PhytophthoraV1, Main, Exploration, bibRecord, 001E42

Inhibitory effect of a defensin gene from the Andean crop maca (Lepidium meyenii) against Phytophthora infestans.

Identifieur interne : 001E42 ( Main/Exploration ); précédent : 001E41; suivant : 001E43

Inhibitory effect of a defensin gene from the Andean crop maca (Lepidium meyenii) against Phytophthora infestans.

Auteurs : Julio Solis [Pérou] ; Giuliana Medrano ; Marc Ghislain

Source :

Journal of plant physiology [ 0176-1617 ] ; 2007.

RBID : pubmed:16919367

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Cloning, Molecular (MeSH), Conserved Sequence (MeSH), DNA Primers (MeSH), DNA, Plant (genetics), Defensins (genetics), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Lepidium (genetics), Molecular Sequence Data (MeSH), Phytophthora (pathogenicity), Plant Leaves (genetics), Plant Proteins (genetics), Reverse Transcriptase Polymerase Chain Reaction (MeSH).
MESH :
- chemical , genetics : DNA, Plant, Defensins, Plant Proteins.
- chemical : DNA Primers.
- genetics : Lepidium, Plant Leaves.
- pathogenicity : Phytophthora.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Conserved Sequence, Gene Expression Regulation, Plant, Genes, Plant, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction.

Abstract

In this study, we report the isolation of a defensin gene, lm-def, isolated from the Andean crop 'maca' (Lepidium meyenii) with activity against the pathogen Phytophthora infestans responsible of late blight disease of the potato and tomato crops. The lm-def gene has been isolated by polymerase chain reaction (PCR) using degenerate primers corresponding to conserved regions of 13 plant defensin genes of the Brassicaceae family assuming that defensin genes are highly conserved among cruciferous species. The lm-def gene belongs to a small multigene family of at least 10 members possibly including pseudogenes as assessed by genomic hybridization and nucleotide sequence analyses. The deduced mature Lm-Def peptide is 51 amino acids in length and has 74-94% sequence identity with other plant defensins of the Brassicaceae family. The Lm-Def peptide was produced as a fusion protein using the pET-44a expression vector and purified using an immobilized metal ion affinity chromatography. The recombinant protein (NusA:Lm-Def) exhibited in vitro activity against P. infestans. The NusA:Lm-Def protein caused growth inhibition and hyphal damage at concentration not greater than 0.4 microM. In contrast, the NusA protein alone expressed and purified similarly did not show any activity against P. infestans. Therefore, these results indicate that the lm-def gene isolated from maca belong to the plant defensin family with activity against P. infestans. Its expression in potato, as a transgene, might help to control the late blight disease caused by P. infestans with the advantage of being of plant origin.

DOI: 10.1016/j.jplph.2006.06.002
PubMed: 16919367

Affiliations:

