Identification of Phytophthora sojae genes involved in asexual sporogenesis.
Identifieur interne : 001A91 ( Main/Exploration ); précédent : 001A90; suivant : 001A92Identification of Phytophthora sojae genes involved in asexual sporogenesis.
Auteurs : Ziying Wang [République populaire de Chine] ; Zhaoxia Wang ; Jie Shen ; Guangyue Wang ; Xiaoxi Zhu ; Hongxia LuSource :
- Journal of genetics [ 0973-7731 ] ; 2009.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Phytophthora.
- physiologie : Phytophthora.
- ADN complémentaire, Clonage moléculaire, Gènes fongiques, Hybridation d'acides nucléiques, RT-PCR, Spores fongiques, Technique de soustraction.
English descriptors
- KwdEn :
- MESH :
- chemical : DNA, Complementary.
- genetics : Phytophthora.
- physiology : Phytophthora.
- Cloning, Molecular, Genes, Fungal, Nucleic Acid Hybridization, Reverse Transcriptase Polymerase Chain Reaction, Spores, Fungal, Subtraction Technique.
Abstract
To explore the molecular mechanisms involved in asexual spore development in Phytophthora sojae, the zoospores of strain PS26 were treated with ultraviolet (UV) irradiation. After selection, a mutant progeny, termed PS26-U03, was obtained and demonstrated to exhibit no oospore production. A suppression subtractive hybridization (SSH) approach was developed to investigate differences in gene expression between PS26 and PS26-U03 during asexual sporogenesis. Of the 126 sequences chosen for examination, 39 putative unigenes were identified that exhibit high expression in PS26. These sequences are predicted to encode proteins involved in metabolism, cell cycle, protein biosynthesis, cell signalling, cell defence, and transcription regulation. Seven clones were selected for temporal expression analysis using RT-PCR based on the results of the dot-blot screens. Three of the selected genes, developmental protein DG1037 (UB88), glycoside hydrolase (UB149) and a hypothetical protein (UB145), were expressed only in PS26, whereas the transcripts of phosphatidylinositol-4-phosphate 5-kinase (UB36), FAD-dependent pyridine nucleotide-disulphide oxidoreductase (UB226) and sugar transporter (UB256) were expressed at very low levels in PS26-U03 but at high levels in PS26.
DOI: 10.1007/s12041-009-0021-2
PubMed: 19700851
Affiliations:
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uniqKey="Wang G" first="Guangyue" last="Wang">Guangyue Wang</name></author><author><name sortKey="Zhu, Xiaoxi" sort="Zhu, Xiaoxi" uniqKey="Zhu X" first="Xiaoxi" last="Zhu">Xiaoxi Zhu</name></author><author><name sortKey="Lu, Hongxia" sort="Lu, Hongxia" uniqKey="Lu H" first="Hongxia" last="Lu">Hongxia Lu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19700851</idno><idno type="pmid">19700851</idno><idno type="doi">10.1007/s12041-009-0021-2</idno><idno type="wicri:Area/Main/Corpus">001A40</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001A40</idno><idno type="wicri:Area/Main/Curation">001A40</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001A40</idno><idno type="wicri:Area/Main/Exploration">001A40</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of Phytophthora sojae genes 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Xiaoxi" sort="Zhu, Xiaoxi" uniqKey="Zhu X" first="Xiaoxi" last="Zhu">Xiaoxi Zhu</name></author><author><name sortKey="Lu, Hongxia" sort="Lu, Hongxia" uniqKey="Lu H" first="Hongxia" last="Lu">Hongxia Lu</name></author></analytic><series><title level="j">Journal of genetics</title><idno type="eISSN">0973-7731</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cloning, Molecular (MeSH)</term><term>DNA, Complementary (MeSH)</term><term>Genes, Fungal (MeSH)</term><term>Nucleic Acid Hybridization (MeSH)</term><term>Phytophthora (genetics)</term><term>Phytophthora (physiology)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Spores, Fungal (MeSH)</term><term>Subtraction Technique (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Gènes fongiques (MeSH)</term><term>Hybridation d'acides nucléiques (MeSH)</term><term>Phytophthora (génétique)</term><term>Phytophthora (physiologie)</term><term>RT-PCR (MeSH)</term><term>Spores fongiques (MeSH)</term><term>Technique de soustraction (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Genes, Fungal</term><term>Nucleic Acid Hybridization</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Spores, Fungal</term><term>Subtraction Technique</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term><term>Clonage moléculaire</term><term>Gènes fongiques</term><term>Hybridation d'acides nucléiques</term><term>RT-PCR</term><term>Spores