Selection of Immunobiotic Ligilactobacillus salivarius Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies.
Identifieur interne : 000021 ( Main/Corpus ); précédent : 000020; suivant : 000022Selection of Immunobiotic Ligilactobacillus salivarius Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies.
Auteurs : Binghui Zhou ; Leonardo Albarracin ; Yuhki Indo ; Lorena Arce ; Yuki Masumizu ; Mikado Tomokiyo ; Md Aminul Islam ; Valeria Garcia-Castillo ; Wakako Ikeda-Ohtsubo ; Tomonori Nochi ; Hidetoshi Morita ; Hideki Takahashi ; Shoichiro Kurata ; Julio Villena ; Haruki KitazawaSource :
- Microorganisms [ 2076-2607 ] ; 2020.
Abstract
In this article, Ligilactobacillus salivarius FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine L. salivarius strains selected from subgroups that demonstrated different "immune" and "adhesion" phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that L. salivarius FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, L. salivarius FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several L. salivarius strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation.
DOI: 10.3390/microorganisms8111659
PubMed: 33114778
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Selection of Immunobiotic <i>Ligilactobacillus salivarius</i> Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies.</title><author><name sortKey="Zhou, Binghui" sort="Zhou, Binghui" uniqKey="Zhou B" first="Binghui" last="Zhou">Binghui Zhou</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Albarracin, Leonardo" sort="Albarracin, Leonardo" uniqKey="Albarracin L" first="Leonardo" last="Albarracin">Leonardo Albarracin</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Scientific Computing Laboratory, Computer Science Department, Faculty of Exact Sciences and Technology, National University of Tucuman, Tucuman 4000, Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Indo, Yuhki" sort="Indo, Yuhki" uniqKey="Indo Y" first="Yuhki" last="Indo">Yuhki Indo</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Arce, Lorena" sort="Arce, Lorena" uniqKey="Arce L" first="Lorena" last="Arce">Lorena Arce</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Infection Biology Laboratory, INSIBIO-CONICET, Faculty of Medicine, University of Tucuman, Tucuman 4000, Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Masumizu, Yuki" sort="Masumizu, Yuki" uniqKey="Masumizu Y" first="Yuki" last="Masumizu">Yuki Masumizu</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Tomokiyo, Mikado" sort="Tomokiyo, Mikado" uniqKey="Tomokiyo M" first="Mikado" last="Tomokiyo">Mikado Tomokiyo</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Islam, Md Aminul" sort="Islam, Md Aminul" uniqKey="Islam M" first="Md Aminul" last="Islam">Md Aminul Islam</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.</nlm:affiliation></affiliation></author><author><name sortKey="Garcia Castillo, Valeria" sort="Garcia Castillo, Valeria" uniqKey="Garcia Castillo V" first="Valeria" last="Garcia-Castillo">Valeria Garcia-Castillo</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Ikeda Ohtsubo, Wakako" sort="Ikeda Ohtsubo, Wakako" uniqKey="Ikeda Ohtsubo W" first="Wakako" last="Ikeda-Ohtsubo">Wakako Ikeda-Ohtsubo</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Nochi, Tomonori" sort="Nochi, Tomonori" uniqKey="Nochi T" first="Tomonori" last="Nochi">Tomonori Nochi</name><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Morita, Hidetoshi" sort="Morita, Hidetoshi" uniqKey="Morita H" first="Hidetoshi" last="Morita">Hidetoshi Morita</name><affiliation><nlm:affiliation>Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Takahashi, Hideki" sort="Takahashi, Hideki" uniqKey="Takahashi H" first="Hideki" last="Takahashi">Hideki Takahashi</name><affiliation><nlm:affiliation>Laboratory of Plant Pathology, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Plant Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Kurata, Shoichiro" sort="Kurata, Shoichiro" uniqKey="Kurata S" first="Shoichiro" last="Kurata">Shoichiro Kurata</name><affiliation><nlm:affiliation>Laboratory of Molecular Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Villena, Julio" sort="Villena, Julio" uniqKey="Villena J" first="Julio" last="Villena">Julio Villena</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Kitazawa, Haruki" sort="Kitazawa, Haruki" uniqKey="Kitazawa H" first="Haruki" last="Kitazawa">Haruki Kitazawa</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33114778</idno><idno type="pmid">33114778</idno><idno type="doi">10.3390/microorganisms8111659</idno><idno type="wicri:Area/Main/Corpus">000021</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000021</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Selection of Immunobiotic <i>Ligilactobacillus salivarius</i> Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies.