Senna plant generates reactive oxygen species (ROS) and induces apoptosis in Hymenolepis diminuta.
Identifieur interne : 000051 ( Main/Exploration ); précédent : 000050; suivant : 000052Senna plant generates reactive oxygen species (ROS) and induces apoptosis in Hymenolepis diminuta.
Auteurs : Saptarshi Roy [Inde] ; Nikhilesh Joardar [Inde] ; Santi P Sinha Babu [Inde] ; Larisha M. Lyndem [Inde]Source :
- Molecular and biochemical parasitology [ 1872-9428 ] ; 2020.
Abstract
Like mammalian cells, helminth parasites are equipped with an array of enzymatic anti-oxidant system which has an adaptive strategy to cope up with several conditions of stress that arise from host immune response or drug treatment. Earlier, we had reported that three species of Senna, viz. S. alata, S. alexandrina and S. occidentalis leaf extracts caused severe morphological and biochemical alterations in the zoonotic parasite Hymenolepis diminuta. To understand whether the leaf extracts of the three species of Senna have any effect on the enzymatic anti-oxidant system in H.diminuta or not, the present study was investigated on the mechanism of action of these leaf extracts on the anti-oxidant system of the parasite. The viability of the parasite was assessed by MTT reduction assay, chromatin condensation through Hoechst staining of tissue and DNA fragmentation assay, and the oxidative enzymes of the parasite were estimated biochemically. Activity of superoxide dismutase, catalase, glutathione S- transferase and glutathione peroxidase were found to be increased in all the treated parasites from that of the control, with S. alata showed the highest increased amongst the three plant species in all the enzymes, at 331.0 %, 215.4 %, 85.4 % and 65.5 % respectively. Upliftment of apoptotic protein CED-3, CED-4 and EGL-1 and down regulation of anti-apototic protein CED-9 was visualised in all treated paraites. The redox imbalance triggered by these leaf extracts resulted in the activation of apoptotic pathway that led to death of the parasite. Our results demonstrated that the leaf extracts of the three Senna plant species could open new insight for an affordable natural anthelmintic with high efficacy and less toxicity.
DOI: 10.1016/j.molbiopara.2020.111297
PubMed: 32619645
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Lyndem</name><affiliation wicri:level="1"><nlm:affiliation>Parasitology Research Laboratory, Department of Zoology, Visva-Bharati, Santiniketan, 731235, West Bengal, India. Electronic address: lyndemlarisha@gmail.com.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Parasitology Research Laboratory, Department of Zoology, Visva-Bharati, Santiniketan, 731235, West Bengal</wicri:regionArea><wicri:noRegion>West Bengal</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecular and biochemical parasitology</title><idno type="eISSN">1872-9428</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Like mammalian cells, helminth parasites are equipped with an array of enzymatic anti-oxidant system which has an adaptive strategy to cope up with several conditions of stress that arise from host immune response or drug treatment. Earlier, we had reported that three species of Senna, viz. S. alata, S. alexandrina and S. occidentalis leaf extracts caused severe morphological and biochemical alterations in the zoonotic parasite Hymenolepis diminuta. To understand whether the leaf extracts of the three species of Senna have any effect on the enzymatic anti-oxidant system in H.diminuta or not, the present study was investigated on the mechanism of action of these leaf extracts on the anti-oxidant system of the parasite. The viability of the parasite was assessed by MTT reduction assay, chromatin condensation through Hoechst staining of tissue and DNA fragmentation assay, and the oxidative enzymes of the parasite were estimated biochemically. Activity of superoxide dismutase, catalase, glutathione S- transferase and glutathione peroxidase were found to be increased in all the treated parasites from that of the control, with S. alata showed the highest increased amongst the three plant species in all the enzymes, at 331.0 %, 215.4 %, 85.4 % and 65.5 % respectively. Upliftment of apoptotic protein CED-3, CED-4 and EGL-1 and down regulation of anti-apototic protein CED-9 was visualised in all treated paraites. The redox imbalance triggered by these leaf extracts resulted in the activation of apoptotic pathway that led to death of the parasite. Our results demonstrated that the leaf extracts of the three Senna plant species could open new insight for an affordable natural anthelmintic with high efficacy and less toxicity.