Matrix approach to the simultaneous detection of multiple potato pathogens by real-time PCR.
Identifieur interne : 000725 ( Main/Exploration ); précédent : 000724; suivant : 000726Matrix approach to the simultaneous detection of multiple potato pathogens by real-time PCR.
Auteurs : M M Nikitin [Russie] ; N V Statsyuk [Russie] ; P A Frantsuzov [Russie] ; V G Dzhavakhiya [Russie] ; A G Golikov [Russie]Source :
- Journal of applied microbiology [ 1365-2672 ] ; 2018.
Descripteurs français
- KwdFr :
- Amorces ADN (génétique), Maladies des plantes (microbiologie), Maladies des plantes (virologie), Pectobacterium carotovorum (génétique), Pectobacterium carotovorum (isolement et purification), Pectobacterium carotovorum (physiologie), Phytophthora infestans (classification), Phytophthora infestans (génétique), Phytophthora infestans (isolement et purification), Ralstonia solanacearum (génétique), Ralstonia solanacearum (isolement et purification), Ralstonia solanacearum (physiologie), Réaction de polymérisation en chaine en temps réel (méthodes), Solanum tuberosum (microbiologie), Solanum tuberosum (virologie), Virus (classification), Virus (génétique), Virus (isolement et purification).
- MESH :
- génétique : Amorces ADN, Pectobacterium carotovorum, Phytophthora infestans, Ralstonia solanacearum, Virus.
- isolement et purification : Pectobacterium carotovorum, Phytophthora infestans, Ralstonia solanacearum, Virus.
- microbiologie : Maladies des plantes, Solanum tuberosum.
- méthodes : Réaction de polymérisation en chaine en temps réel.
- physiologie : Pectobacterium carotovorum, Ralstonia solanacearum.
- virologie : Maladies des plantes, Solanum tuberosum.
English descriptors
- KwdEn :
- DNA Primers (genetics), Pectobacterium carotovorum (genetics), Pectobacterium carotovorum (isolation & purification), Pectobacterium carotovorum (physiology), Phytophthora infestans (classification), Phytophthora infestans (genetics), Phytophthora infestans (isolation & purification), Plant Diseases (microbiology), Plant Diseases (virology), Ralstonia solanacearum (genetics), Ralstonia solanacearum (isolation & purification), Ralstonia solanacearum (physiology), Real-Time Polymerase Chain Reaction (methods), Solanum tuberosum (microbiology), Solanum tuberosum (virology), Viruses (classification), Viruses (genetics), Viruses (isolation & purification).
- MESH :
- chemical , genetics : DNA Primers.
- classification : Phytophthora infestans, Viruses.
- genetics : Pectobacterium carotovorum, Phytophthora infestans, Ralstonia solanacearum, Viruses.
- isolation & purification : Pectobacterium carotovorum, Phytophthora infestans, Ralstonia solanacearum, Viruses.
- methods : Real-Time Polymerase Chain Reaction.
- microbiology : Plant Diseases, Solanum tuberosum.
- physiology : Pectobacterium carotovorum, Ralstonia solanacearum.
- virology : Plant Diseases, Solanum tuberosum.
Abstract
AIM
Create a method for highly sensitive, selective, rapid and easy-to-use detection and identification of economically significant potato pathogens, including viruses, bacteria and oomycetes, be it single pathogen, or a range of various pathogens occurring simultaneously.
METHODS AND RESULTS
Test-systems for real-time PCR, operating in the unified amplification regime, have been developed for Phytophthora infestans, Pectobacterium atrosepticum, Dickeya dianthicola, Dickeya solani, Ralstonia solanacearum, Pectobacterium carotovorum, Clavibacter michiganensis subsp. sepedonicus, potato viruses Y (ordinary and necrotic forms as well as indiscriminative test system, detecting all forms), A, X, S, M, potato leaf roll virus, potato mop top virus and potato spindle tuber viroid. The test-systems (including polymerase and revertase) were immobilized and lyophilized in miniature microreactors (1·2 μl) on silicon DNA/RNA microarrays (micromatrices) to be used with a mobile AriaDNA
CONCLUSIONS
Preloaded 30-reaction micromatrices having shelf life of 3 and 6 months (for RNA- and DNA-based pathogens, respectively) at room temperature with no special conditions were successfully tested on both reference and field samples in comparison with traditional ELISA and microbiological methods, showing perfect performance and sensitivity (1 pg).
