Nephroprotective effect of Combretum micranthum G. Don in nicotinamide-streptozotocin induced diabetic nephropathy in rats: In-vivo and in-silico experiments.
Identifieur interne : 000086 ( Main/Exploration ); précédent : 000085; suivant : 000087Nephroprotective effect of Combretum micranthum G. Don in nicotinamide-streptozotocin induced diabetic nephropathy in rats: In-vivo and in-silico experiments.
Auteurs : Mabozou Kpemissi [Inde] ; Adrian-Valentin Potârniche [Roumanie] ; Povi Lawson-Evi [Togo] ; Kossi Metowogo [Togo] ; Mamatchi Melila [Togo] ; Pare Dramane [Burkina Faso] ; Marian Taulescu [Roumanie] ; Vivek Chandramohan [Inde] ; Doddamavattur Shivalingaiah Suhas [Inde] ; Tumbadi Adinarayanashetty Puneeth [Inde] ; Vijaya Kumar S [Inde] ; Laurian Vlase [Roumanie] ; Sanda Andrei [Roumanie] ; Kwashie Eklu-Gadegbeku [Togo] ; Bogdan Sevastre [Roumanie] ; Veeresh Prabhakar Veerapur [Inde]Source :
- Journal of ethnopharmacology [ 1872-7573 ] ; 2020.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE
Combretum micranthum G. Don (CM) is extensively used in traditional medicine throughout West Africa and commonly known as "long-life herbal tea" or "plant to heal". Further, traditional healers frequently use the title plant to mitigate of renal disorders.
AIM OF THE STUDY
To explore the nephroprotective property of standardised hydroalcoholic extract of Combretum micranthum in nicotinamide-streptozotocin induced diabetic nephropathy in rats. In addition, in-silico computational experiments were performed with bioactive compounds of the title plant against PPARα and PPARγ.
MATERIAL AND METHODS
Male rats were made diabetic by a single intraperitoneal (ip) injection of STZ (50 mg/kg), 15 min after ip administration of NA (100 mg/kg) dissolved in normal saline. The diabetic rats received CM extract (200 and 400 mg/kg p.o.) daily, for eight weeks. Body weights and blood glucose (non-fasting and fasting) of rats were measured weekly. Daily food and water consumption were also measured. After 8 weeks of treatment, urine biochemical parameters such as N-Acetyl-β-D-Glucosaminidase (NAG), urea (UR), uric acid (UA), creatinine (CRE), and serum markers of diabetes, kidney damage and liver damage such as insulin, lipid parameters), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (γGT), albumin (Alb), magnesium (Mg
RESULTS
At the end of eight weeks, renal damage induced by the consequence of prolong diabetic condition was confirmed by altered levels of serum and urine kidney and liver function markers, oxidative stress markers and histopathological variations in kidney. Treatment with CM extract ameliorated the diabetes mellitus-induced renal biochemical parameters and histopathological changes. Further, HPLC-UV & MS experiments revealed that CM extract contains several bioactive compounds including hyperozide (62.35 μg/mg of extract) and quercitrin (19.07 μg/mg of extract). In-silico experiment exhibited cianidanol (-17.133), epicatechin (-15.107) exhibited higher docking score against PPARα and luteoforol (-11.038), epigallocatechin (-10.736) against PPARγ. Based on docking and drug likeness score, four bioactive compounds were selected for molecular dynamic experiments. Cianidanol and epigallocatechin out of the 30 compounds are concluded as a potential candidate for the treatment of DN through activating PPARα and PPARγ target protein.
CONCLUSIONS
Taken together, the present study provided the scientific footage for the traditional use of Combretum micranthum.
