Antioxidant or pro-oxidant and glutathione transferase P1-1 inhibiting activities for Tamarindus indica seeds and their cytotoxic effect on MCF-7 cancer cell line.
Identifieur interne : 000225 ( Main/Exploration ); précédent : 000224; suivant : 000226Antioxidant or pro-oxidant and glutathione transferase P1-1 inhibiting activities for Tamarindus indica seeds and their cytotoxic effect on MCF-7 cancer cell line.
Auteurs : R A Guneidy [Égypte] ; A M Gad [Égypte] ; E R Zaki [Égypte] ; F M Ibrahim [Égypte] ; A. Shokeer [Égypte]Source :
- Journal, genetic engineering & biotechnology [ 2090-5920 ] ; 2020.
Abstract
BACKGROUND
The multidrug resistance (MDR) of cancer cells is a major obstacle to cancer treatment. Glutathione S-transferase Pi (GSTP1-1) catalyzes the conjugation of glutathione with anticancer drugs and therefore reduces their efficacy. Phenolic compounds have the potential to inhibit GST P1-1 activity, which is a promising goal to overcome MDR and increase the efficacy of chemotherapy.
RESULTS
Three fractions (dichloromethane, ethyl acetate, and n-butanol) were prepared from Tamarindus indica seeds to determine their phenolic and flavonoid properties as well as their antioxidant/pro-oxidant properties. The n-butanol fraction displayed the highest levels of phenol ( 378 ± 11.7 mg gallic acid equivalent/g DW) and flavonoids (83 ± 6.0 mg rutin equivalent/g DW). Inhibiting effects on purified GSTP1-1 activity in human erythrocytes (eGST), placenta (pGST), and hGSTP1-1 have been studied. The n-butanol fraction was the most effective in inhibiting eGST, hGSTP1-1, and pGST with IC
CONCLUSIONS
However T. indica extract has a cytotoxic effect on the MCF-7 cell line; in certain situations, plant products can have an opposite effect to the intended drug, which decreases the impact of the drug.
DOI: 10.1186/s43141-020-00077-z
PubMed: 33215267
Affiliations:
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Shokeer</name><affiliation wicri:level="1"><nlm:affiliation>Molecular Biology Department, National Research Centre, Cairo, Dokki, Egypt.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Molecular Biology Department, National Research Centre, Cairo, Dokki</wicri:regionArea><wicri:noRegion>Dokki</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal, genetic engineering & biotechnology</title><idno type="eISSN">2090-5920</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>The multidrug resistance (MDR) of cancer cells is a major obstacle to cancer treatment. Glutathione S-transferase Pi (GSTP1-1) catalyzes the conjugation of glutathione with anticancer drugs and therefore reduces their efficacy. Phenolic compounds have the potential to inhibit GST P1-1 activity, which is a promising goal to overcome MDR and increase the efficacy of chemotherapy.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Three fractions (dichloromethane, ethyl acetate, and n-butanol) were prepared from Tamarindus indica seeds to determine their phenolic and flavonoid properties as well as their antioxidant/pro-oxidant properties. The n-butanol fraction displayed the highest levels of phenol ( 378 ± 11.7 mg gallic acid equivalent/g DW) and flavonoids (83 ± 6.0 mg rutin equivalent/g DW). Inhibiting effects on purified GSTP1-1 activity in human erythrocytes (eGST), placenta (pGST), and hGSTP1-1 have been studied. The n-butanol fraction was the most effective in inhibiting eGST, hGSTP1-1, and pGST with IC</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>However T. indica extract has a cytotoxic effect on the MCF-7 cell line; in certain situations, plant products can have an opposite effect to the intended drug, which decreases the impact of the drug.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">33215267</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2090-5920</ISSN><JournalIssue CitedMedium="Internet"><Volume>18</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>Nov</Month><Day>19</Day></PubDate></JournalIssue><Title>Journal, genetic engineering & biotechnology</Title><ISOAbbreviation>J Genet Eng Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Antioxidant or pro-oxidant and glutathione transferase P1-1 inhibiting activities for Tamarindus indica seeds and their cytotoxic effect on MCF-7 cancer cell line.</ArticleTitle><Pagination><MedlinePgn>74</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s43141-020-00077-z</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The multidrug resistance (MDR) of cancer cells is a major obstacle to cancer treatment. Glutathione S-transferase Pi (GSTP1-1) catalyzes the conjugation of glutathione with anticancer drugs and therefore reduces their efficacy. Phenolic compounds have the potential to inhibit GST P1-1 activity, which is a promising goal to overcome MDR and increase the efficacy of chemotherapy.