SIGNIFICANCE AND IMPACT OF THE STUDY
The accurate, rapid and user-friendly diagnostic system in a micromatrix format may significantly contribute to pathogen screening and phytopathological studies.
DOI: 10.1111/jam.13686
PubMed: 29297963
Affiliations:
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carotovorum (physiology)</term><term>Phytophthora infestans (classification)</term><term>Phytophthora infestans (genetics)</term><term>Phytophthora infestans (isolation & purification)</term><term>Plant Diseases (microbiology)</term><term>Plant Diseases (virology)</term><term>Ralstonia solanacearum (genetics)</term><term>Ralstonia solanacearum (isolation & purification)</term><term>Ralstonia solanacearum (physiology)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Solanum tuberosum (microbiology)</term><term>Solanum tuberosum (virology)</term><term>Viruses (classification)</term><term>Viruses (genetics)</term><term>Viruses (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Maladies des plantes (virologie)</term><term>Pectobacterium carotovorum (génétique)</term><term>Pectobacterium carotovorum (isolement et purification)</term><term>Pectobacterium carotovorum (physiologie)</term><term>Phytophthora infestans (classification)</term><term>Phytophthora infestans (génétique)</term><term>Phytophthora infestans (isolement et purification)</term><term>Ralstonia solanacearum (génétique)</term><term>Ralstonia solanacearum (isolement et purification)</term><term>Ralstonia solanacearum (physiologie)</term><term>Réaction de polymérisation en chaine en temps réel (méthodes)</term><term>Solanum tuberosum (microbiologie)</term><term>Solanum tuberosum (virologie)</term><term>Virus (classification)</term><term>Virus (génétique)</term><term>Virus (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Phytophthora infestans</term><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Pectobacterium carotovorum</term><term>Phytophthora infestans</term><term>Ralstonia solanacearum</term><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Amorces ADN</term><term>Pectobacterium carotovorum</term><term>Phytophthora infestans</term><term>Ralstonia solanacearum</term><term>Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Pectobacterium carotovorum</term><term>Phytophthora infestans</term><term>Ralstonia solanacearum</term><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Pectobacterium carotovorum</term><term>Phytophthora infestans</term><term>Ralstonia solanacearum</term><term>Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Réaction de polymérisation en chaine en temps réel</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Pectobacterium carotovorum</term><term>Ralstonia solanacearum</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Pectobacterium carotovorum</term><term>Ralstonia solanacearum</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Maladies des plantes</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Plant Diseases</term><term>Solanum tuberosum</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>AIM</b></p><p>Create a method for highly sensitive, selective, rapid and easy-to-use detection and identification of economically significant potato pathogens, including viruses, bacteria and oomycetes, be it single pathogen, or a range of various pathogens occurring simultaneously.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS AND RESULTS</b></p><p>Test-systems for real-time PCR, operating in the unified amplification regime, have been developed for Phytophthora infestans, Pectobacterium atrosepticum, Dickeya dianthicola, Dickeya solani, Ralstonia solanacearum, Pectobacterium carotovorum, Clavibacter michiganensis subsp. sepedonicus, potato viruses Y (ordinary and necrotic forms as well as indiscriminative test system, detecting all forms), A, X, S, M, potato leaf roll virus, potato mop top virus and potato spindle tuber viroid. The test-systems (including polymerase and revertase) were immobilized and lyophilized in miniature microreactors (1·2 μl) on silicon DNA/RNA microarrays (micromatrices) to be used with a mobile AriaDNA</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Preloaded 30-reaction micromatrices having shelf life of 3 and 6 months (for RNA- and DNA-based pathogens, respectively) at room temperature with no special conditions were successfully tested on both reference and field samples in comparison with traditional ELISA and microbiological methods, showing perfect performance and sensitivity (1 pg).</p></div><div type="abstract" xml:lang="en"><p><b>SIGNIFICANCE AND IMPACT OF THE STUDY</b></p><p>The accurate, rapid and user-friendly diagnostic system in a micromatrix format may significantly contribute to pathogen screening and phytopathological studies.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29297963</PMID><DateCompleted><Year>2018</Year><Month>06</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-2672</ISSN><JournalIssue CitedMedium="Internet"><Volume>124</Volume><Issue>3</Issue><PubDate><Year>2018</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Journal of applied microbiology</Title><ISOAbbreviation>J Appl Microbiol</ISOAbbreviation></Journal><ArticleTitle>Matrix approach to the simultaneous detection of multiple potato pathogens by real-time PCR.