Identification of β tubulin IVb as a pattern recognition receptor with opsonic activity.
Identifieur interne : 000136 ( Main/Corpus ); précédent : 000135; suivant : 000137Identification of β tubulin IVb as a pattern recognition receptor with opsonic activity.
Auteurs : Huimin Duan ; Shuaiqi Yang ; Shousheng Ni ; Zengyu Ma ; Jianrui Yuan ; Shicui ZhangSource :
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [ 1532-0456 ] ; 2020.
Abstract
Previous studies have shown that tubulins play important role in immune responses of both plants and animals, but no experiments have been performed to study the mode of action of tubulins in immune defense. In addition, there is little convincing experimental evidence of functional commitment for specific tubulin isotypes in animals. In the present, we showed that expression of β-tubulin IVb gene was affected by both LPS and LTA, hinting its involvement in anti-infectious response. We also showed that recombinant zebrafish β-tubulin IVb not only interacted with LPS and LTA as well as Gram-negative and -positive bacteria but also agglutinated both Gram-negative and -positive bacteria in a Ca2+-dependent fashion. Interestingly, recombinant β-tubulin IVb could enhance the phagocytosis of bacteria by macrophages. Moreover, we demonstrated that β-tubulin IVb was present extracellularly in the serum of zebrafish and mouse. Collectively, these suggest that β-tubulin IVb may be physiologically involved in the systematic immunity of host via acting as a pattern recognition receptor and an opsonin. This also provides a new angle to understand the roles of β-tubulin IVb.
DOI: 10.1016/j.cbpc.2020.108781
PubMed: 32387479
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Electronic address: sczhang@ouc.edu.cn.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32387479</idno><idno type="pmid">32387479</idno><idno type="doi">10.1016/j.cbpc.2020.108781</idno><idno type="wicri:Area/Main/Corpus">000136</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000136</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of β tubulin IVb as a pattern recognition receptor with opsonic activity.</title><author><name sortKey="Duan, Huimin" sort="Duan, Huimin" uniqKey="Duan H" first="Huimin" last="Duan">Huimin Duan</name><affiliation><nlm:affiliation>Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Shuaiqi" sort="Yang, Shuaiqi" uniqKey="Yang S" first="Shuaiqi" last="Yang">Shuaiqi Yang</name><affiliation><nlm:affiliation>Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ni, Shousheng" sort="Ni, Shousheng" uniqKey="Ni S" first="Shousheng" last="Ni">Shousheng Ni</name><affiliation><nlm:affiliation>Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ma, Zengyu" sort="Ma, Zengyu" uniqKey="Ma Z" first="Zengyu" last="Ma">Zengyu Ma</name><affiliation><nlm:affiliation>Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yuan, Jianrui" sort="Yuan, Jianrui" uniqKey="Yuan J" first="Jianrui" last="Yuan">Jianrui Yuan</name><affiliation><nlm:affiliation>Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Shicui" sort="Zhang, Shicui" uniqKey="Zhang S" first="Shicui" last="Zhang">Shicui Zhang</name><affiliation><nlm:affiliation>Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266003, China. Electronic address: sczhang@ouc.edu.cn.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Comparative biochemistry and physiology. Toxicology & pharmacology : CBP</title><idno type="ISSN">1532-0456</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Previous studies have shown that tubulins play important role in immune responses of both plants and animals, but no experiments have been performed to study the mode of action of tubulins in immune defense. In addition, there is little convincing experimental evidence of functional commitment for specific tubulin isotypes in animals. In the present, we showed that expression of β-tubulin IVb gene was affected by both LPS and LTA, hinting its involvement in anti-infectious response. We also showed that recombinant zebrafish β-tubulin IVb not only interacted with LPS and LTA as well as Gram-negative and -positive bacteria but also agglutinated both Gram-negative and -positive bacteria in a Ca<sup>2+</sup>-dependent fashion. Interestingly, recombinant β-tubulin IVb could enhance the phagocytosis of bacteria by macrophages. Moreover, we demonstrated that β-tubulin IVb was present extracellularly in the serum of zebrafish and mouse. Collectively, these suggest that β-tubulin IVb may be physiologically involved in the systematic immunity of host via acting as a pattern recognition receptor and an opsonin. This also provides a new angle to understand the roles of β-tubulin IVb.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32387479</PMID><DateRevised><Year>2020</Year><Month>06</Month><Day>22</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1532-0456</ISSN><JournalIssue CitedMedium="Internet"><Volume>235</Volume><PubDate><Year>2020</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Comparative biochemistry and physiology. Toxicology & pharmacology : CBP</Title><ISOAbbreviation>Comp Biochem Physiol C Toxicol Pharmacol</ISOAbbreviation></Journal><ArticleTitle>Identification of β tubulin IVb as a pattern recognition receptor with opsonic activity.</ArticleTitle><Pagination><MedlinePgn>108781</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1532-0456(20)30081-8</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cbpc.2020.108781</ELocationID><Abstract><AbstractText>Previous studies have shown that tubulins play important role in immune responses of both plants and animals, but no experiments have been performed to study the mode of action of tubulins in immune defense. In addition, there is little convincing experimental evidence of functional commitment for specific tubulin isotypes in animals. In the present, we showed that expression of β-tubulin IVb gene was affected by both LPS and LTA, hinting its involvement in anti-infectious response. We also showed that recombinant zebrafish β-tubulin IVb not only interacted with LPS and LTA as well as Gram-negative and -positive bacteria but also agglutinated both Gram-negative and -positive bacteria in a Ca<sup>2+</sup>-dependent fashion. Interestingly, recombinant β-tubulin IVb could enhance the phagocytosis of bacteria by macrophages. Moreover, we demonstrated that β-tubulin IVb was present extracellularly in the serum of zebrafish and mouse. Collectively, these suggest that β-tubulin IVb may be physiologically involved in the systematic immunity of host via acting as a pattern recognition receptor and an opsonin. This also provides a new angle to understand the roles of β-tubulin IVb.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Duan</LastName><ForeName>Huimin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Shuaiqi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ni</LastName><ForeName>Shousheng</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Zengyu</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Jianrui</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Shicui</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266003, China. Electronic address: sczhang@ouc.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>05</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Comp Biochem Physiol C Toxicol Pharmacol</MedlineTA><NlmUniqueID>100959500</NlmUniqueID><ISSNLinking>1532-0456</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">LPS/LTA</Keyword><Keyword MajorTopicYN="N">Opsonin</Keyword><Keyword MajorTopicYN="N">Pattern recognition receptor</Keyword><Keyword MajorTopicYN="N">Tubulin</Keyword><Keyword MajorTopicYN="N">Zebrafish</Keyword></KeywordList><CoiStatement>Declaration of competing interest The authors declare no financial or commercial conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>03</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>04</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>04</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32387479</ArticleId><ArticleId IdType="pii">S1532-0456(20)30081-8</ArticleId><ArticleId IdType="doi">10.1016/j.cbpc.2020.108781</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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