Differential impact of some metal(loid)s on oxidative stress, antioxidant system, sulfur compounds, and protein profile of Indian mustard (Brassica juncea L.).
Identifieur interne : 000183 ( Main/Exploration ); précédent : 000182; suivant : 000184Differential impact of some metal(loid)s on oxidative stress, antioxidant system, sulfur compounds, and protein profile of Indian mustard (Brassica juncea L.).
Auteurs : Javed Ahmad [Inde] ; Sadia Qamar [Inde] ; Nida [Inde] ; Faheema Khan [Arabie saoudite] ; Inamul Haq [Inde] ; Asma Al-Huqail [Arabie saoudite] ; Mohammad Irfan Qureshi [Inde]Source :
- Protoplasma [ 1615-6102 ] ; 2020.
Abstract
Levels of arsenic (As), chromium (Cr), and copper (Cu) are increasing in the soils worldwide. Such contaminants cause toxicity in the plant systems which adversely affects growth and productivity. The objective of the present investigation was to elucidate individual and combined effects of As, Cr, and Cu (100 μM each) stress in metal hyper-accumulator plant Indian mustard (Brassica juncea L.), exposed for a week. The highest accumulation was in the roots and in decreasing order viz. Cu > As > Cr. The magnitude of oxidative stress was maximal in combined stress, followed by As, Cr, and then Cu stress. Glutathione in conjunction with glutathione reductase, glutathione peroxidase, and glutathione S-transferase increased in all set of stress treatments, notably when exposed to Cr alone. In addition, the level of sulfur-rich compounds like cysteine, phytochelatins, and non-protein thiols increased under each stress indicating efficient coupling of the enzyme system and sulfur-containing compounds during stress conditions. The highest tolerance or growth index of plants was recorded for Cu. Protein profiling of leaf tissues showed modulation of protein patterns in each stress. Mediator of RNA polymerase II transcription subunit 1 isoform X1, RuBisCO (large subunit), and ribosomal protein S3 proteins were more abundant under Cr and Cu stress. Zinc finger A20/AN1 domain-containing stress-associated protein 5-like protein was more abundant under Cu stress. HSP (15.7 kDa) and autophagy protein 5-like were in higher abundance under As and combined stress. Our results suggest that Indian mustard has a differential mode of defense against a particular stressor at the level of protein expression profile.
DOI: 10.1007/s00709-020-01535-8
PubMed: 32740707
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Differential impact of some metal(loid)s on oxidative stress, antioxidant system, sulfur compounds, and protein profile of Indian mustard (Brassica juncea L.).</title><author><name sortKey="Ahmad, Javed" sort="Ahmad, Javed" uniqKey="Ahmad J" first="Javed" last="Ahmad">Javed Ahmad</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025</wicri:regionArea><wicri:noRegion>110 025</wicri:noRegion></affiliation></author><author><name sortKey="Qamar, Sadia" sort="Qamar, Sadia" uniqKey="Qamar S" first="Sadia" last="Qamar">Sadia Qamar</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025</wicri:regionArea><wicri:noRegion>110 025</wicri:noRegion></affiliation></author><author><name sortKey="Nida" sort="Nida" uniqKey="Nida" last="Nida">Nida</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025</wicri:regionArea><wicri:noRegion>110 025</wicri:noRegion></affiliation></author><author><name sortKey="Khan, Faheema" sort="Khan, Faheema" uniqKey="Khan F" first="Faheema" last="Khan">Faheema Khan</name><affiliation wicri:level="1"><nlm:affiliation>Botany and Microbiology Department, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Botany and Microbiology Department, College of Science, King Saud University, Riyadh, 11451</wicri:regionArea><wicri:noRegion>11451</wicri:noRegion></affiliation></author><author><name sortKey="Haq, Inamul" sort="Haq, Inamul" uniqKey="Haq I" first="Inamul" last="Haq">Inamul Haq</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025</wicri:regionArea><wicri:noRegion>110 025</wicri:noRegion></affiliation></author><author><name sortKey="Al Huqail, Asma" sort="Al Huqail, Asma" uniqKey="Al Huqail A" first="Asma" last="Al-Huqail">Asma Al-Huqail</name><affiliation wicri:level="1"><nlm:affiliation>Botany and Microbiology Department, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Botany and Microbiology Department, College of Science, King Saud University, Riyadh, 11451</wicri:regionArea><wicri:noRegion>11451</wicri:noRegion></affiliation></author><author><name sortKey="Qureshi, Mohammad Irfan" sort="Qureshi, Mohammad Irfan" uniqKey="Qureshi M" first="Mohammad Irfan" last="Qureshi">Mohammad Irfan Qureshi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India. mirfanq@gmail.com.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025</wicri:regionArea><wicri:noRegion>110 025</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32740707</idno><idno type="pmid">32740707</idno><idno type="doi">10.1007/s00709-020-01535-8</idno><idno type="wicri:Area/Main/Corpus">000073</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000073</idno><idno type="wicri:Area/Main/Curation">000073</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000073</idno><idno type="wicri:Area/Main/Exploration">000073</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Differential impact of some metal(loid)s on oxidative stress, antioxidant system, sulfur compounds, and protein profile of Indian mustard (Brassica juncea L.).