year>2020</Year><Month>06</Month></PubDate></JournalIssue><Title>Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases</Title><ISOAbbreviation>Infect Genet Evol</ISOAbbreviation></Journal><ArticleTitle>RNA sequencing analyses of gene expressions in a canine macrophages cell line DH82 infected with canine distemper virus.</ArticleTitle><Pagination><MedlinePgn>104206</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1567-1348(20)30038-1</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.meegid.2020.104206</ELocationID><Abstract><AbstractText>Virulent morbillivirus infections, including Meals Virus (MeV) and Canine Distemper Virus (CDV), caused severe immune suppression and leukopenia, while attenuated vaccine strains developed protective host immune responses. However, the detailed molecular foundations of host antiviral responses were poorly characterized. In order to better understand the interactions between attenuated vaccine and host antiviral responses, the global gene expression changes in CDV-11-infected DH82 cells, a macrophage-derived cell line from canine, were investigated by transcriptomic analysis, and portions of results were confirmed with quantitative RT-PCR. The results exhibited that 372 genes significantly up-regulated (p < .01) and 119 genes were significantly down-regulated (p < .01) in CDV-infected macrophages DH82 at 48 h p.i.. The enriched functions of the significantly up-regulated (p < .01) genes were closely associated with interferon stimulated genes (ISGs), chemokine genes and pro-inflammatory factor genes. Gene ontology and pathway analysis of differentially expressed genes (DEGs) revealed that the most significantly involved pathways in CDV-infected DH82 cells were NF-κB and TNF signaling pathway, cytokine-cytokine receptor interaction, and pathogen associated molecular patterns (PAMPs), such as Toll-like, RIG-I-like and NOD-like receptor signalings. Thus, the findings indicated that pattern recognition receptors (PRRs) possibly mediated host innate and protective antiviral immune responses in CDV-11 infected DH82 cells.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Xuexing</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China. Electronic address: zhengxx2513@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Yelei</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China; Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Zhongxin</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Lina</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Tong</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xianwei</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Shandong University, Qingdao 266237, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Hongbin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Shandong Normal University, Jinan 250014, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xia</LastName><ForeName>Xianzhu</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Wenwen</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xue</LastName><ForeName>Xianghong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Division of Infectious Diseases of Special Animal, Institute of Special Animal and Plant Sciences, The Chinese Academy of Agricultural Sciences, Changchun 130122, China. Electronic address: red99.smile@163.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>01</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Infect Genet Evol</MedlineTA><NlmUniqueID>101084138</NlmUniqueID><ISSNLinking>1567-1348</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Antiviral responses</Keyword><Keyword MajorTopicYN="Y">Canine distemper virus</Keyword><Keyword MajorTopicYN="Y">Differentially expression genes</Keyword><Keyword MajorTopicYN="Y">Macrophages</Keyword><Keyword MajorTopicYN="Y">Transcriptomic</Keyword></KeywordList><CoiStatement>Declaration of Competing Interest No conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>10</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>01</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>01</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>1</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>1</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>1</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31982604</ArticleId><ArticleId IdType="pii">S1567-1348(20)30038-1</ArticleId><ArticleId IdType="doi">10.1016/j.meegid.2020.104206</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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