DOI: 10.1016/j.jep.2020.113133
PubMed: 32673708
Affiliations:
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Road, Tumkur, 572 102, Karnataka, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka</wicri:regionArea><wicri:noRegion>Karnataka</wicri:noRegion></affiliation></author><author><name sortKey="Puneeth, Tumbadi Adinarayanashetty" sort="Puneeth, Tumbadi Adinarayanashetty" uniqKey="Puneeth T" first="Tumbadi Adinarayanashetty" last="Puneeth">Tumbadi Adinarayanashetty Puneeth</name><affiliation wicri:level="1"><nlm:affiliation>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka</wicri:regionArea><wicri:noRegion>Karnataka</wicri:noRegion></affiliation></author><author><name sortKey="S, Vijaya Kumar" sort="S, Vijaya Kumar" uniqKey="S V" first="Vijaya Kumar" last="S">Vijaya Kumar S</name><affiliation wicri:level="1"><nlm:affiliation>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka</wicri:regionArea><wicri:noRegion>Karnataka</wicri:noRegion></affiliation></author><author><name sortKey="Vlase, Laurian" sort="Vlase, Laurian" uniqKey="Vlase L" first="Laurian" last="Vlase">Laurian Vlase</name><affiliation wicri:level="1"><nlm:affiliation>University of Medicine and Pharmacy "Iuliu Hateganu", Emil Isaac Street 13, 400023, Cluj-Napoca, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>University of Medicine and Pharmacy "Iuliu Hateganu", Emil Isaac Street 13, 400023, Cluj-Napoca</wicri:regionArea><wicri:noRegion>Cluj-Napoca</wicri:noRegion></affiliation></author><author><name sortKey="Andrei, Sanda" sort="Andrei, Sanda" uniqKey="Andrei S" first="Sanda" last="Andrei">Sanda Andrei</name><affiliation wicri:level="1"><nlm:affiliation>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca</wicri:regionArea><wicri:noRegion>Cluj-Napoca</wicri:noRegion></affiliation></author><author><name sortKey="Eklu Gadegbeku, Kwashie" sort="Eklu Gadegbeku, Kwashie" uniqKey="Eklu Gadegbeku K" first="Kwashie" last="Eklu-Gadegbeku">Kwashie Eklu-Gadegbeku</name><affiliation wicri:level="1"><nlm:affiliation>University of Lomé, Togo.</nlm:affiliation><country xml:lang="fr">Togo</country><wicri:regionArea>University of Lomé</wicri:regionArea></affiliation></author><author><name sortKey="Sevastre, Bogdan" sort="Sevastre, Bogdan" uniqKey="Sevastre B" first="Bogdan" last="Sevastre">Bogdan Sevastre</name><affiliation wicri:level="1"><nlm:affiliation>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca</wicri:regionArea><wicri:noRegion>Cluj-Napoca</wicri:noRegion></affiliation></author><author><name sortKey="Veerapur, Veeresh Prabhakar" sort="Veerapur, Veeresh Prabhakar" uniqKey="Veerapur V" first="Veeresh Prabhakar" last="Veerapur">Veeresh Prabhakar Veerapur</name><affiliation wicri:level="1"><nlm:affiliation>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka, India. Electronic address: veeresh36@gmail.com.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka</wicri:regionArea><wicri:noRegion>Karnataka</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32673708</idno><idno type="pmid">32673708</idno><idno type="doi">10.1016/j.jep.2020.113133</idno><idno type="wicri:Area/Main/Corpus">000089</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000089</idno><idno type="wicri:Area/Main/Curation">000089</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000089</idno><idno type="wicri:Area/Main/Exploration">000089</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Nephroprotective effect of Combretum micranthum G. Don in nicotinamide-streptozotocin induced diabetic nephropathy in rats: In-vivo and in-silico experiments.</title><author><name sortKey="Kpemissi, Mabozou" sort="Kpemissi, Mabozou" uniqKey="Kpemissi M" first="Mabozou" last="Kpemissi">Mabozou Kpemissi</name><affiliation wicri:level="1"><nlm:affiliation>University of Lomé, Togo; University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania; Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka, India. Electronic address: mabozou@gmail.com.