</title><author><name sortKey="Ahmad, Javed" sort="Ahmad, Javed" uniqKey="Ahmad J" first="Javed" last="Ahmad">Javed Ahmad</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025</wicri:regionArea><wicri:noRegion>110 025</wicri:noRegion></affiliation></author><author><name sortKey="Qamar, Sadia" sort="Qamar, Sadia" uniqKey="Qamar S" first="Sadia" last="Qamar">Sadia Qamar</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025</wicri:regionArea><wicri:noRegion>110 025</wicri:noRegion></affiliation></author><author><name sortKey="Nida" sort="Nida" uniqKey="Nida" last="Nida">Nida</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025</wicri:regionArea><wicri:noRegion>110 025</wicri:noRegion></affiliation></author><author><name sortKey="Khan, Faheema" sort="Khan, Faheema" uniqKey="Khan F" first="Faheema" last="Khan">Faheema Khan</name><affiliation wicri:level="1"><nlm:affiliation>Botany and Microbiology Department, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Botany and Microbiology Department, College of Science, King Saud University, Riyadh, 11451</wicri:regionArea><wicri:noRegion>11451</wicri:noRegion></affiliation></author><author><name sortKey="Haq, Inamul" sort="Haq, Inamul" uniqKey="Haq I" first="Inamul" last="Haq">Inamul Haq</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025</wicri:regionArea><wicri:noRegion>110 025</wicri:noRegion></affiliation></author><author><name sortKey="Al Huqail, Asma" sort="Al Huqail, Asma" uniqKey="Al Huqail A" first="Asma" last="Al-Huqail">Asma Al-Huqail</name><affiliation wicri:level="1"><nlm:affiliation>Botany and Microbiology Department, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Botany and Microbiology Department, College of Science, King Saud University, Riyadh, 11451</wicri:regionArea><wicri:noRegion>11451</wicri:noRegion></affiliation></author><author><name sortKey="Qureshi, Mohammad Irfan" sort="Qureshi, Mohammad Irfan" uniqKey="Qureshi M" first="Mohammad Irfan" last="Qureshi">Mohammad Irfan Qureshi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India. mirfanq@gmail.com.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025</wicri:regionArea><wicri:noRegion>110 025</wicri:noRegion></affiliation></author></analytic><series><title level="j">Protoplasma</title><idno type="eISSN">1615-6102</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Levels of arsenic (As), chromium (Cr), and copper (Cu) are increasing in the soils worldwide. Such contaminants cause toxicity in the plant systems which adversely affects growth and productivity. The objective of the present investigation was to elucidate individual and combined effects of As, Cr, and Cu (100 μM each) stress in metal hyper-accumulator plant Indian mustard (Brassica juncea L.), exposed for a week. The highest accumulation was in the roots and in decreasing order viz. Cu > As > Cr. The magnitude of oxidative stress was maximal in combined stress, followed by As, Cr, and then Cu stress. Glutathione in conjunction with glutathione reductase, glutathione peroxidase, and glutathione S-transferase increased in all set of stress treatments, notably when exposed to Cr alone. In addition, the level of sulfur-rich compounds like cysteine, phytochelatins, and non-protein thiols increased under each stress indicating efficient coupling of the enzyme system and sulfur-containing compounds during stress conditions. The highest tolerance or growth index of plants was recorded for Cu. Protein profiling of leaf tissues showed modulation of protein patterns in each stress. Mediator of RNA polymerase II transcription subunit 1 isoform X1, RuBisCO (large subunit), and ribosomal protein S3 proteins were more abundant under Cr and Cu stress. Zinc finger A20/AN1 domain-containing stress-associated protein 5-like protein was more abundant under Cu stress. HSP (15.7 kDa) and autophagy protein 5-like were in higher abundance under As and combined stress. Our results suggest that Indian mustard has a differential mode of defense against a particular stressor at the level of protein expression profile.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32740707</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1615-6102</ISSN><JournalIssue CitedMedium="Internet"><Volume>257</Volume><Issue>6</Issue><PubDate><Year>2020</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Protoplasma</Title><ISOAbbreviation>Protoplasma</ISOAbbreviation></Journal><ArticleTitle>Differential impact of some metal(loid)s on oxidative stress, antioxidant system, sulfur compounds, and protein profile of Indian mustard (Brassica juncea L.).