Antimicrobial activity of potato aspartic proteases (StAPs) involves membrane permeabilization.
Identifieur interne : 002084 ( Main/Exploration ); précédent : 002083; suivant : 002085Antimicrobial activity of potato aspartic proteases (StAPs) involves membrane permeabilization.
Auteurs : Julieta R. Mendieta [Argentine] ; Mariana R. Pagano [Argentine] ; Fernando F. Mu Oz [Argentine] ; Gustavo R. Daleo [Argentine] ; María G. Guevara [Argentine]Source :
- Microbiology (Reading, England) [ 1350-0872 ] ; 2006.
Descripteurs français
- KwdFr :
- Antifongiques (pharmacologie), Aspartic acid endopeptidases (pharmacologie), Concentration osmolaire (MeSH), Espèces réactives de l'oxygène (MeSH), Fusarium (effets des médicaments et des substances chimiques), Perméabilité des membranes cellulaires (MeSH), Phytophthora (effets des médicaments et des substances chimiques), Protéines végétales (pharmacologie), Relation dose-effet des médicaments (MeSH), Solanum tuberosum (enzymologie).
- MESH :
- effets des médicaments et des substances chimiques : Fusarium, Phytophthora.
- enzymologie : Solanum tuberosum.
- pharmacologie : Antifongiques, Aspartic acid endopeptidases, Protéines végétales.
- Concentration osmolaire, Espèces réactives de l'oxygène, Perméabilité des membranes cellulaires, Relation dose-effet des médicaments.
English descriptors
- KwdEn :
- Antifungal Agents (pharmacology), Aspartic Acid Endopeptidases (pharmacology), Cell Membrane Permeability (MeSH), Dose-Response Relationship, Drug (MeSH), Fusarium (drug effects), Osmolar Concentration (MeSH), Phytophthora (drug effects), Plant Proteins (pharmacology), Reactive Oxygen Species (MeSH), Solanum tuberosum (enzymology).
- MESH :
- chemical , pharmacology : Antifungal Agents, Aspartic Acid Endopeptidases, Plant Proteins.
- drug effects : Fusarium, Phytophthora.
- enzymology : Solanum tuberosum.
- Cell Membrane Permeability, Dose-Response Relationship, Drug, Osmolar Concentration, Reactive Oxygen Species.
Abstract
Solanum tuberosum aspartic proteases (StAPs) with antimicrobial activity are induced after abiotic and biotic stress. In this study the ability of StAPs to produce a direct antimicrobial effect was investigated. Viability assays demonstrated that StAPs are able to kill spores of Fusarium solani and Phytophthora infestans in a dose-dependent manner. Localization experiments with FITC-labelled StAPs proved that the proteins interact directly with the surface of spores and hyphae of F. solani and P. infestans. Moreover, incubation of spores and hyphae with StAPs resulted in membrane permeabilization, as shown by the uptake of the fluorescent dye SYTOX Green. It is concluded that the antimicrobial effect of StAPs against F. solani and P. infestans is caused by a direct interaction with the microbial surfaces followed by membrane permeabilization.