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Three fractions (dichloromethane, ethyl acetate, and n-butanol) were prepared from Tamarindus indica seeds to determine their phenolic and flavonoid properties as well as their antioxidant/pro-oxidant properties. The n-butanol fraction displayed the highest levels of phenol ( 378 ± 11.7 mg gallic acid equivalent/g DW) and flavonoids (83 ± 6.0 mg rutin equivalent/g DW). Inhibiting effects on purified GSTP1-1 activity in human erythrocytes (eGST), placenta (pGST), and hGSTP1-1 have been studied. The n-butanol fraction was the most effective in inhibiting eGST, hGSTP1-1, and pGST with IC<sub>50</sub> values of 3.0 ± 0.7, 4.85 ± 0.35, and 6.6 ± 1.2 μg/ml, respectively. Cellular toxicity was investigated for the T. indica n-butanol fraction on various human cancerous cell lines. The only ones affected were MCF-7 cell lines (72%) and HePG2 (52%) indicated cytotoxicity. The value of IC<sub>50</sub> is 68.5 μg/ml of T. indica n-butanol fraction was observed compared to 1.7 μg/ml tamoxifen in MCF-7 cell lines. The combination of treatment of T. indica extract with the medicinally approved drug tamoxifen had unexpected effects; complete elimination of the cytotoxic inhibition effect of tamoxifen and the plant extract was observed.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">However T. indica extract has a cytotoxic effect on the MCF-7 cell line; in certain situations, plant products can have an opposite effect to the intended drug, which decreases the impact of the drug.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Guneidy</LastName><ForeName>R A</ForeName><Initials>RA</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-3252-7654</Identifier><AffiliationInfo><Affiliation>Molecular Biology Department, National Research Centre, Cairo, Dokki, Egypt. rasha_a_m_g@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gad</LastName><ForeName>A M</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Molecular Biology Department, National Research Centre, Cairo, Dokki, Egypt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zaki</LastName><ForeName>E R</ForeName><Initials>ER</Initials><AffiliationInfo><Affiliation>Molecular Biology Department, National Research Centre, Cairo, Dokki, Egypt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ibrahim</LastName><ForeName>F M</ForeName><Initials>FM</Initials><AffiliationInfo><Affiliation>Medicinal and Aromatic Plants Research Department, National Research Centre, Cairo, Dokki, Egypt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shokeer</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Molecular Biology Department, National Research Centre, Cairo, Dokki, Egypt.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>11010342</GrantID><Agency>National Research Centre</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Genet Eng Biotechnol</MedlineTA><NlmUniqueID>101317150</NlmUniqueID><ISSNLinking>1687-157X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Anticancer drugs</Keyword><Keyword MajorTopicYN="N">Antioxidant/pro-oxidant balance</Keyword><Keyword MajorTopicYN="N">Cancer</Keyword><Keyword MajorTopicYN="N">Glutathione transferase</Keyword><Keyword MajorTopicYN="N">Herb-drug interaction</Keyword><Keyword MajorTopicYN="N">Phenolic compounds</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>06</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>20</Day><Hour>5</Hour><Minute>51</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>21</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33215267</ArticleId><ArticleId IdType="doi">10.1186/s43141-020-00077-z</ArticleId><ArticleId IdType="pii">10.1186/s43141-020-00077-z</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Égypte</li></country></list><tree><country name="Égypte"><noRegion><name sortKey="Guneidy, R A" sort="Guneidy, R A" uniqKey="Guneidy R" first="R A" last="Guneidy">R A Guneidy</name></noRegion><name sortKey="Gad, A M" sort="Gad, A M" uniqKey="Gad A" first="A M" last="Gad">A M Gad</name><name sortKey="Ibrahim, F M" sort="Ibrahim, F M" uniqKey="Ibrahim F" first="F M" last="Ibrahim">F M Ibrahim</name><name sortKey="Shokeer, A" sort="Shokeer, A" uniqKey="Shokeer A" first="A" last="Shokeer">A. 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