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>University of Lomé, Togo; University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania; Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka</wicri:regionArea><wicri:noRegion>Karnataka</wicri:noRegion></affiliation></author><author><name sortKey="Potarniche, Adrian Valentin" sort="Potarniche, Adrian Valentin" uniqKey="Potarniche A" first="Adrian-Valentin" last="Potârniche">Adrian-Valentin Potârniche</name><affiliation wicri:level="1"><nlm:affiliation>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca</wicri:regionArea><wicri:noRegion>Cluj-Napoca</wicri:noRegion></affiliation></author><author><name sortKey="Lawson Evi, Povi" sort="Lawson Evi, Povi" uniqKey="Lawson Evi P" first="Povi" last="Lawson-Evi">Povi Lawson-Evi</name><affiliation wicri:level="1"><nlm:affiliation>University of Lomé, Togo.</nlm:affiliation><country xml:lang="fr">Togo</country><wicri:regionArea>University of Lomé</wicri:regionArea></affiliation></author><author><name sortKey="Metowogo, Kossi" sort="Metowogo, Kossi" uniqKey="Metowogo K" first="Kossi" last="Metowogo">Kossi Metowogo</name><affiliation wicri:level="1"><nlm:affiliation>University of Lomé, Togo.</nlm:affiliation><country xml:lang="fr">Togo</country><wicri:regionArea>University of Lomé</wicri:regionArea></affiliation></author><author><name sortKey="Melila, Mamatchi" sort="Melila, Mamatchi" uniqKey="Melila M" first="Mamatchi" last="Melila">Mamatchi Melila</name><affiliation wicri:level="1"><nlm:affiliation>University of Lomé, Togo.</nlm:affiliation><country xml:lang="fr">Togo</country><wicri:regionArea>University of Lomé</wicri:regionArea></affiliation></author><author><name sortKey="Dramane, Pare" sort="Dramane, Pare" uniqKey="Dramane P" first="Pare" last="Dramane">Pare Dramane</name><affiliation wicri:level="1"><nlm:affiliation>University of Ouagadougou UFR/SVT, 09 BP 848, Ouagadougou 09, Burkina Faso.</nlm:affiliation><country xml:lang="fr">Burkina Faso</country><wicri:regionArea>University of Ouagadougou UFR/SVT, 09 BP 848, Ouagadougou 09</wicri:regionArea><wicri:noRegion>Ouagadougou 09</wicri:noRegion></affiliation></author><author><name sortKey="Taulescu, Marian" sort="Taulescu, Marian" uniqKey="Taulescu M" first="Marian" last="Taulescu">Marian Taulescu</name><affiliation wicri:level="1"><nlm:affiliation>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca</wicri:regionArea><wicri:noRegion>Cluj-Napoca</wicri:noRegion></affiliation></author><author><name sortKey="Chandramohan, Vivek" sort="Chandramohan, Vivek" uniqKey="Chandramohan V" first="Vivek" last="Chandramohan">Vivek Chandramohan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Siddaganga Institute of Technology, Tumkur, 572103, Karnataka, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Siddaganga Institute of Technology, Tumkur, 572103, Karnataka</wicri:regionArea><wicri:noRegion>Karnataka</wicri:noRegion></affiliation></author><author><name sortKey="Suhas, Doddamavattur Shivalingaiah" sort="Suhas, Doddamavattur Shivalingaiah" uniqKey="Suhas D" first="Doddamavattur Shivalingaiah" last="Suhas">Doddamavattur Shivalingaiah Suhas</name><affiliation wicri:level="1"><nlm:affiliation>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka</wicri:regionArea><wicri:noRegion>Karnataka</wicri:noRegion></affiliation></author><author><name sortKey="Puneeth, Tumbadi Adinarayanashetty" sort="Puneeth, Tumbadi Adinarayanashetty" uniqKey="Puneeth T" first="Tumbadi Adinarayanashetty" last="Puneeth">Tumbadi Adinarayanashetty Puneeth</name><affiliation wicri:level="1"><nlm:affiliation>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka</wicri:regionArea><wicri:noRegion>Karnataka</wicri:noRegion></affiliation></author><author><name sortKey="S, Vijaya Kumar" sort="S, Vijaya Kumar" uniqKey="S V" first="Vijaya Kumar" last="S">Vijaya