</title><author><name sortKey="Zhou, Binghui" sort="Zhou, Binghui" uniqKey="Zhou B" first="Binghui" last="Zhou">Binghui Zhou</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Albarracin, Leonardo" sort="Albarracin, Leonardo" uniqKey="Albarracin L" first="Leonardo" last="Albarracin">Leonardo Albarracin</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Scientific Computing Laboratory, Computer Science Department, Faculty of Exact Sciences and Technology, National University of Tucuman, Tucuman 4000, Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Indo, Yuhki" sort="Indo, Yuhki" uniqKey="Indo Y" first="Yuhki" last="Indo">Yuhki Indo</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Arce, Lorena" sort="Arce, Lorena" uniqKey="Arce L" first="Lorena" last="Arce">Lorena Arce</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Infection Biology Laboratory, INSIBIO-CONICET, Faculty of Medicine, University of Tucuman, Tucuman 4000, Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Masumizu, Yuki" sort="Masumizu, Yuki" uniqKey="Masumizu Y" first="Yuki" last="Masumizu">Yuki Masumizu</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Tomokiyo, Mikado" sort="Tomokiyo, Mikado" uniqKey="Tomokiyo M" first="Mikado" last="Tomokiyo">Mikado Tomokiyo</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Islam, Md Aminul" sort="Islam, Md Aminul" uniqKey="Islam M" first="Md Aminul" last="Islam">Md Aminul Islam</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.</nlm:affiliation></affiliation></author><author><name sortKey="Garcia Castillo, Valeria" sort="Garcia Castillo, Valeria" uniqKey="Garcia Castillo V" first="Valeria" last="Garcia-Castillo">Valeria Garcia-Castillo</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Ikeda Ohtsubo, Wakako" sort="Ikeda Ohtsubo, Wakako" uniqKey="Ikeda Ohtsubo W" first="Wakako" last="Ikeda-Ohtsubo">Wakako Ikeda-Ohtsubo</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Nochi, Tomonori" sort="Nochi, Tomonori" uniqKey="Nochi T" first="Tomonori" last="Nochi">Tomonori Nochi</name><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Morita, Hidetoshi" sort="Morita, Hidetoshi" uniqKey="Morita H" first="Hidetoshi" last="Morita">Hidetoshi Morita</name><affiliation><nlm:affiliation>Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Takahashi, Hideki" sort="Takahashi, Hideki" uniqKey="Takahashi H" first="Hideki" last="Takahashi">Hideki Takahashi</name><affiliation><nlm:affiliation>Laboratory of Plant Pathology, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Plant Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Kurata, Shoichiro" sort="Kurata, Shoichiro" uniqKey="Kurata S" first="Shoichiro" last="Kurata">Shoichiro Kurata</name><affiliation><nlm:affiliation>Laboratory of Molecular Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Villena, Julio" sort="Villena, Julio" uniqKey="Villena J" first="Julio" last="Villena">Julio Villena</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Kitazawa, Haruki" sort="Kitazawa, Haruki" uniqKey="Kitazawa H" first="Haruki" last="Kitazawa">Haruki Kitazawa</name><affiliation><nlm:affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Microorganisms</title><idno type="ISSN">2076-2607</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this article, <i>Ligilactobacillus salivarius</i> FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine <i>L. salivarius</i> strains selected from subgroups that demonstrated different "immune" and "adhesion" phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that <i>L. salivarius</i> FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, <i>L. salivarius</i> FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several <i>L. salivarius</i> strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">33114778</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>29</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2076-2607</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>11</Issue><PubDate><Year>2020</Year><Month>Oct</Month><Day>26</Day></PubDate></JournalIssue><Title>Microorganisms</Title><ISOAbbreviation>Microorganisms</ISOAbbreviation></Journal><ArticleTitle>Selection of Immunobiotic <i>Ligilactobacillus salivarius</i> Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E1659</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/microorganisms8111659</ELocationID><Abstract><AbstractText>In