Overexpression of Glutathione S-Transferase Genes in Field λ-Cyhalothrin-Resistant Population of Cydia pomonella: Reference Gene Selection and Expression Analysis.
Identifieur interne : 000156 ( Main/Curation ); précédent : 000155; suivant : 000157Overexpression of Glutathione S-Transferase Genes in Field λ-Cyhalothrin-Resistant Population of Cydia pomonella: Reference Gene Selection and Expression Analysis.
Auteurs : Zi-Han Wei [République populaire de Chine] ; Miao Liu [République populaire de Chine] ; Chao Hu [République populaire de Chine] ; Xue-Qing Yang [République populaire de Chine]Source :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2020.
Abstract
Analysis of the glutathione S-transferase (GST) gene expression in an insecticide-resistant strain of Cydia pomonella using real-time quantitative polymerase chain reaction is a key step toward more mechanism studies that require suitable reference genes with stable expression. Here, nine commonly used reference genes were selected, and their expression stabilities were analyzed. Results showed that EF-1α was the most stable reference gene in all of the experimental sets. The combinations of EF-1α and 18S, EF-1α and RPL12, and EF-1α and GAPDH were sufficient for normalization of gene expression analysis accurately in developmental stages, tissues, and larvae exposed to sublethal dose of λ-cyhalothrin, respectively. Additionally, the suitability of particular reference genes was verified by analyzing the spatiotemporal and insecticide-induced expression profiles of CpGSTe3, CpGSTd3, and CpGSTd4, which were overexpressed in a λ-cyhalothrin-resistant population from northeast China. These genes were used to confer the practicability of reference genes chosen in this study.
DOI: 10.1021/acs.jafc.0c01367
PubMed: 32348133
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Liaoning</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Miao" sort="Liu, Miao" uniqKey="Liu M" first="Miao" last="Liu">Miao Liu</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning</wicri:regionArea></affiliation></author><author><name sortKey="Hu, Chao" sort="Hu, Chao" uniqKey="Hu C" first="Chao" last="Hu">Chao Hu</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning</wicri:regionArea></affiliation></author><author><name sortKey="Yang, Xue Qing" sort="Yang, Xue Qing" uniqKey="Yang X" first="Xue-Qing" last="Yang">Xue-Qing Yang</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="eISSN">1520-5118</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Analysis of the glutathione S-transferase (GST) gene expression in an insecticide-resistant strain of <i>Cydia pomonella</i> using real-time quantitative polymerase chain reaction is a key step toward more mechanism studies that require suitable reference genes with stable expression. Here, nine commonly used reference genes were selected, and their expression stabilities were analyzed. Results showed that <i>EF-1</i>α was the most stable reference gene in all of the experimental sets. The combinations of <i>EF-1</i>α and <i>18S</i>, <i>EF-1</i>α and <i>RPL1</i>2, and <i>EF-1</i>α and <i>GAPDH</i> were sufficient for normalization of gene expression analysis accurately in developmental stages, tissues, and larvae exposed to sublethal dose of λ-cyhalothrin, respectively. Additionally, the suitability of particular reference genes was verified by analyzing the spatiotemporal and insecticide-induced expression profiles of <i>CpGSTe3</i>, <i>CpGSTd3</i>, and <i>CpGSTd4</i>, which were overexpressed in a λ-cyhalothrin-resistant population from northeast China. These genes were used to confer the practicability of reference genes chosen in this study.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32348133</PMID><DateRevised><Year>2020</Year><Month>05</Month><Day>27</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-5118</ISSN><JournalIssue CitedMedium="Internet"><Volume>68</Volume><Issue>21</Issue><PubDate><Year>2020</Year><Month>May</Month><Day>27</Day></PubDate></JournalIssue><Title>Journal of agricultural and food chemistry</Title><ISOAbbreviation>J Agric Food Chem</ISOAbbreviation></Journal><ArticleTitle>Overexpression of Glutathione S-Transferase Genes in Field λ-Cyhalothrin-Resistant Population of <i>Cydia pomonella</i>: Reference Gene Selection and Expression Analysis.</ArticleTitle><Pagination><MedlinePgn>5825-5834</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.jafc.0c01367</ELocationID><Abstract><AbstractText>Analysis of the glutathione S-transferase (GST) gene expression in an insecticide-resistant strain of <i>Cydia pomonella</i> using real-time quantitative polymerase chain reaction is a key step toward more mechanism studies that require suitable reference genes with stable expression. Here, nine commonly used reference genes were selected, and their expression stabilities were analyzed. Results showed that <i>EF-1</i>α was the most stable reference gene in all of the experimental sets. The combinations of <i>EF-1</i>α and <i>18S</i>, <i>EF-1</i>α and <i>RPL1</i>2, and <i>EF-1</i>α and <i>GAPDH</i> were sufficient for normalization of gene expression analysis accurately in developmental stages, tissues, and larvae exposed to sublethal dose of λ-cyhalothrin, respectively. Additionally, the suitability of particular reference genes was verified by analyzing the spatiotemporal and insecticide-induced expression profiles of <i>CpGSTe3</i>, <i>CpGSTd3</i>, and <i>CpGSTd4</i>, which were overexpressed in a λ-cyhalothrin-resistant population from northeast China. These genes were used to confer the practicability of reference genes chosen in this study.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Zi-Han</ForeName><Initials>ZH</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Miao</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Chao</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xue-Qing</ForeName><Initials>XQ</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-3919-8013</Identifier><AffiliationInfo><Affiliation>College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>05</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Agric Food Chem</MedlineTA><NlmUniqueID>0374755</NlmUniqueID><ISSNLinking>0021-8561</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cydia pomonella</Keyword><Keyword MajorTopicYN="N">RT-qPCR</Keyword><Keyword MajorTopicYN="N">gene expression</Keyword><Keyword MajorTopicYN="N">glutathione S-transferase</Keyword><Keyword MajorTopicYN="N">reference gene</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32348133</ArticleId><ArticleId IdType="doi">10.1021/acs.jafc.0c01367</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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