Kumar S</name><affiliation wicri:level="1"><nlm:affiliation>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka</wicri:regionArea><wicri:noRegion>Karnataka</wicri:noRegion></affiliation></author><author><name sortKey="Vlase, Laurian" sort="Vlase, Laurian" uniqKey="Vlase L" first="Laurian" last="Vlase">Laurian Vlase</name><affiliation wicri:level="1"><nlm:affiliation>University of Medicine and Pharmacy "Iuliu Hateganu", Emil Isaac Street 13, 400023, Cluj-Napoca, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>University of Medicine and Pharmacy "Iuliu Hateganu", Emil Isaac Street 13, 400023, Cluj-Napoca</wicri:regionArea><wicri:noRegion>Cluj-Napoca</wicri:noRegion></affiliation></author><author><name sortKey="Andrei, Sanda" sort="Andrei, Sanda" uniqKey="Andrei S" first="Sanda" last="Andrei">Sanda Andrei</name><affiliation wicri:level="1"><nlm:affiliation>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca</wicri:regionArea><wicri:noRegion>Cluj-Napoca</wicri:noRegion></affiliation></author><author><name sortKey="Eklu Gadegbeku, Kwashie" sort="Eklu Gadegbeku, Kwashie" uniqKey="Eklu Gadegbeku K" first="Kwashie" last="Eklu-Gadegbeku">Kwashie Eklu-Gadegbeku</name><affiliation wicri:level="1"><nlm:affiliation>University of Lomé, Togo.</nlm:affiliation><country xml:lang="fr">Togo</country><wicri:regionArea>University of Lomé</wicri:regionArea></affiliation></author><author><name sortKey="Sevastre, Bogdan" sort="Sevastre, Bogdan" uniqKey="Sevastre B" first="Bogdan" last="Sevastre">Bogdan Sevastre</name><affiliation wicri:level="1"><nlm:affiliation>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca</wicri:regionArea><wicri:noRegion>Cluj-Napoca</wicri:noRegion></affiliation></author><author><name sortKey="Veerapur, Veeresh Prabhakar" sort="Veerapur, Veeresh Prabhakar" uniqKey="Veerapur V" first="Veeresh Prabhakar" last="Veerapur">Veeresh Prabhakar Veerapur</name><affiliation wicri:level="1"><nlm:affiliation>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka, India. Electronic address: veeresh36@gmail.com.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka</wicri:regionArea><wicri:noRegion>Karnataka</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of ethnopharmacology</title><idno type="eISSN">1872-7573</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>ETHNOPHARMACOLOGICAL RELEVANCE</b></p><p>Combretum micranthum G. Don (CM) is extensively used in traditional medicine throughout West Africa and commonly known as "long-life herbal tea" or "plant to heal". Further, traditional healers frequently use the title plant to mitigate of renal disorders.</p></div><div type="abstract" xml:lang="en"><p><b>AIM OF THE STUDY</b></p><p>To explore the nephroprotective property of standardised hydroalcoholic extract of Combretum micranthum in nicotinamide-streptozotocin induced diabetic nephropathy in rats. In addition, in-silico computational experiments were performed with bioactive compounds of the title plant against PPARα and PPARγ.</p></div><div type="abstract" xml:lang="en"><p><b>MATERIAL AND METHODS</b></p><p>Male rats were made diabetic by a single intraperitoneal (ip) injection of STZ (50 mg/kg), 15 min after ip administration of NA (100 mg/kg) dissolved in normal saline. The diabetic rats received CM extract (200 and 400 mg/kg p.o.) daily, for eight weeks. Body weights and blood glucose (non-fasting and fasting) of rats were measured weekly. Daily food and water consumption were also measured. After 8 weeks of treatment, urine biochemical parameters such as N-Acetyl-β-D-Glucosaminidase (NAG), urea (UR), uric acid (UA), creatinine (CRE), and serum markers of diabetes, kidney damage and liver damage such as insulin, lipid parameters), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (γGT), albumin (Alb), magnesium (Mg</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>At the end of eight weeks, renal damage induced