Pérou

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Le document en format XML

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<affiliation wicri:level="1"><nlm:affiliation>International Potato Center, Applied Biotechnology Laboratory, P.O. Box 1558, Lima 12, Peru.</nlm:affiliation>
<country xml:lang="fr">Pérou</country>
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<author><name sortKey="Medrano, Giuliana" sort="Medrano, Giuliana" uniqKey="Medrano G" first="Giuliana" last="Medrano">Giuliana Medrano</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Base Sequence (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Conserved Sequence (MeSH)</term>
<term>DNA Primers (MeSH)</term>
<term>DNA, Plant (genetics)</term>
<term>Defensins (genetics)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genes, Plant (MeSH)</term>
<term>Lepidium (genetics)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Phytophthora (pathogenicity)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Proteins (genetics)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN des plantes (génétique)</term>
<term>Amorces ADN (MeSH)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Défensines (génétique)</term>
<term>Feuilles de plante (génétique)</term>
<term>Gènes de plante (MeSH)</term>
<term>Lepidium (génétique)</term>
<term>Phytophthora (pathogénicité)</term>
<term>Protéines végétales (génétique)</term>
<term>RT-PCR (MeSH)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Séquence conservée (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Plant</term>
<term>Defensins</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lepidium</term>
<term>Plant Leaves</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des plantes</term>
<term>Défensines</term>
<term>Feuilles de plante</term>
<term>Lepidium</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Cloning, Molecular</term>
<term>Conserved Sequence</term>
<term>Gene Expression Regulation, Plant</term>
<term>Genes, Plant</term>
<term>Molecular Sequence Data</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term>
<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
<term>Gènes de plante</term>
<term>RT-PCR</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Séquence conservée</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
</keywords>
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<front><div type="abstract" xml:lang="en">In this study, we report the isolation of a defensin gene, lm-def, isolated from the Andean crop 'maca' (Lepidium meyenii) with activity against the pathogen Phytophthora infestans responsible of late blight disease of the potato and tomato crops. The lm-def gene has been isolated by polymerase chain reaction (PCR) using degenerate primers corresponding to conserved regions of 13 plant defensin genes of the Brassicaceae family assuming that defensin genes are highly conserved among cruciferous species. The lm-def gene belongs to a small multigene family of at least 10 members possibly including pseudogenes as assessed by genomic hybridization and nucleotide sequence analyses. The deduced mature Lm-Def peptide is 51 amino acids in length and has 74-94% sequence identity with other plant defensins of the Brassicaceae family. The Lm-Def peptide was produced as a fusion protein using the pET-44a expression vector and purified using an immobilized metal ion affinity chromatography. The recombinant protein (NusA:Lm-Def) exhibited in vitro activity against P. infestans. The NusA:Lm-Def protein caused growth inhibition and hyphal damage at concentration not greater than 0.4 microM. In contrast, the NusA protein alone expressed and purified similarly did not show any activity against P. infestans. Therefore, these results indicate that the lm-def gene isolated from maca belong to the plant defensin family with activity against P. infestans. Its expression in potato, as a transgene, might help to control the late blight disease caused by P. infestans with the advantage of being of plant origin.</div>
</front>
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<Abstract><AbstractText>In this study, we report the isolation of a defensin gene, lm-def, isolated from the Andean crop 'maca' (Lepidium meyenii) with activity against the pathogen Phytophthora infestans responsible of late blight disease of the potato and tomato crops. The lm-def gene has been isolated by polymerase chain reaction (PCR) using degenerate primers corresponding to conserved regions of 13 plant defensin genes of the Brassicaceae family assuming that defensin genes are highly conserved among cruciferous species. The lm-def gene belongs to a small multigene family of at least 10 members possibly including pseudogenes as assessed by genomic hybridization and nucleotide sequence analyses. The deduced mature Lm-Def peptide is 51 amino acids in length and has 74-94% sequence identity with other plant defensins of the Brassicaceae family. The Lm-Def peptide was produced as a fusion protein using the pET-44a expression vector and purified using an immobilized metal ion affinity chromatography. The recombinant protein (NusA:Lm-Def) exhibited in vitro activity against P. infestans. The NusA:Lm-Def protein caused growth inhibition and hyphal damage at concentration not greater than 0.4 microM. In contrast, the NusA protein alone expressed and purified similarly did not show any activity against P. infestans. Therefore, these results indicate that the lm-def gene isolated from maca belong to the plant defensin family with activity against P. infestans. Its expression in potato, as a transgene, might help to control the late blight disease caused by P. infestans with the advantage of being of plant origin.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Solis</LastName>
<ForeName>Julio</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>International Potato Center, Applied Biotechnology Laboratory, P.O. Box 1558, Lima 12, Peru.</Affiliation>
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Inhibitory effect of a defensin gene from the Andean crop maca (Lepidium meyenii) against Phytophthora infestans.

Inhibitory effect of a defensin gene from the Andean crop maca (Lepidium meyenii) against Phytophthora infestans.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki