A glutathione S-transferase (BdGSTd9) participates in malathion resistance via directly depleting malathion and its toxic oxide malaoxon in Bactrocera dorsalis (Hendel).
Identifieur interne : 000238 ( Main/Exploration ); précédent : 000237; suivant : 000239A glutathione S-transferase (BdGSTd9) participates in malathion resistance via directly depleting malathion and its toxic oxide malaoxon in Bactrocera dorsalis (Hendel).
Auteurs : Li-Wei Meng [République populaire de Chine] ; Meng-Lan Peng [République populaire de Chine] ; Meng-Ling Chen [République populaire de Chine] ; Guo-Rui Yuan [République populaire de Chine] ; Li-Sha Zheng [République populaire de Chine] ; Wen-Jie Bai [République populaire de Chine] ; Guy Smagghe [République populaire de Chine, Belgique] ; Jin-Jun Wang [République populaire de Chine]Source :
- Pest management science [ 1526-4998 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- analogues et dérivés : Malathion.
- Animaux, Glutathione transferase, Insecticides, Oxydes, Tephritidae.
English descriptors
- KwdEn :
- MESH :
- chemical , analogs & derivatives : Malathion.
- chemical : Glutathione Transferase, Insecticides, Oxides.
- Animals, Tephritidae.
Abstract
BACKGROUND
The oriental fruit fly, Bactrocera dorsalis (Hendel), is a widespread agricultural pest that has evolved resistance to many commonly used insecticides including malathion. Glutathione S-transferases (GSTs) are multifunctional enzymes that metabolize insecticides directly or indirectly. The specific mechanism used by GSTs to confer malathion resistance in B. dorsalis is unclear.
RESULTS
BdGSTd9 was identified from B. dorsalis and was expressed at twice the level in a malathion-resistant strain (MR) than in a susceptible strain (MS). By using RNAi of BdGSTd9, the toxicity of malathion against MR was increased. Protein modelling and docking of BdGSTd9 with malathion and malaoxon indicated key amino acid residues for direct binding in the active site. In vitro assays with engineered Sf9 cells overexpressing BdGSTd9 demonstrated lower cytotoxicity of malathion. High performance liquid chromatography (HPLC) analysis indicated that malathion could be broken down significantly by BdGSTd9, and it also could deplete the malathion metabolite malaoxon, which possesses a higher toxicity to B. dorsalis. Taken together, the BdGSTd9 of B. dorsalis could not only deplete malathion, but also react with malaoxon and therefore enhance malathion resistance.
CONCLUSION
BdGSTd9 is a component of malathion resistance in B. dorsalis. It acts by depleting both malathion and malaoxon. © 2020 Society of Chemical Industry.
DOI: 10.1002/ps.5810
PubMed: 32128980
Affiliations:
- Belgique, République populaire de Chine
- Province de Flandre-Orientale, Région flamande
- Gand
- Université de Gand
Links toward previous steps (curation, corpus...)
Le document en format XML
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last="Meng">Li-Wei Meng</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Peng, Meng Lan" sort="Peng, Meng Lan" uniqKey="Peng M" first="Meng-Lan" last="Peng">Meng-Lan Peng</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Meng Ling" sort="Chen, Meng Ling" uniqKey="Chen M" first="Meng-Ling" last="Chen">Meng-Ling Chen</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Yuan, Guo Rui" sort="Yuan, Guo Rui" uniqKey="Yuan G" first="Guo-Rui" last="Yuan">Guo-Rui Yuan</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Li Sha" sort="Zheng, Li Sha" uniqKey="Zheng L" first="Li-Sha" last="Zheng">Li-Sha Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Bai, Wen Jie" sort="Bai, Wen Jie" uniqKey="Bai W" first="Wen-Jie" last="Bai">Wen-Jie Bai</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Smagghe, Guy" sort="Smagghe, Guy" uniqKey="Smagghe G" first="Guy" last="Smagghe">Guy Smagghe</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:affiliation>Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation></author><author><name sortKey="Wang, Jin Jun" sort="Wang, Jin Jun" uniqKey="Wang J" first="Jin-Jun" last="Wang">Jin-Jun Wang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author></analytic><series><title level="j">Pest management science</title><idno type="eISSN">1526-4998</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Glutathione Transferase (MeSH)</term><term>Insecticides (MeSH)</term><term>Malathion (analogs & derivatives)</term><term>Oxides (MeSH)</term><term>Tephritidae (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Glutathione transferase (MeSH)</term><term>Insecticides (MeSH)</term><term>Malathion (analogues et dérivés)</term><term>Oxydes (MeSH)</term><term>Tephritidae (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Malathion</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Glutathione Transferase</term><term>Insecticides</term><term>Oxides</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Malathion</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Tephritidae</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Glutathione transferase</term><term>Insecticides</term><term>Oxydes</term><term>Tephritidae</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>The oriental fruit fly, Bactrocera dorsalis (Hendel), is a widespread agricultural pest that has evolved resistance to many commonly used insecticides including malathion. Glutathione S-transferases (GSTs) are multifunctional enzymes that metabolize insecticides directly or indirectly. The specific mechanism used by GSTs to confer malathion resistance in B. dorsalis is unclear.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>BdGSTd9 was identified from B. dorsalis and was expressed at twice the level in a malathion-resistant strain (MR) than in a susceptible strain (MS). By using RNAi of BdGSTd9, the toxicity of malathion against MR was increased. Protein modelling and docking of BdGSTd9 with malathion and malaoxon indicated key amino acid residues for direct binding in the active site. In vitro assays with engineered Sf9 cells overexpressing BdGSTd9 demonstrated lower cytotoxicity of malathion. High performance liquid chromatography (HPLC) analysis indicated that malathion could be broken down significantly by BdGSTd9, and it also could deplete the malathion metabolite malaoxon, which possesses a higher toxicity to B. dorsalis. Taken together, the BdGSTd9 of B. dorsalis could not only deplete malathion, but also react with malaoxon and therefore enhance malathion resistance.