by the consequence of prolong diabetic condition was confirmed by altered levels of serum and urine kidney and liver function markers, oxidative stress markers and histopathological variations in kidney. Treatment with CM extract ameliorated the diabetes mellitus-induced renal biochemical parameters and histopathological changes. Further, HPLC-UV & MS experiments revealed that CM extract contains several bioactive compounds including hyperozide (62.35 μg/mg of extract) and quercitrin (19.07 μg/mg of extract). In-silico experiment exhibited cianidanol (-17.133), epicatechin (-15.107) exhibited higher docking score against PPARα and luteoforol (-11.038), epigallocatechin (-10.736) against PPARγ. Based on docking and drug likeness score, four bioactive compounds were selected for molecular dynamic experiments. Cianidanol and epigallocatechin out of the 30 compounds are concluded as a potential candidate for the treatment of DN through activating PPARα and PPARγ target protein.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Taken together, the present study provided the scientific footage for the traditional use of Combretum micranthum.</p></div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32673708</PMID><DateRevised><Year>2020</Year><Month>08</Month><Day>31</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-7573</ISSN><JournalIssue CitedMedium="Internet"><Volume>261</Volume><PubDate><Year>2020</Year><Month>Oct</Month><Day>28</Day></PubDate></JournalIssue><Title>Journal of ethnopharmacology</Title><ISOAbbreviation>J Ethnopharmacol</ISOAbbreviation></Journal><ArticleTitle>Nephroprotective effect of Combretum micranthum G. Don in nicotinamide-streptozotocin induced diabetic nephropathy in rats: In-vivo and in-silico experiments.</ArticleTitle><Pagination><MedlinePgn>113133</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0378-8741(20)33014-2</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jep.2020.113133</ELocationID><Abstract><AbstractText Label="ETHNOPHARMACOLOGICAL RELEVANCE" NlmCategory="BACKGROUND">Combretum micranthum G. Don (CM) is extensively used in traditional medicine throughout West Africa and commonly known as "long-life herbal tea" or "plant to heal". Further, traditional healers frequently use the title plant to mitigate of renal disorders.</AbstractText><AbstractText Label="AIM OF THE STUDY" NlmCategory="OBJECTIVE">To explore the nephroprotective property of standardised hydroalcoholic extract of Combretum micranthum in nicotinamide-streptozotocin induced diabetic nephropathy in rats. In addition, in-silico computational experiments were performed with bioactive compounds of the title plant against PPARα and PPARγ.</AbstractText><AbstractText Label="MATERIAL AND METHODS" NlmCategory="METHODS">Male rats were made diabetic by a single intraperitoneal (ip) injection of STZ (50 mg/kg), 15 min after ip administration of NA (100 mg/kg) dissolved in normal saline. The diabetic rats received CM extract (200 and 400 mg/kg p.o.) daily, for eight weeks. Body weights and blood glucose (non-fasting and fasting) of rats were measured weekly. Daily food and water consumption were also measured. After 8 weeks of treatment, urine biochemical parameters such as N-Acetyl-β-D-Glucosaminidase (NAG), urea (UR), uric acid (UA), creatinine (CRE), and serum markers of diabetes, kidney damage and liver damage such as insulin, lipid parameters), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (γGT), albumin (Alb), magnesium (Mg<sup>2+</sup>), calcium (Ca<sup>2+</sup>), phosphorus (P), were estimated. Blood glycosylated hemoglobin (HbA1<sub>C</sub>) were also estimated. kidney and liver were used for biochemical estimation of oxidative stress markers such as lipid peroxidation, superoxide dismutase (SOD) activity and glutathione peroxidase (GPx) activity. The kidney and pancreas were used for histopathological study. Further, HPLC chemoprofiling of CM extract and in-silico molecular simulation experiments were performed.