fongiques</term><term>Technique de soustraction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To explore the molecular mechanisms involved in asexual spore development in Phytophthora sojae, the zoospores of strain PS26 were treated with ultraviolet (UV) irradiation. After selection, a mutant progeny, termed PS26-U03, was obtained and demonstrated to exhibit no oospore production. A suppression subtractive hybridization (SSH) approach was developed to investigate differences in gene expression between PS26 and PS26-U03 during asexual sporogenesis. Of the 126 sequences chosen for examination, 39 putative unigenes were identified that exhibit high expression in PS26. These sequences are predicted to encode proteins involved in metabolism, cell cycle, protein biosynthesis, cell signalling, cell defence, and transcription regulation. Seven clones were selected for temporal expression analysis using RT-PCR based on the results of the dot-blot screens. Three of the selected genes, developmental protein DG1037 (UB88), glycoside hydrolase (UB149) and a hypothetical protein (UB145), were expressed only in PS26, whereas the transcripts of phosphatidylinositol-4-phosphate 5-kinase (UB36), FAD-dependent pyridine nucleotide-disulphide oxidoreductase (UB226) and sugar transporter (UB256) were expressed at very low levels in PS26-U03 but at high levels in PS26.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19700851</PMID><DateCompleted><Year>2009</Year><Month>11</Month><Day>09</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>23</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">0973-7731</ISSN><JournalIssue CitedMedium="Internet"><Volume>88</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Journal of genetics</Title><ISOAbbreviation>J Genet</ISOAbbreviation></Journal><ArticleTitle>Identification of Phytophthora sojae genes involved in asexual sporogenesis.</ArticleTitle><Pagination><MedlinePgn>141-8</MedlinePgn></Pagination><Abstract><AbstractText>To explore the molecular mechanisms involved in asexual spore development in Phytophthora sojae, the zoospores of strain PS26 were treated with ultraviolet (UV) irradiation. After selection, a mutant progeny, termed PS26-U03, was obtained and demonstrated to exhibit no oospore production. A suppression subtractive hybridization (SSH) approach was developed to investigate differences in gene expression between PS26 and PS26-U03 during asexual sporogenesis. Of the 126 sequences chosen for examination, 39 putative unigenes were identified that exhibit high expression in PS26. These sequences are predicted to encode proteins involved in metabolism, cell cycle, protein biosynthesis, cell signalling, cell defence, and transcription regulation. Seven clones were selected for temporal expression analysis using RT-PCR based on the results of the dot-blot screens. Three of the selected genes, developmental protein DG1037 (UB88), glycoside hydrolase (UB149) and a hypothetical protein (UB145), were expressed only in PS26, whereas the transcripts of phosphatidylinositol-4-phosphate 5-kinase (UB36), FAD-dependent pyridine nucleotide-disulphide oxidoreductase (UB226) and sugar transporter (UB256) were expressed at very low levels in PS26-U03 but at high levels in PS26.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Ziying</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Department of Life Science, Hefei Teachers College, Hefei, Anhui 230061, People's Republic of China. sjywzy@yahoo.com.cn</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Zhaoxia</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Jie</ForeName><Initials>J</Initials></Author><Author 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Chine</li></country></list><tree><noCountry><name sortKey="Lu, Hongxia" sort="Lu, Hongxia" uniqKey="Lu H" first="Hongxia" last="Lu">Hongxia Lu</name><name sortKey="Shen, Jie" sort="Shen, Jie" uniqKey="Shen J" first="Jie" last="Shen">Jie Shen</name><name sortKey="Wang, Guangyue" sort="Wang, Guangyue" uniqKey="Wang G" first="Guangyue" last="Wang">Guangyue Wang</name><name sortKey="Wang, Zhaoxia" sort="Wang, Zhaoxia" uniqKey="Wang Z" first="Zhaoxia" last="Wang">Zhaoxia Wang</name><name sortKey="Zhu, Xiaoxi" sort="Zhu, Xiaoxi" uniqKey="Zhu X" first="Xiaoxi" last="Zhu">Xiaoxi Zhu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Ziying" sort="Wang, Ziying" uniqKey="Wang Z" first="Ziying" last="Wang">Ziying Wang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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