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32619645</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>05</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-9428</ISSN><JournalIssue CitedMedium="Internet"><Volume>238</Volume><PubDate><Year>2020</Year><Month>07</Month></PubDate></JournalIssue><Title>Molecular and biochemical parasitology</Title><ISOAbbreviation>Mol Biochem Parasitol</ISOAbbreviation></Journal><ArticleTitle>Senna plant generates reactive oxygen species (ROS) and induces apoptosis in Hymenolepis diminuta.</ArticleTitle><Pagination><MedlinePgn>111297</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0166-6851(20)30061-X</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.molbiopara.2020.111297</ELocationID><Abstract><AbstractText>Like mammalian cells, helminth parasites are equipped with an array of enzymatic anti-oxidant system which has an adaptive strategy to cope up with several conditions of stress that arise from host immune response or drug treatment. Earlier, we had reported that three species of Senna, viz. S. alata, S. alexandrina and S. occidentalis leaf extracts caused severe morphological and biochemical alterations in the zoonotic parasite Hymenolepis diminuta. To understand whether the leaf extracts of the three species of Senna have any effect on the enzymatic anti-oxidant system in H.diminuta or not, the present study was investigated on the mechanism of action of these leaf extracts on the anti-oxidant system of the parasite. The viability of the parasite was assessed by MTT reduction assay, chromatin condensation through Hoechst staining of tissue and DNA fragmentation assay, and the oxidative enzymes of the parasite were estimated biochemically. Activity of superoxide dismutase, catalase, glutathione S- transferase and glutathione peroxidase were found to be increased in all the treated parasites from that of the control, with S. alata showed the highest increased amongst the three plant species in all the enzymes, at 331.0 %, 215.4 %, 85.4 % and 65.5 % respectively. Upliftment of apoptotic protein CED-3, CED-4 and EGL-1 and down regulation of anti-apototic protein CED-9 was visualised in all treated paraites. The redox imbalance triggered by these leaf extracts resulted in the activation of apoptotic pathway that led to death of the parasite. Our results demonstrated that the leaf extracts of the three Senna plant species could open new insight for an affordable natural anthelmintic with high efficacy and less toxicity.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Roy</LastName><ForeName>Saptarshi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Parasitology Research Laboratory, Department of Zoology, Visva-Bharati, Santiniketan, 731235, West Bengal, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Joardar</LastName><ForeName>Nikhilesh</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Molecular Parasitology Laboratory, Department of Zoology, Visva-Bharati, Santiniketan, 731235, West Bengal, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Babu</LastName><ForeName>Santi P Sinha</ForeName><Initials>SPS</Initials><AffiliationInfo><Affiliation>Molecular Parasitology Laboratory, Department of Zoology, Visva-Bharati, Santiniketan, 731235, West Bengal, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lyndem</LastName><ForeName>Larisha M</ForeName><Initials>LM</Initials><AffiliationInfo><Affiliation>Parasitology Research Laboratory, Department of Zoology, Visva-Bharati, Santiniketan, 731235, West Bengal, India. Electronic address: lyndemlarisha@gmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>06</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Mol Biochem Parasitol</MedlineTA><NlmUniqueID>8006324</NlmUniqueID><ISSNLinking>0166-6851</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Anthelmintic</Keyword><Keyword MajorTopicYN="Y">Anti-oxidant</Keyword><Keyword MajorTopicYN="Y">Hymenolepis diminuta</Keyword><Keyword MajorTopicYN="Y">Reactive oxygen species</Keyword><Keyword MajorTopicYN="Y">Senna</Keyword></KeywordList><CoiStatement>Declaration of Competing Interest The authors declare no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>05</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>06</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>06</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32619645</ArticleId><ArticleId IdType="pii">S0166-6851(20)30061-X</ArticleId><ArticleId IdType="doi">10.1016/j.molbiopara.2020.111297</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Roy, Saptarshi" sort="Roy, Saptarshi" uniqKey="Roy S" first="Saptarshi" last="Roy">Saptarshi Roy</name></noRegion><name sortKey="Babu, Santi P Sinha" sort="Babu, Santi P Sinha" uniqKey="Babu S" first="Santi P Sinha" last="Babu">Santi P Sinha Babu</name><name sortKey="Joardar, Nikhilesh" sort="Joardar, Nikhilesh" uniqKey="Joardar N" first="Nikhilesh" last="Joardar">Nikhilesh Joardar</name><name sortKey="Lyndem, Larisha M" sort="Lyndem, Larisha M" uniqKey="Lyndem L" first="Larisha M" last="Lyndem">Larisha M. 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