</ArticleTitle><Pagination><MedlinePgn>797-809</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/jam.13686</ELocationID><Abstract><AbstractText Label="AIM" NlmCategory="OBJECTIVE">Create a method for highly sensitive, selective, rapid and easy-to-use detection and identification of economically significant potato pathogens, including viruses, bacteria and oomycetes, be it single pathogen, or a range of various pathogens occurring simultaneously.</AbstractText><AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">Test-systems for real-time PCR, operating in the unified amplification regime, have been developed for Phytophthora infestans, Pectobacterium atrosepticum, Dickeya dianthicola, Dickeya solani, Ralstonia solanacearum, Pectobacterium carotovorum, Clavibacter michiganensis subsp. sepedonicus, potato viruses Y (ordinary and necrotic forms as well as indiscriminative test system, detecting all forms), A, X, S, M, potato leaf roll virus, potato mop top virus and potato spindle tuber viroid. The test-systems (including polymerase and revertase) were immobilized and lyophilized in miniature microreactors (1·2 μl) on silicon DNA/RNA microarrays (micromatrices) to be used with a mobile AriaDNA<sup>®</sup> amplifier.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Preloaded 30-reaction micromatrices having shelf life of 3 and 6 months (for RNA- and DNA-based pathogens, respectively) at room temperature with no special conditions were successfully tested on both reference and field samples in comparison with traditional ELISA and microbiological methods, showing perfect performance and sensitivity (1 pg).</AbstractText><AbstractText Label="SIGNIFICANCE AND IMPACT OF THE STUDY" NlmCategory="CONCLUSIONS">The accurate, rapid and user-friendly diagnostic system in a micromatrix format may significantly contribute to pathogen screening and phytopathological studies.</AbstractText><CopyrightInformation>© 2018 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of The Society for Applied Microbiology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nikitin</LastName><ForeName>M M</ForeName><Initials>MM</Initials><AffiliationInfo><Affiliation>GenBit LLC, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Statsyuk</LastName><ForeName>N V</ForeName><Initials>NV</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-6159-148X</Identifier><AffiliationInfo><Affiliation>All-Russian Research Institute of Phytopathology, Bolshie Vyazemy, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Frantsuzov</LastName><ForeName>P A</ForeName><Initials>PA</Initials><AffiliationInfo><Affiliation>GenBit LLC, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dzhavakhiya</LastName><ForeName>V G</ForeName><Initials>VG</Initials><AffiliationInfo><Affiliation>All-Russian Research Institute of Phytopathology, Bolshie Vyazemy, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Golikov</LastName><ForeName>A G</ForeName><Initials>AG</Initials><AffiliationInfo><Affiliation>GenBit LLC, Moscow, Russia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>02</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Appl Microbiol</MedlineTA><NlmUniqueID>9706280</NlmUniqueID><ISSNLinking>1364-5072</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016973" MajorTopicYN="N">Pectobacterium carotovorum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D043368" MajorTopicYN="N">Ralstonia solanacearum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014780" MajorTopicYN="N">Viruses</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">DNA/RNA microarrays</Keyword><Keyword MajorTopicYN="N">identification</Keyword><Keyword MajorTopicYN="N">micromatrix format</Keyword><Keyword MajorTopicYN="N">potato pathogens</Keyword><Keyword MajorTopicYN="N">rapid diagnostics</Keyword><Keyword MajorTopicYN="N">real-time PCR</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>10</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>12</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>12</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>1</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>6</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>1</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29297963</ArticleId><ArticleId IdType="doi">10.1111/jam.13686</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Russie</li></country><region><li>District fédéral central</li></region><settlement><li>Moscou</li></settlement></list><tree><country name="Russie"><region name="District fédéral central"><name sortKey="Nikitin, M M" sort="Nikitin, M M" uniqKey="Nikitin M" first="M M" last="Nikitin">M M Nikitin</name></region><name sortKey="Dzhavakhiya, V G" sort="Dzhavakhiya, V G" uniqKey="Dzhavakhiya V" first="V G" last="Dzhavakhiya">V G Dzhavakhiya</name><name sortKey="Frantsuzov, P A" sort="Frantsuzov, P A" uniqKey="Frantsuzov P" first="P A" last="Frantsuzov">P A Frantsuzov</name><name sortKey="Golikov, A G" sort="Golikov, A G" uniqKey="Golikov A" first="A G" last="Golikov">A G Golikov</name><name sortKey="Statsyuk, N V" sort="Statsyuk, N V" uniqKey="Statsyuk N" first="N V" last="Statsyuk">N V Statsyuk</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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