DOI: 10.1099/mic.0.28816-0
PubMed: 16804179
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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In this study the ability of StAPs to produce a direct antimicrobial effect was investigated. Viability assays demonstrated that StAPs are able to kill spores of Fusarium solani and Phytophthora infestans in a dose-dependent manner. Localization experiments with FITC-labelled StAPs proved that the proteins interact directly with the surface of spores and hyphae of F. solani and P. infestans. Moreover, incubation of spores and hyphae with StAPs resulted in membrane permeabilization, as shown by the uptake of the fluorescent dye SYTOX Green. It is concluded that the antimicrobial effect of StAPs against F. solani and P. infestans is caused by a direct interaction with the microbial surfaces followed by membrane permeabilization.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16804179</PMID><DateCompleted><Year>2006</Year><Month>08</Month><Day>23</Day></DateCompleted><DateRevised><Year>2020</Year><Month>11</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1350-0872</ISSN><JournalIssue CitedMedium="Print"><Volume>152</Volume><Issue>Pt 7</Issue><PubDate><Year>2006</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Microbiology (Reading, England)</Title><ISOAbbreviation>Microbiology (Reading)</ISOAbbreviation></Journal><ArticleTitle>Antimicrobial activity of potato aspartic proteases (StAPs) involves membrane permeabilization.</ArticleTitle><Pagination><MedlinePgn>2039-2047</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1099/mic.0.28816-0</ELocationID><Abstract><AbstractText>Solanum tuberosum aspartic proteases (StAPs) with antimicrobial activity are induced after abiotic and biotic stress. In this study the ability of StAPs to produce a direct antimicrobial effect was investigated. Viability assays demonstrated that StAPs are able to kill spores of Fusarium solani and Phytophthora infestans in a dose-dependent manner. Localization experiments with FITC-labelled StAPs proved that the proteins interact directly with the surface of spores and hyphae of F. solani and P. infestans. Moreover, incubation of spores and hyphae with StAPs resulted in membrane permeabilization, as shown by the uptake of the fluorescent dye SYTOX Green. It is concluded that the antimicrobial effect of StAPs against F. solani and P. infestans is caused by a direct interaction with the microbial surfaces followed by membrane permeabilization.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mendieta</LastName><ForeName>Julieta R</ForeName><Initials>JR</Initials><AffiliationInfo><Affiliation>Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1247, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pagano</LastName><ForeName>Mariana R</ForeName><Initials>MR</Initials><AffiliationInfo><Affiliation>Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1247, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Muñoz</LastName><ForeName>Fernando F</ForeName><Initials>FF</Initials><AffiliationInfo><Affiliation>Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1247, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Daleo</LastName><ForeName>Gustavo R</ForeName><Initials>GR</Initials><AffiliationInfo><Affiliation>Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1247, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guevara</LastName><ForeName>María G</ForeName><Initials>MG</Initials><AffiliationInfo><Affiliation>Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1247, Argentina.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Microbiology (Reading)</MedlineTA><NlmUniqueID>9430468</NlmUniqueID><ISSNLinking>1350-0872</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000935">Antifungal Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.23.-</RegistryNumber><NameOfSubstance UI="D016282">Aspartic Acid Endopeptidases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000935" MajorTopicYN="N">Antifungal Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016282" MajorTopicYN="N">Aspartic Acid Endopeptidases</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002463" MajorTopicYN="N">Cell Membrane Permeability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004305" MajorTopicYN="N">Dose-Response Relationship, Drug</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005670" MajorTopicYN="N">Fusarium</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009994" MajorTopicYN="N">Osmolar Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>6</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>8</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>6</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16804179</ArticleId><ArticleId IdType="doi">10.1099/mic.0.28816-0</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Argentine</li></country></list><tree><country name="Argentine"><noRegion><name sortKey="Mendieta, Julieta R" sort="Mendieta, Julieta R" uniqKey="Mendieta J" first="Julieta R" last="Mendieta">Julieta R. Mendieta</name></noRegion><name sortKey="Daleo, Gustavo R" sort="Daleo, Gustavo R" uniqKey="Daleo G" first="Gustavo R" last="Daleo">Gustavo R. Daleo</name><name sortKey="Guevara, Maria G" sort="Guevara, Maria G" uniqKey="Guevara M" first="María G" last="Guevara">María G. Guevara</name><name sortKey="Mu Oz, Fernando F" sort="Mu Oz, Fernando F" uniqKey="Mu Oz F" first="Fernando F" last="Mu Oz">Fernando F. Mu Oz</name><name sortKey="Pagano, Mariana R" sort="Pagano, Mariana R" uniqKey="Pagano M" first="Mariana R" last="Pagano">Mariana R. Pagano</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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