this article, <i>Ligilactobacillus salivarius</i> FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine <i>L. salivarius</i> strains selected from subgroups that demonstrated different "immune" and "adhesion" phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that <i>L. salivarius</i> FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, <i>L. salivarius</i> FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several <i>L. salivarius</i> strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Binghui</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Albarracin</LastName><ForeName>Leonardo</ForeName><Initials>L</Initials><Identifier Source="ORCID">0000-0001-8216-2190</Identifier><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Scientific Computing Laboratory, Computer Science Department, Faculty of Exact Sciences and Technology, National University of Tucuman, Tucuman 4000, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Indo</LastName><ForeName>Yuhki</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arce</LastName><ForeName>Lorena</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Infection Biology Laboratory, INSIBIO-CONICET, Faculty of Medicine, University of Tucuman, Tucuman 4000, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Masumizu</LastName><ForeName>Yuki</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tomokiyo</LastName><ForeName>Mikado</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Islam</LastName><ForeName>Md Aminul</ForeName><Initials>MA</Initials><Identifier Source="ORCID">0000-0003-1979-9652</Identifier><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Garcia-Castillo</LastName><ForeName>Valeria</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ikeda-Ohtsubo</LastName><ForeName>Wakako</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nochi</LastName><ForeName>Tomonori</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morita</LastName><ForeName>Hidetoshi</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Takahashi</LastName><ForeName>Hideki</ForeName><Initials>H</Initials><Identifier Source="ORCID">0000-0001-5398-1867</Identifier><AffiliationInfo><Affiliation>Laboratory of Plant Pathology, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Plant Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kurata</LastName><ForeName>Shoichiro</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0002-0301-872X</Identifier><AffiliationInfo><Affiliation>Laboratory of Molecular Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Villena</LastName><ForeName>Julio</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0003-4941-4960</Identifier><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kitazawa</LastName><ForeName>Haruki</ForeName><Initials>H</Initials><Identifier Source="ORCID">0000-0003-4586-5553</Identifier><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>19H00965</GrantID><Agency>Japan Society for the Promotion of Science</Agency><Country></Country></Grant><Grant><GrantID>No. 01002A</GrantID><Agency>NARO Bio-oriented Technology Research Advancement Institution</Agency><Country></Country></Grant><Grant><GrantID>PICT-2016-0410</GrantID><Agency>ANPCyT-FONCyT</Agency><Country></Country></Grant><Grant><GrantID>FRiD</GrantID><Agency>Tohoku University Research Program "Frontier Research in Duo"</Agency><Country></Country></Grant><Grant><GrantID>16H06429, 16K21723, and 16H06435</GrantID><Agency>Ministry of Education, Culture, Sports, Science and Technology</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Microorganisms</MedlineTA><NlmUniqueID>101625893</NlmUniqueID><ISSNLinking>2076-2607</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Ligilactobacillus salivarius</Keyword><Keyword MajorTopicYN="N">adhesion</Keyword><Keyword MajorTopicYN="N">genomics</Keyword><Keyword MajorTopicYN="N">immunomodulation</Keyword><Keyword MajorTopicYN="N">pigs</Keyword><Keyword MajorTopicYN="N">porcine intestinal epithelial cells</Keyword><Keyword MajorTopicYN="N">wakame</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>09</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>10</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>10</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>29</Day><Hour>1</Hour><Minute>4</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>30</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33114778</ArticleId><ArticleId IdType="pii">microorganisms8111659</ArticleId><ArticleId IdType="doi">10.3390/microorganisms8111659</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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