Incidence of endornaviruses in Phytophthora taxon douglasfir and Phytophthora ramorum.
Identifieur interne : 001896 ( Main/Exploration ); précédent : 001895; suivant : 001897Incidence of endornaviruses in Phytophthora taxon douglasfir and Phytophthora ramorum.
Auteurs : Zisis Kozlakidis [Royaume-Uni] ; Neil A. Brown ; Atif Jamal ; Xiyu Phoon ; Robert H A. CouttsSource :
- Virus genes [ 1572-994X ] ; 2010.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Virus à ARN.
- isolement et purification : Virus à ARN.
- virologie : Phytophthora.
- Phylogenèse.
English descriptors
- KwdEn :
- MESH :
- genetics : RNA Viruses.
- isolation & purification : RNA Viruses.
- virology : Phytophthora.
- Phylogeny.
Abstract
In this investigation, we show that four Phytophthora taxon douglasfir isolates from the USA, irrespective of their geographical location or host plant, and 20% of a representative cohort of Phytophthora ramorum isolates contain endornavirus dsRNAs. Three endornavirus-specific RT-PCR amplicons were generated by RT-PCR using dsRNA isolated from the four Phytophthora taxon douglasfir isolates and one representative Phytophthora ramorum isolate as template with oligonucleotide primers designed from the sequence of Phytophthora endornavirus 1. The amplified segments showed a very high degree of sequence similarity suggesting that the virus has gone through a population bottleneck during its emergence.
DOI: 10.1007/s11262-009-0421-7
PubMed: 19915969
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Three endornavirus-specific RT-PCR amplicons were generated by RT-PCR using dsRNA isolated from the four Phytophthora taxon douglasfir isolates and one representative Phytophthora ramorum isolate as template with oligonucleotide primers designed from the sequence of Phytophthora endornavirus 1. The amplified segments showed a very high degree of sequence similarity suggesting that the virus has gone through a population bottleneck during its emergence.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19915969</PMID><DateCompleted><Year>2010</Year><Month>02</Month><Day>25</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1572-994X</ISSN><JournalIssue CitedMedium="Internet"><Volume>40</Volume><Issue>1</Issue><PubDate><Year>2010</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Virus genes</Title><ISOAbbreviation>Virus Genes</ISOAbbreviation></Journal><ArticleTitle>Incidence of endornaviruses in Phytophthora taxon douglasfir and Phytophthora ramorum.</ArticleTitle><Pagination><MedlinePgn>130-4</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11262-009-0421-7</ELocationID><Abstract><AbstractText>In this investigation, we show that four Phytophthora taxon douglasfir isolates from the USA, irrespective of their geographical location or host plant, and 20% of a representative cohort of Phytophthora ramorum isolates contain endornavirus dsRNAs. Three endornavirus-specific RT-PCR amplicons were generated by RT-PCR using dsRNA isolated from the four Phytophthora taxon douglasfir isolates and one representative Phytophthora ramorum isolate as template with oligonucleotide primers designed from the sequence of Phytophthora endornavirus 1. 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Brown</name><name sortKey="Coutts, Robert H A" sort="Coutts, Robert H A" uniqKey="Coutts R" first="Robert H A" last="Coutts">Robert H A. Coutts</name><name sortKey="Jamal, Atif" sort="Jamal, Atif" uniqKey="Jamal A" first="Atif" last="Jamal">Atif Jamal</name><name sortKey="Phoon, Xiyu" sort="Phoon, Xiyu" uniqKey="Phoon X" first="Xiyu" last="Phoon">Xiyu Phoon</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="Kozlakidis, Zisis" sort="Kozlakidis, Zisis" uniqKey="Kozlakidis Z" first="Zisis" last="Kozlakidis">Zisis Kozlakidis</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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