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">At the end of eight weeks, renal damage induced by the consequence of prolong diabetic condition was confirmed by altered levels of serum and urine kidney and liver function markers, oxidative stress markers and histopathological variations in kidney. Treatment with CM extract ameliorated the diabetes mellitus-induced renal biochemical parameters and histopathological changes. Further, HPLC-UV & MS experiments revealed that CM extract contains several bioactive compounds including hyperozide (62.35 μg/mg of extract) and quercitrin (19.07 μg/mg of extract). In-silico experiment exhibited cianidanol (-17.133), epicatechin (-15.107) exhibited higher docking score against PPARα and luteoforol (-11.038), epigallocatechin (-10.736) against PPARγ. Based on docking and drug likeness score, four bioactive compounds were selected for molecular dynamic experiments. Cianidanol and epigallocatechin out of the 30 compounds are concluded as a potential candidate for the treatment of DN through activating PPARα and PPARγ target protein.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Taken together, the present study provided the scientific footage for the traditional use of Combretum micranthum.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kpemissi</LastName><ForeName>Mabozou</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>University of Lomé, Togo; University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania; Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka, India. Electronic address: mabozou@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Potârniche</LastName><ForeName>Adrian-Valentin</ForeName><Initials>AV</Initials><AffiliationInfo><Affiliation>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lawson-Evi</LastName><ForeName>Povi</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>University of Lomé, Togo.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Metowogo</LastName><ForeName>Kossi</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>University of Lomé, Togo.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Melila</LastName><ForeName>Mamatchi</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>University of Lomé, Togo.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dramane</LastName><ForeName>Pare</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>University of Ouagadougou UFR/SVT, 09 BP 848, Ouagadougou 09, Burkina Faso.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Taulescu</LastName><ForeName>Marian</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chandramohan</LastName><ForeName>Vivek</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Siddaganga Institute of Technology, Tumkur, 572103, Karnataka, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Suhas</LastName><ForeName>Doddamavattur Shivalingaiah</ForeName><Initials>DS</Initials><AffiliationInfo><Affiliation>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Puneeth</LastName><ForeName>Tumbadi Adinarayanashetty</ForeName><Initials>TA</Initials><AffiliationInfo><Affiliation>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>S</LastName><ForeName>Vijaya Kumar</ForeName><Initials>VK</Initials><AffiliationInfo><Affiliation>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vlase</LastName><ForeName>Laurian</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>University of Medicine and Pharmacy "Iuliu Hateganu", Emil Isaac Street 13, 400023, Cluj-Napoca, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Andrei</LastName><ForeName>Sanda</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eklu-Gadegbeku</LastName><ForeName>Kwashie</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>University of Lomé, Togo.