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>BdGSTd9 is a component of malathion resistance in B. dorsalis. It acts by depleting both malathion and malaoxon. © 2020 Society of Chemical Industry.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">32128980</PMID><DateCompleted><Year>2020</Year><Month>09</Month><Day>08</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>08</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1526-4998</ISSN><JournalIssue CitedMedium="Internet"><Volume>76</Volume><Issue>8</Issue><PubDate><Year>2020</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Pest management science</Title><ISOAbbreviation>Pest Manag Sci</ISOAbbreviation></Journal><ArticleTitle>A glutathione S-transferase (BdGSTd9) participates in malathion resistance via directly depleting malathion and its toxic oxide malaoxon in Bactrocera dorsalis (Hendel).</ArticleTitle><Pagination><MedlinePgn>2557-2568</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/ps.5810</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The oriental fruit fly, Bactrocera dorsalis (Hendel), is a widespread agricultural pest that has evolved resistance to many commonly used insecticides including malathion. Glutathione S-transferases (GSTs) are multifunctional enzymes that metabolize insecticides directly or indirectly. The specific mechanism used by GSTs to confer malathion resistance in B. dorsalis is unclear.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">BdGSTd9 was identified from B. dorsalis and was expressed at twice the level in a malathion-resistant strain (MR) than in a susceptible strain (MS). By using RNAi of BdGSTd9, the toxicity of malathion against MR was increased. Protein modelling and docking of BdGSTd9 with malathion and malaoxon indicated key amino acid residues for direct binding in the active site. In vitro assays with engineered Sf9 cells overexpressing BdGSTd9 demonstrated lower cytotoxicity of malathion. High performance liquid chromatography (HPLC) analysis indicated that malathion could be broken down significantly by BdGSTd9, and it also could deplete the malathion metabolite malaoxon, which possesses a higher toxicity to B. dorsalis. Taken together, the BdGSTd9 of B. dorsalis could not only deplete malathion, but also react with malaoxon and therefore enhance malathion resistance.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">BdGSTd9 is a component of malathion resistance in B. dorsalis. It acts by depleting both malathion and malaoxon. © 2020 Society of Chemical Industry.</AbstractText><CopyrightInformation>© 2020 Society of Chemical Industry.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Meng</LastName><ForeName>Li-Wei</ForeName><Initials>LW</Initials><AffiliationInfo><Affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peng</LastName><ForeName>Meng-Lan</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Meng-Ling</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Guo-Rui</ForeName><Initials>GR</Initials><AffiliationInfo><Affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Li-Sha</ForeName><Initials>LS</Initials><AffiliationInfo><Affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bai</LastName><ForeName>Wen-Jie</ForeName><Initials>WJ</Initials><AffiliationInfo><Affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Smagghe</LastName><ForeName>Guy</ForeName><Initials>G</Initials><Identifier Source="ORCID">https://orcid.org/0000-0001-8334-3313</Identifier><AffiliationInfo><Affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jin-Jun</ForeName><Initials>JJ</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-8777-5268</Identifier><AffiliationInfo><Affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>Modern Agro-industry (Citrus) Technology Research System of China</Agency><Country></Country></Grant><Grant><Agency>Foundation Project of Southwest University</Agency><Country></Country></Grant><Grant><Agency>Chongqing Research Program of Basic Research and Frontier Technology</Agency><Country></Country></Grant><Grant><Agency>National Student's Program for Innovation and Entrepreneurship Training Program</Agency><Country></Country></Grant><Grant><Agency>National Key Research and Development Project</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>03</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Pest Manag Sci</MedlineTA><NlmUniqueID>100898744</NlmUniqueID><ISSNLinking>1526-498X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007306">Insecticides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010087">Oxides</NameOfSubstance></Chemical><Chemical><RegistryNumber>2439JYF84Q</RegistryNumber><NameOfSubstance UI="C100082">malaoxon</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.1.18</RegistryNumber><NameOfSubstance UI="D005982">Glutathione Transferase</NameOfSubstance></Chemical><Chemical><RegistryNumber>U5N7SU872W</RegistryNumber><NameOfSubstance UI="D008294">Malathion</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005982" MajorTopicYN="N">Glutathione Transferase</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007306" MajorTopicYN="N">Insecticides</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008294" MajorTopicYN="N">Malathion</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010087" MajorTopicYN="N">Oxides</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D033621" MajorTopicYN="Y">Tephritidae</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bactrocera dorsalis</Keyword><Keyword MajorTopicYN="N">HPLC</Keyword><Keyword MajorTopicYN="N">RNAi</Keyword><Keyword MajorTopicYN="N">glutathione S-transferases</Keyword><Keyword MajorTopicYN="N">insecticide resistance</Keyword><Keyword MajorTopicYN="N">malaoxon</Keyword><Keyword MajorTopicYN="N">malathion</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>12</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>02</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32128980</ArticleId><ArticleId IdType="doi">10.1002/ps.5810</ArticleId></ArticleIdList><ReferenceList><Title>REFERENCES</Title><Reference><Citation>Jin T, Zeng L, Lin Y, Lu Y and Liang G, Insecticide resistance of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), in mainland China. 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