</ArticleTitle><Pagination><MedlinePgn>1667-1683</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00709-020-01535-8</ELocationID><Abstract><AbstractText>Levels of arsenic (As), chromium (Cr), and copper (Cu) are increasing in the soils worldwide. Such contaminants cause toxicity in the plant systems which adversely affects growth and productivity. The objective of the present investigation was to elucidate individual and combined effects of As, Cr, and Cu (100 μM each) stress in metal hyper-accumulator plant Indian mustard (Brassica juncea L.), exposed for a week. The highest accumulation was in the roots and in decreasing order viz. Cu > As > Cr. The magnitude of oxidative stress was maximal in combined stress, followed by As, Cr, and then Cu stress. Glutathione in conjunction with glutathione reductase, glutathione peroxidase, and glutathione S-transferase increased in all set of stress treatments, notably when exposed to Cr alone. In addition, the level of sulfur-rich compounds like cysteine, phytochelatins, and non-protein thiols increased under each stress indicating efficient coupling of the enzyme system and sulfur-containing compounds during stress conditions. The highest tolerance or growth index of plants was recorded for Cu. Protein profiling of leaf tissues showed modulation of protein patterns in each stress. Mediator of RNA polymerase II transcription subunit 1 isoform X1, RuBisCO (large subunit), and ribosomal protein S3 proteins were more abundant under Cr and Cu stress. Zinc finger A20/AN1 domain-containing stress-associated protein 5-like protein was more abundant under Cu stress. HSP (15.7 kDa) and autophagy protein 5-like were in higher abundance under As and combined stress. Our results suggest that Indian mustard has a differential mode of defense against a particular stressor at the level of protein expression profile.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ahmad</LastName><ForeName>Javed</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qamar</LastName><ForeName>Sadia</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nida</LastName><AffiliationInfo><Affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Khan</LastName><ForeName>Faheema</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Botany and Microbiology Department, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Haq</LastName><ForeName>Inamul</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Al-Huqail</LastName><ForeName>Asma</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Botany and Microbiology Department, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qureshi</LastName><ForeName>Mohammad Irfan</ForeName><Initials>MI</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-9625-0036</Identifier><AffiliationInfo><Affiliation>Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110 025, India. mirfanq@gmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>RG-1438-036</GrantID><Agency>Deanship of Scientific Research, King Saud University (SA)</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>Austria</Country><MedlineTA>Protoplasma</MedlineTA><NlmUniqueID>9806853</NlmUniqueID><ISSNLinking>0033-183X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Indian mustard</Keyword><Keyword MajorTopicYN="N">Mass spectrometry</Keyword><Keyword MajorTopicYN="N">Metal(loid)s</Keyword><Keyword MajorTopicYN="N">Protein profiling</Keyword><Keyword MajorTopicYN="N">Sulfur-containing compounds</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>03</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>07</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32740707</ArticleId><ArticleId IdType="doi">10.1007/s00709-020-01535-8</ArticleId><ArticleId IdType="pii">10.1007/s00709-020-01535-8</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Arabie saoudite</li><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Ahmad, Javed" sort="Ahmad, Javed" uniqKey="Ahmad J" first="Javed" last="Ahmad">Javed Ahmad</name></noRegion><name sortKey="Haq, Inamul" sort="Haq, Inamul" uniqKey="Haq I" first="Inamul" last="Haq">Inamul Haq</name><name sortKey="Nida" sort="Nida" uniqKey="Nida" last="Nida">Nida</name><name sortKey="Qamar, Sadia" sort="Qamar, Sadia" uniqKey="Qamar S" first="Sadia" last="Qamar">Sadia Qamar</name><name sortKey="Qureshi, Mohammad Irfan" sort="Qureshi, Mohammad Irfan" uniqKey="Qureshi M" first="Mohammad Irfan" last="Qureshi">Mohammad Irfan Qureshi</name></country><country name="Arabie saoudite"><noRegion><name sortKey="Khan, Faheema" sort="Khan, Faheema" uniqKey="Khan F" first="Faheema" last="Khan">Faheema Khan</name></noRegion><name sortKey="Al Huqail, Asma" sort="Al Huqail, Asma" uniqKey="Al Huqail A" first="Asma" last="Al-Huqail">Asma Al-Huqail</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PlantGlutaTransV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000183 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000183 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PlantGlutaTransV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32740707
|texte= Differential impact of some metal(loid)s on oxidative stress, antioxidant system, sulfur compounds, and protein profile of Indian mustard (Brassica juncea L.).
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32740707" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PlantGlutaTransV1
| This area was generated with Dilib version V0.6.38. |  |