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sevastre</LastName><ForeName>Bogdan</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Veerapur</LastName><ForeName>Veeresh Prabhakar</ForeName><Initials>VP</Initials><AffiliationInfo><Affiliation>Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur, 572 102, Karnataka, India. Electronic address: veeresh36@gmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>Ireland</Country><MedlineTA>J Ethnopharmacol</MedlineTA><NlmUniqueID>7903310</NlmUniqueID><ISSNLinking>0378-8741</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Combretum micranthum</Keyword><Keyword MajorTopicYN="N">Diabetic nephropathy</Keyword><Keyword MajorTopicYN="N">Molecular docking</Keyword><Keyword MajorTopicYN="N">Molecular dynamic</Keyword><Keyword MajorTopicYN="N">PPARα</Keyword><Keyword MajorTopicYN="N">PPARγ</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>01</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>06</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>06</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32673708</ArticleId><ArticleId IdType="pii">S0378-8741(20)33014-2</ArticleId><ArticleId IdType="doi">10.1016/j.jep.2020.113133</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Burkina Faso</li><li>Inde</li><li>Roumanie</li><li>Togo</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Kpemissi, Mabozou" sort="Kpemissi, Mabozou" uniqKey="Kpemissi M" first="Mabozou" last="Kpemissi">Mabozou Kpemissi</name></noRegion><name sortKey="Chandramohan, Vivek" sort="Chandramohan, Vivek" uniqKey="Chandramohan V" first="Vivek" last="Chandramohan">Vivek Chandramohan</name><name sortKey="Puneeth, Tumbadi Adinarayanashetty" sort="Puneeth, Tumbadi Adinarayanashetty" uniqKey="Puneeth T" first="Tumbadi Adinarayanashetty" last="Puneeth">Tumbadi Adinarayanashetty Puneeth</name><name sortKey="S, Vijaya Kumar" sort="S, Vijaya Kumar" uniqKey="S V" first="Vijaya Kumar" last="S">Vijaya Kumar S</name><name sortKey="Suhas, Doddamavattur Shivalingaiah" sort="Suhas, Doddamavattur Shivalingaiah" uniqKey="Suhas D" first="Doddamavattur Shivalingaiah" last="Suhas">Doddamavattur Shivalingaiah Suhas</name><name sortKey="Veerapur, Veeresh Prabhakar" sort="Veerapur, Veeresh Prabhakar" uniqKey="Veerapur V" first="Veeresh Prabhakar" last="Veerapur">Veeresh Prabhakar Veerapur</name></country><country name="Roumanie"><noRegion><name sortKey="Potarniche, Adrian Valentin" sort="Potarniche, Adrian Valentin" uniqKey="Potarniche A" first="Adrian-Valentin" last="Potârniche">Adrian-Valentin Potârniche</name></noRegion><name sortKey="Andrei, Sanda" sort="Andrei, Sanda" uniqKey="Andrei S" first="Sanda" last="Andrei">Sanda Andrei</name><name sortKey="Sevastre, Bogdan" sort="Sevastre, Bogdan" uniqKey="Sevastre B" first="Bogdan" last="Sevastre">Bogdan Sevastre</name><name sortKey="Taulescu, Marian" sort="Taulescu, Marian" uniqKey="Taulescu M" first="Marian" last="Taulescu">Marian Taulescu</name><name sortKey="Vlase, Laurian" sort="Vlase, Laurian" uniqKey="Vlase L" first="Laurian" last="Vlase">Laurian Vlase</name></country><country name="Togo"><noRegion><name sortKey="Lawson Evi, Povi" sort="Lawson Evi, Povi" uniqKey="Lawson Evi P" first="Povi" last="Lawson-Evi">Povi Lawson-Evi</name></noRegion><name sortKey="Eklu Gadegbeku, Kwashie" sort="Eklu Gadegbeku, Kwashie" uniqKey="Eklu Gadegbeku K" first="Kwashie" last="Eklu-Gadegbeku">Kwashie Eklu-Gadegbeku</name><name sortKey="Melila, Mamatchi" sort="Melila, Mamatchi" uniqKey="Melila M" first="Mamatchi" last="Melila">Mamatchi Melila</name><name sortKey="Metowogo, Kossi" sort="Metowogo, Kossi" uniqKey="Metowogo K" first="Kossi" last="Metowogo">Kossi Metowogo</name></country><country name="Burkina Faso"><noRegion><name sortKey="Dramane, Pare" sort="Dramane, Pare" uniqKey="Dramane P" first="Pare" last="Dramane">Pare Dramane</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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